Fidasimtamab / 6bhz

A4DAB118_6bhz_I_M | Source PDB: 6bhz | Chains: I, M | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6908
Max Score
2.3177
Min Score
-3.2394
Total Score
-283.9
Score CDR
-0.3959
Score non-CDR
-0.7545

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYRWAMDYWGQGTLVTVSSASKGPSVFPLAGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESTEQDSKDSTYSLSSTLTLSKAAYEKHKVYAEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) I (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2394
Maximum Score 2.3177
Average Score -0.6908
Total Score -283.905

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E I -2.0966 Soluble
2 V I -1.2110 Soluble
3 Q I -1.0298 Soluble
4 L I 0.2710 Aggregation-prone
5 V I 0.9227 Aggregation-prone
6 E I 0.0869 Aggregation-prone
7 S I -0.4961 Soluble
8 G I -0.9499 Soluble
9 G I -0.4791 Soluble
11 G I 0.0257 Aggregation-prone
12 L I 0.2427 Aggregation-prone
13 V I -0.5003 Soluble
14 Q I -1.5520 Soluble
15 P I -1.7310 Soluble
16 G I -1.4619 Soluble
17 G I -1.1608 Soluble
18 S I -1.1835 Soluble
19 L I -1.2236 Soluble
20 R I -2.2181 Soluble
21 L I 0.0000 Buried
24 A I -0.0192 Soluble
25 A I -0.2254 Soluble
26 S I -0.6506 Soluble
27 G I -1.0967 Soluble
28 F I -0.6333 Soluble
30 I I 0.0000 Buried
35 K I -1.7650 Soluble
36 D I -1.9525 Soluble
37 T I 0.0000 Buried
38 Y I 0.0627 Aggregation-prone
39 I I 0.0000 Buried
40 H I 0.0000 Buried
41 W I 0.0000 Buried
42 V I 0.0000 Buried
43 R I 0.0000 Buried
44 Q I -0.7993 Soluble
45 A I -1.1538 Soluble
46 P I -0.9438 Soluble
47 G I -1.4940 Soluble
48 K I -2.4144 Soluble
49 G I -1.6839 Soluble
50 L I 0.0000 Buried
51 E I -1.0930 Soluble
52 W I 0.0000 Buried
53 V I 0.0000 Buried
54 A I 0.0000 Buried
55 R I -0.6305 Soluble
56 I I 0.0000 Buried
57 Y I -0.2891 Soluble
58 P I -0.7374 Soluble
59 T I -0.9941 Soluble
62 N I -0.8282 Soluble
63 G I -0.2736 Soluble
64 Y I 0.5047 Aggregation-prone
65 T I -0.4762 Soluble
66 R I -2.0498 Soluble
67 Y I -1.9469 Soluble
68 A I 0.0000 Buried
69 D I -2.6924 Soluble
70 S I -1.7128 Soluble
71 V I 0.0000 Buried
72 K I -2.8655 Soluble
74 G I -1.7111 Soluble
75 R I -1.4304 Soluble
76 F I 0.0000 Buried
77 T I -1.1754 Soluble
78 I I 0.0000 Buried
79 S I -0.1674 Soluble
80 A I -0.6863 Soluble
81 D I -1.3712 Soluble
82 T I -1.2809 Soluble
83 S I -1.3830 Soluble
84 K I -2.0958 Soluble
85 N I -1.3523 Soluble
86 T I -0.6961 Soluble
87 A I -0.3760 Soluble
88 Y I -0.5603 Soluble
89 L I 0.0000 Buried
90 Q I -1.5379 Soluble
91 M I 0.0000 Buried
92 N I -1.3865 Soluble
93 S I -1.2290 Soluble
94 L I 0.0000 Buried
95 R I -2.3570 Soluble
96 A I -1.7540 Soluble
97 E I -2.2692 Soluble
98 D I 0.0000 Buried
99 T I -0.6558 Soluble
100 A I 0.0000 Buried
101 V I 0.2900 Aggregation-prone
102 Y I 0.0000 Buried
103 Y I 0.0571 Aggregation-prone
106 R I -0.0655 Soluble
107 W I 0.1381 Aggregation-prone
108 A I 0.1868 Aggregation-prone
115 M I 0.0000 Buried
116 D I -0.2466 Soluble
117 Y I -0.2345 Soluble
118 W I -0.3642 Soluble
119 G I 0.0000 Buried
120 Q I -1.3118 Soluble
121 G I -0.5281 Soluble
122 T I -0.0074 Soluble
123 L I 0.3815 Aggregation-prone
124 V I 0.0000 Buried
125 T I -0.3147 Soluble
126 V I 0.0000 Buried
127 S I -0.9212 Soluble
128 S I -0.6747 Soluble
129 A I -0.5342 Soluble
130 S I -0.7090 Soluble
132 K I -1.8526 Soluble
133 G I -1.5961 Soluble
134 P I 0.0000 Buried
135 S I -0.3087 Soluble
136 V I 0.0000 Buried
137 F I 0.0000 Buried
138 P I -1.0871 Soluble
139 L I 0.0000 Buried
140 A I -0.6487 Soluble
141 G I -0.6656 Soluble
142 T I -0.4978 Soluble
143 A I -0.2100 Soluble
144 A I 0.0222 Aggregation-prone
145 L I 0.0000 Buried
146 G I 0.0000 Buried
147 C I 0.0000 Buried
148 L I 0.0000 Buried
149 V I 0.0000 Buried
150 K I 0.0000 Buried
151 D I -0.4114 Soluble
152 Y I 0.0000 Buried
153 F I 0.0000 Buried
154 P I 0.0000 Buried
155 E I -0.6597 Soluble
156 P I -0.7164 Soluble
157 V I -0.5500 Soluble
158 T I -0.4220 Soluble
159 V I -0.0211 Soluble
160 S I -0.3121 Soluble
161 W I 0.0000 Buried
162 N I -0.7360 Soluble
163 S I -0.6554 Soluble
164 G I -0.5525 Soluble
165 A I -0.2769 Soluble
166 L I -0.0177 Soluble
167 T I -0.1885 Soluble
168 S I -0.1823 Soluble
169 G I -0.2059 Soluble
170 V I 0.1760 Aggregation-prone
171 H I -0.3018 Soluble
172 T I -0.0294 Soluble
173 F I 0.0000 Buried
174 P I -0.3333 Soluble
175 A I 0.2429 Aggregation-prone
176 V I 0.6424 Aggregation-prone
177 L I 1.3788 Aggregation-prone
178 Q I 0.3781 Aggregation-prone
179 S I -0.0373 Soluble
180 S I -0.1796 Soluble
181 G I 0.1039 Aggregation-prone
182 L I 0.1238 Aggregation-prone
183 Y I 0.4549 Aggregation-prone
184 S I 0.0000 Buried
185 L I 0.0000 Buried
186 S I 0.0000 Buried
187 S I 0.0000 Buried
188 V I 0.0000 Buried
189 V I 0.0000 Buried
190 T I -0.1105 Soluble
191 V I 0.0000 Buried
192 P I -0.6313 Soluble
193 S I -0.5591 Soluble
194 S I -0.6615 Soluble
195 S I 0.0000 Buried
196 L I -0.8606 Soluble
197 G I -1.0736 Soluble
198 T I -0.8609 Soluble
199 Q I -1.5050 Soluble
200 T I -1.2490 Soluble
201 Y I 0.0000 Buried
202 I I -1.0469 Soluble
203 C I 0.0000 Buried
204 N I -1.2108 Soluble
205 V I 0.0000 Buried
207 H I 0.0000 Buried
208 K I -2.6621 Soluble
209 P I -1.6503 Soluble
210 S I -1.9867 Soluble
211 N I -2.5950 Soluble
212 T I -2.0802 Soluble
213 K I -2.4474 Soluble
214 V I -1.2988 Soluble
215 D I -1.9901 Soluble
216 K I -1.6819 Soluble
217 K I -2.1792 Soluble
218 V I 0.0000 Buried
219 E I -2.4240 Soluble
220 P I -1.2212 Soluble
1 D M -2.2596 Soluble
2 I M 0.0000 Buried
3 Q M -2.3060 Soluble
4 M M 0.0000 Buried
5 T M -1.4135 Soluble
6 Q M 0.0000 Buried
7 S M -0.8419 Soluble
8 P M -0.5865 Soluble
9 S M -0.7921 Soluble
10 S M -0.6463 Soluble
11 L M -0.4211 Soluble
12 S M -0.7508 Soluble
13 A M 0.0000 Buried
14 S M -0.5694 Soluble
15 V M 0.1258 Aggregation-prone
16 G M -0.6893 Soluble
17 D M -1.4735 Soluble
18 R M -2.1959 Soluble
19 V M 0.0000 Buried
20 T M -0.6092 Soluble
21 I M 0.0000 Buried
22 T M -0.7792 Soluble
23 C M 0.0000 Buried
24 R M -2.8725 Soluble
25 A M 0.0000 Buried
26 S M -2.2176 Soluble
27 Q M -2.6808 Soluble
28 D M -2.5751 Soluble
29 V M 0.0000 Buried
36 N M -1.7590 Soluble
37 T M -0.8519 Soluble
38 A M -0.1340 Soluble
39 V M 0.0000 Buried
40 A M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M 0.0000 Buried
44 Q M 0.0000 Buried
45 K M -1.7076 Soluble
46 P M -1.5650 Soluble
47 G M -1.6650 Soluble
48 K M -2.6066 Soluble
49 A M -1.6164 Soluble
50 P M 0.0000 Buried
51 K M -1.5738 Soluble
52 L M 0.0000 Buried
53 L M 0.0000 Buried
54 I M 0.0000 Buried
55 Y M 1.2919 Aggregation-prone
56 S M 0.5068 Aggregation-prone
57 A M 0.0000 Buried
65 S M 0.8017 Aggregation-prone
66 F M 2.3177 Aggregation-prone
67 L M 1.5770 Aggregation-prone
68 Y M 0.9234 Aggregation-prone
69 S M 0.0658 Aggregation-prone
70 G M -0.3748 Soluble
71 V M -0.1944 Soluble
72 P M -0.2460 Soluble
74 S M -0.3467 Soluble
75 R M -0.6795 Soluble
76 F M 0.0000 Buried
77 S M 0.2075 Aggregation-prone
78 G M 0.1506 Aggregation-prone
79 S M -0.5375 Soluble
80 R M -1.6111 Soluble
83 S M -1.3518 Soluble
84 G M -1.8041 Soluble
85 T M -2.2614 Soluble
86 D M -2.1402 Soluble
87 F M 0.0000 Buried
88 T M -0.7852 Soluble
89 L M 0.0000 Buried
90 T M -0.6038 Soluble
91 I M 0.0000 Buried
92 S M -1.2793 Soluble
93 S M -1.0332 Soluble
94 L M 0.0000 Buried
95 Q M -0.8893 Soluble
96 P M -0.9960 Soluble
97 E M -1.9479 Soluble
98 D M 0.0000 Buried
99 F M -0.6511 Soluble
100 A M 0.0000 Buried
101 T M -0.7502 Soluble
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 Q M 0.0000 Buried
106 Q M 0.0000 Buried
107 H M 0.2661 Aggregation-prone
108 Y M 0.5504 Aggregation-prone
109 T M 0.1647 Aggregation-prone
114 T M -0.5153 Soluble
115 P M -0.9695 Soluble
116 P M 0.0000 Buried
117 T M -0.7646 Soluble
118 F M -0.5813 Soluble
119 G M 0.0000 Buried
120 Q M -1.7720 Soluble
121 G M 0.0000 Buried
122 T M 0.0000 Buried
123 K M -1.2027 Soluble
124 V M 0.0000 Buried
125 E M -0.9820 Soluble
126 I M -0.6032 Soluble
127 K M -1.2289 Soluble
128 R M -0.8908 Soluble
129 T M -0.1257 Soluble
130 V M 0.2673 Aggregation-prone
131 A M 0.1388 Aggregation-prone
132 A M -0.1144 Soluble
133 P M 0.0000 Buried
134 S M -0.1575 Soluble
135 V M 0.0000 Buried
136 F M 0.0685 Aggregation-prone
137 I M 0.1030 Aggregation-prone
138 F M 0.0000 Buried
139 P M -0.7389 Soluble
140 P M -0.9036 Soluble
141 S M -1.6256 Soluble
142 D M -2.9135 Soluble
143 E M -2.8114 Soluble
144 Q M 0.0000 Buried
145 L M -2.1748 Soluble
146 K M -2.7988 Soluble
147 S M -1.7163 Soluble
148 G M -1.2108 Soluble
149 T M -0.8598 Soluble
150 A M 0.0000 Buried
151 S M 0.0000 Buried
152 V M 0.0000 Buried
153 V M 0.0000 Buried
154 C M 0.0000 Buried
155 L M 0.0000 Buried
156 L M 0.0000 Buried
157 N M 0.0000 Buried
158 N M -0.8024 Soluble
159 F M 0.0000 Buried
160 Y M -1.1122 Soluble
161 P M -1.7291 Soluble
162 R M -2.9522 Soluble
163 E M -3.2394 Soluble
164 A M -2.3018 Soluble
165 K M -2.2843 Soluble
166 V M -1.1313 Soluble
167 Q M -0.8385 Soluble
168 W M 0.0000 Buried
169 K M -0.8134 Soluble
170 V M 0.0000 Buried
171 D M -1.9754 Soluble
172 N M -1.7066 Soluble
173 A M -0.5134 Soluble
174 L M 0.2140 Aggregation-prone
175 Q M -0.5002 Soluble
176 S M -0.7364 Soluble
177 G M -1.2335 Soluble
178 N M -1.4977 Soluble
179 S M -1.3790 Soluble
180 Q M -1.3611 Soluble
181 E M -1.6208 Soluble
182 S M -0.7842 Soluble
184 T M -1.3103 Soluble
185 E M -2.3484 Soluble
186 Q M -1.9301 Soluble
187 D M -1.9003 Soluble
188 S M -1.8839 Soluble
189 K M -2.2163 Soluble
190 D M -1.6271 Soluble
191 S M -1.6689 Soluble
192 T M 0.0000 Buried
193 Y M 0.0000 Buried
194 S M 0.0000 Buried
195 L M -0.6362 Soluble
196 S M 0.0000 Buried
197 S M 0.0000 Buried
198 T M -0.6920 Soluble
199 L M 0.0000 Buried
200 T M -0.4884 Soluble
201 L M -0.4286 Soluble
202 S M -0.6331 Soluble
203 K M -1.6041 Soluble
204 A M -1.1571 Soluble
205 A M -1.2020 Soluble
206 Y M 0.0000 Buried
207 E M -2.7416 Soluble
208 K M -2.9587 Soluble
209 H M -2.7538 Soluble
210 K M -3.0820 Soluble
211 V M -1.5435 Soluble
212 Y M 0.0000 Buried
213 A M -0.9441 Soluble
215 E M -0.8891 Soluble
216 V M 0.0000 Buried
217 T M -1.2173 Soluble
218 H M 0.0000 Buried
219 Q M -1.7186 Soluble
220 G M -0.4284 Soluble
221 L M -0.2161 Soluble
222 S M -0.4404 Soluble
223 S M -0.4250 Soluble
224 P M -0.5576 Soluble
225 V M -0.0992 Soluble
226 T M -0.5982 Soluble
227 K M -0.8237 Soluble
228 S M -0.6126 Soluble
229 F M -0.9212 Soluble
230 N M -1.9297 Soluble
231 R M -2.4876 Soluble
232 G M -2.2056 Soluble
233 E M -2.0316 Soluble
234 C M -0.4245 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6878 3.944 CSV PDB
4.5 -0.7315 3.944 CSV PDB
5.0 -0.7814 3.944 CSV PDB
5.5 -0.8278 3.944 CSV PDB
6.0 -0.8594 3.944 CSV PDB
6.5 -0.8676 3.944 CSV PDB
7.0 -0.8525 3.944 CSV PDB
7.5 -0.8224 3.944 CSV PDB
8.0 -0.7841 3.9439 CSV PDB
8.5 -0.7392 3.9437 CSV PDB
9.0 -0.688 3.943 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH