Azerutamig / 7t99

A4DAB030_7t99_H_L | Source PDB: 7t99 | Chains: H, L | Done
99.12
Yes
10 Å
Yes
Avg A4D Score
-0.6693
Max Score
2.2449
Min Score
-3.4015
Total Score
-288.47
Score CDR
-0.4992
Score non-CDR
-0.6901

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLCSPVTKCFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4015
Maximum Score 2.2449
Average Score -0.6693
Total Score -288.4722

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.2439 Aggregation-prone
3 Q H 0.3627 Aggregation-prone
4 L H 0.0000 Buried
5 V H 0.6379 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4805 Soluble
8 G H -0.9055 Soluble
9 G H -0.5318 Soluble
11 G H -0.3512 Soluble
12 L H -0.0374 Soluble
13 V H 0.0000 Buried
14 Q H -1.5935 Soluble
15 P H -1.4870 Soluble
16 G H -1.4505 Soluble
17 G H -1.1450 Soluble
18 S H -1.3082 Soluble
19 L H -1.1949 Soluble
20 R H -2.1699 Soluble
21 L H 0.0000 Buried
22 S H -0.4787 Soluble
23 C H 0.0000 Buried
24 A H -0.0916 Soluble
25 A H 0.0000 Buried
26 S H -0.0678 Soluble
27 G H -0.0994 Soluble
28 F H -0.6741 Soluble
29 N H -2.1114 Soluble
30 I H 0.0000 Buried
35 K H -2.3727 Soluble
36 D H -2.8438 Soluble
37 T H 0.0000 Buried
38 Y H -0.4086 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8611 Soluble
45 A H -1.2271 Soluble
46 P H -0.9801 Soluble
47 G H -1.4640 Soluble
48 K H -2.4160 Soluble
49 G H -1.7273 Soluble
50 L H 0.0000 Buried
51 E H -1.3534 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H -0.5651 Soluble
56 I H 0.0000 Buried
57 Y H -0.3959 Soluble
58 P H 0.0000 Buried
59 T H -1.1916 Soluble
62 N H -1.0986 Soluble
63 G H -0.3877 Soluble
64 Y H 0.4647 Aggregation-prone
65 T H -0.4408 Soluble
66 R H -1.9160 Soluble
67 Y H -1.7519 Soluble
68 A H 0.0000 Buried
69 D H -2.6026 Soluble
70 S H -1.6964 Soluble
71 V H 0.0000 Buried
72 K H -2.7717 Soluble
74 G H -1.7132 Soluble
75 R H -1.4682 Soluble
76 F H 0.0000 Buried
77 T H -1.1527 Soluble
78 I H 0.0000 Buried
79 S H -0.1946 Soluble
80 A H -0.7640 Soluble
81 D H -1.4084 Soluble
82 T H -1.4600 Soluble
83 S H -1.5140 Soluble
84 K H -2.2224 Soluble
85 N H -1.7700 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.3737 Soluble
91 M H 0.0000 Buried
92 N H -1.5139 Soluble
93 S H -1.2743 Soluble
94 L H 0.0000 Buried
95 R H -1.9838 Soluble
96 A H -1.4807 Soluble
97 E H -2.0583 Soluble
98 D H 0.0000 Buried
99 T H -0.5502 Soluble
100 A H 0.0000 Buried
101 V H 0.1554 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H -0.0439 Soluble
107 W H 0.0000 Buried
108 G H -0.6298 Soluble
109 G H -1.5169 Soluble
110 D H -2.2226 Soluble
111 G H -1.0236 Soluble
112 F H -0.1260 Soluble
113 Y H 0.5313 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.0240 Soluble
117 Y H 0.3359 Aggregation-prone
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.5462 Soluble
121 G H -0.7163 Soluble
122 T H -0.1517 Soluble
123 L H 0.1321 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.0187 Soluble
126 V H -0.7663 Soluble
127 S H -0.6401 Soluble
128 S H -0.6888 Soluble
129 A H -0.3697 Soluble
130 S H -0.4707 Soluble
131 T H -0.7169 Soluble
132 K H -0.9962 Soluble
133 G H -1.3388 Soluble
134 P H -0.5637 Soluble
135 S H -0.2979 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9258 Soluble
139 L H 0.0000 Buried
140 A H -1.1635 Soluble
141 P H 0.0000 Buried
142 S H -1.1461 Soluble
143 S H -1.6323 Soluble
144 K H -2.1205 Soluble
145 S H -1.3817 Soluble
146 T H -1.0691 Soluble
147 S H -0.9147 Soluble
148 G H -0.8864 Soluble
149 G H -0.8022 Soluble
150 T H -0.5281 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.4375 Soluble
160 Y H 0.0000 Buried
161 F H -0.2035 Soluble
162 P H 0.0000 Buried
163 E H -0.6013 Soluble
164 P H -0.8056 Soluble
165 V H -0.4591 Soluble
166 T H -0.5336 Soluble
167 V H -0.1617 Soluble
168 S H -0.4208 Soluble
169 W H 0.0000 Buried
170 N H -0.8281 Soluble
171 S H -0.7489 Soluble
172 G H -0.6364 Soluble
173 A H -0.2684 Soluble
174 L H -0.0470 Soluble
175 T H -0.2386 Soluble
176 S H -0.2409 Soluble
177 G H -0.2411 Soluble
178 V H 0.1198 Aggregation-prone
179 H H -0.3564 Soluble
180 T H -0.0188 Soluble
181 F H 0.0000 Buried
182 P H -0.3451 Soluble
183 A H 0.1771 Aggregation-prone
184 V H 0.4359 Aggregation-prone
185 L H 1.2928 Aggregation-prone
186 Q H 0.2236 Aggregation-prone
187 S H -0.0736 Soluble
188 S H -0.2151 Soluble
189 G H 0.0830 Aggregation-prone
190 L H 0.1516 Aggregation-prone
191 Y H 0.4098 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1102 Soluble
199 V H 0.0000 Buried
200 P H -0.5039 Soluble
201 S H -0.8118 Soluble
202 S H -0.7256 Soluble
203 S H -0.6750 Soluble
204 L H -0.9814 Soluble
205 G H -0.9567 Soluble
206 T H -0.8424 Soluble
207 Q H -1.4865 Soluble
208 T H -1.1756 Soluble
209 Y H 0.0000 Buried
210 I H -0.9990 Soluble
211 C H 0.0000 Buried
212 N H -1.5163 Soluble
213 V H 0.0000 Buried
214 N H -1.7631 Soluble
215 H H 0.0000 Buried
216 K H -2.7507 Soluble
217 P H -1.5887 Soluble
218 S H -1.7878 Soluble
219 N H -2.4961 Soluble
220 T H -2.1800 Soluble
221 K H -2.7138 Soluble
222 V H -1.7143 Soluble
223 D H -2.4694 Soluble
224 K H -1.9226 Soluble
225 K H -2.0548 Soluble
226 V H 0.0000 Buried
227 E H -2.6763 Soluble
228 P H -1.8967 Soluble
229 K H -2.4338 Soluble
1 D L -2.2664 Soluble
2 I L 0.0000 Buried
3 Q L -2.1369 Soluble
4 M L 0.0000 Buried
5 T L -1.3241 Soluble
6 Q L 0.0000 Buried
7 S L -0.7680 Soluble
8 P L -0.5960 Soluble
9 S L -0.7794 Soluble
10 S L -0.7125 Soluble
11 L L -0.5932 Soluble
12 S L -1.0182 Soluble
13 A L -1.1223 Soluble
14 S L -0.9360 Soluble
15 V L 0.2828 Aggregation-prone
16 G L -0.6394 Soluble
17 D L -1.8239 Soluble
18 R L -2.3124 Soluble
19 V L 0.0000 Buried
20 T L -0.6602 Soluble
21 I L 0.0000 Buried
22 T L -0.7524 Soluble
23 C L 0.0000 Buried
24 R L -2.6590 Soluble
25 A L 0.0000 Buried
26 S L -2.1151 Soluble
27 Q L -2.9142 Soluble
28 D L -2.6987 Soluble
29 V L 0.0000 Buried
36 N L -1.4932 Soluble
37 T L -0.5182 Soluble
38 A L 0.0801 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6923 Soluble
46 P L -1.4917 Soluble
47 G L -1.7965 Soluble
48 K L -2.5505 Soluble
49 A L -1.5890 Soluble
50 P L 0.0000 Buried
51 K L -1.1879 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.2417 Aggregation-prone
56 S L 0.6091 Aggregation-prone
57 A L 0.0000 Buried
65 S L 0.7652 Aggregation-prone
66 F L 2.2449 Aggregation-prone
67 L L 1.5289 Aggregation-prone
68 Y L 0.7583 Aggregation-prone
69 S L 0.0505 Aggregation-prone
70 G L -0.3834 Soluble
71 V L -0.1777 Soluble
72 P L -0.2951 Soluble
74 S L -0.3863 Soluble
75 R L -0.8319 Soluble
76 F L 0.0000 Buried
77 S L 0.0170 Aggregation-prone
78 G L 0.0853 Aggregation-prone
79 S L -0.5722 Soluble
80 R L -1.7172 Soluble
83 S L -1.3940 Soluble
84 G L -1.7498 Soluble
85 T L -2.1376 Soluble
86 D L -1.6777 Soluble
87 F L 0.0000 Buried
88 T L -0.7547 Soluble
89 L L 0.0000 Buried
90 T L -0.6254 Soluble
91 I L 0.0000 Buried
92 S L -1.3460 Soluble
93 S L -0.9539 Soluble
94 L L 0.0000 Buried
95 Q L -0.6631 Soluble
96 P L -1.4320 Soluble
97 E L -1.4865 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.6830 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.0000 Buried
108 Y L 0.9745 Aggregation-prone
109 T L 0.2423 Aggregation-prone
114 T L -0.4697 Soluble
115 P L -0.9652 Soluble
116 P L 0.0000 Buried
117 T L -0.7926 Soluble
118 F L 0.0000 Buried
119 G L -1.1937 Soluble
120 Q L -1.6019 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.1083 Soluble
124 V L 0.0000 Buried
125 E L -1.8137 Soluble
126 I L 0.0000 Buried
127 K L -1.6605 Soluble
128 R L -1.0752 Soluble
129 T L -0.2611 Soluble
130 V L 0.1033 Aggregation-prone
131 A L -0.1366 Soluble
132 A L -0.0264 Soluble
133 P L 0.0000 Buried
134 S L -0.1247 Soluble
135 V L 0.0000 Buried
136 F L 0.2194 Aggregation-prone
137 I L 0.2902 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4459 Soluble
140 P L -0.7087 Soluble
141 S L -1.6014 Soluble
142 D L -2.6479 Soluble
143 E L -2.3605 Soluble
144 Q L 0.0000 Buried
145 L L -1.9658 Soluble
146 K L -2.6266 Soluble
147 S L -1.6313 Soluble
148 G L -1.1692 Soluble
149 T L -0.9147 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8891 Soluble
159 F L 0.0000 Buried
160 Y L -1.1375 Soluble
161 P L -1.7154 Soluble
162 R L -2.9528 Soluble
163 E L -3.1218 Soluble
164 A L -2.1268 Soluble
165 K L -2.2505 Soluble
166 V L -1.0097 Soluble
167 Q L -0.6003 Soluble
168 W L 0.0000 Buried
169 K L -0.5001 Soluble
170 V L 0.0000 Buried
171 D L -1.9490 Soluble
172 N L -1.5605 Soluble
173 A L -0.3824 Soluble
174 L L 0.5634 Aggregation-prone
175 Q L -0.4560 Soluble
177 G L -0.9601 Soluble
178 N L -0.9005 Soluble
179 S L -1.3996 Soluble
180 Q L -1.8781 Soluble
181 E L -2.3486 Soluble
182 S L 0.0000 Buried
183 V L -0.8148 Soluble
184 T L -1.1294 Soluble
185 E L -2.3201 Soluble
186 Q L 0.0000 Buried
187 D L -1.9262 Soluble
188 S L -1.9371 Soluble
189 K L -2.3756 Soluble
190 D L -1.6904 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8231 Soluble
199 L L 0.0000 Buried
200 T L -0.3596 Soluble
201 L L -0.5482 Soluble
202 S L -0.9840 Soluble
203 K L -2.0390 Soluble
204 A L -1.7357 Soluble
205 D L -2.3761 Soluble
206 Y L 0.0000 Buried
207 E L -3.0986 Soluble
208 K L -3.4015 Soluble
209 H L -2.7435 Soluble
210 K L -2.7884 Soluble
211 V L -1.1705 Soluble
212 Y L 0.0000 Buried
213 A L -0.3882 Soluble
214 C L 0.0000 Buried
215 E L -0.4839 Soluble
216 V L 0.0000 Buried
217 T L -0.9598 Soluble
218 H L 0.0000 Buried
219 Q L -1.3434 Soluble
220 G L -0.1931 Soluble
221 L L 0.2177 Aggregation-prone
222 C L 0.5054 Aggregation-prone
223 S L 0.0630 Aggregation-prone
224 P L -0.1637 Soluble
225 V L 0.2159 Aggregation-prone
226 T L -0.1967 Soluble
227 K L -0.2192 Soluble
228 C L 0.2679 Aggregation-prone
229 F L -0.4184 Soluble
230 N L -1.7386 Soluble
231 R L -2.0014 Soluble
232 G L -1.2264 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6659 4.0723 CSV PDB
4.5 -0.7088 4.0723 CSV PDB
5.0 -0.7561 4.0723 CSV PDB
5.5 -0.7989 4.0723 CSV PDB
6.0 -0.827 4.0723 CSV PDB
6.5 -0.8329 4.0723 CSV PDB
7.0 -0.8178 4.0723 CSV PDB
7.5 -0.7895 4.0723 CSV PDB
8.0 -0.7536 4.0722 CSV PDB
8.5 -0.711 4.0721 CSV PDB
9.0 -0.661 4.0716 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH