Fidasimtamab / 1fve

A4DAB118_1fve_B_A | Source PDB: 1fve | Chains: B, A | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.7416
Max Score
1.9698
Min Score
-4.0239
Total Score
-324.1
Score CDR
-0.676
Score non-CDR
-0.7496

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLESGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) B (Heavy), A (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.0239
Maximum Score 1.9698
Average Score -0.7416
Total Score -324.0961

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E B -2.1792 Soluble
2 V B -1.4929 Soluble
3 Q B -1.4729 Soluble
4 L B 0.0000 Buried
5 V B 0.4335 Aggregation-prone
6 E B 0.0000 Buried
7 S B -0.5752 Soluble
8 G B -0.9709 Soluble
9 G B -0.4960 Soluble
11 G B -0.1459 Soluble
12 L B 0.0962 Aggregation-prone
13 V B -0.5929 Soluble
14 Q B -1.6023 Soluble
15 P B -1.8053 Soluble
16 G B -1.5255 Soluble
17 G B -1.1224 Soluble
18 S B -1.1162 Soluble
19 L B -1.1808 Soluble
20 R B -2.1295 Soluble
21 L B 0.0000 Buried
22 S B -0.4966 Soluble
23 C B 0.0000 Buried
24 A B -0.2076 Soluble
25 A B 0.0000 Buried
26 S B -1.0949 Soluble
27 G B -1.5402 Soluble
28 F B -1.5695 Soluble
29 N B -2.4703 Soluble
30 I B 0.0000 Buried
35 K B -2.9842 Soluble
36 D B -2.7477 Soluble
37 T B 0.0000 Buried
38 Y B -0.2330 Soluble
39 I B 0.0000 Buried
40 H B 0.0000 Buried
41 W B 0.0000 Buried
42 V B 0.0000 Buried
43 R B 0.0000 Buried
44 Q B -0.9185 Soluble
45 A B -1.2090 Soluble
46 P B -0.9227 Soluble
47 G B -1.4786 Soluble
48 K B -2.4807 Soluble
49 G B -1.8069 Soluble
50 L B 0.0000 Buried
51 E B -1.4617 Soluble
52 W B 0.0000 Buried
53 V B 0.0000 Buried
54 A B 0.0000 Buried
55 R B -0.5457 Soluble
56 I B 0.0000 Buried
57 Y B -0.4518 Soluble
58 P B -1.2222 Soluble
59 T B -1.3494 Soluble
62 N B -1.0462 Soluble
63 G B -0.4263 Soluble
64 Y B 0.4729 Aggregation-prone
65 T B -0.4455 Soluble
66 R B -1.9975 Soluble
67 Y B -1.9081 Soluble
68 A B -1.9460 Soluble
69 D B -2.7588 Soluble
70 S B -1.7967 Soluble
71 V B 0.0000 Buried
72 K B -2.9251 Soluble
74 G B -1.7463 Soluble
75 R B -1.4915 Soluble
76 F B 0.0000 Buried
77 T B -1.1122 Soluble
78 I B 0.0000 Buried
79 S B -0.2036 Soluble
80 A B -0.9151 Soluble
81 D B -1.6901 Soluble
82 T B -2.0531 Soluble
83 S B -1.7247 Soluble
84 K B -2.3453 Soluble
85 N B -1.9882 Soluble
86 T B 0.0000 Buried
87 A B 0.0000 Buried
88 Y B -0.5924 Soluble
89 L B 0.0000 Buried
90 Q B -1.2681 Soluble
91 M B 0.0000 Buried
92 N B -1.4100 Soluble
93 S B -1.3417 Soluble
94 L B 0.0000 Buried
95 R B -2.7577 Soluble
96 A B -1.9481 Soluble
97 E B -2.4259 Soluble
98 D B 0.0000 Buried
99 T B -0.7066 Soluble
100 A B 0.0000 Buried
101 V B 0.2647 Aggregation-prone
102 Y B 0.0000 Buried
103 Y B 0.0000 Buried
104 C B 0.0000 Buried
105 S B 0.0000 Buried
106 R B -0.0276 Soluble
107 W B 0.0000 Buried
108 G B -0.8144 Soluble
109 G B -1.1936 Soluble
110 D B -1.9578 Soluble
111 G B -1.1382 Soluble
112 F B -0.2471 Soluble
113 Y B 0.4581 Aggregation-prone
114 A B 0.0000 Buried
115 M B 0.0000 Buried
116 D B -0.3089 Soluble
117 Y B -0.2550 Soluble
118 W B 0.0000 Buried
119 G B 0.0000 Buried
120 Q B -1.4441 Soluble
121 G B 0.0000 Buried
122 T B -0.0032 Soluble
123 L B 0.2147 Aggregation-prone
124 V B 0.0000 Buried
125 T B -0.3791 Soluble
126 V B 0.0000 Buried
127 S B -0.9813 Soluble
128 S B -0.6891 Soluble
129 A B -0.5374 Soluble
130 S B -0.7333 Soluble
131 T B -1.0182 Soluble
132 K B -1.8165 Soluble
133 G B -1.7099 Soluble
134 P B 0.0000 Buried
135 S B -0.5139 Soluble
136 V B 0.0000 Buried
137 F B 0.0000 Buried
138 P B -0.8130 Soluble
139 L B 0.0000 Buried
140 A B 0.0000 Buried
141 P B 0.0000 Buried
142 S B -0.9633 Soluble
143 S B 0.0000 Buried
144 K B -2.0191 Soluble
145 S B -1.2382 Soluble
146 T B -0.9843 Soluble
147 S B -0.8962 Soluble
148 G B -0.9366 Soluble
149 G B -0.9031 Soluble
150 T B -0.5907 Soluble
151 A B -0.3349 Soluble
152 A B 0.0000 Buried
153 L B 0.0000 Buried
154 G B 0.0000 Buried
155 C B 0.0000 Buried
156 L B 0.0000 Buried
157 V B 0.0000 Buried
158 K B 0.0000 Buried
159 D B -0.8230 Soluble
160 Y B 0.0000 Buried
161 F B 0.0000 Buried
162 P B 0.0000 Buried
163 E B -0.7693 Soluble
164 P B -0.7908 Soluble
165 V B -0.7246 Soluble
166 T B -0.5649 Soluble
167 V B -0.1345 Soluble
168 S B -0.3636 Soluble
169 W B 0.0000 Buried
170 N B -0.8515 Soluble
171 S B -0.6922 Soluble
172 G B -0.5992 Soluble
173 A B -0.2710 Soluble
174 L B -0.0142 Soluble
175 T B -0.1740 Soluble
176 S B -0.1493 Soluble
177 G B -0.1913 Soluble
178 V B 0.2160 Aggregation-prone
179 H B -0.1216 Soluble
180 T B 0.0384 Aggregation-prone
181 F B 0.0000 Buried
182 P B -0.3517 Soluble
183 A B 0.2395 Aggregation-prone
184 V B 0.5344 Aggregation-prone
185 L B 1.3002 Aggregation-prone
186 Q B 0.3261 Aggregation-prone
187 S B -0.1740 Soluble
188 S B -0.2397 Soluble
189 G B 0.0418 Aggregation-prone
190 L B -0.0844 Soluble
191 Y B 0.2103 Aggregation-prone
192 S B 0.0000 Buried
193 L B 0.0000 Buried
194 S B 0.0000 Buried
195 S B 0.0000 Buried
196 V B 0.0000 Buried
197 V B 0.0000 Buried
198 T B -0.1028 Soluble
199 V B 0.0000 Buried
200 P B -0.4754 Soluble
201 S B -0.5859 Soluble
202 S B -0.6158 Soluble
203 S B -0.5453 Soluble
204 L B -0.5328 Soluble
205 G B -0.8689 Soluble
206 T B -0.7511 Soluble
207 Q B -1.1766 Soluble
208 T B -1.0182 Soluble
209 Y B 0.0000 Buried
210 I B -1.2275 Soluble
211 C B 0.0000 Buried
212 N B -1.4976 Soluble
213 V B 0.0000 Buried
214 N B -1.8234 Soluble
215 H B 0.0000 Buried
216 K B -2.7280 Soluble
217 P B -1.7190 Soluble
218 S B -1.9402 Soluble
219 N B -2.5813 Soluble
220 T B -2.3133 Soluble
221 K B -2.6747 Soluble
222 V B -1.7997 Soluble
223 D B -2.5687 Soluble
224 K B -2.1169 Soluble
225 K B -2.2752 Soluble
226 V B 0.0000 Buried
227 E B -1.4451 Soluble
228 P B -1.0434 Soluble
229 K B -1.1658 Soluble
230 S B -0.8447 Soluble
231 C B -0.9486 Soluble
1 D A -2.2602 Soluble
2 I A 0.0000 Buried
3 Q A -2.3018 Soluble
4 M A 0.0000 Buried
5 T A -1.3960 Soluble
6 Q A 0.0000 Buried
7 S A -0.8058 Soluble
8 P A -0.5493 Soluble
9 S A -0.7023 Soluble
10 S A -0.4783 Soluble
11 L A -0.4254 Soluble
12 S A -0.7091 Soluble
13 A A -0.9201 Soluble
14 S A -0.8054 Soluble
15 V A 0.0310 Aggregation-prone
16 G A -0.6922 Soluble
17 D A -1.5721 Soluble
18 R A -2.2992 Soluble
19 V A 0.0000 Buried
20 T A -0.6159 Soluble
21 I A 0.0000 Buried
22 T A -0.8028 Soluble
23 C A 0.0000 Buried
24 R A -2.8022 Soluble
25 A A 0.0000 Buried
26 S A -2.2270 Soluble
27 Q A -2.7736 Soluble
28 D A -2.9490 Soluble
29 V A 0.0000 Buried
36 N A -2.0950 Soluble
37 T A -1.1245 Soluble
38 A A -0.3147 Soluble
39 V A 0.0000 Buried
40 A A 0.0000 Buried
41 W A 0.0000 Buried
42 Y A 0.0000 Buried
43 Q A 0.0000 Buried
44 Q A 0.0000 Buried
45 K A -2.8032 Soluble
46 P A -1.8590 Soluble
47 G A -1.8850 Soluble
48 K A -2.9310 Soluble
49 A A -1.9380 Soluble
50 P A 0.0000 Buried
51 K A -2.0984 Soluble
52 L A 0.0000 Buried
53 L A 0.0000 Buried
54 I A 0.0000 Buried
55 Y A 0.8661 Aggregation-prone
56 S A 0.3816 Aggregation-prone
57 A A 0.0000 Buried
65 S A 0.5736 Aggregation-prone
66 F A 1.9698 Aggregation-prone
67 L A 1.0260 Aggregation-prone
68 E A 0.0132 Aggregation-prone
69 S A -0.2055 Soluble
70 G A -0.5469 Soluble
71 V A -0.2453 Soluble
72 P A -0.3865 Soluble
74 S A -0.4721 Soluble
75 R A -0.7998 Soluble
76 F A 0.0000 Buried
77 S A 0.0555 Aggregation-prone
78 G A -0.0080 Soluble
79 S A -0.6837 Soluble
80 R A -1.8322 Soluble
83 S A -1.4073 Soluble
84 G A -1.8672 Soluble
85 T A -2.2565 Soluble
86 D A -1.8718 Soluble
87 F A 0.0000 Buried
88 T A -0.7584 Soluble
89 L A 0.0000 Buried
90 T A -0.6068 Soluble
91 I A 0.0000 Buried
92 S A -1.3737 Soluble
93 S A -1.1604 Soluble
94 L A 0.0000 Buried
95 Q A -1.1474 Soluble
96 P A -1.4490 Soluble
97 E A -2.2469 Soluble
98 D A 0.0000 Buried
99 F A -1.2997 Soluble
100 A A 0.0000 Buried
101 T A -0.8630 Soluble
102 Y A 0.0000 Buried
103 Y A 0.0000 Buried
104 C A 0.0000 Buried
105 Q A 0.0000 Buried
106 Q A 0.0000 Buried
107 H A 0.0000 Buried
108 Y A 0.3513 Aggregation-prone
109 T A 0.0046 Aggregation-prone
114 T A -0.5458 Soluble
115 P A -0.9767 Soluble
116 P A 0.0000 Buried
117 T A -0.8049 Soluble
118 F A -0.5805 Soluble
119 G A 0.0000 Buried
120 Q A -1.7692 Soluble
121 G A 0.0000 Buried
122 T A 0.0000 Buried
123 K A -0.9924 Soluble
124 V A 0.0000 Buried
125 E A 0.0000 Buried
126 I A 0.0000 Buried
127 K A -1.5531 Soluble
128 R A -0.9480 Soluble
129 T A -0.0167 Soluble
130 V A 0.6840 Aggregation-prone
131 A A 0.1793 Aggregation-prone
132 A A -0.1270 Soluble
133 P A 0.0000 Buried
134 S A -0.1979 Soluble
135 V A 0.0000 Buried
136 F A 0.1363 Aggregation-prone
137 I A -0.0714 Soluble
138 F A 0.0000 Buried
139 P A 0.0000 Buried
140 P A 0.0000 Buried
141 S A -1.6151 Soluble
142 D A -3.1473 Soluble
143 E A -2.2277 Soluble
144 Q A 0.0000 Buried
145 L A 0.0000 Buried
146 K A -2.7728 Soluble
147 S A -1.7329 Soluble
148 G A -1.5202 Soluble
149 T A -1.3768 Soluble
150 A A 0.0000 Buried
151 S A 0.0000 Buried
152 V A 0.0000 Buried
153 V A 0.0000 Buried
154 C A 0.0000 Buried
155 L A 0.0000 Buried
156 L A 0.0000 Buried
157 N A 0.0000 Buried
158 N A -1.0088 Soluble
159 F A 0.0000 Buried
160 Y A 0.0000 Buried
161 P A -1.1239 Soluble
162 R A -1.4185 Soluble
163 E A -2.5763 Soluble
164 A A -2.0580 Soluble
165 K A -2.2573 Soluble
166 V A -1.2919 Soluble
167 Q A -0.6781 Soluble
168 W A 0.0000 Buried
169 K A -0.5416 Soluble
170 V A 0.0000 Buried
171 D A -1.9037 Soluble
172 N A -1.4032 Soluble
173 A A -0.4205 Soluble
174 L A 0.3752 Aggregation-prone
175 Q A -0.7908 Soluble
176 S A -0.8664 Soluble
177 G A -1.4506 Soluble
178 N A -1.8280 Soluble
179 S A -1.5477 Soluble
180 Q A -1.4355 Soluble
181 E A -1.7395 Soluble
182 S A -0.7187 Soluble
183 V A -0.4637 Soluble
184 T A -0.8467 Soluble
185 E A -1.6812 Soluble
186 Q A 0.0000 Buried
187 D A -2.1294 Soluble
188 S A -2.2215 Soluble
189 K A -2.7018 Soluble
190 D A -2.2127 Soluble
191 S A -2.0201 Soluble
192 T A 0.0000 Buried
193 Y A 0.0000 Buried
194 S A 0.0000 Buried
195 L A 0.0000 Buried
196 S A 0.0000 Buried
197 S A 0.0000 Buried
198 T A -0.7398 Soluble
199 L A 0.0000 Buried
200 T A -0.4307 Soluble
201 L A -0.9279 Soluble
202 S A -1.2790 Soluble
203 K A -2.5628 Soluble
204 A A -2.2117 Soluble
205 D A -3.1757 Soluble
206 Y A 0.0000 Buried
207 E A -4.0239 Soluble
208 K A -3.8239 Soluble
209 H A -2.9848 Soluble
210 K A -2.7665 Soluble
211 V A -1.5772 Soluble
212 Y A 0.0000 Buried
213 A A 0.0000 Buried
214 C A 0.0000 Buried
215 E A -0.6431 Soluble
216 V A 0.0000 Buried
217 T A -1.2102 Soluble
218 H A 0.0000 Buried
219 Q A -1.4691 Soluble
220 G A -0.3669 Soluble
221 L A -0.2692 Soluble
222 S A -0.5086 Soluble
223 S A -0.4198 Soluble
224 P A -0.6270 Soluble
225 V A 0.0401 Aggregation-prone
226 T A -0.3498 Soluble
227 K A -0.5420 Soluble
228 S A -0.6279 Soluble
229 F A 0.0000 Buried
230 N A -1.4535 Soluble
231 R A -1.9057 Soluble
232 G A -1.8182 Soluble
233 E A -2.1346 Soluble
234 C A -0.3553 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7721 3.6762 CSV PDB
4.5 -0.8177 3.6695 CSV PDB
5.0 -0.8688 3.6588 CSV PDB
5.5 -0.9155 3.646 CSV PDB
6.0 -0.9468 3.633 CSV PDB
6.5 -0.9545 3.622 CSV PDB
7.0 -0.9401 3.6146 CSV PDB
7.5 -0.9112 3.611 CSV PDB
8.0 -0.8737 3.6096 CSV PDB
8.5 -0.8287 3.6091 CSV PDB
9.0 -0.7756 3.6089 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH