Tazlestobart / 6jc2

A4DAB258_6jc2_H_L | Source PDB: 6jc2 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6931
Max Score
2.0781
Min Score
-3.7404
Total Score
-295.24
Score CDR
-0.1845
Score non-CDR
-0.7546

Calculation Parameters

Chain Sequence(s) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7404
Maximum Score 2.0781
Average Score -0.6931
Total Score -295.2401

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.5173 Soluble
2 V H -1.0100 Soluble
3 Q H -1.1732 Soluble
4 L H 0.0000 Buried
5 V H 0.5781 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.2528 Soluble
8 G H -0.5911 Soluble
9 G H 0.2369 Aggregation-prone
11 G H 0.6592 Aggregation-prone
12 V H 1.4012 Aggregation-prone
13 V H -0.2892 Soluble
14 Q H -1.5353 Soluble
15 P H -2.1071 Soluble
16 G H -2.2803 Soluble
17 R H -2.8270 Soluble
18 S H -1.9990 Soluble
19 L H -1.3384 Soluble
20 R H -2.0224 Soluble
21 L H 0.0000 Buried
22 S H -0.2999 Soluble
23 C H 0.0000 Buried
24 A H -0.1535 Soluble
25 A H 0.0000 Buried
26 S H -0.9360 Soluble
27 G H -1.0335 Soluble
28 F H -0.4044 Soluble
29 T H -0.2021 Soluble
30 F H 0.0000 Buried
35 S H -0.5292 Soluble
36 S H 0.0456 Aggregation-prone
37 Y H 0.3471 Aggregation-prone
38 T H 0.0000 Buried
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.5554 Soluble
45 A H -0.9736 Soluble
46 P H -0.8021 Soluble
47 G H -1.4677 Soluble
48 K H -2.3881 Soluble
49 G H -1.6199 Soluble
50 L H 0.0000 Buried
51 E H -1.0218 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 T H 0.0000 Buried
55 F H 0.2099 Aggregation-prone
56 I H 0.0000 Buried
57 S H -0.7631 Soluble
58 Y H -0.6012 Soluble
59 D H -2.1362 Soluble
62 G H -1.8961 Soluble
63 N H -2.2973 Soluble
64 N H -1.7219 Soluble
65 K H -0.8492 Soluble
66 Y H 0.1043 Aggregation-prone
67 Y H -0.5827 Soluble
68 A H -1.1699 Soluble
69 D H -2.2881 Soluble
70 S H -1.6231 Soluble
71 V H 0.0000 Buried
72 K H -2.3881 Soluble
74 G H -1.7398 Soluble
75 R H -1.4427 Soluble
76 F H 0.0000 Buried
77 T H -0.7333 Soluble
78 I H 0.0000 Buried
79 S H -0.5549 Soluble
80 R H -0.9732 Soluble
81 D H -1.5834 Soluble
82 N H -1.8050 Soluble
83 S H -1.6006 Soluble
84 K H -2.3735 Soluble
85 N H -1.7445 Soluble
86 T H -0.9390 Soluble
87 L H 0.0000 Buried
88 Y H -0.2387 Soluble
89 L H 0.0000 Buried
90 Q H -1.1024 Soluble
91 M H 0.0000 Buried
92 N H -2.0834 Soluble
93 S H -1.9498 Soluble
94 L H 0.0000 Buried
95 R H -2.8364 Soluble
96 A H -1.8172 Soluble
97 E H -2.3216 Soluble
98 D H 0.0000 Buried
99 T H -0.3468 Soluble
100 A H 0.0000 Buried
101 I H 0.9903 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 T H 0.4771 Aggregation-prone
108 G H 0.9763 Aggregation-prone
109 W H 1.8549 Aggregation-prone
110 L H 2.0781 Aggregation-prone
113 G H 0.9622 Aggregation-prone
114 P H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.0798 Soluble
117 Y H -0.2536 Soluble
118 W H -0.2748 Soluble
119 G H 0.0000 Buried
120 Q H -1.0938 Soluble
121 G H -0.0974 Soluble
122 T H 0.6299 Aggregation-prone
123 L H 1.7956 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.3157 Aggregation-prone
126 V H 0.0000 Buried
127 S H -0.6904 Soluble
128 S H -0.6849 Soluble
129 A H -0.4696 Soluble
130 S H -0.6290 Soluble
131 T H -0.9865 Soluble
132 K H -1.7762 Soluble
133 G H -1.7539 Soluble
134 P H -0.8896 Soluble
135 S H -0.6336 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2427 Soluble
139 L H 0.0000 Buried
140 A H -0.8115 Soluble
141 P H 0.0000 Buried
142 S H -0.3936 Soluble
143 G H -0.6365 Soluble
144 T H -0.4622 Soluble
145 A H -0.2481 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H -0.3856 Soluble
153 D H -0.6274 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.7502 Soluble
157 E H -1.0091 Soluble
158 P H -0.9935 Soluble
159 V H -0.8632 Soluble
160 T H -0.7853 Soluble
161 V H -0.4755 Soluble
162 S H -0.4043 Soluble
163 W H 0.0000 Buried
164 N H -0.6555 Soluble
165 S H -0.6119 Soluble
166 G H -0.5239 Soluble
167 A H -0.2193 Soluble
168 L H 0.0495 Aggregation-prone
169 T H -0.1443 Soluble
170 S H -0.1648 Soluble
171 G H -0.1681 Soluble
172 V H 0.2078 Aggregation-prone
173 H H -0.0743 Soluble
174 T H 0.0337 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.2326 Soluble
177 A H 0.2814 Aggregation-prone
178 V H 0.7242 Aggregation-prone
179 L H 1.2908 Aggregation-prone
180 Q H 0.2706 Aggregation-prone
181 S H -0.0415 Soluble
182 S H -0.2099 Soluble
183 G H -0.0001 Buried
184 L H -0.1064 Soluble
185 Y H 0.2959 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.0933 Soluble
193 V H 0.0000 Buried
194 P H -0.5722 Soluble
195 S H -0.5165 Soluble
196 S H -0.6053 Soluble
197 S H -0.5917 Soluble
198 L H -0.8316 Soluble
199 G H -0.9822 Soluble
200 T H -0.6984 Soluble
201 Q H -1.1494 Soluble
202 T H -0.9629 Soluble
203 Y H 0.0000 Buried
204 I H -1.0390 Soluble
205 C H 0.0000 Buried
206 N H -1.5311 Soluble
207 V H 0.0000 Buried
208 N H -2.3457 Soluble
209 H H 0.0000 Buried
210 K H -2.8414 Soluble
211 P H -1.8119 Soluble
212 S H -2.0650 Soluble
213 N H -2.6307 Soluble
214 T H -2.2192 Soluble
215 K H -2.8005 Soluble
216 V H -1.5864 Soluble
217 D H -2.5825 Soluble
218 K H -2.1126 Soluble
219 K H -2.2848 Soluble
220 V H 0.0000 Buried
221 E H -2.4081 Soluble
222 P H -1.2178 Soluble
1 E L -1.3898 Soluble
2 I L -0.0746 Soluble
3 V L 0.7868 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.6720 Soluble
6 Q L 0.0000 Buried
7 S L -0.8875 Soluble
8 P L -0.5014 Soluble
9 G L -0.8479 Soluble
10 T L -0.4206 Soluble
11 L L -0.2480 Soluble
12 S L -0.6161 Soluble
13 L L 0.0000 Buried
14 S L -1.9020 Soluble
15 P L -2.2420 Soluble
16 G L -2.6791 Soluble
17 E L -3.1996 Soluble
18 R L -3.2740 Soluble
19 A L 0.0000 Buried
20 T L -0.5338 Soluble
21 L L 0.0000 Buried
22 S L -0.8868 Soluble
23 C L 0.0000 Buried
24 R L -2.2491 Soluble
25 A L -1.1049 Soluble
26 S L -0.8515 Soluble
27 Q L -1.6284 Soluble
28 S L -1.4231 Soluble
29 V L 0.0000 Buried
30 G L -0.7594 Soluble
36 S L -0.3806 Soluble
37 S L 0.1232 Aggregation-prone
38 Y L 0.7703 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6056 Soluble
46 P L -1.4203 Soluble
47 G L -1.4284 Soluble
48 Q L -2.0504 Soluble
49 A L -1.2838 Soluble
50 P L 0.0000 Buried
51 R L -1.2819 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.8186 Aggregation-prone
56 G L 0.8776 Aggregation-prone
57 A L 0.0000 Buried
65 F L 1.6160 Aggregation-prone
66 S L 0.4321 Aggregation-prone
67 R L -0.8701 Soluble
68 A L -0.5031 Soluble
69 T L -0.5325 Soluble
70 G L -0.7910 Soluble
71 I L -0.9234 Soluble
72 P L -1.2019 Soluble
74 D L -2.3398 Soluble
75 R L -2.0977 Soluble
76 F L 0.0000 Buried
77 S L -0.3122 Soluble
78 G L 0.4144 Aggregation-prone
79 S L 0.1077 Aggregation-prone
80 G L -0.6063 Soluble
83 S L -0.7493 Soluble
84 G L -1.2410 Soluble
85 T L -1.6850 Soluble
86 D L -1.9801 Soluble
87 F L 0.0000 Buried
88 T L -0.4688 Soluble
89 L L 0.0000 Buried
90 T L -0.7956 Soluble
91 I L 0.0000 Buried
92 S L -2.5546 Soluble
93 R L -3.7404 Soluble
94 L L 0.0000 Buried
95 E L -2.8476 Soluble
96 P L -1.9582 Soluble
97 E L -2.4625 Soluble
98 D L 0.0000 Buried
99 F L -0.7745 Soluble
100 A L 0.0000 Buried
101 V L -0.3461 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.2146 Aggregation-prone
107 Y L 0.0000 Buried
108 G L -0.2748 Soluble
109 S L -0.3918 Soluble
114 S L -0.4282 Soluble
115 P L -0.5676 Soluble
116 W L -0.0876 Soluble
117 T L -0.1210 Soluble
118 F L 0.0296 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.4129 Soluble
121 G L -1.0812 Soluble
122 T L 0.0000 Buried
123 K L -0.5112 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.7435 Soluble
127 K L -1.6794 Soluble
128 R L -1.1803 Soluble
129 T L -0.1453 Soluble
130 V L 0.5309 Aggregation-prone
131 A L 0.1149 Aggregation-prone
132 A L -0.0910 Soluble
133 P L 0.0000 Buried
134 S L -0.1327 Soluble
135 V L 0.0000 Buried
136 F L 0.0667 Aggregation-prone
137 I L 0.0885 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6657 Soluble
140 P L 0.0000 Buried
141 S L -1.7306 Soluble
142 D L -3.1599 Soluble
143 E L -3.3488 Soluble
144 Q L 0.0000 Buried
145 L L -2.4305 Soluble
146 K L -3.0254 Soluble
147 S L -1.9205 Soluble
148 G L -1.5503 Soluble
149 T L -1.2961 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7882 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.2693 Soluble
162 R L -2.0779 Soluble
163 E L -2.7722 Soluble
164 A L -2.1526 Soluble
165 K L -2.3583 Soluble
166 V L 0.0000 Buried
167 Q L -0.9560 Soluble
168 W L 0.0000 Buried
169 K L -0.7650 Soluble
170 V L 0.0000 Buried
171 D L -1.5888 Soluble
172 N L -1.3957 Soluble
173 A L -0.3794 Soluble
174 L L 0.4004 Aggregation-prone
175 Q L -0.4678 Soluble
176 S L -0.7783 Soluble
177 G L -1.3631 Soluble
178 N L -1.7515 Soluble
179 S L -1.6394 Soluble
180 Q L -1.6433 Soluble
181 E L -1.5277 Soluble
182 S L -0.6999 Soluble
183 V L -0.3576 Soluble
184 T L -1.0146 Soluble
185 E L -2.1148 Soluble
186 Q L -1.7365 Soluble
187 D L -1.9200 Soluble
188 S L -1.6907 Soluble
189 K L -2.2393 Soluble
190 D L -1.6869 Soluble
191 S L -1.7209 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7776 Soluble
199 L L 0.0000 Buried
200 T L -0.8334 Soluble
201 L L -0.9515 Soluble
202 S L -1.2101 Soluble
203 K L -2.1587 Soluble
204 A L -2.0535 Soluble
205 D L -2.8354 Soluble
206 Y L 0.0000 Buried
207 E L -3.6396 Soluble
208 K L -3.5503 Soluble
209 H L -2.7021 Soluble
210 K L -2.5723 Soluble
211 V L -1.1679 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8657 Soluble
216 V L 0.0000 Buried
217 T L -1.1813 Soluble
218 H L 0.0000 Buried
219 Q L -1.4336 Soluble
220 G L -0.3994 Soluble
221 L L -0.2017 Soluble
222 S L -0.4301 Soluble
223 S L -0.3523 Soluble
224 P L -0.5474 Soluble
225 V L 0.1075 Aggregation-prone
226 T L -0.4895 Soluble
227 K L -0.7724 Soluble
228 S L -0.6158 Soluble
229 F L -0.9186 Soluble
230 N L -1.7818 Soluble
231 R L -2.3065 Soluble
232 G L -2.0397 Soluble
233 E L -2.4437 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6575 4.3535 CSV PDB
4.5 -0.7037 4.3415 CSV PDB
5.0 -0.7578 4.3293 CSV PDB
5.5 -0.8103 4.317 CSV PDB
6.0 -0.8503 4.3048 CSV PDB
6.5 -0.8685 4.2927 CSV PDB
7.0 -0.8636 4.281 CSV PDB
7.5 -0.8429 4.2705 CSV PDB
8.0 -0.813 4.2625 CSV PDB
8.5 -0.7759 4.2578 CSV PDB
9.0 -0.7319 4.2557 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH