Ramucirumab / 3s34

A4DAB217_3s34_H_L | Source PDB: 3s34 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6323
Max Score
2.2928
Min Score
-3.5745
Total Score
-269.98
Score CDR
-0.2989
Score non-CDR
-0.6696

Calculation Parameters

Chain Sequence(s) EVQLVQSGGGLVKPGGSLRLSCAASGFTFSSYSMNWVRQAPGKGLEWVSSISSSSSYIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARVTDAFDIWGQGTMVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDIQMTQSPSSVSASIGDRVTITCRASQGIDNWLGWYQQKPGKAPKLLIYDASNLDTGVPSRFSGSGSGTYFTLTISSLQAEDFAVYFCQQAKAFPPTFGGGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5745
Maximum Score 2.2928
Average Score -0.6323
Total Score -269.982

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.8465 Soluble
2 V H -0.7592 Soluble
3 Q H -0.7873 Soluble
4 L H 0.0000 Buried
5 V H 1.2076 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.4190 Soluble
8 G H -0.8770 Soluble
9 G H -0.3111 Soluble
11 G H 0.6047 Aggregation-prone
12 L H 1.0727 Aggregation-prone
13 V H -0.3274 Soluble
14 K H -1.9078 Soluble
15 P H -1.9480 Soluble
16 G H -1.6137 Soluble
17 G H -1.2262 Soluble
18 S H -1.3520 Soluble
19 L H -1.0853 Soluble
20 R H -2.1401 Soluble
21 L H 0.0000 Buried
22 S H -0.4145 Soluble
23 C H 0.0000 Buried
24 A H -0.1270 Soluble
25 A H 0.0000 Buried
26 S H -0.6383 Soluble
27 G H -0.9182 Soluble
28 F H -0.3089 Soluble
29 T H -0.1734 Soluble
30 F H 0.0000 Buried
35 S H -0.7918 Soluble
36 S H -0.2292 Soluble
37 Y H 0.1124 Aggregation-prone
38 S H 0.3065 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7973 Soluble
45 A H -1.1509 Soluble
46 P H -0.8596 Soluble
47 G H -1.5462 Soluble
48 K H -2.3018 Soluble
49 G H -1.4666 Soluble
50 L H 0.0000 Buried
51 E H -1.1890 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 S H 0.0000 Buried
56 I H 0.0000 Buried
57 S H 0.6766 Aggregation-prone
58 S H -0.1302 Soluble
59 S H -0.3902 Soluble
62 S H 0.1695 Aggregation-prone
63 S H 0.6173 Aggregation-prone
64 Y H 1.9445 Aggregation-prone
65 I H 2.2928 Aggregation-prone
66 Y H 1.5660 Aggregation-prone
67 Y H 0.0379 Aggregation-prone
68 A H 0.0000 Buried
69 D H -2.2726 Soluble
70 S H -1.7487 Soluble
71 V H 0.0000 Buried
72 K H -2.3484 Soluble
74 G H -1.6460 Soluble
75 R H -1.2314 Soluble
76 F H 0.0000 Buried
77 T H -0.6020 Soluble
78 I H 0.0000 Buried
79 S H -0.2667 Soluble
80 R H 0.0000 Buried
81 D H -2.0786 Soluble
82 N H -1.8610 Soluble
83 A H -1.5291 Soluble
84 K H -2.4814 Soluble
85 N H -1.9580 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6749 Soluble
89 L H 0.0000 Buried
90 Q H -1.2961 Soluble
91 M H 0.0000 Buried
92 N H -1.3840 Soluble
93 S H -1.3302 Soluble
94 L H 0.0000 Buried
95 R H -2.5151 Soluble
96 A H -1.8687 Soluble
97 E H -2.2692 Soluble
98 D H 0.0000 Buried
99 T H 0.0000 Buried
100 A H 0.0000 Buried
101 V H 0.3221 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.2171 Soluble
107 V H -0.2655 Soluble
108 T H -0.7571 Soluble
109 D H -1.8428 Soluble
114 A H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.7672 Soluble
117 I H -0.3817 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.3012 Soluble
121 G H -0.5816 Soluble
122 T H 0.0000 Buried
123 M H 0.6836 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H 0.0000 Buried
128 S H -0.7964 Soluble
129 A H -0.5193 Soluble
130 S H -0.6985 Soluble
131 T H -0.7622 Soluble
132 K H -1.2718 Soluble
133 G H -1.4374 Soluble
134 P H 0.0000 Buried
135 S H -0.2716 Soluble
136 V H -0.4743 Soluble
137 F H 0.0000 Buried
138 P H -0.8982 Soluble
139 L H 0.0000 Buried
140 A H -1.0358 Soluble
141 P H 0.0000 Buried
142 S H -0.8048 Soluble
143 G H -0.6468 Soluble
144 T H -0.4715 Soluble
145 A H -0.2718 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.4928 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.7129 Soluble
157 E H -1.1409 Soluble
158 P H -1.0373 Soluble
159 V H -0.8306 Soluble
160 T H -0.6199 Soluble
161 V H -0.3327 Soluble
162 S H -0.4132 Soluble
163 W H 0.0000 Buried
164 N H -0.8958 Soluble
165 S H -0.7455 Soluble
166 G H -0.6208 Soluble
167 A H -0.2914 Soluble
168 L H -0.0342 Soluble
169 T H -0.1719 Soluble
170 S H -0.3330 Soluble
171 G H -0.1518 Soluble
172 V H 0.2253 Aggregation-prone
173 H H 0.0000 Buried
174 T H 0.0500 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.4659 Soluble
177 A H -0.1080 Soluble
178 V H -0.0140 Soluble
179 L H 0.1308 Aggregation-prone
180 Q H -0.1597 Soluble
181 S H -0.2185 Soluble
182 S H -0.3194 Soluble
183 G H 0.0000 Buried
184 L H -0.1994 Soluble
185 Y H -0.0590 Soluble
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1663 Soluble
193 V H 0.0000 Buried
194 P H -0.4933 Soluble
195 S H -0.5610 Soluble
196 S H -0.6508 Soluble
197 S H 0.0000 Buried
198 L H -0.8159 Soluble
199 G H -0.9553 Soluble
200 T H -0.8687 Soluble
201 Q H -1.2899 Soluble
202 T H -1.1705 Soluble
203 Y H 0.0000 Buried
204 I H -1.1499 Soluble
205 C H 0.0000 Buried
206 N H -1.4255 Soluble
207 V H 0.0000 Buried
208 N H -1.9941 Soluble
209 H H 0.0000 Buried
210 K H -2.8578 Soluble
211 P H -1.7948 Soluble
212 S H -1.8754 Soluble
213 N H -2.6084 Soluble
214 T H -2.0671 Soluble
215 K H -2.6418 Soluble
216 V H -1.5731 Soluble
217 D H -2.4262 Soluble
218 K H -1.9943 Soluble
219 R H -2.1879 Soluble
220 V H 0.0000 Buried
221 E H -2.2787 Soluble
222 P H -1.8228 Soluble
223 K H -1.9173 Soluble
224 S H -0.7509 Soluble
225 C H -0.3389 Soluble
1 D L -1.8177 Soluble
2 I L -1.5377 Soluble
3 Q L -1.9907 Soluble
4 M L 0.0000 Buried
5 T L -0.8885 Soluble
6 Q L -0.6605 Soluble
7 S L -0.5827 Soluble
8 P L -0.3852 Soluble
9 S L -0.6726 Soluble
10 S L -0.4691 Soluble
11 V L -0.0264 Soluble
12 S L -0.5879 Soluble
13 A L 0.0000 Buried
14 S L -0.0940 Soluble
15 I L 0.9028 Aggregation-prone
16 G L -0.4716 Soluble
17 D L -1.3907 Soluble
18 R L -2.1614 Soluble
19 V L 0.0000 Buried
20 T L -0.6024 Soluble
21 I L 0.0000 Buried
22 T L -0.1715 Soluble
23 C L 0.0000 Buried
24 R L -0.8968 Soluble
25 A L 0.0000 Buried
26 S L -1.4785 Soluble
27 Q L -2.1215 Soluble
28 G L -1.6040 Soluble
29 I L 0.0000 Buried
36 D L -2.4281 Soluble
37 N L -1.7206 Soluble
38 W L -1.1642 Soluble
39 L L 0.0000 Buried
40 G L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.1444 Soluble
45 K L -1.7023 Soluble
46 P L -1.5552 Soluble
47 G L -1.6935 Soluble
48 K L -2.6793 Soluble
49 A L -1.7576 Soluble
50 P L 0.0000 Buried
51 K L -1.6928 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.6323 Soluble
56 D L -1.3795 Soluble
57 A L 0.0000 Buried
65 S L -0.7858 Soluble
66 N L -0.7269 Soluble
67 L L -0.2243 Soluble
68 D L -0.5961 Soluble
69 T L -0.4709 Soluble
70 G L -0.5606 Soluble
71 V L 0.0000 Buried
72 P L -0.3910 Soluble
74 S L -0.4700 Soluble
75 R L -0.8340 Soluble
76 F L 0.0000 Buried
77 S L -0.4784 Soluble
78 G L 0.0000 Buried
79 S L -0.5877 Soluble
80 G L -0.5906 Soluble
83 S L -1.0172 Soluble
84 G L -1.1958 Soluble
85 T L -0.7220 Soluble
86 Y L 0.2648 Aggregation-prone
87 F L 0.0000 Buried
88 T L -0.1295 Soluble
89 L L 0.0000 Buried
90 T L -0.6382 Soluble
91 I L 0.0000 Buried
92 S L -1.3877 Soluble
93 S L -1.0567 Soluble
94 L L 0.0000 Buried
95 Q L -0.6775 Soluble
96 A L -0.3662 Soluble
97 E L -1.5173 Soluble
98 D L 0.0000 Buried
99 F L 0.0785 Aggregation-prone
100 A L 0.0000 Buried
101 V L 0.0585 Aggregation-prone
102 Y L 0.0000 Buried
103 F L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 A L -0.6126 Soluble
108 K L -1.3417 Soluble
109 A L 0.1778 Aggregation-prone
114 F L 1.4699 Aggregation-prone
115 P L 0.3546 Aggregation-prone
116 P L -0.0530 Soluble
117 T L -0.5912 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.1216 Soluble
121 G L -0.6310 Soluble
122 T L 0.0000 Buried
123 K L -0.1874 Soluble
124 V L 0.0000 Buried
125 D L 0.0000 Buried
126 I L 0.1408 Aggregation-prone
127 K L -1.1379 Soluble
128 R L -1.0626 Soluble
129 T L -0.2753 Soluble
130 V L 0.1967 Aggregation-prone
131 A L -0.0442 Soluble
132 A L -0.1661 Soluble
133 P L 0.0000 Buried
134 S L -0.1410 Soluble
135 V L 0.0000 Buried
136 F L 0.1777 Aggregation-prone
137 I L 0.2001 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.3658 Soluble
140 P L -0.7354 Soluble
141 S L -1.4965 Soluble
142 D L -2.7413 Soluble
143 E L -2.5382 Soluble
144 Q L 0.0000 Buried
145 L L -2.0756 Soluble
146 K L -2.6901 Soluble
147 S L -1.6509 Soluble
148 G L -1.1650 Soluble
149 T L -1.1649 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7226 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.0163 Soluble
162 R L -1.8017 Soluble
163 E L -1.9258 Soluble
164 A L -1.6331 Soluble
165 K L -1.9136 Soluble
166 V L -0.9494 Soluble
167 Q L -0.4151 Soluble
168 W L 0.0000 Buried
169 K L -0.4925 Soluble
170 V L 0.0000 Buried
171 D L -1.9551 Soluble
172 N L -1.5485 Soluble
173 A L -0.2300 Soluble
174 L L 0.7760 Aggregation-prone
175 Q L -0.2480 Soluble
176 S L -0.7203 Soluble
177 G L -1.3622 Soluble
178 N L -1.7621 Soluble
179 S L -1.5677 Soluble
180 Q L -1.7997 Soluble
181 E L -1.7514 Soluble
182 S L -0.7955 Soluble
183 V L -0.6396 Soluble
184 T L -1.0098 Soluble
185 E L -2.0546 Soluble
186 Q L -1.5653 Soluble
187 D L -1.7414 Soluble
188 S L -1.5693 Soluble
189 K L -2.2084 Soluble
190 D L -1.5524 Soluble
191 S L -1.5247 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7794 Soluble
199 L L 0.0000 Buried
200 T L -0.7097 Soluble
201 L L -0.8439 Soluble
202 S L -1.0429 Soluble
203 K L -2.0095 Soluble
204 A L -1.8845 Soluble
205 D L -2.5691 Soluble
206 Y L 0.0000 Buried
207 E L -3.3762 Soluble
208 K L -3.5745 Soluble
209 H L -3.0697 Soluble
210 K L -3.2270 Soluble
211 V L -1.4245 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6325 Soluble
216 V L 0.0000 Buried
217 T L -1.0583 Soluble
218 H L 0.0000 Buried
219 Q L -1.3571 Soluble
220 G L -0.4553 Soluble
221 L L -0.2469 Soluble
222 S L -0.4642 Soluble
223 S L -0.4181 Soluble
224 P L -0.5990 Soluble
225 V L 0.0097 Aggregation-prone
226 T L -0.4169 Soluble
227 K L -0.6659 Soluble
228 S L -0.4754 Soluble
229 F L -0.7137 Soluble
230 N L -1.7303 Soluble
231 R L -2.3340 Soluble
232 G L -1.7596 Soluble
233 E L -1.7555 Soluble
234 C L -0.2500 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5849 4.5343 CSV PDB
4.5 -0.6275 4.496 CSV PDB
5.0 -0.6768 4.4398 CSV PDB
5.5 -0.7228 4.3783 CSV PDB
6.0 -0.7547 4.3265 CSV PDB
6.5 -0.7643 4.2932 CSV PDB
7.0 -0.7521 4.2752 CSV PDB
7.5 -0.7255 4.265 CSV PDB
8.0 -0.6909 4.2583 CSV PDB
8.5 -0.6499 4.2547 CSV PDB
9.0 -0.6022 4.2569 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH