Caxmotabart / 8tjf

Calculation Parameters

Chain Sequence(s)	`EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVCPLAPSGTADLGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKGQPKAAPSVRLFPPCSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE` input.pdb
Selected Chain(s)	H (Heavy), L (Light)
Distance of Aggregation	10 Å
FoldX Energy Minimization	Yes
pH Calculations	Yes
Downloads	Download all data (.tar.gz)

Aggrescan4D Profile

Chain: Show buried residues (score = 0)

Aggregation-prone (score > 0)

Soluble (score < 0)

Buried (score = 0)

Per-Residue A4D Scores

Download CSV

Minimum Score -3.1952

Maximum Score 2.0841

Average Score -0.725

Total Score -308.8552

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Classification:

Index	Residue	Chain	A4D Score	Classification
1	E	H	-2.1025	Soluble
2	V	H	-1.3344	Soluble
3	Q	H	-1.1081	Soluble
4	L	H	0.0000	Buried
5	V	H	0.7146	Aggregation-prone
6	E	H	0.0000	Buried
7	S	H	-0.4125	Soluble
8	G	H	-0.8527	Soluble
9	G	H	-0.0969	Soluble
11	G	H	0.6049	Aggregation-prone
12	L	H	0.8495	Aggregation-prone
13	V	H	-0.2884	Soluble
14	Q	H	-1.4578	Soluble
15	P	H	-1.6976	Soluble
16	G	H	-1.4047	Soluble
17	G	H	-1.0978	Soluble
18	S	H	-1.0564	Soluble
19	L	H	-1.0507	Soluble
20	R	H	-2.1253	Soluble
21	L	H	0.0000	Buried
22	S	H	-0.4288	Soluble
23	C	H	0.0000	Buried
24	A	H	-0.2135	Soluble
25	A	H	0.0000	Buried
26	S	H	-1.0769	Soluble
27	G	H	-1.4941	Soluble
28	F	H	-1.5987	Soluble
29	N	H	-2.3538	Soluble
30	I	H	0.0000	Buried
35	K	H	-2.9809	Soluble
36	D	H	-3.1222	Soluble
37	T	H	0.0000	Buried
38	Y	H	-0.2491	Soluble
39	I	H	0.0000	Buried
40	H	H	0.0000	Buried
41	W	H	0.0000	Buried
42	V	H	0.0000	Buried
43	R	H	0.0000	Buried
44	Q	H	-0.7295	Soluble
45	A	H	-1.1079	Soluble
46	P	H	-0.8381	Soluble
47	G	H	-1.4599	Soluble
48	K	H	-2.4435	Soluble
49	G	H	-1.7024	Soluble
50	L	H	0.0000	Buried
51	E	H	-1.3344	Soluble
52	W	H	0.0000	Buried
53	V	H	0.0000	Buried
54	A	H	0.0000	Buried
55	R	H	-0.5122	Soluble
56	I	H	0.0000	Buried
57	Y	H	-0.4685	Soluble
58	P	H	-1.2141	Soluble
59	T	H	-1.3141	Soluble
62	N	H	-0.9590	Soluble
63	G	H	-0.5102	Soluble
64	Y	H	0.5437	Aggregation-prone
65	T	H	-0.4040	Soluble
66	R	H	-1.9057	Soluble
67	Y	H	-1.6486	Soluble
68	A	H	-1.7456	Soluble
69	D	H	-2.6140	Soluble
70	S	H	-1.6867	Soluble
71	V	H	0.0000	Buried
72	K	H	-2.7126	Soluble
74	G	H	-1.6256	Soluble
75	R	H	0.0000	Buried
76	F	H	0.0000	Buried
77	T	H	-1.0199	Soluble
78	I	H	0.0000	Buried
79	S	H	-0.1306	Soluble
80	A	H	-0.8119	Soluble
81	D	H	-1.5133	Soluble
82	T	H	-1.6324	Soluble
83	S	H	-1.3911	Soluble
84	K	H	-2.2091	Soluble
85	N	H	-2.0052	Soluble
86	T	H	0.0000	Buried
87	A	H	0.0000	Buried
88	Y	H	-0.5763	Soluble
89	L	H	0.0000	Buried
90	Q	H	-1.2413	Soluble
91	M	H	0.0000	Buried
92	N	H	-1.3530	Soluble
93	S	H	-1.2052	Soluble
94	L	H	0.0000	Buried
95	R	H	-2.4089	Soluble
96	A	H	-1.7921	Soluble
97	E	H	-2.2706	Soluble
98	D	H	0.0000	Buried
99	T	H	-0.5277	Soluble
100	A	H	0.0000	Buried
101	V	H	0.5625	Aggregation-prone
102	Y	H	0.0000	Buried
103	Y	H	0.0000	Buried
104	C	H	0.0000	Buried
105	S	H	0.0000	Buried
106	R	H	-0.1732	Soluble
107	W	H	0.0000	Buried
108	G	H	-0.5760	Soluble
109	G	H	-1.5068	Soluble
110	D	H	-2.1324	Soluble
111	G	H	-0.9969	Soluble
112	F	H	-0.1136	Soluble
113	Y	H	0.4726	Aggregation-prone
114	A	H	0.0000	Buried
115	M	H	0.0000	Buried
116	D	H	-0.1039	Soluble
117	Y	H	-0.1553	Soluble
118	W	H	-0.4004	Soluble
119	G	H	0.0000	Buried
120	Q	H	-1.4223	Soluble
121	G	H	-0.4404	Soluble
122	T	H	0.4197	Aggregation-prone
123	L	H	1.4416	Aggregation-prone
124	V	H	0.0000	Buried
125	T	H	0.1148	Aggregation-prone
126	V	H	0.0000	Buried
127	S	H	-0.8900	Soluble
128	S	H	-0.8406	Soluble
129	A	H	-0.5539	Soluble
130	S	H	-0.6572	Soluble
131	T	H	-0.7044	Soluble
132	K	H	-1.1839	Soluble
133	G	H	-1.3935	Soluble
134	P	H	0.0000	Buried
135	S	H	-0.6114	Soluble
136	V	H	0.0000	Buried
137	C	H	0.0000	Buried
138	P	H	-1.1810	Soluble
139	L	H	0.0000	Buried
140	A	H	-1.2417	Soluble
141	P	H	0.0000	Buried
142	S	H	-0.7869	Soluble
143	G	H	-0.6316	Soluble
144	T	H	-0.5042	Soluble
145	A	H	-0.3927	Soluble
146	D	H	-0.4062	Soluble
147	L	H	0.0000	Buried
148	G	H	0.0000	Buried
149	C	H	0.0000	Buried
150	L	H	0.0000	Buried
151	V	H	0.0000	Buried
152	K	H	-0.4900	Soluble
153	D	H	-0.6233	Soluble
154	Y	H	0.0000	Buried
155	F	H	0.0000	Buried
156	P	H	-0.6403	Soluble
157	E	H	-0.9770	Soluble
158	P	H	-0.9885	Soluble
159	V	H	-0.6993	Soluble
160	T	H	-0.5142	Soluble
161	V	H	-0.2920	Soluble
162	S	H	-0.4008	Soluble
163	W	H	0.0000	Buried
164	N	H	-0.8745	Soluble
165	S	H	-0.6750	Soluble
166	G	H	-0.5337	Soluble
167	A	H	-0.2522	Soluble
168	L	H	0.0306	Aggregation-prone
169	T	H	-0.1292	Soluble
170	S	H	-0.1469	Soluble
171	G	H	-0.1659	Soluble
172	V	H	0.0820	Aggregation-prone
173	H	H	-0.4049	Soluble
174	T	H	-0.1938	Soluble
175	F	H	0.0000	Buried
176	P	H	-0.2583	Soluble
177	A	H	0.1868	Aggregation-prone
178	V	H	0.0000	Buried
179	L	H	1.1091	Aggregation-prone
180	Q	H	0.2659	Aggregation-prone
181	S	H	-0.1139	Soluble
182	S	H	-0.1883	Soluble
183	G	H	0.0345	Aggregation-prone
184	L	H	0.0076	Aggregation-prone
185	Y	H	0.2102	Aggregation-prone
186	S	H	0.0000	Buried
187	L	H	0.0000	Buried
188	S	H	0.0000	Buried
189	S	H	0.0000	Buried
190	V	H	0.0000	Buried
191	V	H	0.0000	Buried
192	T	H	-0.2711	Soluble
193	V	H	0.0000	Buried
194	P	H	-0.6205	Soluble
195	S	H	-0.4838	Soluble
196	S	H	-0.5576	Soluble
197	S	H	-0.5793	Soluble
198	L	H	-0.8729	Soluble
199	G	H	-1.3514	Soluble
200	T	H	-1.0121	Soluble
201	Q	H	-1.4438	Soluble
202	T	H	-1.2732	Soluble
203	Y	H	0.0000	Buried
204	I	H	-1.6027	Soluble
205	C	H	0.0000	Buried
206	N	H	0.0000	Buried
207	V	H	0.0000	Buried
208	N	H	-1.7592	Soluble
209	H	H	0.0000	Buried
210	K	H	-2.6640	Soluble
211	P	H	-1.6552	Soluble
212	S	H	-1.7750	Soluble
213	N	H	-2.4943	Soluble
214	T	H	-2.0210	Soluble
215	K	H	-2.5715	Soluble
216	V	H	-1.5511	Soluble
217	D	H	-2.6451	Soluble
218	K	H	-2.2991	Soluble
219	R	H	-2.8413	Soluble
220	V	H	0.0000	Buried
221	E	H	-2.9219	Soluble
222	P	H	-1.8567	Soluble
223	K	H	-2.2265	Soluble
1	D	L	-2.2382	Soluble
2	I	L	0.0000	Buried
3	Q	L	-2.2206	Soluble
4	M	L	0.0000	Buried
5	T	L	-1.3728	Soluble
6	Q	L	0.0000	Buried
7	S	L	-0.8991	Soluble
8	P	L	-0.6964	Soluble
9	S	L	-0.9617	Soluble
10	S	L	-1.0565	Soluble
11	L	L	-0.4990	Soluble
12	S	L	-0.7235	Soluble
13	A	L	0.0000	Buried
14	S	L	-0.1569	Soluble
15	V	L	0.6586	Aggregation-prone
16	G	L	-0.6337	Soluble
17	D	L	-1.5536	Soluble
18	R	L	-2.2382	Soluble
19	V	L	0.0000	Buried
20	T	L	-0.6571	Soluble
21	I	L	0.0000	Buried
22	T	L	-0.7557	Soluble
23	C	L	0.0000	Buried
24	R	L	-2.6071	Soluble
25	A	L	0.0000	Buried
26	S	L	-2.1571	Soluble
27	Q	L	-2.7409	Soluble
28	D	L	-2.8037	Soluble
29	V	L	0.0000	Buried
36	N	L	-1.8821	Soluble
37	T	L	-1.0050	Soluble
38	A	L	-0.0600	Soluble
39	V	L	0.0000	Buried
40	A	L	0.0000	Buried
41	W	L	0.0000	Buried
42	Y	L	0.0000	Buried
43	Q	L	0.0000	Buried
44	Q	L	0.0000	Buried
45	K	L	-1.7613	Soluble
46	P	L	-1.2404	Soluble
47	G	L	-1.6723	Soluble
48	K	L	-2.6163	Soluble
49	A	L	-1.6420	Soluble
50	P	L	0.0000	Buried
51	K	L	-1.4910	Soluble
52	L	L	0.0000	Buried
53	L	L	0.0000	Buried
54	I	L	0.0000	Buried
55	Y	L	1.0161	Aggregation-prone
56	S	L	0.6308	Aggregation-prone
57	A	L	0.0000	Buried
65	S	L	0.6317	Aggregation-prone
66	F	L	2.0841	Aggregation-prone
67	L	L	1.3175	Aggregation-prone
68	Y	L	0.5440	Aggregation-prone
69	S	L	-0.0025	Soluble
70	G	L	-0.4852	Soluble
71	V	L	0.0000	Buried
72	P	L	-0.3543	Soluble
74	S	L	-0.3993	Soluble
75	R	L	-0.8053	Soluble
76	F	L	0.0000	Buried
77	S	L	0.0463	Aggregation-prone
78	G	L	0.0000	Buried
79	S	L	-0.6431	Soluble
80	R	L	-1.7329	Soluble
83	S	L	-1.2687	Soluble
84	G	L	-1.8444	Soluble
85	T	L	-2.2207	Soluble
86	D	L	-1.9104	Soluble
87	F	L	0.0000	Buried
88	T	L	-0.7825	Soluble
89	L	L	0.0000	Buried
90	T	L	-0.5974	Soluble
91	I	L	0.0000	Buried
92	S	L	-1.3081	Soluble
93	S	L	-1.0708	Soluble
94	L	L	0.0000	Buried
95	Q	L	-0.8939	Soluble
96	P	L	-0.8111	Soluble
97	E	L	-1.9027	Soluble
98	D	L	0.0000	Buried
99	F	L	-0.5639	Soluble
100	A	L	-0.9385	Soluble
101	T	L	0.0000	Buried
102	Y	L	0.0000	Buried
103	Y	L	0.0000	Buried
104	C	L	0.0000	Buried
105	Q	L	0.0000	Buried
106	Q	L	0.0000	Buried
107	H	L	0.5744	Aggregation-prone
108	Y	L	0.7751	Aggregation-prone
109	T	L	0.2771	Aggregation-prone
114	T	L	-0.3781	Soluble
115	P	L	-0.8498	Soluble
116	P	L	0.0000	Buried
117	T	L	-0.7017	Soluble
118	F	L	-0.5884	Soluble
119	G	L	0.0000	Buried
120	Q	L	-2.0169	Soluble
121	G	L	0.0000	Buried
122	T	L	0.0000	Buried
123	K	L	-1.9880	Soluble
124	V	L	0.0000	Buried
125	E	L	-1.1246	Soluble
126	I	L	0.3488	Aggregation-prone
127	K	L	-1.2519	Soluble
128	G	L	-1.5819	Soluble
129	Q	L	-1.9299	Soluble
130	P	L	-1.8720	Soluble
131	K	L	-2.4981	Soluble
132	A	L	-1.5070	Soluble
133	A	L	-0.9408	Soluble
134	P	L	0.0000	Buried
135	S	L	-0.3083	Soluble
136	V	L	0.0000	Buried
137	R	L	-0.5242	Soluble
138	L	L	-0.4283	Soluble
139	F	L	0.0000	Buried
140	P	L	-0.0778	Soluble
141	P	L	-0.4456	Soluble
142	C	L	0.0000	Buried
143	S	L	-1.1183	Soluble
144	E	L	-1.6816	Soluble
145	E	L	-1.8238	Soluble
146	L	L	-1.8032	Soluble
147	Q	L	-2.0724	Soluble
148	A	L	-1.8209	Soluble
149	N	L	-2.7389	Soluble
150	K	L	-2.8593	Soluble
151	A	L	0.0000	Buried
152	T	L	0.0000	Buried
153	L	L	0.0000	Buried
154	V	L	0.0000	Buried
155	C	L	0.0000	Buried
156	L	L	0.0000	Buried
157	I	L	0.0000	Buried
158	S	L	0.0000	Buried
159	D	L	-1.6752	Soluble
160	F	L	0.0000	Buried
161	Y	L	-1.6881	Soluble
162	P	L	-1.4531	Soluble
163	G	L	-1.0278	Soluble
164	A	L	-0.1010	Soluble
165	V	L	0.1002	Aggregation-prone
166	T	L	0.0216	Aggregation-prone
167	V	L	0.2686	Aggregation-prone
168	A	L	-0.3475	Soluble
169	W	L	0.0000	Buried
170	K	L	-1.0657	Soluble
171	A	L	0.0000	Buried
172	D	L	-1.3350	Soluble
173	S	L	-0.9108	Soluble
174	S	L	-0.7888	Soluble
175	P	L	-0.8728	Soluble
176	V	L	-0.8557	Soluble
177	K	L	-1.6826	Soluble
178	A	L	-0.8599	Soluble
179	G	L	-0.7419	Soluble
180	V	L	-0.4100	Soluble
181	E	L	-0.3561	Soluble
182	T	L	0.0349	Aggregation-prone
183	T	L	0.0343	Aggregation-prone
184	T	L	-0.1573	Soluble
185	P	L	-0.5999	Soluble
186	S	L	-1.3546	Soluble
187	K	L	-2.8256	Soluble
188	Q	L	-2.4326	Soluble
189	S	L	-1.8547	Soluble
190	N	L	-2.5342	Soluble
191	N	L	-2.9355	Soluble
192	K	L	-2.7141	Soluble
193	Y	L	-1.7875	Soluble
194	A	L	0.0000	Buried
195	A	L	0.0000	Buried
196	S	L	0.0000	Buried
197	S	L	0.0000	Buried
198	Y	L	0.0000	Buried
199	L	L	0.0000	Buried
200	S	L	-0.6980	Soluble
201	L	L	0.0000	Buried
202	T	L	-2.0383	Soluble
203	P	L	-2.5492	Soluble
204	E	L	-3.1952	Soluble
205	Q	L	-2.3514	Soluble
206	W	L	0.0000	Buried
207	K	L	-3.1225	Soluble
208	S	L	-2.4081	Soluble
209	H	L	-2.3883	Soluble
210	R	L	-2.5153	Soluble
211	S	L	-1.4864	Soluble
212	Y	L	0.0000	Buried
213	S	L	0.0000	Buried
214	C	L	0.0000	Buried
215	Q	L	-0.8470	Soluble
216	V	L	0.0000	Buried
217	T	L	-0.5728	Soluble
218	H	L	0.0000	Buried
219	E	L	-2.3113	Soluble
220	G	L	-1.4192	Soluble
221	S	L	-0.9473	Soluble
222	T	L	-0.7398	Soluble
223	V	L	-0.5560	Soluble
224	E	L	-1.7471	Soluble
225	K	L	-1.5321	Soluble
226	T	L	-0.8740	Soluble
227	V	L	-0.5793	Soluble
228	A	L	-1.2947	Soluble
229	P	L	-1.5187	Soluble
230	T	L	-1.6039	Soluble
231	E	L	-1.8547	Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer | colored by A4D score

Load structure to view sequence…

Downloads

Download PDB View in RCSB

Color Legend (B-factor = A4D Score)

Score > 1.5 — highly aggregation-prone

Score 0.5–1.5 — aggregation-prone

Score ≈ 0 — buried

Score −1 to −0.5 — soluble

Score < −2.5 — highly soluble

pH Structure

Load structure at specific pH condition

A4D Score Threshold

Highlight residues > threshold in the sequence panel

Threshold: 0.0

CDR Regions

Outline CDR regions in sequence panel

HCDR1

HCDR2

HCDR3

LCDR1

LCDR2

LCDR3

Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH	Avg A4D Score	Max A4D Score	Downloads
4.0	-0.7375	3.5373	CSV PDB
4.5	-0.7779	3.5373	CSV PDB
5.0	-0.8242	3.5373	CSV PDB
5.5	-0.8668	3.5373	CSV PDB
6.0	-0.8944	3.5373	CSV PDB
6.5	-0.898	3.5373	CSV PDB
7.0	-0.8783	3.5373	CSV PDB
7.5	-0.8437	3.5373	CSV PDB
8.0	-0.8012	3.5372	CSV PDB
8.5	-0.7532	3.5371	CSV PDB
9.0	-0.6998	3.5369	CSV PDB