Valanafusp / 5kqv

A4DAB287_5kqv_P_Q | Source PDB: 5kqv | Chains: P, Q | Done
99.55
Yes
10 Å
Yes
Avg A4D Score
-0.7295
Max Score
1.1455
Min Score
-3.6711
Total Score
-316.61
Score CDR
-0.6489
Score non-CDR
-0.7377

Calculation Parameters

Chain Sequence(s) QVQLQQSGPELVKPGALVKISCKASGYTFTNYDIHWVKQRPGQGLEWIGWIYPGDGSTKYNEKFKGKATLTADKSSSTAYMHLSSLTSEKSAVYFCAREWAYWGQGTLVTVSAAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIKPCDIQMTQSPSSLSASLGERVSLTCRASQDIGGNLYWLQQGPDGTIKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISSLESEDFVDYYCLQYSSSPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRGEC input.pdb
Selected Chain(s) P (Heavy), Q (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6711
Maximum Score 1.1455
Average Score -0.7295
Total Score -316.6147

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q P -1.4066 Soluble
2 V P -0.8190 Soluble
3 Q P -1.6587 Soluble
4 L P 0.0000 Buried
5 Q P -2.0127 Soluble
6 Q P 0.0000 Buried
7 S P -0.8023 Soluble
8 G P -0.2250 Soluble
9 P P 0.0699 Aggregation-prone
11 E P 0.1070 Aggregation-prone
12 L P 0.8179 Aggregation-prone
13 V P 0.0455 Aggregation-prone
14 K P -1.2780 Soluble
15 P P -1.0372 Soluble
16 G P -0.6926 Soluble
17 A P -0.0378 Soluble
18 L P 0.6166 Aggregation-prone
19 V P 0.0000 Buried
20 K P -1.1665 Soluble
21 I P 0.0000 Buried
22 S P -0.8183 Soluble
23 C P 0.0000 Buried
24 K P -1.8720 Soluble
25 A P 0.0000 Buried
26 S P -1.1861 Soluble
27 G P -0.9538 Soluble
28 Y P -0.4645 Soluble
29 T P -0.3646 Soluble
30 F P 0.0000 Buried
35 T P -1.0908 Soluble
36 N P -1.1289 Soluble
37 Y P -0.5387 Soluble
38 D P -0.5213 Soluble
39 I P 0.0000 Buried
40 H P 0.0000 Buried
41 W P 0.0000 Buried
42 V P 0.0000 Buried
43 K P -0.7674 Soluble
44 Q P -1.1253 Soluble
45 R P -1.7629 Soluble
46 P P -1.3864 Soluble
47 G P -1.3875 Soluble
48 Q P -2.0302 Soluble
49 G P -1.4036 Soluble
50 L P 0.0000 Buried
51 E P -1.4904 Soluble
52 W P 0.0000 Buried
53 I P 0.0000 Buried
54 G P 0.0000 Buried
55 W P -0.1697 Soluble
56 I P 0.0000 Buried
57 Y P -0.4837 Soluble
58 P P 0.0000 Buried
59 G P -1.4941 Soluble
62 D P -1.9660 Soluble
63 G P -1.4644 Soluble
64 S P -0.9353 Soluble
65 T P -0.5679 Soluble
66 K P -1.0251 Soluble
67 Y P -1.6847 Soluble
68 N P -2.3006 Soluble
69 E P -3.6202 Soluble
70 K P -3.5318 Soluble
71 F P 0.0000 Buried
72 K P -3.6006 Soluble
74 G P -2.6409 Soluble
75 K P -2.7292 Soluble
76 A P 0.0000 Buried
77 T P -0.9732 Soluble
78 L P 0.0000 Buried
79 T P -0.4254 Soluble
80 A P -1.0352 Soluble
81 D P -1.5527 Soluble
82 K P -1.8238 Soluble
83 S P -1.1080 Soluble
84 S P -1.1151 Soluble
85 S P -1.2327 Soluble
86 T P 0.0000 Buried
87 A P 0.0000 Buried
88 Y P -0.4656 Soluble
89 M P 0.0000 Buried
90 H P -0.6643 Soluble
91 L P 0.0000 Buried
92 S P -0.4827 Soluble
93 S P -0.6154 Soluble
94 L P 0.0000 Buried
95 T P -1.0184 Soluble
96 S P -1.0574 Soluble
97 E P -1.3467 Soluble
98 K P -2.0780 Soluble
99 S P -0.8690 Soluble
100 A P 0.0000 Buried
101 V P 0.0496 Aggregation-prone
102 Y P 0.0000 Buried
103 F P 0.0000 Buried
104 C P 0.0000 Buried
105 A P 0.0000 Buried
106 R P -0.5064 Soluble
107 E P -0.7790 Soluble
115 W P 0.0000 Buried
116 A P -0.1711 Soluble
117 Y P 0.1673 Aggregation-prone
118 W P 0.0000 Buried
119 G P 0.0000 Buried
120 Q P -1.2003 Soluble
121 G P -0.5960 Soluble
122 T P 0.0000 Buried
123 L P 0.7487 Aggregation-prone
124 V P 0.0000 Buried
125 T P -0.2228 Soluble
126 V P 0.0000 Buried
127 S P -0.9796 Soluble
128 A P -0.8398 Soluble
129 A P -1.0856 Soluble
130 K P -1.7574 Soluble
131 T P -0.9428 Soluble
132 T P -0.4870 Soluble
133 A P -0.4579 Soluble
134 P P 0.0000 Buried
135 S P -0.2070 Soluble
136 V P 0.0000 Buried
137 Y P 0.0000 Buried
138 P P 0.0000 Buried
139 L P 0.0000 Buried
140 A P 0.0000 Buried
141 P P -0.0993 Soluble
142 V P 0.3707 Aggregation-prone
143 C P -0.9512 Soluble
144 G P -1.7901 Soluble
145 D P -1.7707 Soluble
146 T P -0.9303 Soluble
147 T P -0.6765 Soluble
148 G P -0.6652 Soluble
149 S P -0.4010 Soluble
150 S P -0.2452 Soluble
151 V P 0.0014 Aggregation-prone
152 T P 0.1802 Aggregation-prone
153 L P 0.0000 Buried
154 G P 0.0000 Buried
155 C P 0.0000 Buried
156 L P 0.0000 Buried
157 V P 0.0000 Buried
158 K P -0.2810 Soluble
159 G P -0.3318 Soluble
160 Y P 0.0000 Buried
161 F P 0.0000 Buried
162 P P -0.5073 Soluble
163 E P -0.7477 Soluble
164 P P -0.6148 Soluble
165 V P -0.3278 Soluble
166 T P -0.2692 Soluble
167 L P -0.0951 Soluble
168 T P -0.3473 Soluble
169 W P 0.0000 Buried
170 N P -1.0162 Soluble
171 S P -0.7930 Soluble
172 G P -0.7768 Soluble
173 S P -0.5811 Soluble
174 L P -0.2300 Soluble
175 S P -0.4114 Soluble
176 S P -0.4890 Soluble
177 G P -0.4021 Soluble
178 V P 0.0901 Aggregation-prone
179 H P -0.3037 Soluble
180 T P -0.1213 Soluble
181 F P 0.0000 Buried
182 P P -0.2247 Soluble
183 A P 0.0389 Aggregation-prone
184 V P 0.2362 Aggregation-prone
185 L P -0.1921 Soluble
186 Q P -0.8074 Soluble
187 S P -0.7256 Soluble
188 D P -1.0882 Soluble
189 L P -0.4508 Soluble
190 Y P -0.1314 Soluble
191 T P -0.0720 Soluble
192 L P 0.0000 Buried
193 S P 0.0000 Buried
194 S P 0.0000 Buried
195 S P 0.0000 Buried
196 V P 0.0000 Buried
197 T P 0.0579 Aggregation-prone
198 V P 0.0938 Aggregation-prone
199 T P -0.1716 Soluble
200 S P -0.3775 Soluble
201 S P -0.5654 Soluble
202 T P -0.6376 Soluble
203 W P 0.0000 Buried
204 P P -1.1286 Soluble
205 S P -1.1990 Soluble
206 Q P -1.5043 Soluble
207 S P -1.5482 Soluble
208 I P 0.0000 Buried
209 T P -1.6896 Soluble
210 C P 0.0000 Buried
211 N P -1.2759 Soluble
212 V P 0.0000 Buried
213 A P -0.7814 Soluble
214 H P 0.0000 Buried
215 P P -0.6820 Soluble
216 A P -0.3383 Soluble
217 S P -0.4624 Soluble
218 S P -0.6690 Soluble
219 T P -0.8612 Soluble
220 K P -1.8730 Soluble
221 V P -1.2035 Soluble
222 D P -1.9539 Soluble
223 K P -2.1944 Soluble
224 K P -2.6274 Soluble
225 I P -1.9072 Soluble
226 E P -2.4395 Soluble
227 P P -1.0508 Soluble
228 R P -1.0810 Soluble
229 G P -1.4153 Soluble
230 P P -0.1020 Soluble
231 T P 0.1628 Aggregation-prone
232 I P 1.1455 Aggregation-prone
233 K P -0.7327 Soluble
234 P P -0.2180 Soluble
235 C P 0.4597 Aggregation-prone
1 D Q -2.3293 Soluble
2 I Q 0.0000 Buried
3 Q Q -2.2820 Soluble
4 M Q 0.0000 Buried
5 T Q -1.3193 Soluble
6 Q Q -1.0681 Soluble
7 S Q -0.7702 Soluble
8 P Q -0.5278 Soluble
9 S Q -0.6923 Soluble
10 S Q -0.5605 Soluble
11 L Q -0.1780 Soluble
12 S Q -0.3968 Soluble
13 A Q 0.0000 Buried
14 S Q -0.5003 Soluble
15 L Q -0.0581 Soluble
16 G Q -0.9521 Soluble
17 E Q -1.6659 Soluble
18 R Q -2.2223 Soluble
19 V Q -1.0836 Soluble
20 S Q -0.5472 Soluble
21 L Q 0.0000 Buried
22 T Q -0.9161 Soluble
23 C Q 0.0000 Buried
24 R Q -2.8867 Soluble
25 A Q 0.0000 Buried
26 S Q -2.2898 Soluble
27 Q Q -2.9237 Soluble
28 D Q -2.8518 Soluble
29 I Q 0.0000 Buried
36 G Q -1.2181 Soluble
37 G Q -0.8462 Soluble
38 N Q -0.6649 Soluble
39 L Q 0.0000 Buried
40 Y Q 0.0000 Buried
41 W Q 0.0000 Buried
42 L Q 0.0000 Buried
43 Q Q 0.0000 Buried
44 Q Q 0.0000 Buried
45 G Q -1.3340 Soluble
46 P Q -1.3579 Soluble
47 D Q -2.0879 Soluble
48 G Q -1.4108 Soluble
49 T Q -1.2998 Soluble
50 I Q 0.0000 Buried
51 K Q -1.6018 Soluble
52 R Q -0.7369 Soluble
53 L Q 0.0000 Buried
54 I Q 0.0000 Buried
55 Y Q 0.4028 Aggregation-prone
56 A Q 0.0367 Aggregation-prone
57 T Q 0.0000 Buried
65 S Q -0.4082 Soluble
66 S Q 0.0561 Aggregation-prone
67 L Q -0.1963 Soluble
68 D Q -0.6331 Soluble
69 S Q -0.5279 Soluble
70 G Q -0.8376 Soluble
71 V Q 0.0000 Buried
72 P Q -1.1760 Soluble
74 K Q -1.9722 Soluble
75 R Q -1.5740 Soluble
76 F Q 0.0000 Buried
77 S Q -0.6987 Soluble
78 G Q -0.4210 Soluble
79 S Q -0.9411 Soluble
80 R Q -1.6673 Soluble
83 S Q -1.2793 Soluble
84 G Q -1.4270 Soluble
85 S Q -1.9631 Soluble
86 D Q -1.6914 Soluble
87 Y Q 0.0000 Buried
88 S Q -0.8836 Soluble
89 L Q 0.0000 Buried
90 T Q -0.6280 Soluble
91 I Q 0.0000 Buried
92 S Q -1.6086 Soluble
93 S Q -1.4564 Soluble
94 L Q 0.0000 Buried
95 E Q -1.4897 Soluble
96 S Q -1.1642 Soluble
97 E Q -1.9988 Soluble
98 D Q 0.0000 Buried
99 F Q -0.4120 Soluble
100 V Q -0.4262 Soluble
101 D Q -0.4887 Soluble
102 Y Q 0.0000 Buried
103 Y Q 0.0000 Buried
104 C Q 0.0000 Buried
105 L Q 0.0000 Buried
106 Q Q 0.0000 Buried
107 Y Q 0.0927 Aggregation-prone
108 S Q -0.6268 Soluble
109 S Q -0.7865 Soluble
114 S Q -0.5722 Soluble
115 P Q -0.8657 Soluble
116 W Q -0.3846 Soluble
117 T Q -0.6717 Soluble
118 F Q -0.3065 Soluble
119 G Q 0.0000 Buried
120 G Q -1.0380 Soluble
121 G Q 0.0000 Buried
122 T Q 0.0000 Buried
123 K Q -0.7482 Soluble
124 L Q 0.0000 Buried
125 E Q 0.0000 Buried
126 I Q -0.0826 Soluble
127 K Q -1.2197 Soluble
128 R Q -1.5432 Soluble
129 A Q -1.3699 Soluble
130 D Q -2.4197 Soluble
131 A Q -1.3525 Soluble
132 A Q -0.6514 Soluble
133 P Q 0.0000 Buried
134 T Q -0.0065 Soluble
135 V Q 0.1883 Aggregation-prone
136 S Q 0.2202 Aggregation-prone
137 I Q 0.0000 Buried
138 F Q 0.0000 Buried
139 P Q 0.0000 Buried
140 P Q 0.0000 Buried
141 S Q 0.0000 Buried
142 S Q -0.7191 Soluble
143 E Q -1.3618 Soluble
144 Q Q 0.0000 Buried
145 L Q -1.0817 Soluble
146 T Q -0.7825 Soluble
147 S Q -0.8137 Soluble
148 G Q -1.1989 Soluble
149 G Q -1.0842 Soluble
150 A Q 0.0000 Buried
151 S Q 0.0000 Buried
152 V Q 0.0000 Buried
153 V Q 0.0000 Buried
154 C Q 0.0000 Buried
155 F Q 0.0000 Buried
156 L Q 0.0000 Buried
157 N Q 0.0000 Buried
158 N Q -0.6632 Soluble
159 F Q 0.0000 Buried
160 Y Q 0.0000 Buried
161 P Q -2.1643 Soluble
162 K Q -2.4581 Soluble
163 D Q -2.7684 Soluble
164 I Q -1.7865 Soluble
165 N Q -1.8441 Soluble
166 V Q -0.7199 Soluble
167 K Q -1.5845 Soluble
168 W Q 0.0000 Buried
169 K Q -2.2943 Soluble
170 I Q 0.0000 Buried
171 D Q -3.1542 Soluble
172 G Q -2.1055 Soluble
173 S Q -2.1297 Soluble
174 E Q -2.9848 Soluble
175 R Q -2.5530 Soluble
176 Q Q -2.4572 Soluble
177 N Q -2.0508 Soluble
178 G Q -1.0093 Soluble
179 V Q -0.5907 Soluble
180 L Q -0.1907 Soluble
181 N Q 0.0542 Aggregation-prone
182 S Q 0.3088 Aggregation-prone
183 W Q 0.1873 Aggregation-prone
184 T Q -0.7900 Soluble
185 D Q -2.0072 Soluble
186 Q Q -1.6008 Soluble
187 D Q -1.6950 Soluble
188 S Q -1.5456 Soluble
189 K Q -2.1481 Soluble
190 D Q -1.4210 Soluble
191 S Q -1.4372 Soluble
192 T Q 0.0000 Buried
193 Y Q 0.0000 Buried
194 S Q 0.0000 Buried
195 M Q 0.0000 Buried
196 S Q 0.0000 Buried
197 S Q 0.0000 Buried
198 T Q 0.0401 Aggregation-prone
199 L Q 0.0000 Buried
200 T Q -0.2977 Soluble
201 L Q 0.0000 Buried
202 T Q -1.4086 Soluble
203 K Q -2.4222 Soluble
204 D Q -3.2304 Soluble
205 E Q -2.4996 Soluble
206 Y Q 0.0000 Buried
207 E Q -3.3179 Soluble
208 R Q -3.6711 Soluble
209 H Q -3.1841 Soluble
210 N Q -3.2544 Soluble
211 S Q -2.3099 Soluble
212 Y Q 0.0000 Buried
213 T Q -1.1453 Soluble
214 C Q 0.0000 Buried
215 E Q -0.4272 Soluble
216 A Q 0.0000 Buried
217 T Q -1.0051 Soluble
218 H Q 0.0000 Buried
219 K Q -2.5913 Soluble
220 T Q -1.3455 Soluble
221 S Q -0.6881 Soluble
222 T Q -0.5381 Soluble
223 S Q -0.3136 Soluble
224 P Q -0.2890 Soluble
225 I Q 0.7016 Aggregation-prone
226 V Q 0.7949 Aggregation-prone
227 K Q 0.0319 Aggregation-prone
228 S Q -0.3971 Soluble
229 F Q 0.0000 Buried
230 N Q -2.6015 Soluble
231 R Q -2.7273 Soluble
232 G Q -2.2505 Soluble
233 E Q -2.3838 Soluble
234 C Q -1.1998 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7063 2.6626 CSV PDB
4.5 -0.7541 2.5785 CSV PDB
5.0 -0.8075 2.5097 CSV PDB
5.5 -0.8572 2.5033 CSV PDB
6.0 -0.892 2.64 CSV PDB
6.5 -0.9032 2.8508 CSV PDB
7.0 -0.8914 3.0789 CSV PDB
7.5 -0.8645 3.3127 CSV PDB
8.0 -0.8286 3.5475 CSV PDB
8.5 -0.7847 3.7797 CSV PDB
9.0 -0.7322 4.0022 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH