Amulirafusp / 6y90

A4DAB015_6y90_C_D | Source PDB: 6y90 | Chains: C, D | Done
99.12
Yes
10 Å
Yes
Avg A4D Score
-0.6918
Max Score
1.7571
Min Score
-3.7052
Total Score
-302.32
Score CDR
-0.2486
Score non-CDR
-0.7452

Calculation Parameters

Chain Sequence(s) QVQLQQPGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGRGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYYCARSTYYGGDWYFNVWGAGTTVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCQIVLSQSPAILSASPGEKVTMTCRASSSVSYIHWFQQKPGSSPKPWIYATSNLASGVPVRFSGSGSGTSYSLTISRVEAEDAATYYCQQWTSNPPTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) C (Heavy), D (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7052
Maximum Score 1.7571
Average Score -0.6918
Total Score -302.3195

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q C -1.4077 Soluble
2 V C -0.9507 Soluble
3 Q C -1.8124 Soluble
4 L C 0.0000 Buried
5 Q C -1.9154 Soluble
6 Q C -1.2280 Soluble
7 P C -1.1122 Soluble
8 G C -1.3735 Soluble
9 A C -1.4504 Soluble
11 E C -1.8423 Soluble
12 L C -0.8144 Soluble
13 V C 0.0000 Buried
14 K C -1.8040 Soluble
15 P C -1.4613 Soluble
16 G C -1.1161 Soluble
17 A C -0.9003 Soluble
18 S C -1.0759 Soluble
19 V C -1.3696 Soluble
20 K C -1.8759 Soluble
21 M C 0.0000 Buried
22 S C -0.8469 Soluble
23 C C 0.0000 Buried
24 K C -1.3638 Soluble
25 A C 0.0000 Buried
26 S C -0.9903 Soluble
27 G C -0.7325 Soluble
28 Y C -0.2348 Soluble
29 T C -0.1791 Soluble
30 F C 0.0000 Buried
35 T C -0.7394 Soluble
36 S C -0.1988 Soluble
37 Y C 0.3726 Aggregation-prone
38 N C 0.0086 Aggregation-prone
39 M C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 V C 0.0000 Buried
43 K C 0.0000 Buried
44 Q C -0.6339 Soluble
45 T C -0.9790 Soluble
46 P C -1.1196 Soluble
47 G C -1.5314 Soluble
48 R C -2.3509 Soluble
49 G C -1.4714 Soluble
50 L C 0.0000 Buried
51 E C -0.7535 Soluble
52 W C 0.0000 Buried
53 I C 0.0000 Buried
54 G C 0.0000 Buried
55 A C 0.0000 Buried
56 I C 0.0000 Buried
57 Y C -0.7905 Soluble
58 P C 0.0000 Buried
59 G C -1.4836 Soluble
62 N C -2.2730 Soluble
63 G C -2.1321 Soluble
64 D C -2.3563 Soluble
65 T C -1.0978 Soluble
66 S C -0.7472 Soluble
67 Y C -1.0429 Soluble
68 N C 0.0000 Buried
69 Q C -2.6507 Soluble
70 K C -2.6452 Soluble
71 F C -1.8180 Soluble
72 K C -2.6734 Soluble
74 G C -1.7405 Soluble
75 K C -1.2092 Soluble
76 A C 0.0000 Buried
77 T C -0.7739 Soluble
78 L C 0.0000 Buried
79 T C -0.6049 Soluble
80 A C -0.9362 Soluble
81 D C -1.5066 Soluble
82 K C -2.1101 Soluble
83 S C -1.1658 Soluble
84 S C -0.9401 Soluble
85 S C -1.0491 Soluble
86 T C -0.9382 Soluble
87 A C 0.0000 Buried
88 Y C -0.4357 Soluble
89 M C 0.0000 Buried
90 Q C -1.3579 Soluble
91 L C 0.0000 Buried
92 S C -0.8536 Soluble
93 S C -0.5993 Soluble
94 L C 0.0000 Buried
95 T C -1.2382 Soluble
96 S C -1.4694 Soluble
97 E C -1.9935 Soluble
98 D C 0.0000 Buried
99 S C -0.7256 Soluble
100 A C 0.0000 Buried
101 V C 0.0976 Aggregation-prone
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 A C 0.0000 Buried
106 R C 0.0000 Buried
107 S C 0.0000 Buried
108 T C 0.8738 Aggregation-prone
109 Y C 1.7571 Aggregation-prone
110 Y C 1.3983 Aggregation-prone
111 G C 0.0520 Aggregation-prone
112A G C 0.2063 Aggregation-prone
112 D C 0.5821 Aggregation-prone
113 W C 1.0832 Aggregation-prone
114 Y C 0.0000 Buried
115 F C 0.0000 Buried
116 N C -0.4013 Soluble
117 V C -0.5469 Soluble
118 W C 0.0000 Buried
119 G C 0.0000 Buried
120 A C -0.6001 Soluble
121 G C -0.4081 Soluble
122 T C 0.0000 Buried
123 T C -0.5550 Soluble
124 V C 0.0000 Buried
125 T C 0.0000 Buried
126 V C 0.0000 Buried
127 S C -0.5553 Soluble
128 A C -0.5355 Soluble
129 A C -0.4014 Soluble
130 S C -0.5809 Soluble
131 T C -0.5635 Soluble
132 K C -1.1915 Soluble
133 G C -1.1980 Soluble
134 P C 0.0000 Buried
135 S C -0.4056 Soluble
136 V C 0.0000 Buried
137 F C -0.6262 Soluble
138 P C -0.9676 Soluble
139 L C 0.0000 Buried
140 A C -1.0045 Soluble
141 P C -0.7153 Soluble
142 S C -0.5309 Soluble
143 S C -0.6376 Soluble
144 K C -0.7825 Soluble
145 S C -0.6093 Soluble
146 T C -0.6814 Soluble
147 S C -0.8116 Soluble
148 G C -0.9813 Soluble
149 G C -0.8926 Soluble
150 T C -0.5928 Soluble
151 A C -0.2510 Soluble
152 A C 0.0000 Buried
153 L C 0.0000 Buried
154 G C 0.0000 Buried
155 C C 0.0000 Buried
156 L C 0.0000 Buried
157 V C 0.0000 Buried
158 K C -0.4364 Soluble
159 D C -1.0371 Soluble
160 Y C 0.0000 Buried
161 F C 0.0000 Buried
162 P C 0.0000 Buried
163 E C -0.7678 Soluble
164 P C -1.1617 Soluble
165 V C -0.4739 Soluble
166 T C -0.3343 Soluble
167 V C 0.0777 Aggregation-prone
168 S C -0.2849 Soluble
169 W C 0.0000 Buried
170 N C -0.6236 Soluble
171 S C -0.6321 Soluble
172 G C -0.5231 Soluble
173 A C -0.2255 Soluble
174 L C 0.0418 Aggregation-prone
175 T C -0.0761 Soluble
176 S C -0.2488 Soluble
177 G C -0.3180 Soluble
178 V C 0.3857 Aggregation-prone
179 H C -0.2697 Soluble
180 T C -0.1873 Soluble
181 F C 0.0000 Buried
182 P C -0.4209 Soluble
183 A C 0.3095 Aggregation-prone
184 V C 0.8338 Aggregation-prone
185 L C 1.4654 Aggregation-prone
186 Q C 0.3265 Aggregation-prone
187 S C -0.0213 Soluble
188 S C -0.1426 Soluble
189 G C 0.1288 Aggregation-prone
190 L C 0.0305 Aggregation-prone
191 Y C 0.0000 Buried
192 S C 0.0672 Aggregation-prone
193 L C 0.1463 Aggregation-prone
194 S C 0.0000 Buried
195 S C 0.0000 Buried
196 V C 0.0000 Buried
197 V C 0.0000 Buried
198 T C -0.0141 Soluble
199 V C 0.0000 Buried
200 P C -0.4874 Soluble
201 S C -0.5806 Soluble
202 S C -0.6086 Soluble
203 S C -0.5319 Soluble
204 L C -0.7436 Soluble
205 G C -0.9579 Soluble
206 T C -0.6675 Soluble
207 Q C -0.9087 Soluble
208 T C -0.8738 Soluble
209 Y C 0.0000 Buried
210 I C -1.0376 Soluble
211 C C 0.0000 Buried
212 N C 0.0000 Buried
213 V C 0.0000 Buried
214 N C -1.8692 Soluble
215 H C 0.0000 Buried
216 K C -2.8760 Soluble
217 P C -1.8484 Soluble
218 S C -1.7762 Soluble
219 N C -2.5205 Soluble
220 T C -1.9531 Soluble
221 K C -2.8028 Soluble
222 V C -1.7144 Soluble
223 D C -2.6911 Soluble
224 K C -2.2160 Soluble
225 K C -1.9569 Soluble
226 V C 0.0000 Buried
227 E C -2.3991 Soluble
228 P C -1.6631 Soluble
229 K C -1.7685 Soluble
230 S C -0.8139 Soluble
231 C C -0.2486 Soluble
1 Q D -1.1642 Soluble
2 I D 0.1843 Aggregation-prone
3 V D 1.2371 Aggregation-prone
4 L D 0.0000 Buried
5 S D -0.6254 Soluble
6 Q D -1.0387 Soluble
7 S D -0.3792 Soluble
8 P D 0.1368 Aggregation-prone
9 A D 0.4362 Aggregation-prone
10 I D 1.4227 Aggregation-prone
11 L D 0.4986 Aggregation-prone
12 S D -0.5058 Soluble
13 A D 0.0000 Buried
14 S D -2.5413 Soluble
15 P D -2.8751 Soluble
16 G D -2.8474 Soluble
17 E D -3.7052 Soluble
18 K D -3.2653 Soluble
19 V D -1.5050 Soluble
20 T D -0.3365 Soluble
21 M D 0.0000 Buried
22 T D -0.7392 Soluble
23 C D 0.0000 Buried
24 R D -1.9727 Soluble
25 A D 0.0000 Buried
26 S D -0.3318 Soluble
27 S D -0.4621 Soluble
28 S D -0.6518 Soluble
29 V D 0.0000 Buried
37 S D -0.0964 Soluble
38 Y D 0.3353 Aggregation-prone
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 F D 0.0000 Buried
43 Q D 0.0000 Buried
44 Q D -1.4875 Soluble
45 K D -2.4333 Soluble
46 P D -1.6743 Soluble
47 G D -1.0902 Soluble
48 S D -1.2093 Soluble
49 S D -0.9269 Soluble
50 P D 0.0000 Buried
51 K D -1.6145 Soluble
52 P D -0.7352 Soluble
53 W D 0.0000 Buried
54 I D 0.0000 Buried
55 Y D 0.0000 Buried
56 A D -0.2573 Soluble
57 T D 0.0000 Buried
65 S D -0.8829 Soluble
66 N D -1.3168 Soluble
67 L D -0.1021 Soluble
68 A D -0.4676 Soluble
69 S D -0.4418 Soluble
70 G D -0.1599 Soluble
71 V D 0.4930 Aggregation-prone
72 P D 0.7667 Aggregation-prone
74 V D 1.4397 Aggregation-prone
75 R D -0.1620 Soluble
76 F D 0.0000 Buried
77 S D -0.1834 Soluble
78 G D -0.5384 Soluble
79 S D -0.7019 Soluble
80 G D -0.5930 Soluble
83 S D -0.6037 Soluble
84 G D -0.8322 Soluble
85 T D -0.9940 Soluble
86 S D -1.2481 Soluble
87 Y D 0.0000 Buried
88 S D -0.5986 Soluble
89 L D 0.0000 Buried
90 T D -0.7443 Soluble
91 I D 0.0000 Buried
92 S D -2.0277 Soluble
93 R D -3.4518 Soluble
94 V D 0.0000 Buried
95 E D -2.9454 Soluble
96 A D -2.3650 Soluble
97 E D -2.6116 Soluble
98 D D 0.0000 Buried
99 A D -1.8465 Soluble
100 A D -1.5980 Soluble
101 T D 0.0000 Buried
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 Q D 0.0000 Buried
106 Q D 0.0000 Buried
107 W D 0.0516 Aggregation-prone
108 T D -0.3177 Soluble
109 S D -0.9345 Soluble
114 N D -1.5344 Soluble
115 P D 0.0000 Buried
116 P D 0.0000 Buried
117 T D -0.1017 Soluble
118 F D 0.0000 Buried
119 G D 0.0000 Buried
120 G D -1.1181 Soluble
121 G D -0.4548 Soluble
122 T D 0.0000 Buried
123 K D -0.8824 Soluble
124 L D 0.0000 Buried
125 E D -1.0395 Soluble
126 I D 0.0000 Buried
127 K D -2.1084 Soluble
128 R D -2.1763 Soluble
129 T D -0.4872 Soluble
130 V D 0.7017 Aggregation-prone
131 A D 0.2997 Aggregation-prone
132 A D 0.0360 Aggregation-prone
133 P D 0.0000 Buried
134 S D 0.1648 Aggregation-prone
135 V D 0.0000 Buried
136 F D 0.5917 Aggregation-prone
137 I D 0.0000 Buried
138 F D 0.0000 Buried
139 P D 0.0000 Buried
140 P D -0.6096 Soluble
141 S D -1.4862 Soluble
142 D D -2.8860 Soluble
143 E D -3.0080 Soluble
144 Q D 0.0000 Buried
145 L D -2.0287 Soluble
146 K D -2.7584 Soluble
147 S D -1.7352 Soluble
148 G D -1.2316 Soluble
149 T D -0.8489 Soluble
150 A D 0.0000 Buried
151 S D 0.0000 Buried
152 V D 0.0000 Buried
153 V D 0.0000 Buried
154 C D 0.0000 Buried
155 L D 0.0000 Buried
156 L D 0.0000 Buried
157 N D -0.3728 Soluble
158 N D -0.4475 Soluble
159 F D 0.0000 Buried
160 Y D -0.9949 Soluble
161 P D -1.5160 Soluble
162 R D -2.7529 Soluble
163 E D -3.1948 Soluble
164 A D -2.2578 Soluble
165 K D -2.3380 Soluble
166 V D -1.3171 Soluble
167 Q D -0.8410 Soluble
168 W D 0.0000 Buried
169 K D -0.9434 Soluble
170 V D 0.0000 Buried
171 D D -2.4545 Soluble
172 N D -1.8311 Soluble
173 A D -0.7788 Soluble
174 L D 0.3897 Aggregation-prone
175 Q D -0.4460 Soluble
176 S D -0.8204 Soluble
177 G D -1.4700 Soluble
178 N D -1.7782 Soluble
179 S D -1.4843 Soluble
180 Q D -1.5256 Soluble
181 E D -1.4365 Soluble
182 S D -0.4553 Soluble
183 V D -0.4577 Soluble
184 T D -0.9750 Soluble
185 E D -2.2043 Soluble
186 Q D -1.6985 Soluble
187 D D -2.2627 Soluble
188 S D -2.6113 Soluble
189 K D -3.0732 Soluble
190 D D -2.8914 Soluble
191 S D 0.0000 Buried
192 T D -1.7502 Soluble
193 Y D -1.2811 Soluble
194 S D 0.0000 Buried
195 L D 0.0000 Buried
196 S D -0.5065 Soluble
197 S D 0.0000 Buried
198 T D -0.6139 Soluble
199 L D 0.0000 Buried
200 T D -0.4351 Soluble
201 L D -0.4887 Soluble
202 S D -1.1965 Soluble
203 K D -1.8386 Soluble
204 A D -1.8321 Soluble
205 D D -2.4589 Soluble
206 Y D 0.0000 Buried
207 E D -3.5182 Soluble
208 K D -3.4364 Soluble
209 H D -2.5176 Soluble
210 K D -2.4405 Soluble
211 V D -0.4601 Soluble
212 Y D 0.0000 Buried
213 A D -0.4341 Soluble
214 C D 0.0000 Buried
215 E D -0.5336 Soluble
216 V D 0.0000 Buried
217 T D -1.0425 Soluble
218 H D 0.0000 Buried
219 Q D -1.3086 Soluble
220 G D -0.3768 Soluble
221 L D -0.1633 Soluble
222 S D -0.4682 Soluble
223 S D -0.2966 Soluble
224 P D -0.1438 Soluble
225 V D 0.5792 Aggregation-prone
226 T D 0.1775 Aggregation-prone
227 K D -0.1289 Soluble
228 S D -0.1376 Soluble
229 F D 0.0000 Buried
230 N D -0.9394 Soluble
231 R D -1.6643 Soluble
232 G D -1.8323 Soluble
233 E D -2.1708 Soluble
234 C D -1.0883 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7493 2.547 CSV PDB
4.5 -0.7891 2.5298 CSV PDB
5.0 -0.837 2.5103 CSV PDB
5.5 -0.881 2.4901 CSV PDB
6.0 -0.908 2.4695 CSV PDB
6.5 -0.9082 2.4489 CSV PDB
7.0 -0.8819 2.4284 CSV PDB
7.5 -0.8381 2.4829 CSV PDB
8.0 -0.7857 2.6424 CSV PDB
8.5 -0.7284 2.8068 CSV PDB
9.0 -0.667 2.9721 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH