Ravagalimab / 6pe8

A4DAB219_6pe8_H_L | Source PDB: 6pe8 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7592
Max Score
1.1593
Min Score
-3.768
Total Score
-326.48
Score CDR
-0.5692
Score non-CDR
-0.7837

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVKPGGSLRLSCAASGFTFSDYGMNWVRQAPGKGLEWIAYISSGRGNIYYADTVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARSWGYFDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIVMTQSPDSLAVSLGERATINCKSSQSLLNRGNQKNYLTWFQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISLQAEDVAVYYCQNDYTYPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.768
Maximum Score 1.1593
Average Score -0.7592
Total Score -326.4766

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.7139 Soluble
2 V H -0.4817 Soluble
3 Q H -0.8006 Soluble
4 L H 0.0000 Buried
5 V H 0.6272 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5339 Soluble
8 G H -1.0352 Soluble
9 G H -0.6246 Soluble
11 G H -0.5703 Soluble
12 L H -0.1588 Soluble
13 V H -0.8654 Soluble
14 K H -2.0824 Soluble
15 P H -1.8595 Soluble
16 G H -1.5547 Soluble
17 G H -1.2303 Soluble
18 S H -1.1624 Soluble
19 L H -1.0680 Soluble
20 R H -2.1929 Soluble
21 L H 0.0000 Buried
22 S H -0.5032 Soluble
23 C H 0.0000 Buried
24 A H -0.2105 Soluble
25 A H 0.0000 Buried
26 S H -0.8092 Soluble
27 G H -0.9201 Soluble
28 F H -0.3991 Soluble
29 T H -0.5202 Soluble
30 F H 0.0000 Buried
35 S H -1.2350 Soluble
36 D H -1.3055 Soluble
37 Y H -0.2990 Soluble
38 G H 0.0000 Buried
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8452 Soluble
45 A H -1.2458 Soluble
46 P H -1.0238 Soluble
47 G H -1.4684 Soluble
48 K H -2.3492 Soluble
49 G H -1.5237 Soluble
50 L H 0.0000 Buried
51 E H -1.1248 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 A H 0.0000 Buried
55 Y H 0.7312 Aggregation-prone
56 I H 0.0000 Buried
57 S H -0.4512 Soluble
58 S H -1.1715 Soluble
59 G H -1.7734 Soluble
62 R H -2.1860 Soluble
63 G H -1.2596 Soluble
64 N H -0.4662 Soluble
65 I H 0.8944 Aggregation-prone
66 Y H 0.9909 Aggregation-prone
67 Y H 0.0054 Aggregation-prone
68 A H 0.0000 Buried
69 D H -2.2534 Soluble
70 T H -1.5755 Soluble
71 V H 0.0000 Buried
72 K H -2.3509 Soluble
74 G H -1.5997 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.7961 Soluble
78 I H 0.0000 Buried
79 S H -0.3375 Soluble
80 R H -1.0443 Soluble
81 D H -1.5771 Soluble
82 N H -2.0348 Soluble
83 A H -1.5204 Soluble
84 K H -2.3674 Soluble
85 N H -1.9259 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6695 Soluble
89 L H 0.0000 Buried
90 Q H -1.5742 Soluble
91 M H 0.0000 Buried
92 N H -1.4107 Soluble
93 S H -1.2412 Soluble
94 L H 0.0000 Buried
95 R H -2.3115 Soluble
96 A H -1.6985 Soluble
97 E H -2.0797 Soluble
98 D H 0.0000 Buried
99 T H -0.6975 Soluble
100 A H 0.0000 Buried
101 V H 0.0573 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 S H 0.3001 Aggregation-prone
108 W H 0.7893 Aggregation-prone
109 G H 0.3577 Aggregation-prone
114 Y H 0.3522 Aggregation-prone
115 F H 0.0000 Buried
116 D H -0.6694 Soluble
117 V H -0.4794 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.2512 Soluble
121 G H 0.0000 Buried
122 T H -0.2198 Soluble
123 T H -0.1296 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.9321 Soluble
128 S H -0.8717 Soluble
129 A H -0.5072 Soluble
130 S H -0.6288 Soluble
131 T H -0.6645 Soluble
132 K H -1.1525 Soluble
133 G H -1.3860 Soluble
134 P H 0.0000 Buried
135 S H -0.2671 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0567 Soluble
139 L H 0.0000 Buried
140 A H -1.1829 Soluble
141 P H 0.0000 Buried
142 S H -0.4674 Soluble
143 G H -0.6146 Soluble
144 T H -0.4173 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.2526 Soluble
154 Y H 0.0000 Buried
155 F H -0.1493 Soluble
156 P H 0.0000 Buried
157 E H -0.4193 Soluble
158 P H -0.8779 Soluble
159 V H 0.0000 Buried
160 T H -1.0254 Soluble
161 V H 0.0000 Buried
162 S H -0.5822 Soluble
163 W H 0.0000 Buried
164 N H -0.7321 Soluble
165 S H -0.6498 Soluble
166 G H -0.6056 Soluble
167 A H -0.2534 Soluble
168 L H -0.0241 Soluble
169 T H -0.1796 Soluble
170 S H -0.1755 Soluble
171 G H -0.1341 Soluble
172 V H 0.1436 Aggregation-prone
173 H H -0.0701 Soluble
174 T H -0.0249 Soluble
175 F H 0.0000 Buried
176 P H -0.3321 Soluble
177 A H 0.3464 Aggregation-prone
178 V H 0.5488 Aggregation-prone
179 L H 1.1593 Aggregation-prone
180 Q H 0.1542 Aggregation-prone
181 S H -0.1843 Soluble
182 S H -0.2012 Soluble
183 G H 0.1328 Aggregation-prone
184 L H 0.2490 Aggregation-prone
185 Y H 0.5181 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.2515 Aggregation-prone
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1753 Soluble
193 V H 0.0000 Buried
194 P H -0.4896 Soluble
195 S H -0.6002 Soluble
196 S H -0.6531 Soluble
197 S H -0.6746 Soluble
198 L H -0.8875 Soluble
199 G H -1.0000 Soluble
200 T H -0.7962 Soluble
201 Q H -1.3368 Soluble
202 T H -1.2151 Soluble
203 Y H 0.0000 Buried
204 I H -1.2547 Soluble
205 C H 0.0000 Buried
206 N H -1.6319 Soluble
207 V H 0.0000 Buried
208 N H -2.8215 Soluble
209 H H 0.0000 Buried
210 K H -3.0103 Soluble
211 P H -1.6820 Soluble
212 S H -1.8946 Soluble
213 N H -2.7400 Soluble
214 T H -2.3146 Soluble
215 K H -2.9658 Soluble
216 V H -1.6662 Soluble
217 D H -2.2798 Soluble
218 K H -1.8110 Soluble
219 K H -2.4439 Soluble
220 V H 0.0000 Buried
221 E H -2.8406 Soluble
222 P H -1.9553 Soluble
223 K H -2.5065 Soluble
224 S H -1.4847 Soluble
1 D L -1.4096 Soluble
2 I L 0.0000 Buried
3 V L 0.8117 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.5263 Soluble
6 Q L 0.0000 Buried
7 S L -1.0880 Soluble
8 P L -1.3429 Soluble
9 D L -2.4784 Soluble
10 S L -1.6944 Soluble
11 L L -0.9485 Soluble
12 A L -0.6017 Soluble
13 V L 0.0000 Buried
14 S L -1.1022 Soluble
15 L L -0.4106 Soluble
16 G L -1.1513 Soluble
17 E L -2.3171 Soluble
18 R L -2.6141 Soluble
19 A L 0.0000 Buried
20 T L -0.5865 Soluble
21 I L 0.0000 Buried
22 N L -1.0956 Soluble
23 C L 0.0000 Buried
24 K L -1.3732 Soluble
25 S L -0.6883 Soluble
26 S L -0.8108 Soluble
27 Q L -1.4385 Soluble
28 S L -0.9476 Soluble
29 L L 0.0000 Buried
30 L L -1.2147 Soluble
31 N L -2.1767 Soluble
32 R L -3.1379 Soluble
33 G L -2.4529 Soluble
34 N L -2.8006 Soluble
35 Q L -2.8953 Soluble
36 K L -1.9662 Soluble
37 N L 0.0000 Buried
38 Y L 0.1180 Aggregation-prone
39 L L 0.0000 Buried
40 T L 0.0000 Buried
41 W L 0.0000 Buried
42 F L 0.0000 Buried
43 Q L -1.3255 Soluble
44 Q L 0.0000 Buried
45 K L -2.9244 Soluble
46 P L -1.7712 Soluble
47 G L -1.7471 Soluble
48 Q L -2.2871 Soluble
49 P L -1.6511 Soluble
50 P L 0.0000 Buried
51 K L -2.1584 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1039 Soluble
56 W L 0.0949 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.5206 Soluble
66 T L -0.4644 Soluble
67 R L -1.3375 Soluble
68 E L -1.1237 Soluble
69 S L -0.9257 Soluble
70 G L -0.9354 Soluble
71 V L -1.1292 Soluble
72 P L -1.3418 Soluble
74 D L -2.2593 Soluble
75 R L -1.7152 Soluble
76 F L 0.0000 Buried
77 S L -0.8422 Soluble
78 G L 0.0000 Buried
79 S L -0.7012 Soluble
80 G L -1.1729 Soluble
83 S L -1.0419 Soluble
84 G L -1.0704 Soluble
85 T L -1.4881 Soluble
86 D L -2.2130 Soluble
87 F L 0.0000 Buried
88 T L -0.8265 Soluble
89 L L 0.0000 Buried
90 T L -0.6641 Soluble
91 I L 0.0000 Buried
93 S L -1.6397 Soluble
94 L L 0.0000 Buried
95 Q L -1.7707 Soluble
96 A L -2.1896 Soluble
97 E L -2.9794 Soluble
98 D L 0.0000 Buried
99 V L -1.9348 Soluble
100 A L 0.0000 Buried
101 V L -1.3014 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 N L 0.0000 Buried
107 D L 0.4120 Aggregation-prone
108 Y L 0.6168 Aggregation-prone
109 T L 0.3835 Aggregation-prone
114 Y L 0.6594 Aggregation-prone
115 P L -0.1697 Soluble
116 L L 0.0000 Buried
117 T L -0.0108 Soluble
118 F L 0.0515 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.5013 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -2.4168 Soluble
124 L L 0.0000 Buried
125 E L -1.2873 Soluble
126 I L 0.0000 Buried
127 K L -1.1299 Soluble
128 R L -0.6459 Soluble
129 T L 0.1789 Aggregation-prone
130 V L 0.7259 Aggregation-prone
131 A L 0.0965 Aggregation-prone
132 A L -0.0918 Soluble
133 P L 0.0000 Buried
134 S L -0.1657 Soluble
135 V L 0.0000 Buried
136 F L 0.0202 Aggregation-prone
137 I L 0.0503 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4951 Soluble
140 P L 0.0000 Buried
141 S L -1.7628 Soluble
142 D L -2.8809 Soluble
143 E L -2.8292 Soluble
144 Q L 0.0000 Buried
145 L L -2.1555 Soluble
146 K L -2.7912 Soluble
147 S L -1.6841 Soluble
148 G L -1.1046 Soluble
149 T L -0.9559 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -1.1562 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7066 Soluble
162 R L -3.1428 Soluble
163 E L -3.3007 Soluble
164 A L -2.4535 Soluble
165 K L -2.4913 Soluble
166 V L -1.3061 Soluble
167 Q L -0.6916 Soluble
168 W L 0.0000 Buried
169 K L -0.9450 Soluble
170 V L 0.0000 Buried
171 D L -1.8672 Soluble
172 N L -1.6350 Soluble
173 A L -0.5223 Soluble
174 L L 0.2225 Aggregation-prone
175 Q L -0.4959 Soluble
176 S L -0.7225 Soluble
177 G L -1.2990 Soluble
178 N L -1.6059 Soluble
179 S L -1.5853 Soluble
180 Q L -1.7905 Soluble
181 E L -1.4117 Soluble
182 S L -0.7337 Soluble
183 V L -0.7074 Soluble
184 T L -1.2405 Soluble
185 E L -2.4760 Soluble
186 Q L 0.0000 Buried
187 D L -2.5075 Soluble
188 S L -2.8959 Soluble
189 K L -3.0772 Soluble
190 D L -2.5399 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7794 Soluble
199 L L 0.0000 Buried
200 T L -0.5866 Soluble
201 L L -0.8742 Soluble
202 S L -1.3237 Soluble
203 K L -2.1320 Soluble
204 A L -2.1129 Soluble
205 D L -3.0777 Soluble
206 Y L 0.0000 Buried
207 E L -3.5922 Soluble
208 K L -3.7680 Soluble
209 H L -3.0082 Soluble
210 K L -2.9124 Soluble
211 V L -1.2750 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7380 Soluble
216 V L 0.0000 Buried
217 T L -1.2190 Soluble
218 H L 0.0000 Buried
219 Q L -1.7105 Soluble
220 G L -0.3766 Soluble
221 L L -0.2562 Soluble
222 S L -0.5026 Soluble
223 S L -0.4601 Soluble
224 P L -0.5586 Soluble
225 V L -0.0978 Soluble
226 T L -0.5805 Soluble
227 K L -0.8564 Soluble
228 S L -0.6242 Soluble
229 F L -0.9084 Soluble
230 N L -1.7737 Soluble
231 R L -2.4527 Soluble
232 G L -2.0924 Soluble
233 E L -2.6706 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7732 3.4501 CSV PDB
4.5 -0.8223 3.3944 CSV PDB
5.0 -0.8777 3.3307 CSV PDB
5.5 -0.9288 3.2636 CSV PDB
6.0 -0.9635 3.1954 CSV PDB
6.5 -0.9736 3.1271 CSV PDB
7.0 -0.9597 3.0594 CSV PDB
7.5 -0.9296 2.9936 CSV PDB
8.0 -0.8902 2.9328 CSV PDB
8.5 -0.8429 2.8813 CSV PDB
9.0 -0.7877 2.8433 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH