Nesfrotamig / 7t97

A4DAB178_7t97_E_F | Source PDB: 7t97 | Chains: E, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.687
Max Score
2.2098
Min Score
-3.4695
Total Score
-296.1
Score CDR
-0.5446
Score non-CDR
-0.7044

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSACTKCPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) E (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4695
Maximum Score 2.2098
Average Score -0.687
Total Score -296.0994

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V E 0.5444 Aggregation-prone
3 Q E -0.4703 Soluble
4 L E 0.0000 Buried
5 V E 0.0783 Aggregation-prone
6 E E 0.0000 Buried
7 S E -0.5279 Soluble
8 G E -0.8673 Soluble
9 G E -0.3261 Soluble
11 G E -0.3234 Soluble
12 L E 0.1038 Aggregation-prone
13 V E 0.0000 Buried
14 Q E -1.5926 Soluble
15 P E -1.8179 Soluble
16 G E -1.5043 Soluble
17 G E -1.1486 Soluble
18 S E -1.3670 Soluble
19 L E -1.1203 Soluble
20 R E -2.2418 Soluble
21 L E 0.0000 Buried
22 S E -0.5428 Soluble
23 C E 0.0000 Buried
24 A E -0.3801 Soluble
25 A E 0.0000 Buried
26 S E -0.6193 Soluble
27 G E -0.4084 Soluble
28 F E -0.9571 Soluble
29 N E -2.3980 Soluble
30 I E 0.0000 Buried
35 K E -2.9657 Soluble
36 D E -3.0754 Soluble
37 T E 0.0000 Buried
38 Y E -0.2283 Soluble
39 I E 0.0000 Buried
40 H E 0.0000 Buried
41 W E 0.0000 Buried
42 V E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.8195 Soluble
45 A E -1.2102 Soluble
46 P E -0.9673 Soluble
47 G E -1.4705 Soluble
48 K E -2.3943 Soluble
49 G E -1.6467 Soluble
50 L E 0.0000 Buried
51 E E -1.0915 Soluble
52 W E 0.0000 Buried
53 V E 0.0000 Buried
54 A E 0.0000 Buried
55 R E -0.5097 Soluble
56 I E 0.0000 Buried
57 Y E -0.4780 Soluble
58 P E 0.0000 Buried
59 T E -1.2074 Soluble
62 N E -0.9452 Soluble
63 G E -0.5950 Soluble
64 Y E 0.5090 Aggregation-prone
65 T E -0.3698 Soluble
66 R E -1.8060 Soluble
67 Y E -1.6935 Soluble
68 A E -1.7771 Soluble
69 D E -2.6278 Soluble
70 S E -1.7692 Soluble
71 V E 0.0000 Buried
72 K E -2.8074 Soluble
74 G E -1.7236 Soluble
75 R E -1.3285 Soluble
76 F E 0.0000 Buried
77 T E -1.1842 Soluble
78 I E 0.0000 Buried
79 S E -0.2225 Soluble
80 A E -0.8459 Soluble
81 D E -1.5191 Soluble
82 T E -1.6647 Soluble
83 S E -1.6236 Soluble
84 K E -2.2636 Soluble
85 N E -1.8719 Soluble
86 T E 0.0000 Buried
87 A E 0.0000 Buried
88 Y E -0.6470 Soluble
89 L E 0.0000 Buried
90 Q E -1.6339 Soluble
91 M E 0.0000 Buried
92 N E -1.4946 Soluble
93 S E -1.2971 Soluble
94 L E 0.0000 Buried
95 R E -2.4717 Soluble
96 A E -1.7957 Soluble
97 E E -2.2851 Soluble
98 D E 0.0000 Buried
99 T E -0.7147 Soluble
100 A E 0.0000 Buried
101 V E 0.1486 Aggregation-prone
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 S E 0.0000 Buried
106 R E -0.0704 Soluble
107 W E 0.0000 Buried
108 G E -0.7191 Soluble
109 G E -1.9618 Soluble
110 D E -2.2383 Soluble
111 G E -1.1029 Soluble
112 F E -0.2881 Soluble
113 Y E 0.6068 Aggregation-prone
114 A E 0.0000 Buried
115 M E 0.0000 Buried
116 D E 0.0826 Aggregation-prone
117 Y E 0.0744 Aggregation-prone
118 W E -0.3817 Soluble
119 G E 0.0000 Buried
120 Q E -1.5069 Soluble
121 G E -0.6884 Soluble
122 T E -0.0113 Soluble
123 L E 0.3516 Aggregation-prone
124 V E 0.0000 Buried
125 T E -0.4198 Soluble
126 V E 0.0000 Buried
127 S E -0.8537 Soluble
128 S E -0.5200 Soluble
129 A E -0.0810 Soluble
130 C E 0.2649 Aggregation-prone
131 T E -0.1704 Soluble
132 K E -0.9013 Soluble
133 C E -0.8338 Soluble
134 P E 0.0000 Buried
135 S E -0.2118 Soluble
136 V E 0.0000 Buried
137 F E 0.0000 Buried
138 P E -1.0039 Soluble
139 L E 0.0000 Buried
140 A E -1.3089 Soluble
141 P E 0.0000 Buried
142 S E -1.2761 Soluble
143 S E -1.0356 Soluble
144 K E -1.3399 Soluble
145 S E 0.0000 Buried
146 T E -0.7600 Soluble
147 S E -0.7423 Soluble
148 G E -0.8002 Soluble
149 G E -0.8774 Soluble
150 T E -0.5801 Soluble
151 A E 0.0000 Buried
152 A E 0.0000 Buried
153 L E 0.0000 Buried
154 G E 0.0000 Buried
155 C E 0.0000 Buried
156 L E 0.0000 Buried
157 V E 0.0000 Buried
158 K E 0.0000 Buried
159 D E -0.4557 Soluble
160 Y E 0.0000 Buried
161 F E 0.0000 Buried
162 P E 0.0000 Buried
163 E E -0.4737 Soluble
164 P E -0.6369 Soluble
165 V E -0.5699 Soluble
166 T E -0.5480 Soluble
167 V E -0.3361 Soluble
168 S E -0.3989 Soluble
169 W E 0.0000 Buried
170 N E -0.8219 Soluble
171 S E -0.7146 Soluble
172 G E -0.5979 Soluble
173 A E -0.2722 Soluble
174 L E -0.0245 Soluble
175 T E -0.1861 Soluble
176 S E -0.1789 Soluble
177 G E -0.2242 Soluble
178 V E 0.1625 Aggregation-prone
179 H E -0.2724 Soluble
180 T E -0.0983 Soluble
181 F E 0.0000 Buried
182 P E -0.2851 Soluble
183 A E 0.2575 Aggregation-prone
184 V E 0.6389 Aggregation-prone
185 L E 1.1366 Aggregation-prone
186 Q E 0.2509 Aggregation-prone
187 S E -0.1585 Soluble
188 S E -0.2213 Soluble
189 G E 0.0527 Aggregation-prone
190 L E 0.1661 Aggregation-prone
191 Y E 0.3699 Aggregation-prone
192 S E 0.2120 Aggregation-prone
193 L E 0.0000 Buried
194 S E 0.0000 Buried
195 S E 0.0000 Buried
196 V E 0.0000 Buried
197 V E 0.0000 Buried
198 T E -0.1293 Soluble
199 V E 0.0000 Buried
200 P E -0.6283 Soluble
201 S E -0.5985 Soluble
202 S E -0.6102 Soluble
203 S E 0.0000 Buried
204 L E -0.8465 Soluble
205 G E -1.0780 Soluble
206 T E -0.8414 Soluble
207 Q E -1.4644 Soluble
208 T E -1.2107 Soluble
209 Y E 0.0000 Buried
210 I E -1.2428 Soluble
211 C E 0.0000 Buried
212 N E -1.4776 Soluble
213 V E 0.0000 Buried
214 N E -1.7938 Soluble
215 H E 0.0000 Buried
216 K E -2.6543 Soluble
217 P E -1.4777 Soluble
218 S E -1.5710 Soluble
219 N E -2.3582 Soluble
220 T E -1.9393 Soluble
221 K E -2.6282 Soluble
222 V E -1.6591 Soluble
223 D E -2.5365 Soluble
224 K E -2.1259 Soluble
225 K E -2.3370 Soluble
226 V E 0.0000 Buried
227 E E -2.8013 Soluble
228 P E -2.0203 Soluble
229 K E -2.2284 Soluble
1 D F -2.2432 Soluble
2 I F 0.0000 Buried
3 Q F -2.3003 Soluble
4 M F 0.0000 Buried
5 T F -1.4237 Soluble
6 Q F 0.0000 Buried
7 S F -0.9773 Soluble
8 P F -0.7675 Soluble
9 S F -1.0536 Soluble
10 S F -1.0103 Soluble
11 L F -0.6614 Soluble
12 S F -0.7656 Soluble
13 A F -0.9059 Soluble
14 S F -0.8851 Soluble
15 V F -0.1934 Soluble
16 G F -0.8823 Soluble
17 D F -1.8629 Soluble
18 R F -2.3499 Soluble
19 V F 0.0000 Buried
20 T F -0.6148 Soluble
21 I F 0.0000 Buried
22 T F -0.7440 Soluble
23 C F 0.0000 Buried
24 R F -2.7742 Soluble
25 A F 0.0000 Buried
26 S F -2.1540 Soluble
27 Q F -2.6366 Soluble
28 D F -2.7438 Soluble
29 V F 0.0000 Buried
36 N F -1.8411 Soluble
37 T F -0.7051 Soluble
38 A F -0.0363 Soluble
39 V F 0.0000 Buried
40 A F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F 0.0000 Buried
45 K F -1.7227 Soluble
46 P F -1.3471 Soluble
47 G F -1.7287 Soluble
48 K F -2.5249 Soluble
49 A F -1.6004 Soluble
50 P F 0.0000 Buried
51 K F -1.5627 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F 1.2419 Aggregation-prone
56 S F 0.5776 Aggregation-prone
57 A F 0.0000 Buried
65 S F 0.7022 Aggregation-prone
66 F F 2.2098 Aggregation-prone
67 L F 1.4845 Aggregation-prone
68 Y F 0.6182 Aggregation-prone
69 S F 0.0003 Aggregation-prone
70 G F -0.4768 Soluble
71 V F 0.0000 Buried
72 P F -0.3514 Soluble
74 S F -0.4062 Soluble
75 R F -0.7341 Soluble
76 F F 0.0000 Buried
77 S F 0.0796 Aggregation-prone
78 G F 0.1131 Aggregation-prone
79 S F -0.5581 Soluble
80 R F -1.5055 Soluble
83 S F -1.2140 Soluble
84 G F -1.7099 Soluble
85 T F -2.1250 Soluble
86 D F -1.8149 Soluble
87 F F 0.0000 Buried
88 T F -0.7358 Soluble
89 L F 0.0000 Buried
90 T F -0.6376 Soluble
91 I F 0.0000 Buried
92 S F -1.4703 Soluble
93 S F -1.1930 Soluble
94 L F 0.0000 Buried
95 Q F -0.9484 Soluble
96 P F -1.6748 Soluble
97 E F -2.0869 Soluble
98 D F 0.0000 Buried
99 F F 0.0000 Buried
100 A F 0.0000 Buried
101 T F -1.0197 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 H F 0.5582 Aggregation-prone
108 Y F 0.8491 Aggregation-prone
109 T F 0.2834 Aggregation-prone
114 T F -0.3778 Soluble
115 P F -0.8253 Soluble
116 P F 0.0000 Buried
117 T F -0.6645 Soluble
118 F F -0.5125 Soluble
119 G F 0.0000 Buried
120 Q F -1.9306 Soluble
121 G F 0.0000 Buried
122 T F 0.0000 Buried
123 K F -2.0073 Soluble
124 V F 0.0000 Buried
125 E F -1.2983 Soluble
126 I F 0.0000 Buried
127 K F -1.2680 Soluble
128 R F -0.8791 Soluble
129 T F -0.1363 Soluble
130 V F 0.3293 Aggregation-prone
131 A F -0.0201 Soluble
132 A F -0.1273 Soluble
133 P F 0.0000 Buried
134 S F -0.2280 Soluble
135 V F 0.0000 Buried
136 F F 0.0000 Buried
137 I F 0.0000 Buried
138 F F 0.0000 Buried
139 P F -0.8742 Soluble
140 P F -0.6722 Soluble
141 S F -1.4575 Soluble
142 D F -2.2990 Soluble
143 E F -2.5472 Soluble
144 Q F 0.0000 Buried
145 L F -1.9776 Soluble
146 K F -2.6929 Soluble
147 S F -1.7410 Soluble
148 G F -1.4539 Soluble
149 T F -1.2044 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.9472 Soluble
159 F F 0.0000 Buried
160 Y F -0.9642 Soluble
161 P F -1.4525 Soluble
162 R F -2.3360 Soluble
163 E F -2.9224 Soluble
164 A F -2.1816 Soluble
165 K F -2.3882 Soluble
166 V F -1.0978 Soluble
167 Q F -0.5876 Soluble
168 W F 0.0000 Buried
169 K F -0.5872 Soluble
170 V F 0.0000 Buried
171 D F -2.1357 Soluble
172 N F -1.6104 Soluble
173 A F -0.3274 Soluble
174 L F 0.6374 Aggregation-prone
175 Q F -0.2155 Soluble
177 G F -0.8433 Soluble
178 N F -0.8307 Soluble
179 S F -1.0370 Soluble
180 Q F -1.2439 Soluble
181 E F -1.5063 Soluble
182 S F 0.0000 Buried
183 V F -0.3204 Soluble
184 T F 0.0000 Buried
185 E F -1.7394 Soluble
186 Q F 0.0000 Buried
187 D F -1.9904 Soluble
188 S F -2.2015 Soluble
189 K F -2.6077 Soluble
190 D F -1.9299 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.6019 Soluble
199 L F 0.0000 Buried
200 T F -0.4435 Soluble
201 L F -0.6822 Soluble
202 S F -1.0638 Soluble
203 K F -2.0018 Soluble
204 A F -1.7686 Soluble
205 D F -2.4130 Soluble
206 Y F 0.0000 Buried
207 E F -3.2400 Soluble
208 K F -3.4695 Soluble
209 H F -2.9632 Soluble
210 K F -3.2549 Soluble
211 V F -1.6805 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -0.6661 Soluble
216 V F 0.0000 Buried
217 T F -1.2391 Soluble
218 H F 0.0000 Buried
219 Q F -1.6643 Soluble
220 G F -0.4148 Soluble
221 L F -0.2129 Soluble
222 S F -0.4321 Soluble
223 S F -0.4193 Soluble
224 P F -0.5627 Soluble
225 V F 0.0153 Aggregation-prone
226 T F -0.3376 Soluble
227 K F -0.6377 Soluble
228 S F -0.7557 Soluble
229 F F 0.0000 Buried
230 N F -2.1145 Soluble
231 R F -2.3680 Soluble
232 G F -1.4477 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7302 3.7742 CSV PDB
4.5 -0.7713 3.7742 CSV PDB
5.0 -0.8177 3.7742 CSV PDB
5.5 -0.8602 3.7742 CSV PDB
6.0 -0.8886 3.7742 CSV PDB
6.5 -0.8943 3.7742 CSV PDB
7.0 -0.8775 3.7742 CSV PDB
7.5 -0.846 3.7741 CSV PDB
8.0 -0.8063 3.7741 CSV PDB
8.5 -0.7601 3.774 CSV PDB
9.0 -0.7068 3.7735 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH