Sutacimig / 8cn9

A4DAB249_8cn9_L_K | Source PDB: 8cn9 | Chains: L, K | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7055
Max Score
1.3707
Min Score
-3.6213
Total Score
-302.66
Score CDR
-0.3436
Score non-CDR
-0.7522

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSQTLSLTCTVSGYSISSDSAWSWIRQPPGKGLEWIGYIQYSGSTNYNPSLKSRVTISRDTSKNQFSLKLSSVTAADTAVYYCARSVNYYGNSFAVGYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKEIVLTQSPATLSLSPGERATLSCRASQGISDYLHWYQQKPGEAPRLLIKYTSQPATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQNGHSFPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) L (Heavy), K (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6213
Maximum Score 1.3707
Average Score -0.7055
Total Score -302.6646

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q L -1.5347 Soluble
2 V L 0.0000 Buried
3 Q L -1.8846 Soluble
4 L L 0.0000 Buried
5 Q L -1.8919 Soluble
6 E L -1.1963 Soluble
7 S L -0.7719 Soluble
8 G L -0.7134 Soluble
9 P L -0.5985 Soluble
11 G L 0.0000 Buried
12 L L -0.3481 Soluble
13 V L 0.0000 Buried
14 K L -2.1789 Soluble
15 P L -1.5211 Soluble
16 S L -1.3293 Soluble
17 Q L -1.8388 Soluble
18 T L -1.6444 Soluble
19 L L 0.0000 Buried
20 S L -0.8877 Soluble
21 L L 0.0000 Buried
22 T L -0.9281 Soluble
23 C L 0.0000 Buried
24 T L -1.3379 Soluble
25 V L 0.0000 Buried
26 S L -1.2336 Soluble
27 G L -1.0471 Soluble
28 Y L -0.5601 Soluble
29 S L -0.4292 Soluble
30 I L 0.0000 Buried
31 S L -0.7303 Soluble
35 S L -0.8813 Soluble
36 D L -1.5527 Soluble
37 S L 0.0000 Buried
38 A L 0.0000 Buried
39 W L 0.0000 Buried
40 S L 0.0000 Buried
41 W L 0.0000 Buried
42 I L 0.0000 Buried
43 R L 0.0000 Buried
44 Q L -0.6466 Soluble
45 P L -0.6725 Soluble
46 P L -0.7191 Soluble
47 G L -1.0569 Soluble
48 K L -1.6205 Soluble
49 G L -1.2751 Soluble
50 L L 0.0000 Buried
51 E L -1.0475 Soluble
52 W L 0.0000 Buried
53 I L 0.0000 Buried
54 G L 0.0000 Buried
55 Y L 0.0000 Buried
56 I L 0.0000 Buried
57 Q L -0.4656 Soluble
58 Y L -0.3046 Soluble
59 S L -0.2840 Soluble
63 G L -0.4784 Soluble
64 S L -0.4571 Soluble
65 T L -0.5551 Soluble
66 N L -1.0756 Soluble
67 Y L -0.9584 Soluble
68 N L 0.0000 Buried
69 P L -1.3997 Soluble
70 S L -1.1434 Soluble
71 L L -1.4735 Soluble
72 K L -2.4681 Soluble
74 S L -1.8925 Soluble
75 R L -2.7043 Soluble
76 V L 0.0000 Buried
77 T L -1.4339 Soluble
78 I L 0.0000 Buried
79 S L -1.3860 Soluble
80 R L -2.1805 Soluble
81 D L -2.0704 Soluble
82 T L -1.5728 Soluble
83 S L -1.5470 Soluble
84 K L -2.4233 Soluble
85 N L -1.8160 Soluble
86 Q L -1.7899 Soluble
87 F L 0.0000 Buried
88 S L -1.1195 Soluble
89 L L 0.0000 Buried
90 K L -2.1092 Soluble
91 L L 0.0000 Buried
92 S L -1.6112 Soluble
93 S L -1.3864 Soluble
94 V L 0.0000 Buried
95 T L -0.6460 Soluble
96 A L -0.2575 Soluble
97 A L -0.0836 Soluble
98 D L -0.2463 Soluble
99 T L -0.0592 Soluble
100 A L 0.0000 Buried
101 V L 0.1441 Aggregation-prone
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 A L 0.0000 Buried
106 R L -0.0249 Soluble
107 S L 0.0000 Buried
108 V L 0.0437 Aggregation-prone
109 N L -0.0961 Soluble
110 Y L 0.0661 Aggregation-prone
111 Y L 0.6960 Aggregation-prone
111A G L -0.2814 Soluble
112A N L -1.1281 Soluble
112 S L 0.0000 Buried
113 F L 0.0000 Buried
114 A L 0.0000 Buried
115 V L 0.0000 Buried
116 G L -0.0515 Soluble
117 Y L 0.0597 Aggregation-prone
118 W L -0.6625 Soluble
119 G L 0.0000 Buried
120 Q L -2.0958 Soluble
121 G L -1.1482 Soluble
122 T L -0.6668 Soluble
123 L L 0.0998 Aggregation-prone
124 V L 0.0000 Buried
125 T L 0.0000 Buried
126 V L 0.0000 Buried
127 S L -0.6916 Soluble
128 S L -0.7900 Soluble
129 A L -0.6050 Soluble
130 S L -0.7728 Soluble
131 T L -1.1536 Soluble
132 K L -1.9461 Soluble
133 G L -2.0324 Soluble
134 P L 0.0000 Buried
135 S L -0.9628 Soluble
136 V L 0.0000 Buried
137 F L 0.0000 Buried
138 P L -0.7245 Soluble
139 L L 0.0000 Buried
140 A L -0.0495 Soluble
141 P L -0.5290 Soluble
142 C L -0.9663 Soluble
143 S L -1.4431 Soluble
144 R L -2.0123 Soluble
145 S L -1.0888 Soluble
146 T L -0.7540 Soluble
147 S L -0.3792 Soluble
148 T L -0.3394 Soluble
149 A L -0.3552 Soluble
150 A L 0.0000 Buried
151 L L 0.3493 Aggregation-prone
152 G L 0.0000 Buried
153 C L 0.0000 Buried
154 L L 0.0000 Buried
155 V L 0.0000 Buried
156 K L -0.7329 Soluble
157 D L -1.3487 Soluble
158 Y L 0.0000 Buried
159 F L -0.4609 Soluble
160 P L 0.0000 Buried
161 E L -0.4137 Soluble
162 P L -0.6649 Soluble
163 V L 0.0000 Buried
164 T L -0.6762 Soluble
165 V L 0.0000 Buried
166 S L -0.5819 Soluble
167 W L 0.0000 Buried
168 N L -0.4689 Soluble
169 S L -0.6237 Soluble
170 G L -0.5906 Soluble
171 A L -0.2060 Soluble
172 L L -0.0105 Soluble
173 T L -0.1802 Soluble
174 S L -0.1615 Soluble
175 G L -0.1270 Soluble
176 V L 0.1777 Aggregation-prone
177 H L -0.3707 Soluble
178 T L -0.0661 Soluble
179 F L 0.0000 Buried
180 P L -0.2313 Soluble
181 A L 0.3799 Aggregation-prone
182 V L 0.8650 Aggregation-prone
183 L L 1.3707 Aggregation-prone
184 Q L 0.2546 Aggregation-prone
185 S L -0.0933 Soluble
186 S L -0.3233 Soluble
187 G L -0.0443 Soluble
188 L L -0.0609 Soluble
189 Y L 0.4696 Aggregation-prone
190 S L 0.0000 Buried
191 L L 0.0000 Buried
192 S L 0.0000 Buried
193 S L 0.0000 Buried
194 V L 0.0000 Buried
195 V L 0.0000 Buried
196 T L -0.0899 Soluble
197 V L 0.0000 Buried
198 P L -0.3630 Soluble
199 S L -0.5478 Soluble
200 S L -0.5386 Soluble
201 S L -0.5726 Soluble
202 L L -0.2905 Soluble
203 G L -0.7500 Soluble
204 T L -0.9076 Soluble
205 K L -1.5005 Soluble
206 T L -0.7227 Soluble
207 Y L -0.3158 Soluble
208 T L -0.8172 Soluble
209 C L 0.0000 Buried
210 N L -1.4061 Soluble
211 V L 0.0000 Buried
212 D L -2.2471 Soluble
213 H L 0.0000 Buried
214 K L -2.7801 Soluble
215 P L -1.7105 Soluble
216 S L -2.0424 Soluble
217 N L -2.6762 Soluble
218 T L -2.2018 Soluble
219 K L -2.7922 Soluble
220 V L -1.5010 Soluble
221 D L -2.0489 Soluble
222 K L -1.9003 Soluble
1 E K -1.4669 Soluble
2 I K 0.0000 Buried
3 V K 0.6538 Aggregation-prone
4 L K 0.0000 Buried
5 T K -0.6188 Soluble
6 Q K 0.0000 Buried
7 S K -0.6529 Soluble
8 P K -0.3897 Soluble
9 A K -0.4236 Soluble
10 T K -0.4770 Soluble
11 L K -0.2844 Soluble
12 S K -0.6166 Soluble
13 L K -0.8965 Soluble
14 S K -1.4848 Soluble
15 P K -1.8614 Soluble
16 G K -1.7511 Soluble
17 E K -2.3634 Soluble
18 R K -2.5514 Soluble
19 A K 0.0000 Buried
20 T K -0.5700 Soluble
21 L K 0.0000 Buried
22 S K -0.9853 Soluble
23 C K 0.0000 Buried
24 R K -2.3987 Soluble
25 A K 0.0000 Buried
26 S K -1.0754 Soluble
27 Q K -1.7666 Soluble
28 G K -1.6118 Soluble
29 I K 0.0000 Buried
36 S K -0.8297 Soluble
37 D K -0.5870 Soluble
38 Y K 0.3219 Aggregation-prone
39 L K 0.0000 Buried
40 H K 0.0000 Buried
41 W K 0.0000 Buried
42 Y K 0.0000 Buried
43 Q K 0.0000 Buried
44 Q K 0.0000 Buried
45 K K -2.1172 Soluble
46 P K -1.7857 Soluble
47 G K -1.9992 Soluble
48 E K -2.7857 Soluble
49 A K 0.0000 Buried
50 P K 0.0000 Buried
51 R K -1.2773 Soluble
52 L K -0.3641 Soluble
53 L K 0.0000 Buried
54 I K 0.0000 Buried
55 K K -0.6176 Soluble
56 Y K -0.2777 Soluble
57 T K -0.6017 Soluble
65 S K -0.9786 Soluble
66 Q K -1.2817 Soluble
67 P K -0.8265 Soluble
68 A K -0.6234 Soluble
69 T K -0.4065 Soluble
70 G K -0.3869 Soluble
71 I K -0.2010 Soluble
72 P K -0.1968 Soluble
74 A K -0.1980 Soluble
75 R K -0.7013 Soluble
76 F K 0.0000 Buried
77 S K -0.5350 Soluble
78 G K -0.5955 Soluble
79 S K -0.8644 Soluble
80 G K -1.0632 Soluble
83 S K -0.9744 Soluble
84 G K -1.4275 Soluble
85 T K -2.0342 Soluble
86 D K -2.4010 Soluble
87 F K 0.0000 Buried
88 T K -0.8094 Soluble
89 L K 0.0000 Buried
90 T K -0.6959 Soluble
91 I K 0.0000 Buried
92 S K -1.4361 Soluble
93 S K -1.5077 Soluble
94 L K 0.0000 Buried
95 E K -2.4231 Soluble
96 P K -2.6760 Soluble
97 E K -3.0570 Soluble
98 D K 0.0000 Buried
99 F K 0.0000 Buried
100 A K 0.0000 Buried
101 V K 0.0000 Buried
102 Y K 0.0000 Buried
103 Y K 0.0000 Buried
104 C K 0.0000 Buried
105 Q K 0.0000 Buried
106 N K 0.0000 Buried
107 G K 0.0000 Buried
108 H K -0.6442 Soluble
109 S K -0.6564 Soluble
114 F K -0.4631 Soluble
115 P K 0.0000 Buried
116 L K 0.0000 Buried
117 T K -0.2797 Soluble
118 F K 0.0000 Buried
119 G K 0.0000 Buried
120 Q K -1.3200 Soluble
121 G K 0.0000 Buried
122 T K 0.0000 Buried
123 K K -1.2127 Soluble
124 L K 0.0000 Buried
125 E K -1.7273 Soluble
126 I K 0.0000 Buried
127 K K -1.3009 Soluble
128 R K -0.9005 Soluble
129 T K 0.2404 Aggregation-prone
130 V K 1.3162 Aggregation-prone
131 A K 0.3352 Aggregation-prone
132 A K 0.0627 Aggregation-prone
133 P K 0.0000 Buried
134 S K -0.1357 Soluble
135 V K 0.0000 Buried
136 F K -0.1244 Soluble
137 I K 0.0000 Buried
138 F K 0.0000 Buried
139 P K -0.8864 Soluble
140 P K -0.7550 Soluble
141 S K -1.3511 Soluble
142 D K -2.6061 Soluble
143 E K -2.4738 Soluble
144 Q K 0.0000 Buried
145 L K -1.9423 Soluble
146 K K -2.5089 Soluble
147 S K -1.5894 Soluble
148 G K -1.0894 Soluble
149 T K -0.9023 Soluble
150 A K 0.0000 Buried
151 S K 0.0000 Buried
152 V K 0.0000 Buried
153 V K 0.0000 Buried
154 C K 0.0000 Buried
155 L K 0.0000 Buried
156 L K 0.0000 Buried
157 N K -0.5473 Soluble
158 N K -1.0598 Soluble
159 F K 0.0000 Buried
160 Y K -0.6900 Soluble
161 P K -1.3065 Soluble
162 R K -2.5477 Soluble
163 E K -3.0391 Soluble
164 A K -2.3073 Soluble
165 K K -2.4137 Soluble
166 V K -1.1170 Soluble
167 Q K -0.6182 Soluble
168 W K 0.0000 Buried
169 K K -0.6893 Soluble
170 V K 0.0000 Buried
171 D K -1.2916 Soluble
172 N K -1.2903 Soluble
173 A K -0.3658 Soluble
174 L K 0.3617 Aggregation-prone
175 Q K -0.2430 Soluble
176 S K -0.4498 Soluble
177 G K -0.7242 Soluble
178 N K -0.8161 Soluble
179 S K -0.9006 Soluble
180 Q K -0.7796 Soluble
181 E K -1.3761 Soluble
182 S K -0.2621 Soluble
183 V K -0.4645 Soluble
184 T K -0.8629 Soluble
185 E K -2.1177 Soluble
186 Q K 0.0000 Buried
187 D K -2.1820 Soluble
188 S K -2.6101 Soluble
189 K K -2.8743 Soluble
190 D K -2.1932 Soluble
191 S K 0.0000 Buried
192 T K 0.0000 Buried
193 Y K 0.0000 Buried
194 S K 0.0000 Buried
195 L K 0.0000 Buried
196 S K 0.0000 Buried
197 S K 0.0000 Buried
198 T K -0.4158 Soluble
199 L K 0.0000 Buried
200 T K -0.4819 Soluble
201 L K 0.0000 Buried
202 S K -0.9201 Soluble
203 K K -1.9306 Soluble
204 A K -1.6860 Soluble
205 D K -2.3220 Soluble
206 Y K -2.6541 Soluble
207 E K -3.6213 Soluble
208 K K -3.4280 Soluble
209 H K -2.8690 Soluble
210 K K -3.0164 Soluble
211 V K -1.6636 Soluble
212 Y K 0.0000 Buried
213 A K -0.6159 Soluble
214 C K 0.0000 Buried
215 E K -0.6571 Soluble
216 V K 0.0000 Buried
217 T K -1.0118 Soluble
218 H K 0.0000 Buried
219 Q K -1.4886 Soluble
220 G K -0.5185 Soluble
221 L K -0.2941 Soluble
222 S K -0.4220 Soluble
223 S K -0.3551 Soluble
224 P K -0.2561 Soluble
225 V K 0.2363 Aggregation-prone
226 T K -0.3440 Soluble
227 K K -0.5805 Soluble
228 S K -0.7618 Soluble
229 F K -1.6452 Soluble
230 N K -2.7314 Soluble
231 R K -3.4313 Soluble
232 G K -2.9241 Soluble
233 E K -2.9347 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7637 1.7738 CSV PDB
4.5 -0.7982 1.7701 CSV PDB
5.0 -0.8403 1.7669 CSV PDB
5.5 -0.8803 1.7667 CSV PDB
6.0 -0.9072 1.7747 CSV PDB
6.5 -0.9129 1.7981 CSV PDB
7.0 -0.8972 1.8381 CSV PDB
7.5 -0.8665 1.8888 CSV PDB
8.0 -0.8275 1.9443 CSV PDB
8.5 -0.7832 2.0018 CSV PDB
9.0 -0.7343 2.0606 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH