Nesfrotamig / 5tdp

A4DAB178_5tdp_D_C | Source PDB: 5tdp | Chains: D, C | Done
98.68
Yes
10 Å
Yes
Avg A4D Score
-0.6842
Max Score
1.342
Min Score
-3.6455
Total Score
-291.48
Score CDR
-0.5283
Score non-CDR
-0.7035

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLQPGGSLRLSCAASGFNIKDTYIHWVREAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSGTAILGCLVKDYFPEPVTVSWNSGALTSGVHTSPAVLQSSGLYMLASAVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQKKPGKAPKLLIYSASFLESGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVAIFPPSDEQLKSGTASVVCVLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYALFSVLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6455
Maximum Score 1.342
Average Score -0.6842
Total Score -291.4754

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.1913 Soluble
2 V D -1.5437 Soluble
3 Q D -1.3366 Soluble
4 L D 0.0000 Buried
5 V D 1.1568 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.2908 Soluble
8 G D -0.8756 Soluble
9 G D -0.3348 Soluble
11 G D 0.1887 Aggregation-prone
12 L D 0.4572 Aggregation-prone
14 Q D -1.4933 Soluble
15 P D -1.5683 Soluble
16 G D -1.3733 Soluble
17 G D -1.0738 Soluble
18 S D -1.1006 Soluble
19 L D -1.0492 Soluble
20 R D -2.1510 Soluble
21 L D 0.0000 Buried
22 S D -0.3830 Soluble
23 C D 0.0000 Buried
24 A D 0.0570 Aggregation-prone
25 A D 0.0000 Buried
26 S D -1.0891 Soluble
27 G D -1.5752 Soluble
28 F D -1.6453 Soluble
29 N D -2.5773 Soluble
30 I D 0.0000 Buried
35 K D -2.9575 Soluble
36 D D -3.1203 Soluble
37 T D 0.0000 Buried
38 Y D -0.6346 Soluble
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 E D -0.8793 Soluble
45 A D 0.0000 Buried
46 P D -1.2565 Soluble
47 G D -1.6249 Soluble
48 K D -2.6434 Soluble
49 G D -1.6639 Soluble
50 L D 0.0000 Buried
51 E D -1.2734 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 R D -0.6216 Soluble
56 I D 0.0000 Buried
57 Y D -0.4054 Soluble
58 P D -1.1082 Soluble
59 T D -1.2457 Soluble
62 N D -0.9678 Soluble
63 G D -0.3351 Soluble
64 Y D 0.5172 Aggregation-prone
65 T D -0.4403 Soluble
66 R D -2.0275 Soluble
67 Y D -1.9051 Soluble
68 A D -1.8663 Soluble
69 D D -2.6480 Soluble
70 S D -1.7120 Soluble
71 V D 0.0000 Buried
72 K D -2.8265 Soluble
74 G D -1.6853 Soluble
75 R D -1.2526 Soluble
76 F D 0.0000 Buried
77 T D -1.1847 Soluble
78 I D 0.0000 Buried
79 S D -0.1371 Soluble
80 A D -0.7237 Soluble
81 D D -1.3667 Soluble
82 T D -1.9086 Soluble
83 S D -1.6068 Soluble
84 K D -2.2116 Soluble
85 N D -1.9080 Soluble
86 T D -0.9900 Soluble
87 A D 0.0000 Buried
88 Y D -0.6067 Soluble
89 L D 0.0000 Buried
90 Q D -1.4853 Soluble
91 M D 0.0000 Buried
92 N D -1.2557 Soluble
93 S D -1.1213 Soluble
94 L D 0.0000 Buried
95 R D -2.1173 Soluble
96 A D -1.5999 Soluble
97 E D -2.3159 Soluble
98 D D 0.0000 Buried
99 T D -0.6109 Soluble
100 A D 0.0000 Buried
101 V D 0.2660 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 S D 0.0000 Buried
106 R D -0.5186 Soluble
107 W D 0.0000 Buried
108 G D 0.0000 Buried
109 G D -0.9844 Soluble
110 D D -1.8186 Soluble
111 G D 0.0000 Buried
112 F D 0.8023 Aggregation-prone
113 Y D 0.6699 Aggregation-prone
114 A D 0.0000 Buried
115 M D 0.0000 Buried
116 D D -0.0806 Soluble
117 Y D -0.2144 Soluble
118 W D -0.3746 Soluble
119 G D 0.0000 Buried
120 Q D -1.2226 Soluble
121 G D 0.0000 Buried
122 T D 0.1594 Aggregation-prone
123 L D 0.4359 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.1947 Soluble
126 V D 0.0000 Buried
127 S D -0.6613 Soluble
128 S D -0.6560 Soluble
129 A D -0.4404 Soluble
130 S D -0.5961 Soluble
131 T D -0.7908 Soluble
132 K D -1.4430 Soluble
133 G D -1.5413 Soluble
134 P D 0.0000 Buried
135 S D -0.4079 Soluble
136 V D 0.0000 Buried
137 F D -0.5374 Soluble
138 P D -0.9459 Soluble
139 L D 0.0000 Buried
140 A D -0.7535 Soluble
141 P D 0.0000 Buried
142 S D -0.2950 Soluble
143 G D -0.6443 Soluble
144 T D -0.3978 Soluble
145 A D -0.1495 Soluble
146 I D 0.1754 Aggregation-prone
147 L D 0.0000 Buried
148 G D 0.0000 Buried
149 C D 0.0000 Buried
150 L D 0.0000 Buried
151 V D 0.0000 Buried
152 K D 0.0000 Buried
153 D D -0.4981 Soluble
154 Y D 0.0000 Buried
155 F D 0.0000 Buried
156 P D 0.0000 Buried
157 E D -0.5021 Soluble
158 P D -0.7800 Soluble
159 V D -0.7460 Soluble
160 T D -0.6143 Soluble
161 V D -0.1690 Soluble
162 S D -0.4007 Soluble
163 W D 0.0000 Buried
164 N D -0.7447 Soluble
165 S D -0.6836 Soluble
166 G D -0.5210 Soluble
167 A D -0.2336 Soluble
168 L D 0.0204 Aggregation-prone
169 T D -0.1612 Soluble
170 S D -0.1634 Soluble
171 G D -0.2193 Soluble
172 V D 0.2012 Aggregation-prone
173 H D -0.4464 Soluble
174 T D -0.1937 Soluble
175 S D 0.0000 Buried
176 P D -0.2048 Soluble
177 A D 0.3134 Aggregation-prone
178 V D 0.7327 Aggregation-prone
179 L D 1.3273 Aggregation-prone
180 Q D 0.3845 Aggregation-prone
181 S D -0.0997 Soluble
182 S D -0.1783 Soluble
183 G D 0.1144 Aggregation-prone
184 L D 0.1274 Aggregation-prone
185 Y D 0.4471 Aggregation-prone
186 M D 0.0000 Buried
187 L D 0.0000 Buried
188 A D 0.0000 Buried
189 S D 0.0000 Buried
190 A D 0.0000 Buried
191 V D 0.0000 Buried
192 T D -0.0445 Soluble
193 V D 0.0000 Buried
194 P D -0.5464 Soluble
195 S D -0.4735 Soluble
196 S D -0.5571 Soluble
197 S D -0.5338 Soluble
198 L D -0.7193 Soluble
199 G D -1.0809 Soluble
200 T D -0.8772 Soluble
201 Q D -1.2135 Soluble
202 T D -1.1014 Soluble
203 Y D 0.0000 Buried
204 I D -1.2985 Soluble
205 C D 0.0000 Buried
206 N D -1.5072 Soluble
207 V D 0.0000 Buried
208 N D -2.0371 Soluble
209 H D 0.0000 Buried
210 K D -2.7911 Soluble
211 P D -1.5824 Soluble
212 S D -1.9251 Soluble
213 N D -2.6314 Soluble
214 T D -2.1687 Soluble
215 K D -2.7794 Soluble
216 V D -1.6552 Soluble
217 D D -2.5331 Soluble
218 K D -2.0537 Soluble
219 K D -2.3417 Soluble
220 V D 0.0000 Buried
221 E D -2.4782 Soluble
222 P D -1.2211 Soluble
2 I C -0.5243 Soluble
3 Q C -1.6073 Soluble
4 M C 0.0000 Buried
5 T C -1.4066 Soluble
6 Q C 0.0000 Buried
7 S C -0.9337 Soluble
8 P C -0.6997 Soluble
9 S C -1.0029 Soluble
10 S C -0.9903 Soluble
11 L C -0.6576 Soluble
12 S C -0.7980 Soluble
13 A C -0.8512 Soluble
14 S C -0.6770 Soluble
15 V C 0.1995 Aggregation-prone
16 G C -0.6469 Soluble
17 D C -1.6818 Soluble
18 R C -2.2302 Soluble
19 V C 0.0000 Buried
20 T C -0.6051 Soluble
21 I C 0.0000 Buried
22 T C -0.7879 Soluble
23 C C 0.0000 Buried
24 R C -2.8025 Soluble
25 A C 0.0000 Buried
26 S C -1.8092 Soluble
27 Q C -2.3476 Soluble
28 D C -2.7295 Soluble
29 V C 0.0000 Buried
36 N C -1.8593 Soluble
37 T C -0.8198 Soluble
38 A C 0.0640 Aggregation-prone
39 V C 0.0000 Buried
40 A C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 K C 0.0000 Buried
45 K C -1.4961 Soluble
46 P C -1.3237 Soluble
47 G C -1.6221 Soluble
48 K C -2.5428 Soluble
49 A C -1.5921 Soluble
50 P C 0.0000 Buried
51 K C -1.4062 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C 0.0000 Buried
56 S C 0.4507 Aggregation-prone
57 A C 0.0000 Buried
65 S C 0.2701 Aggregation-prone
66 F C 1.3420 Aggregation-prone
67 L C 0.8221 Aggregation-prone
68 E C 0.0593 Aggregation-prone
69 S C -0.2203 Soluble
70 G C -0.5049 Soluble
71 V C -0.3445 Soluble
72 P C -0.3300 Soluble
74 S C -0.4088 Soluble
75 R C -0.6720 Soluble
76 F C 0.0000 Buried
77 S C -0.0292 Soluble
78 G C -0.0899 Soluble
79 S C -0.6710 Soluble
80 R C -1.5855 Soluble
83 S C -1.1921 Soluble
84 G C -1.7996 Soluble
85 T C -2.1931 Soluble
86 D C -2.0296 Soluble
87 F C 0.0000 Buried
88 T C -0.7705 Soluble
89 L C 0.0000 Buried
90 T C -0.6019 Soluble
91 I C 0.0000 Buried
92 S C -1.2875 Soluble
93 S C -1.0383 Soluble
94 L C 0.0000 Buried
95 Q C -0.5402 Soluble
96 P C -1.2654 Soluble
97 E C -1.6036 Soluble
98 D C 0.0000 Buried
99 F C -0.9660 Soluble
100 A C 0.0000 Buried
101 T C -0.9577 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.5582 Aggregation-prone
108 Y C 0.6262 Aggregation-prone
109 T C 0.5837 Aggregation-prone
114 T C -0.1395 Soluble
115 P C -0.5331 Soluble
116 P C 0.0000 Buried
117 T C -0.3144 Soluble
118 F C -0.4805 Soluble
119 G C 0.0000 Buried
120 Q C -1.9729 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -1.8672 Soluble
124 V C 0.0000 Buried
125 E C -1.2733 Soluble
126 I C 0.0000 Buried
127 K C -1.1387 Soluble
128 R C -0.7538 Soluble
129 T C -0.1397 Soluble
130 V C 0.2243 Aggregation-prone
131 A C -0.1313 Soluble
132 A C -0.3197 Soluble
133 P C 0.0000 Buried
134 S C -0.2620 Soluble
135 V C -0.0634 Soluble
136 A C -0.0103 Soluble
137 I C 0.0495 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5188 Soluble
140 P C -0.7051 Soluble
141 S C -1.4144 Soluble
142 D C -2.6741 Soluble
143 E C -2.3516 Soluble
144 Q C 0.0000 Buried
145 L C -2.0561 Soluble
146 K C -2.6475 Soluble
147 S C -1.6394 Soluble
148 G C -1.3542 Soluble
149 T C -1.1852 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 V C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -1.3650 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.3701 Soluble
162 R C -1.9628 Soluble
163 E C -2.7379 Soluble
164 A C -2.1754 Soluble
165 K C -2.4824 Soluble
166 V C 0.0000 Buried
167 Q C -0.9199 Soluble
168 W C 0.0000 Buried
169 K C -0.7835 Soluble
170 V C 0.0000 Buried
171 D C -1.9193 Soluble
172 N C -1.6347 Soluble
173 A C -0.4801 Soluble
174 L C 0.2788 Aggregation-prone
175 Q C -0.4061 Soluble
176 S C -0.5642 Soluble
177 G C -0.9033 Soluble
178 N C -0.8650 Soluble
179 S C -0.9336 Soluble
180 Q C -0.9145 Soluble
181 E C -1.0982 Soluble
182 S C 0.0000 Buried
183 V C -0.3919 Soluble
184 T C 0.0000 Buried
185 E C -1.8523 Soluble
186 Q C 0.0000 Buried
187 D C -2.1940 Soluble
188 S C -2.1321 Soluble
189 K C -2.5202 Soluble
190 D C -1.9966 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 A C 0.0000 Buried
195 L C 0.0000 Buried
196 F C 0.0000 Buried
197 S C 0.0000 Buried
198 V C 0.0000 Buried
199 L C 0.0000 Buried
200 T C -0.4468 Soluble
201 L C -0.7926 Soluble
202 S C -1.1559 Soluble
203 K C -2.1749 Soluble
204 A C -2.0194 Soluble
205 D C -2.8047 Soluble
206 Y C 0.0000 Buried
207 E C -3.6453 Soluble
208 K C -3.6455 Soluble
209 H C -2.8855 Soluble
210 K C -2.5617 Soluble
211 V C -1.1135 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9259 Soluble
216 V C 0.0000 Buried
217 T C -1.3070 Soluble
218 H C 0.0000 Buried
219 Q C -1.6899 Soluble
220 G C -0.5337 Soluble
221 L C -0.3018 Soluble
222 S C -0.4736 Soluble
223 S C -0.4291 Soluble
224 P C -0.5694 Soluble
225 V C 0.0187 Aggregation-prone
226 T C -0.5458 Soluble
227 K C -0.8093 Soluble
228 S C -0.6372 Soluble
229 F C -0.7178 Soluble
230 N C -1.5828 Soluble
231 R C -2.1226 Soluble
232 G C -1.8146 Soluble
233 E C -2.0590 Soluble
234 C C -0.4254 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6809 3.0555 CSV PDB
4.5 -0.7259 2.9636 CSV PDB
5.0 -0.7778 2.8495 CSV PDB
5.5 -0.8273 2.7263 CSV PDB
6.0 -0.8635 2.6001 CSV PDB
6.5 -0.8771 2.4721 CSV PDB
7.0 -0.8677 2.3428 CSV PDB
7.5 -0.8432 2.2141 CSV PDB
8.0 -0.8105 2.1623 CSV PDB
8.5 -0.7713 2.1618 CSV PDB
9.0 -0.7249 2.1614 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH