Diridavumab / 4evn

A4DAB087_4evn_O_P | Source PDB: 4evn | Chains: O, P | Done
96.55
Yes
10 Å
Yes
Avg A4D Score
-0.6327
Max Score
3.14
Min Score
-3.1265
Total Score
-274.59
Score CDR
-0.0274
Score non-CDR
-0.7169

Calculation Parameters

Chain Sequence(s) QVQLVESGAEVKKPGSSVKVSCKASGGPFRSYAISWVRQAPGQGPEWMGGIIPIFGTTKYAPKFQGRVTITADDFAGTVYMELSSLRSEDTAMYYCAKHMGYQVRETMDVWGKGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPVLTQPPSVSAAPGQKVTISCSGSSSNIGNNYVSWYQQLPGTAPKLLIYDNNKRPSGIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSSLSAYVVFGGGTKLTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE input.pdb
Selected Chain(s) O (Heavy), P (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.1265
Maximum Score 3.14
Average Score -0.6327
Total Score -274.5876

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q O -1.3831 Soluble
2 V O -0.8316 Soluble
3 Q O -1.2735 Soluble
4 L O 0.0000 Buried
5 V O 0.4742 Aggregation-prone
6 E O -0.1614 Soluble
7 S O -0.5109 Soluble
8 G O -0.5137 Soluble
9 A O -0.3688 Soluble
11 E O -0.7184 Soluble
12 V O -0.1916 Soluble
13 K O -1.4314 Soluble
14 K O -2.4125 Soluble
15 P O -2.3477 Soluble
16 G O -1.6565 Soluble
17 S O -1.3068 Soluble
18 S O -1.2324 Soluble
19 V O 0.0000 Buried
20 K O -2.0864 Soluble
21 V O 0.0000 Buried
22 S O -0.6275 Soluble
23 C O 0.0000 Buried
24 K O -0.9625 Soluble
25 A O 0.0000 Buried
26 S O -0.7480 Soluble
27 G O -0.8901 Soluble
28 G O -0.4768 Soluble
29 P O 0.3353 Aggregation-prone
30 F O 1.9188 Aggregation-prone
35 R O 0.0000 Buried
36 S O 0.9170 Aggregation-prone
37 Y O 1.0209 Aggregation-prone
38 A O 0.0000 Buried
39 I O 0.0000 Buried
40 S O 0.0000 Buried
41 W O 0.0000 Buried
42 V O 0.0000 Buried
43 R O -0.3717 Soluble
44 Q O -0.5760 Soluble
45 A O -0.9096 Soluble
46 P O -0.7478 Soluble
47 G O -1.0843 Soluble
48 Q O -1.3630 Soluble
49 G O -0.9446 Soluble
50 P O 0.0000 Buried
51 E O -0.6229 Soluble
52 W O 0.0000 Buried
53 M O 0.0000 Buried
54 G O 0.0000 Buried
55 G O 0.0000 Buried
56 I O 0.0000 Buried
57 I O 0.0000 Buried
58 P O 0.0000 Buried
59 I O 3.1400 Aggregation-prone
62 F O 2.7531 Aggregation-prone
63 G O 1.1049 Aggregation-prone
64 T O 0.5432 Aggregation-prone
65 T O -0.1731 Soluble
66 K O -0.7572 Soluble
67 Y O -0.8418 Soluble
68 A O 0.0000 Buried
69 P O -1.3122 Soluble
70 K O -2.2608 Soluble
71 F O 0.0000 Buried
72 Q O -2.1740 Soluble
74 G O -1.5598 Soluble
75 R O -1.5303 Soluble
76 V O 0.0000 Buried
77 T O -0.9235 Soluble
78 I O 0.0000 Buried
79 T O -0.4097 Soluble
80 A O -0.1860 Soluble
81 D O -0.3411 Soluble
82 D O 0.5068 Aggregation-prone
83 F O 1.7208 Aggregation-prone
84 A O 0.5022 Aggregation-prone
85 G O 0.0000 Buried
86 T O -0.2125 Soluble
87 V O 0.0000 Buried
88 Y O -0.6744 Soluble
89 M O 0.0000 Buried
90 E O -1.5385 Soluble
91 L O 0.0000 Buried
92 S O -1.2342 Soluble
93 S O -1.2703 Soluble
94 L O 0.0000 Buried
95 R O -3.1265 Soluble
96 S O -2.4096 Soluble
97 E O -2.5477 Soluble
98 D O 0.0000 Buried
99 T O -0.9940 Soluble
100 A O 0.0000 Buried
101 M O -0.0729 Soluble
102 Y O 0.0000 Buried
103 Y O 0.0000 Buried
104 C O 0.0000 Buried
105 A O 0.0000 Buried
106 K O -0.0077 Soluble
107 H O 0.0000 Buried
108 M O 0.2294 Aggregation-prone
109 G O 0.2243 Aggregation-prone
110 Y O 1.0991 Aggregation-prone
111 Q O -0.3431 Soluble
112A V O 0.0000 Buried
112 R O -1.8967 Soluble
113 E O -1.4001 Soluble
114 T O -0.6846 Soluble
115 M O 0.0000 Buried
116 D O -0.3607 Soluble
117 V O -0.1857 Soluble
118 W O 0.0000 Buried
119 G O 0.0000 Buried
120 K O -1.5000 Soluble
121 G O -0.7590 Soluble
122 T O 0.0000 Buried
123 T O -0.1072 Soluble
124 V O 0.0000 Buried
125 T O -0.7706 Soluble
126 V O 0.0000 Buried
127 S O -1.2927 Soluble
128 S O -1.1384 Soluble
129 A O -0.7278 Soluble
130 S O -0.7572 Soluble
131 T O -0.6925 Soluble
132 K O -1.2860 Soluble
133 G O -1.3532 Soluble
134 P O -0.5449 Soluble
135 S O -0.2132 Soluble
136 V O 0.0000 Buried
137 F O 0.0000 Buried
138 P O -1.0266 Soluble
139 L O 0.0000 Buried
140 A O -0.9184 Soluble
141 P O 0.0000 Buried
142 S O -1.0829 Soluble
143 S O -1.2657 Soluble
144 K O -2.0135 Soluble
145 S O -1.1371 Soluble
146 T O -1.0814 Soluble
147 S O -0.9943 Soluble
148 G O -0.8834 Soluble
149 G O -0.9104 Soluble
150 T O -0.6281 Soluble
151 A O 0.0000 Buried
152 A O -0.3304 Soluble
153 L O 0.0000 Buried
154 G O 0.0000 Buried
155 C O 0.0000 Buried
156 L O 0.0000 Buried
157 V O 0.0000 Buried
158 K O 0.0000 Buried
159 D O -0.1907 Soluble
160 Y O 0.0000 Buried
161 F O 0.0000 Buried
162 P O 0.0000 Buried
163 E O -0.8160 Soluble
164 P O -0.8742 Soluble
165 V O -0.6896 Soluble
166 T O -0.6095 Soluble
167 V O -0.3334 Soluble
168 S O -0.3116 Soluble
169 W O 0.0000 Buried
170 N O -0.7556 Soluble
171 S O -0.6220 Soluble
172 G O -0.4735 Soluble
173 A O -0.1884 Soluble
174 L O 0.0980 Aggregation-prone
175 T O -0.1261 Soluble
176 S O -0.2512 Soluble
177 G O -0.3173 Soluble
178 V O 0.1248 Aggregation-prone
179 H O -0.2080 Soluble
180 T O -0.0576 Soluble
181 F O 0.0000 Buried
182 P O -0.2600 Soluble
183 A O 0.0504 Aggregation-prone
184 V O 0.0000 Buried
185 L O 1.2052 Aggregation-prone
186 Q O 0.2730 Aggregation-prone
187 S O -0.1266 Soluble
188 S O -0.1642 Soluble
189 G O 0.0437 Aggregation-prone
190 L O 0.1362 Aggregation-prone
191 Y O 0.3665 Aggregation-prone
192 S O 0.0000 Buried
193 L O 0.0000 Buried
194 S O 0.0000 Buried
195 S O 0.0000 Buried
196 V O 0.0000 Buried
197 V O 0.0000 Buried
198 T O -0.2030 Soluble
199 V O 0.0000 Buried
200 P O -0.6356 Soluble
201 S O -0.6655 Soluble
202 S O -0.5995 Soluble
203 S O -0.6499 Soluble
204 L O -0.8569 Soluble
205 G O -1.0919 Soluble
206 T O -0.7189 Soluble
207 Q O -1.2722 Soluble
208 T O -1.1365 Soluble
209 Y O 0.0000 Buried
210 I O -1.2438 Soluble
211 C O 0.0000 Buried
212 N O -1.4333 Soluble
213 V O 0.0000 Buried
214 N O -1.8374 Soluble
215 H O 0.0000 Buried
216 K O -2.7712 Soluble
217 P O -1.7374 Soluble
218 S O -1.8415 Soluble
219 N O -2.5783 Soluble
220 T O -2.0590 Soluble
221 K O -2.5873 Soluble
222 V O -1.4069 Soluble
223 D O -2.3718 Soluble
224 K O -2.1134 Soluble
225 R O -2.7150 Soluble
226 V O 0.0000 Buried
227 E O -2.5710 Soluble
228 P O -1.2644 Soluble
3 V P 1.6172 Aggregation-prone
4 L P 0.0000 Buried
5 T P 0.0611 Aggregation-prone
6 Q P 0.0000 Buried
7 P P -0.5205 Soluble
8 P P -0.7553 Soluble
9 S P -0.7285 Soluble
11 V P -0.2654 Soluble
12 S P -0.2471 Soluble
13 A P -0.4813 Soluble
14 A P -0.7553 Soluble
15 P P -1.3645 Soluble
16 G P -1.8963 Soluble
17 Q P -2.5397 Soluble
18 K P -2.5635 Soluble
19 V P 0.0000 Buried
20 T P -0.4908 Soluble
21 I P 0.0000 Buried
22 S P -0.3034 Soluble
23 C P 0.0000 Buried
24 S P -0.1546 Soluble
25 G P -0.0527 Soluble
26 S P -0.3957 Soluble
27 S P -0.9018 Soluble
28 S P -0.5652 Soluble
29 N P 0.0000 Buried
30 I P 0.0000 Buried
35 G P -1.0695 Soluble
36 N P -1.3452 Soluble
37 N P -0.7421 Soluble
38 Y P 0.0158 Aggregation-prone
39 V P 0.0000 Buried
40 S P 0.0000 Buried
41 W P 0.0000 Buried
42 Y P 0.0000 Buried
43 Q P 0.0000 Buried
44 Q P -0.6204 Soluble
45 L P -0.3074 Soluble
46 P P -0.5281 Soluble
47 G P -0.7872 Soluble
48 T P -0.8549 Soluble
49 A P -0.9646 Soluble
50 P P 0.0000 Buried
51 K P -0.8973 Soluble
52 L P 0.0000 Buried
53 L P 0.0000 Buried
54 I P 0.0000 Buried
55 Y P -1.2580 Soluble
56 D P -1.6441 Soluble
57 N P -1.5662 Soluble
65 N P -2.5731 Soluble
66 K P -2.8708 Soluble
67 R P -2.2767 Soluble
68 P P -1.1029 Soluble
69 S P -0.9174 Soluble
70 G P -0.8097 Soluble
71 I P -0.8641 Soluble
72 P P -1.3046 Soluble
74 D P -2.2458 Soluble
75 R P -1.6169 Soluble
76 F P 0.0000 Buried
77 S P -1.5361 Soluble
78 G P -1.4820 Soluble
79 S P -1.3601 Soluble
80 K P -1.1057 Soluble
83 S P -0.8132 Soluble
84 G P -1.0217 Soluble
85 T P -0.8852 Soluble
86 S P -0.6546 Soluble
87 A P 0.0000 Buried
88 T P -0.5650 Soluble
89 L P 0.0000 Buried
90 G P -0.6492 Soluble
91 I P 0.0000 Buried
92 T P -1.9072 Soluble
93 G P -1.7031 Soluble
94 L P 0.0000 Buried
95 Q P -1.7220 Soluble
96 T P -1.0358 Soluble
97 G P -0.9713 Soluble
98 D P 0.0000 Buried
99 E P -0.8450 Soluble
100 A P 0.0000 Buried
101 D P -0.9878 Soluble
102 Y P 0.0000 Buried
103 Y P 0.0000 Buried
104 C P 0.0000 Buried
105 G P 0.0000 Buried
106 T P 0.0000 Buried
107 W P 0.0000 Buried
108 D P 0.0000 Buried
109 S P -0.4030 Soluble
110 S P 0.5216 Aggregation-prone
112 L P 1.4121 Aggregation-prone
113 S P 0.4347 Aggregation-prone
114 A P 0.1686 Aggregation-prone
115 Y P -0.0604 Soluble
116 V P 0.0000 Buried
117 V P 0.0000 Buried
118 F P 0.0000 Buried
119 G P 0.0000 Buried
120 G P -0.6752 Soluble
121 G P -0.9571 Soluble
122 T P 0.0000 Buried
123 K P -1.4144 Soluble
124 L P 0.0000 Buried
125 T P -0.3150 Soluble
126 V P 0.0000 Buried
127 L P 0.3798 Aggregation-prone
128 G P -0.5557 Soluble
129 Q P -1.1069 Soluble
130 P P -1.4413 Soluble
131 K P -2.5936 Soluble
132 A P -1.9380 Soluble
133 N P -2.0935 Soluble
134 P P 0.0000 Buried
135 T P -0.4477 Soluble
136 V P 0.0000 Buried
137 T P -0.5702 Soluble
138 L P 0.0000 Buried
139 F P 0.0000 Buried
140 P P -0.1593 Soluble
141 P P -0.4689 Soluble
142 S P -0.8014 Soluble
143 S P -1.3653 Soluble
144 E P -2.1305 Soluble
145 E P 0.0000 Buried
146 L P -1.8258 Soluble
147 Q P -2.2277 Soluble
148 A P -1.7565 Soluble
149 N P -2.4738 Soluble
150 K P -2.1912 Soluble
151 A P 0.0000 Buried
152 T P 0.0000 Buried
153 L P 0.0000 Buried
154 V P 0.0000 Buried
155 C P 0.0000 Buried
156 L P 0.0000 Buried
157 I P 0.0000 Buried
158 S P -0.6352 Soluble
159 D P -1.3388 Soluble
160 F P 0.0000 Buried
161 Y P -1.5036 Soluble
162 P P -1.1589 Soluble
163 G P -0.9056 Soluble
164 A P -0.4106 Soluble
165 V P -0.3004 Soluble
166 T P -0.0700 Soluble
167 V P 0.1611 Aggregation-prone
168 A P -0.3859 Soluble
169 W P 0.0000 Buried
170 K P -1.1806 Soluble
171 A P -1.5745 Soluble
172 D P -2.1866 Soluble
173 G P -1.4724 Soluble
174 S P -0.9199 Soluble
175 P P -1.0155 Soluble
176 V P -0.8823 Soluble
177 K P -1.6352 Soluble
178 A P -0.8257 Soluble
179 G P -0.6593 Soluble
180 V P -0.4523 Soluble
181 E P -0.4605 Soluble
182 T P -0.3865 Soluble
183 T P -0.7921 Soluble
184 K P -1.7493 Soluble
185 P P -1.0667 Soluble
186 S P -1.0409 Soluble
187 K P -1.4085 Soluble
188 Q P -1.4277 Soluble
189 S P -1.3747 Soluble
190 N P -2.0795 Soluble
191 N P -1.9586 Soluble
192 K P -1.8463 Soluble
193 Y P -0.9247 Soluble
194 A P 0.0000 Buried
195 A P 0.0000 Buried
196 S P 0.0000 Buried
197 S P 0.0000 Buried
198 Y P 0.0000 Buried
199 L P 0.0000 Buried
200 S P -0.5401 Soluble
201 L P 0.0000 Buried
202 T P -1.7408 Soluble
203 P P -2.3627 Soluble
204 E P -3.0770 Soluble
205 Q P -2.3450 Soluble
206 W P 0.0000 Buried
207 K P -3.0365 Soluble
208 S P -2.5073 Soluble
209 H P -2.5243 Soluble
210 R P -3.0090 Soluble
211 S P 0.0000 Buried
212 Y P 0.0000 Buried
213 S P 0.0000 Buried
214 C P 0.0000 Buried
215 Q P -0.7528 Soluble
216 V P 0.0000 Buried
217 T P -0.7613 Soluble
218 H P 0.0000 Buried
219 E P -2.4934 Soluble
220 G P -1.4013 Soluble
221 S P -1.0777 Soluble
222 T P -0.8234 Soluble
223 V P -0.6117 Soluble
224 E P -1.3376 Soluble
225 K P -1.3660 Soluble
226 T P -1.0218 Soluble
227 V P 0.0000 Buried
228 A P -1.6062 Soluble
229 P P -1.6648 Soluble
230 T P -1.2190 Soluble
231 E P -2.1402 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5776 6.2206 CSV PDB
4.5 -0.608 6.1383 CSV PDB
5.0 -0.6426 6.0502 CSV PDB
5.5 -0.6726 5.9602 CSV PDB
6.0 -0.6882 5.8696 CSV PDB
6.5 -0.6814 5.7791 CSV PDB
7.0 -0.652 5.6896 CSV PDB
7.5 -0.608 5.637 CSV PDB
8.0 -0.5567 5.637 CSV PDB
8.5 -0.501 5.637 CSV PDB
9.0 -0.4415 5.637 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH