Tazlestobart / 7su0

A4DAB258_7su0_I_M | Source PDB: 7su0 | Chains: I, M | Done
97.79
Yes
10 Å
Yes
Avg A4D Score
-0.7184
Max Score
1.9414
Min Score
-4.0608
Total Score
-302.46
Score CDR
-0.4763
Score non-CDR
-0.7481

Calculation Parameters

Chain Sequence(s) VQLVESGGGVVQPGRSLRLSCAASGFTFSHYTMHWVRQAPGKGLEWVTFISYDGHNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPEIVLTQSPGTLSLSPGERATLSCRASQEVGESELAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) I (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.0608
Maximum Score 1.9414
Average Score -0.7184
Total Score -302.4599

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V I 0.6456 Aggregation-prone
3 Q I -0.5209 Soluble
4 L I 0.0000 Buried
5 V I 0.0824 Aggregation-prone
6 E I 0.0000 Buried
7 S I -0.5934 Soluble
8 G I -0.8280 Soluble
9 G I -0.3562 Soluble
11 G I -0.0326 Soluble
12 V I 0.2394 Aggregation-prone
13 V I 0.0000 Buried
14 Q I -1.9191 Soluble
15 P I -2.1274 Soluble
16 G I -2.1984 Soluble
17 R I -2.6960 Soluble
18 S I -2.0164 Soluble
19 L I -1.5130 Soluble
20 R I -2.2543 Soluble
21 L I 0.0000 Buried
22 S I -0.6128 Soluble
23 C I 0.0000 Buried
24 A I -0.3812 Soluble
25 A I 0.0000 Buried
26 S I -0.3075 Soluble
27 G I -0.1977 Soluble
28 F I -0.0619 Soluble
29 T I -0.3521 Soluble
30 F I 0.0000 Buried
35 S I -1.0800 Soluble
36 H I -0.9497 Soluble
37 Y I 0.0000 Buried
38 T I 0.0000 Buried
39 M I 0.0000 Buried
40 H I 0.0000 Buried
41 W I 0.0000 Buried
42 V I 0.0000 Buried
43 R I 0.0000 Buried
44 Q I -0.6590 Soluble
45 A I -1.0201 Soluble
46 P I -0.8574 Soluble
47 G I -1.4583 Soluble
48 K I -2.3682 Soluble
49 G I -1.5568 Soluble
50 L I 0.0000 Buried
51 E I -1.0211 Soluble
52 W I 0.0000 Buried
53 V I 0.0000 Buried
54 T I 0.0000 Buried
55 F I 0.1604 Aggregation-prone
56 I I 0.0000 Buried
57 S I -0.9833 Soluble
58 Y I -0.8534 Soluble
59 D I -2.2862 Soluble
62 G I -1.8810 Soluble
63 H I -2.1987 Soluble
64 N I -2.0002 Soluble
65 K I -1.0149 Soluble
66 Y I -0.1306 Soluble
67 Y I -0.6634 Soluble
68 A I -1.2667 Soluble
69 D I -2.4136 Soluble
70 S I -1.8512 Soluble
71 V I 0.0000 Buried
72 K I -2.4996 Soluble
74 G I -1.7639 Soluble
75 R I -1.5692 Soluble
76 F I 0.0000 Buried
77 T I -0.9337 Soluble
78 I I 0.0000 Buried
79 S I -1.0130 Soluble
80 R I -1.3174 Soluble
81 D I -1.9571 Soluble
82 N I -2.0004 Soluble
83 S I -1.6368 Soluble
84 K I -2.3809 Soluble
85 N I -1.6996 Soluble
86 T I 0.0000 Buried
87 L I 0.0000 Buried
88 Y I -0.6967 Soluble
89 L I 0.0000 Buried
90 Q I -1.5704 Soluble
91 M I 0.0000 Buried
92 N I -1.8660 Soluble
93 S I -1.7329 Soluble
94 L I 0.0000 Buried
95 R I -2.5707 Soluble
96 A I -1.7131 Soluble
97 E I -2.2536 Soluble
98 D I 0.0000 Buried
99 T I -0.5761 Soluble
100 A I 0.0000 Buried
101 I I 0.4054 Aggregation-prone
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 A I 0.0000 Buried
106 R I 0.0000 Buried
107 T I 0.5545 Aggregation-prone
108 G I 0.9667 Aggregation-prone
109 W I 1.6914 Aggregation-prone
110 L I 1.9414 Aggregation-prone
113 G I 0.7820 Aggregation-prone
114 P I 0.0000 Buried
115 F I 0.0000 Buried
116 D I 0.0132 Aggregation-prone
117 Y I 0.3543 Aggregation-prone
118 W I -0.2136 Soluble
119 G I 0.0000 Buried
120 Q I -1.4520 Soluble
121 G I -0.5679 Soluble
122 T I -0.0110 Soluble
123 L I 0.5768 Aggregation-prone
124 V I 0.0000 Buried
125 T I 0.0000 Buried
126 V I 0.0000 Buried
127 S I -0.8390 Soluble
128 S I -0.7425 Soluble
129 A I -0.4616 Soluble
130 S I -0.5428 Soluble
131 T I -0.6523 Soluble
132 K I -1.0841 Soluble
133 G I -1.2685 Soluble
134 P I 0.0000 Buried
135 S I -0.2385 Soluble
136 V I 0.0000 Buried
137 F I 0.0000 Buried
138 P I -1.1460 Soluble
139 L I 0.0000 Buried
140 A I -0.9459 Soluble
141 P I -0.5771 Soluble
142 T I -0.2634 Soluble
143 A I -0.1595 Soluble
144 A I 0.0000 Buried
145 L I 0.0000 Buried
146 G I 0.0000 Buried
147 C I 0.0000 Buried
148 L I 0.0000 Buried
149 V I 0.0000 Buried
150 K I 0.0000 Buried
151 D I -0.2600 Soluble
152 Y I 0.0000 Buried
153 F I 0.0000 Buried
154 P I 0.0000 Buried
155 E I -0.4078 Soluble
156 P I -0.7380 Soluble
157 V I -0.4848 Soluble
158 T I -0.5636 Soluble
159 V I -0.2220 Soluble
160 S I -0.4465 Soluble
161 W I 0.0000 Buried
162 N I -0.8738 Soluble
163 S I -0.7125 Soluble
164 G I -0.5867 Soluble
165 A I -0.2274 Soluble
166 L I -0.0179 Soluble
167 T I -0.2117 Soluble
168 S I -0.2816 Soluble
169 G I -0.4171 Soluble
170 V I 0.0666 Aggregation-prone
171 H I -0.3746 Soluble
172 T I -0.2192 Soluble
173 F I 0.0000 Buried
174 P I -0.3880 Soluble
175 A I 0.2012 Aggregation-prone
176 V I 0.5067 Aggregation-prone
177 L I 1.2984 Aggregation-prone
178 Q I 0.3446 Aggregation-prone
179 S I -0.0048 Soluble
180 S I -0.1491 Soluble
181 G I 0.0598 Aggregation-prone
182 L I 0.1621 Aggregation-prone
183 Y I 0.4491 Aggregation-prone
184 S I 0.0000 Buried
185 L I 0.0000 Buried
186 S I 0.0000 Buried
187 S I 0.0000 Buried
188 V I 0.0000 Buried
189 V I 0.0000 Buried
190 T I -0.2207 Soluble
191 V I 0.0000 Buried
192 P I -0.3964 Soluble
193 S I -0.4406 Soluble
194 S I -0.5185 Soluble
195 S I -0.5668 Soluble
196 L I -0.7897 Soluble
197 G I -1.0056 Soluble
198 T I -0.6886 Soluble
199 Q I -0.9953 Soluble
200 T I -1.1615 Soluble
201 Y I 0.0000 Buried
202 I I -1.3486 Soluble
203 C I 0.0000 Buried
204 N I -1.6652 Soluble
205 V I 0.0000 Buried
206 N I -1.7720 Soluble
207 H I 0.0000 Buried
208 K I -2.7264 Soluble
209 P I -1.5851 Soluble
210 S I -1.7982 Soluble
211 N I -2.5695 Soluble
212 T I -1.9843 Soluble
213 K I -2.6105 Soluble
214 V I -1.4080 Soluble
215 D I -2.6297 Soluble
216 K I -2.1999 Soluble
217 R I -2.7833 Soluble
218 V I 0.0000 Buried
219 E I -2.4512 Soluble
220 P I -1.2100 Soluble
1 E M -1.5071 Soluble
2 I M -0.6484 Soluble
3 V M 0.5305 Aggregation-prone
4 L M 0.0000 Buried
5 T M -0.6675 Soluble
6 Q M 0.0000 Buried
7 S M -0.8432 Soluble
8 P M -0.5247 Soluble
9 G M -0.8766 Soluble
10 T M -0.4525 Soluble
11 L M -0.1869 Soluble
12 S M -0.3955 Soluble
13 L M -0.9837 Soluble
14 S M -1.5296 Soluble
15 P M -1.9144 Soluble
16 G M -2.3016 Soluble
17 E M -3.2045 Soluble
18 R M -3.3443 Soluble
19 A M 0.0000 Buried
20 T M -0.5437 Soluble
21 L M 0.0000 Buried
22 S M -0.8187 Soluble
23 C M 0.0000 Buried
24 R M -2.1547 Soluble
25 A M 0.0000 Buried
26 S M -1.2237 Soluble
27 Q M -2.2702 Soluble
28 E M -3.0697 Soluble
29 V M 0.0000 Buried
30 G M -2.3500 Soluble
36 E M -2.6522 Soluble
37 S M -1.4705 Soluble
38 E M -1.1270 Soluble
39 L M 0.0000 Buried
40 A M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M 0.0000 Buried
44 Q M 0.0000 Buried
45 K M -1.5024 Soluble
46 P M -1.3346 Soluble
47 G M -1.4843 Soluble
48 Q M -2.1012 Soluble
49 A M -1.3027 Soluble
50 P M 0.0000 Buried
51 R M -1.3801 Soluble
52 L M 0.0000 Buried
53 L M 0.0000 Buried
54 I M 0.0000 Buried
55 Y M 0.5630 Aggregation-prone
56 G M 0.0399 Aggregation-prone
57 A M 0.0000 Buried
65 F M 1.4761 Aggregation-prone
66 S M 0.3952 Aggregation-prone
67 R M -0.9813 Soluble
68 A M -0.6137 Soluble
69 T M -0.6362 Soluble
70 G M -0.9095 Soluble
71 I M 0.0000 Buried
72 P M -1.3636 Soluble
74 D M -2.4216 Soluble
75 R M -1.9558 Soluble
76 F M 0.0000 Buried
77 S M -0.3867 Soluble
78 G M 0.4823 Aggregation-prone
79 S M -0.0166 Soluble
80 G M -0.6794 Soluble
83 S M -1.4746 Soluble
84 G M -2.1930 Soluble
85 T M -2.1637 Soluble
86 D M -2.3100 Soluble
87 F M 0.0000 Buried
88 T M -0.5036 Soluble
89 L M 0.0000 Buried
90 T M -0.7978 Soluble
91 I M 0.0000 Buried
92 S M -2.4696 Soluble
93 R M -3.5465 Soluble
94 L M 0.0000 Buried
95 E M -1.8574 Soluble
96 P M -1.5025 Soluble
97 E M -2.0890 Soluble
98 D M 0.0000 Buried
99 F M -0.5175 Soluble
100 A M 0.0000 Buried
101 V M -0.2531 Soluble
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 Q M 0.0000 Buried
106 Q M 0.0000 Buried
107 Y M 0.0000 Buried
108 G M -0.9848 Soluble
109 S M -0.8497 Soluble
114 S M -0.4663 Soluble
115 P M -0.5197 Soluble
116 W M -0.1115 Soluble
117 T M 0.0000 Buried
118 F M 0.0398 Aggregation-prone
119 G M 0.0000 Buried
120 Q M -1.5614 Soluble
121 G M -1.1004 Soluble
122 T M 0.0000 Buried
123 K M -0.7788 Soluble
124 V M 0.0000 Buried
125 E M 0.0000 Buried
126 I M -0.5970 Soluble
127 K M -1.2010 Soluble
128 R M -0.9236 Soluble
129 T M 0.1279 Aggregation-prone
130 V M 0.8557 Aggregation-prone
131 A M 0.2170 Aggregation-prone
132 A M -0.0635 Soluble
133 P M 0.0000 Buried
134 S M -0.1827 Soluble
135 V M 0.0000 Buried
136 F M 0.1628 Aggregation-prone
137 I M 0.1129 Aggregation-prone
138 F M 0.0000 Buried
139 P M -0.7943 Soluble
140 P M 0.0000 Buried
141 S M 0.0000 Buried
142 D M -2.5261 Soluble
143 E M -2.1513 Soluble
144 Q M 0.0000 Buried
145 L M -1.7814 Soluble
146 K M -2.6482 Soluble
147 S M -1.7025 Soluble
148 G M -1.2608 Soluble
149 T M -1.0277 Soluble
150 A M 0.0000 Buried
151 S M 0.0000 Buried
152 V M 0.0000 Buried
153 V M 0.0000 Buried
154 C M 0.0000 Buried
155 L M 0.0000 Buried
156 L M 0.0000 Buried
157 N M -0.3849 Soluble
158 N M -0.9009 Soluble
159 F M 0.0000 Buried
160 Y M 0.0000 Buried
161 P M -1.3272 Soluble
162 R M -2.3817 Soluble
163 E M -2.8806 Soluble
164 A M -2.0752 Soluble
165 K M -2.1926 Soluble
166 V M -1.1815 Soluble
167 Q M -0.5071 Soluble
168 W M 0.0000 Buried
169 K M -0.5148 Soluble
170 V M 0.0000 Buried
171 D M -1.6828 Soluble
172 N M -1.4429 Soluble
173 A M -0.2017 Soluble
174 L M 0.7543 Aggregation-prone
175 Q M -0.2748 Soluble
176 S M -0.5252 Soluble
177 G M -1.1294 Soluble
178 N M -1.2630 Soluble
179 S M -1.4737 Soluble
180 Q M -1.7015 Soluble
181 E M -2.0764 Soluble
182 S M -1.1289 Soluble
183 V M -0.8923 Soluble
184 T M -1.1485 Soluble
185 E M -2.1681 Soluble
186 Q M -1.7224 Soluble
187 D M -1.7428 Soluble
188 S M -1.7027 Soluble
189 K M -2.2197 Soluble
190 D M -1.7243 Soluble
191 S M -1.6527 Soluble
192 T M 0.0000 Buried
193 Y M 0.0000 Buried
194 S M 0.0000 Buried
195 L M 0.0000 Buried
196 S M 0.0000 Buried
197 S M 0.0000 Buried
198 T M -0.7581 Soluble
199 L M 0.0000 Buried
200 T M -0.4851 Soluble
201 L M -0.9182 Soluble
202 S M -1.3721 Soluble
203 K M -2.2357 Soluble
204 A M -2.1308 Soluble
205 D M -3.0861 Soluble
206 Y M 0.0000 Buried
207 E M -4.0608 Soluble
208 K M -3.8005 Soluble
209 H M -3.4658 Soluble
210 K M -3.3746 Soluble
211 V M -1.5055 Soluble
212 Y M 0.0000 Buried
213 A M 0.0000 Buried
214 C M 0.0000 Buried
215 E M -0.8395 Soluble
216 V M 0.0000 Buried
217 T M -1.0837 Soluble
218 H M 0.0000 Buried
219 Q M -1.5700 Soluble
220 G M -0.3387 Soluble
221 L M -0.2275 Soluble
222 S M -0.4665 Soluble
223 S M -0.3742 Soluble
224 P M -0.5534 Soluble
225 V M 0.0506 Aggregation-prone
226 T M -0.4548 Soluble
227 K M -0.7980 Soluble
228 S M -0.5885 Soluble
229 F M -1.1155 Soluble
230 N M -2.3834 Soluble
231 R M -3.0088 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6811 3.9588 CSV PDB
4.5 -0.7283 3.9433 CSV PDB
5.0 -0.7848 3.9299 CSV PDB
5.5 -0.8399 3.9222 CSV PDB
6.0 -0.8819 3.9261 CSV PDB
6.5 -0.9014 3.9418 CSV PDB
7.0 -0.8982 3.958 CSV PDB
7.5 -0.8808 3.9643 CSV PDB
8.0 -0.856 3.9629 CSV PDB
8.5 -0.8255 3.9603 CSV PDB
9.0 -0.7885 3.9589 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH