Elgemtumab / 4p59

A4DAB099_4p59_H_L | Source PDB: 4p59 | Chains: H, L | Done
98.66
Yes
10 Å
Yes
Avg A4D Score
-0.6673
Max Score
1.6674
Min Score
-3.8118
Total Score
-280.92
Score CDR
-0.4209
Score non-CDR
-0.696

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAINSQGKSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARWGDEGFDIWGQGTLVTVSSASTKGPSVFPLAPSSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQGISNWLAWYQQKPGKAPKLLIYGASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFAVYYCQQYSSFPTTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8118
Maximum Score 1.6674
Average Score -0.6673
Total Score -280.9179

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.0373 Aggregation-prone
3 Q H -0.2193 Soluble
4 L H 0.0000 Buried
5 V H 0.8360 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4334 Soluble
8 G H -0.9451 Soluble
9 G H -0.4929 Soluble
11 G H -0.3955 Soluble
12 L H -0.1858 Soluble
13 V H 0.0000 Buried
14 Q H -1.7036 Soluble
15 P H -1.7677 Soluble
16 G H -1.5066 Soluble
17 G H -1.1897 Soluble
18 S H -1.0768 Soluble
19 L H -1.0993 Soluble
20 R H -2.1458 Soluble
21 L H 0.0000 Buried
22 S H -0.4641 Soluble
23 C H 0.0000 Buried
24 A H -0.1750 Soluble
25 A H 0.0000 Buried
26 S H -0.1388 Soluble
27 G H 0.0313 Aggregation-prone
28 F H 0.1940 Aggregation-prone
29 T H -0.0305 Soluble
30 F H 0.0000 Buried
35 S H -0.7753 Soluble
36 S H -0.3869 Soluble
37 Y H -0.1720 Soluble
38 A H -0.3442 Soluble
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8435 Soluble
45 A H -1.1824 Soluble
46 P H -1.0912 Soluble
47 G H -1.4885 Soluble
48 K H -2.3519 Soluble
49 G H -1.5688 Soluble
50 L H 0.0000 Buried
51 E H -1.1937 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.4261 Aggregation-prone
56 I H 0.0000 Buried
57 N H -1.1306 Soluble
58 S H -1.1628 Soluble
59 Q H -2.0740 Soluble
62 G H -1.9180 Soluble
63 K H -2.2836 Soluble
64 S H -0.9863 Soluble
65 T H 0.0995 Aggregation-prone
66 Y H 1.1220 Aggregation-prone
67 Y H -0.1873 Soluble
68 A H 0.0000 Buried
69 D H -2.2596 Soluble
70 S H -1.6560 Soluble
71 V H 0.0000 Buried
72 K H -2.3126 Soluble
74 G H -1.6249 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.6501 Soluble
78 I H 0.0000 Buried
79 S H -0.6768 Soluble
80 R H -1.2623 Soluble
81 D H -1.8074 Soluble
82 N H -1.8241 Soluble
83 S H -1.6368 Soluble
84 K H -2.4040 Soluble
85 N H -1.8502 Soluble
86 T H -1.0970 Soluble
87 L H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.2320 Soluble
91 M H 0.0000 Buried
92 N H -1.3229 Soluble
93 S H -1.2500 Soluble
94 L H 0.0000 Buried
95 R H -2.4734 Soluble
96 A H -1.8501 Soluble
97 E H -2.3020 Soluble
98 D H 0.0000 Buried
99 T H -0.7409 Soluble
100 A H 0.0000 Buried
101 V H 0.0133 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 W H -0.2545 Soluble
108 G H -1.0144 Soluble
109 D H -1.9905 Soluble
113 E H -1.2259 Soluble
114 G H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.9727 Soluble
117 I H -0.0908 Soluble
118 W H -0.3823 Soluble
119 G H 0.0000 Buried
120 Q H -1.3706 Soluble
121 G H 0.0000 Buried
122 T H -0.1042 Soluble
123 L H -0.0327 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.8479 Soluble
128 S H -0.7851 Soluble
129 A H -0.6082 Soluble
130 S H -0.7621 Soluble
131 T H -1.0017 Soluble
132 K H -1.7297 Soluble
133 G H -1.5863 Soluble
134 P H 0.0000 Buried
135 S H -0.4598 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.8495 Soluble
139 L H 0.0000 Buried
140 A H -0.2566 Soluble
141 P H -0.2969 Soluble
142 S H -0.2951 Soluble
143 S H -0.4006 Soluble
144 T H -0.3292 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.5951 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.4089 Soluble
158 P H -0.5989 Soluble
159 V H -0.5673 Soluble
160 T H -0.4848 Soluble
161 V H -0.2061 Soluble
162 S H -0.4412 Soluble
163 W H 0.0000 Buried
164 N H -1.0122 Soluble
165 S H -0.7689 Soluble
166 G H -0.5727 Soluble
167 A H -0.3022 Soluble
168 L H -0.0987 Soluble
169 T H -0.2205 Soluble
170 S H -0.2072 Soluble
171 G H -0.2626 Soluble
172 V H 0.0957 Aggregation-prone
173 H H -0.1917 Soluble
174 T H 0.0478 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.3021 Soluble
177 A H 0.1943 Aggregation-prone
178 V H 0.3533 Aggregation-prone
179 L H 0.8107 Aggregation-prone
180 Q H 0.0681 Aggregation-prone
181 S H -0.2252 Soluble
182 S H -0.2998 Soluble
183 G H -0.3446 Soluble
184 L H -0.1480 Soluble
185 Y H 0.2458 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.2121 Soluble
193 V H 0.0000 Buried
194 P H -0.4424 Soluble
195 S H -0.5031 Soluble
196 S H -0.5308 Soluble
197 S H 0.0000 Buried
198 L H -0.6238 Soluble
199 G H -0.8131 Soluble
200 T H -0.7730 Soluble
201 Q H -1.0838 Soluble
202 T H -0.9760 Soluble
203 Y H 0.0000 Buried
204 I H -1.2814 Soluble
205 C H 0.0000 Buried
206 N H -1.5700 Soluble
207 V H 0.0000 Buried
208 N H -1.7419 Soluble
209 H H 0.0000 Buried
210 K H -2.7042 Soluble
211 P H -1.6574 Soluble
212 S H -1.9748 Soluble
213 N H -2.6012 Soluble
214 T H -2.2351 Soluble
215 K H -2.6452 Soluble
216 V H -1.6886 Soluble
217 D H -2.5620 Soluble
218 K H -2.0229 Soluble
219 K H -2.2628 Soluble
220 V H 0.0000 Buried
221 E H -1.2990 Soluble
222 P H -1.0507 Soluble
1 D L -1.7787 Soluble
2 I L -1.6375 Soluble
3 Q L -2.1967 Soluble
4 M L 0.0000 Buried
5 T L -1.3673 Soluble
6 Q L 0.0000 Buried
7 S L -0.7604 Soluble
8 P L -0.5490 Soluble
9 S L -0.6844 Soluble
10 S L -0.5861 Soluble
11 L L -0.5227 Soluble
12 S L -0.9887 Soluble
13 A L -1.1753 Soluble
14 S L -1.0916 Soluble
15 V L -0.3976 Soluble
16 G L -0.8290 Soluble
17 D L -1.9731 Soluble
18 R L -2.3446 Soluble
19 V L 0.0000 Buried
20 T L -0.6495 Soluble
21 I L 0.0000 Buried
22 T L -0.8997 Soluble
23 C L 0.0000 Buried
24 R L -2.9546 Soluble
25 A L 0.0000 Buried
26 S L -2.0802 Soluble
27 Q L -2.2216 Soluble
28 G L -1.5588 Soluble
29 I L 0.0000 Buried
36 S L -0.9074 Soluble
37 N L -0.7720 Soluble
38 W L 0.2619 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.4976 Soluble
46 P L -1.1635 Soluble
47 G L -1.5570 Soluble
48 K L -2.4917 Soluble
49 A L -1.6002 Soluble
50 P L 0.0000 Buried
51 K L -1.7585 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0442 Aggregation-prone
56 G L 0.0000 Buried
57 A L 0.0000 Buried
65 S L -0.2430 Soluble
66 S L -0.0130 Soluble
67 L L 0.3809 Aggregation-prone
68 Q L -0.3797 Soluble
69 S L -0.4286 Soluble
70 G L -0.5270 Soluble
71 V L -0.4147 Soluble
72 P L -0.4211 Soluble
74 S L -0.4521 Soluble
75 R L -0.8041 Soluble
76 F L 0.0000 Buried
77 S L -0.2208 Soluble
78 G L 0.0000 Buried
79 S L -0.7525 Soluble
80 G L -1.2173 Soluble
83 S L -1.2896 Soluble
84 G L -1.4442 Soluble
85 T L -2.1021 Soluble
86 D L -2.4264 Soluble
87 F L 0.0000 Buried
88 T L -0.7684 Soluble
89 L L 0.0000 Buried
90 T L -0.5856 Soluble
91 I L 0.0000 Buried
92 S L -1.2405 Soluble
93 S L -1.1222 Soluble
94 L L 0.0000 Buried
95 Q L -1.0848 Soluble
96 P L -1.8098 Soluble
97 E L -2.3317 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 V L -0.2876 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.5763 Aggregation-prone
108 S L 0.3837 Aggregation-prone
109 S L 0.4349 Aggregation-prone
114 F L 1.6674 Aggregation-prone
115 P L 0.6039 Aggregation-prone
116 T L 0.1827 Aggregation-prone
117 T L -0.4381 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.4936 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.8487 Soluble
124 V L 0.0000 Buried
125 E L -1.1018 Soluble
126 I L 0.0000 Buried
127 K L -1.6436 Soluble
128 R L -0.9187 Soluble
129 T L 0.2064 Aggregation-prone
130 V L 1.2338 Aggregation-prone
131 A L 0.3611 Aggregation-prone
132 A L 0.1419 Aggregation-prone
133 P L 0.0043 Aggregation-prone
134 S L -0.0797 Soluble
135 V L 0.0000 Buried
136 F L -0.0821 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.8193 Soluble
140 P L -0.9229 Soluble
141 S L -1.4606 Soluble
142 D L -2.6913 Soluble
143 E L -2.4513 Soluble
144 Q L 0.0000 Buried
145 L L -2.0804 Soluble
146 K L -2.6956 Soluble
147 S L -1.6463 Soluble
148 G L -1.3467 Soluble
149 T L -1.1426 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8356 Soluble
159 F L 0.0000 Buried
160 Y L -0.9585 Soluble
161 P L -1.6567 Soluble
162 R L -3.0857 Soluble
163 E L -3.2691 Soluble
164 A L -2.4006 Soluble
165 K L -2.7626 Soluble
166 V L 0.0000 Buried
167 Q L -0.7874 Soluble
168 W L 0.0000 Buried
169 K L -0.4908 Soluble
170 V L 0.0000 Buried
171 D L -1.5631 Soluble
172 N L -1.3103 Soluble
173 A L -0.0858 Soluble
174 L L 0.7921 Aggregation-prone
175 Q L -0.1334 Soluble
176 S L -0.3636 Soluble
177 G L -0.7835 Soluble
178 N L -0.9727 Soluble
179 S L -1.2531 Soluble
180 Q L -1.5281 Soluble
181 E L -2.1147 Soluble
182 S L -0.8078 Soluble
183 V L -0.3986 Soluble
184 T L 0.0000 Buried
185 E L -1.4650 Soluble
186 Q L 0.0000 Buried
187 D L -1.8563 Soluble
188 S L -2.1719 Soluble
189 K L -2.7395 Soluble
190 D L -2.1600 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7556 Soluble
199 L L 0.0000 Buried
200 T L -0.4696 Soluble
201 L L -0.6576 Soluble
202 S L -1.0353 Soluble
203 K L -2.1031 Soluble
204 A L -1.8706 Soluble
205 D L -2.4429 Soluble
206 Y L 0.0000 Buried
207 E L -3.8118 Soluble
208 K L -3.5272 Soluble
209 H L -3.1491 Soluble
210 K L -3.5830 Soluble
211 V L -1.7401 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9781 Soluble
216 V L 0.0000 Buried
217 T L -1.2384 Soluble
218 H L 0.0000 Buried
219 Q L -1.6338 Soluble
220 G L -0.1447 Soluble
221 L L -0.0774 Soluble
222 S L -0.4117 Soluble
223 S L -0.3902 Soluble
224 P L -0.5818 Soluble
225 V L -0.1225 Soluble
226 T L -0.5995 Soluble
227 K L -0.8873 Soluble
228 S L -0.7020 Soluble
229 F L -1.2890 Soluble
230 N L -2.5990 Soluble
231 R L -3.2825 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7004 4.3609 CSV PDB
4.5 -0.7378 4.2722 CSV PDB
5.0 -0.7807 4.1777 CSV PDB
5.5 -0.8205 4.0813 CSV PDB
6.0 -0.8471 3.9843 CSV PDB
6.5 -0.8524 3.8875 CSV PDB
7.0 -0.837 3.7916 CSV PDB
7.5 -0.8079 3.6987 CSV PDB
8.0 -0.7709 3.6142 CSV PDB
8.5 -0.7276 3.548 CSV PDB
9.0 -0.678 3.5078 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH