Acimtamig / 7seg

A4DAB004_7seg_H_L | Source PDB: 7seg | Chains: H, L | Done
97.79
Yes
10 Å
Yes
Avg A4D Score
-0.6038
Max Score
1.5778
Min Score
-3.4333
Total Score
-256.01
Score CDR
-0.1096
Score non-CDR
-0.6654

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGESLKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGAIEPMYGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGSAYYYDFADYWGQGTLVTVSSASTKGPSVFPLAPSSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPSYVLTQPSSVSVAPGQTATISCGGHNIGSKNVHWYQQRPGQSPVLVIYQDNKRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCQVWDNYSVLFGGGTKLTVLRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4333
Maximum Score 1.5778
Average Score -0.6038
Total Score -256.013

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.6541 Aggregation-prone
3 Q H -0.7439 Soluble
4 L H 0.0000 Buried
5 V H -0.5111 Soluble
6 Q H 0.0000 Buried
7 S H -0.7516 Soluble
8 G H -0.6682 Soluble
9 A H -0.3490 Soluble
11 E H -0.8429 Soluble
12 V H -0.1617 Soluble
13 K H -1.3257 Soluble
14 K H -1.8877 Soluble
15 P H -2.3331 Soluble
16 G H -2.0427 Soluble
17 E H -2.2164 Soluble
18 S H -1.7981 Soluble
19 L H 0.0000 Buried
20 K H -1.9320 Soluble
21 V H 0.0000 Buried
22 S H -0.6793 Soluble
23 C H 0.0000 Buried
24 K H -1.5361 Soluble
25 A H 0.0000 Buried
26 S H -0.5096 Soluble
27 G H -0.1574 Soluble
28 Y H 0.1299 Aggregation-prone
29 T H 0.0789 Aggregation-prone
30 F H 0.0000 Buried
35 T H 0.3866 Aggregation-prone
36 S H 0.6069 Aggregation-prone
37 Y H 0.9393 Aggregation-prone
38 Y H 0.6196 Aggregation-prone
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.6271 Soluble
45 A H -1.0177 Soluble
46 P H -0.8318 Soluble
47 G H -1.2546 Soluble
48 Q H -1.7227 Soluble
49 G H -1.0770 Soluble
50 L H 0.0000 Buried
51 E H -0.4993 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 E H 0.3566 Aggregation-prone
58 P H 0.0000 Buried
59 M H 0.8433 Aggregation-prone
62 Y H 1.2822 Aggregation-prone
63 G H 0.2525 Aggregation-prone
64 S H 0.0885 Aggregation-prone
65 T H -0.0296 Soluble
66 S H -0.0502 Soluble
67 Y H -0.6039 Soluble
68 A H -1.1463 Soluble
69 Q H -2.5144 Soluble
70 K H -2.7070 Soluble
71 F H 0.0000 Buried
72 Q H -2.4042 Soluble
74 G H -1.6655 Soluble
75 R H -1.5398 Soluble
76 V H 0.0000 Buried
77 T H -0.6821 Soluble
78 M H 0.0000 Buried
79 T H -0.4494 Soluble
80 R H -0.8136 Soluble
81 D H -1.1468 Soluble
82 T H -0.4583 Soluble
83 S H -0.5643 Soluble
84 T H -0.7656 Soluble
85 S H -0.8740 Soluble
86 T H 0.0000 Buried
87 V H 0.0000 Buried
88 Y H -0.4830 Soluble
89 M H 0.0000 Buried
90 E H -1.1725 Soluble
91 L H 0.0000 Buried
92 S H -1.4378 Soluble
93 S H -1.5271 Soluble
94 L H 0.0000 Buried
95 R H -3.2233 Soluble
96 S H -2.3102 Soluble
97 E H -2.5626 Soluble
98 D H 0.0000 Buried
99 T H -0.8952 Soluble
100 A H 0.0000 Buried
101 V H 0.1203 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 G H 0.0000 Buried
108 S H 0.5184 Aggregation-prone
109 A H 0.7607 Aggregation-prone
110 Y H 1.5778 Aggregation-prone
111 Y H 1.0439 Aggregation-prone
112 Y H 0.0000 Buried
113 D H -0.1736 Soluble
114 F H 0.0000 Buried
115 A H 0.0000 Buried
116 D H 0.2398 Aggregation-prone
117 Y H 0.7225 Aggregation-prone
118 W H 0.2704 Aggregation-prone
119 G H 0.0000 Buried
120 Q H -1.3990 Soluble
121 G H -0.6270 Soluble
122 T H 0.0000 Buried
123 L H 0.0334 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1240 Soluble
128 S H -0.8236 Soluble
129 A H -0.4629 Soluble
130 S H -0.5417 Soluble
131 T H -0.5172 Soluble
132 K H -1.1818 Soluble
133 G H -1.3320 Soluble
134 P H -0.6099 Soluble
135 S H -0.4119 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1833 Soluble
139 L H 0.0000 Buried
140 A H -0.8369 Soluble
141 P H 0.0000 Buried
142 S H -0.3176 Soluble
143 S H -0.3815 Soluble
144 T H -0.3053 Soluble
145 A H -0.2143 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.2950 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.4164 Soluble
158 P H -0.7952 Soluble
159 V H 0.0000 Buried
160 T H -0.7196 Soluble
161 V H -0.4381 Soluble
162 S H -0.4397 Soluble
163 W H 0.0000 Buried
164 N H -0.6690 Soluble
165 S H -0.6544 Soluble
166 G H -0.4967 Soluble
167 A H -0.2158 Soluble
168 L H 0.0416 Aggregation-prone
169 T H -0.1562 Soluble
170 S H -0.1619 Soluble
171 G H -0.1724 Soluble
172 V H 0.1834 Aggregation-prone
173 H H -0.3030 Soluble
174 T H 0.0312 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.3239 Soluble
177 A H 0.2265 Aggregation-prone
178 V H 0.6005 Aggregation-prone
179 L H 1.4865 Aggregation-prone
180 Q H 0.4673 Aggregation-prone
181 S H -0.0039 Soluble
182 S H -0.1361 Soluble
183 G H 0.1181 Aggregation-prone
184 L H 0.2105 Aggregation-prone
185 Y H 0.5369 Aggregation-prone
186 S H 0.3530 Aggregation-prone
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1293 Soluble
193 V H 0.0000 Buried
194 P H -0.4333 Soluble
195 S H -0.5352 Soluble
196 S H -0.5855 Soluble
197 S H -0.6452 Soluble
198 L H -0.8829 Soluble
199 G H -1.0243 Soluble
200 T H -0.7363 Soluble
201 Q H -1.2389 Soluble
202 T H -1.1326 Soluble
203 Y H 0.0000 Buried
204 I H -1.1331 Soluble
205 C H 0.0000 Buried
206 N H -1.4513 Soluble
207 V H 0.0000 Buried
208 N H -2.1810 Soluble
209 H H 0.0000 Buried
210 K H -2.8701 Soluble
211 P H -1.6763 Soluble
212 S H -1.9080 Soluble
213 N H -2.6659 Soluble
214 T H -2.1298 Soluble
215 K H -2.6902 Soluble
216 V H -1.4641 Soluble
217 D H -2.3896 Soluble
218 K H -1.7698 Soluble
219 K H -2.3036 Soluble
220 V H 0.0000 Buried
221 E H -2.4274 Soluble
222 P H -1.2341 Soluble
1 S L 0.0205 Aggregation-prone
2 Y L 0.6217 Aggregation-prone
3 V L 1.5123 Aggregation-prone
4 L L 0.5666 Aggregation-prone
5 T L 0.0359 Aggregation-prone
6 Q L -0.2891 Soluble
7 P L -0.6493 Soluble
8 S L -0.8387 Soluble
9 S L -0.8780 Soluble
11 V L -0.3962 Soluble
12 S L -0.2537 Soluble
13 V L 0.0000 Buried
14 A L -0.6239 Soluble
15 P L -1.0495 Soluble
16 G L -1.3516 Soluble
17 Q L -1.6622 Soluble
18 T L -1.0250 Soluble
19 A L 0.0000 Buried
20 T L -0.1403 Soluble
21 I L 0.0000 Buried
22 S L -0.3685 Soluble
23 C L 0.0000 Buried
24 G L -0.7664 Soluble
25 G L 0.0000 Buried
26 H L -1.8357 Soluble
27 N L -2.6165 Soluble
28 I L 0.0000 Buried
29 G L -1.9883 Soluble
36 S L -1.5021 Soluble
37 K L -1.3105 Soluble
38 N L -1.5729 Soluble
39 V L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 R L -1.1611 Soluble
46 P L -1.6618 Soluble
47 G L -1.3372 Soluble
48 Q L -1.8103 Soluble
49 S L -0.8978 Soluble
50 P L 0.0000 Buried
51 V L 0.7100 Aggregation-prone
52 L L 0.0000 Buried
53 V L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -1.2568 Soluble
56 Q L -1.7842 Soluble
57 D L -2.0247 Soluble
65 N L -2.5908 Soluble
66 K L -2.5690 Soluble
67 R L -2.1668 Soluble
68 P L -1.0537 Soluble
69 S L -0.8885 Soluble
70 G L -0.7989 Soluble
71 I L -0.6238 Soluble
72 P L -1.2387 Soluble
74 E L -2.1987 Soluble
75 R L -1.2946 Soluble
76 F L 0.0000 Buried
77 S L -1.4411 Soluble
78 G L -1.3198 Soluble
79 S L -1.2702 Soluble
80 N L -1.4323 Soluble
83 S L -1.3074 Soluble
84 G L -1.9703 Soluble
85 N L -2.4997 Soluble
86 T L -1.4030 Soluble
87 A L 0.0000 Buried
88 T L -0.4818 Soluble
89 L L 0.0000 Buried
90 T L -0.2668 Soluble
91 I L 0.0000 Buried
92 S L -1.1075 Soluble
93 G L -1.0915 Soluble
94 T L 0.0000 Buried
95 Q L -0.8850 Soluble
96 A L -0.5299 Soluble
97 M L 0.5915 Aggregation-prone
98 D L 0.0000 Buried
99 E L -0.8895 Soluble
100 A L 0.0000 Buried
101 D L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 V L 0.0000 Buried
107 W L -0.1323 Soluble
108 D L -0.3853 Soluble
109 N L -0.6927 Soluble
114 Y L 0.6415 Aggregation-prone
115 S L 0.0936 Aggregation-prone
116 V L 0.0000 Buried
117 L L 0.6291 Aggregation-prone
118 F L 0.4335 Aggregation-prone
119 G L 0.0000 Buried
120 G L -0.7544 Soluble
121 G L -0.9693 Soluble
122 T L 0.0000 Buried
123 K L -1.9883 Soluble
124 L L 0.0000 Buried
125 T L -0.6450 Soluble
126 V L 0.0000 Buried
127 L L 0.4368 Aggregation-prone
128 R L -0.2810 Soluble
129 T L 0.1563 Aggregation-prone
130 V L 0.4038 Aggregation-prone
131 A L -0.1479 Soluble
132 A L -0.2168 Soluble
133 P L 0.0000 Buried
134 S L -0.2540 Soluble
135 V L 0.0000 Buried
136 F L 0.0235 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6335 Soluble
140 P L -0.8472 Soluble
141 S L -1.7503 Soluble
142 D L -3.0494 Soluble
143 E L -3.2582 Soluble
144 Q L 0.0000 Buried
145 L L -2.3378 Soluble
146 K L -2.8800 Soluble
147 S L -1.8491 Soluble
148 G L -1.2962 Soluble
149 T L -1.0276 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9351 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.2261 Soluble
162 R L -2.2953 Soluble
163 E L -2.9652 Soluble
164 A L -2.2063 Soluble
165 K L -2.2789 Soluble
166 V L -1.1459 Soluble
167 Q L -0.5720 Soluble
168 W L 0.0000 Buried
169 K L -0.4498 Soluble
170 V L 0.0000 Buried
171 D L -1.7333 Soluble
172 N L -1.3901 Soluble
173 A L -0.1933 Soluble
174 L L 0.7809 Aggregation-prone
175 Q L -0.1027 Soluble
176 S L -0.3756 Soluble
177 G L -0.8043 Soluble
178 N L -0.6688 Soluble
179 S L -0.9449 Soluble
180 Q L -1.1014 Soluble
181 E L -1.7353 Soluble
182 S L -0.6639 Soluble
183 V L -0.5244 Soluble
184 T L 0.0000 Buried
185 E L -2.0952 Soluble
186 Q L 0.0000 Buried
187 D L -1.8673 Soluble
188 S L -1.6062 Soluble
189 K L -2.2234 Soluble
190 D L -1.7956 Soluble
191 S L -1.4565 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5212 Soluble
199 L L 0.0000 Buried
200 T L -0.3454 Soluble
201 L L -0.5925 Soluble
202 S L -1.1827 Soluble
203 K L -2.0467 Soluble
204 A L -1.7808 Soluble
205 D L -2.4190 Soluble
206 Y L 0.0000 Buried
207 E L -3.1645 Soluble
208 K L -3.4333 Soluble
209 H L -2.7499 Soluble
210 K L -2.7157 Soluble
211 V L -1.1779 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9111 Soluble
216 V L 0.0000 Buried
217 T L -1.2474 Soluble
218 H L 0.0000 Buried
219 Q L -1.7489 Soluble
220 G L -0.5083 Soluble
221 L L -0.2899 Soluble
222 S L -0.4686 Soluble
223 S L -0.4346 Soluble
224 P L -0.6529 Soluble
225 V L -0.1315 Soluble
226 T L -0.5421 Soluble
227 K L -0.7730 Soluble
228 S L -0.6399 Soluble
229 F L -0.8286 Soluble
230 N L -1.7925 Soluble
231 R L -1.9735 Soluble
232 G L -1.2725 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5923 2.0027 CSV PDB
4.5 -0.6277 1.9883 CSV PDB
5.0 -0.669 1.9677 CSV PDB
5.5 -0.707 1.9439 CSV PDB
6.0 -0.7317 1.9198 CSV PDB
6.5 -0.7349 1.8972 CSV PDB
7.0 -0.717 1.8766 CSV PDB
7.5 -0.6857 1.8572 CSV PDB
8.0 -0.6474 1.8382 CSV PDB
8.5 -0.6041 1.8188 CSV PDB
9.0 -0.5566 1.8397 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH