Paridiprubart / 4r7n

A4DAB203_4r7n_E_F | Source PDB: 4r7n | Chains: E, F | Done
96
Yes
10 Å
Yes
Avg A4D Score
-0.7177
Max Score
1.7738
Min Score
-4.0906
Total Score
-307.88
Score CDR
-0.2057
Score non-CDR
-0.7777

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSDTLSLTCAVSGYPIRFGYSWHWIRQPPGKGLEWMGYIHYSGYTDFNPSLKTRITISRDTSKNQFSLKLSSVTAVDTAVYYCARKDSGNYFPYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPEIVLTQSPDFQSVTPKEKVTITCRASQSISDHLHWYQQKPDQSPKLLIKYASHAISGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCQQGYDFPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) E (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.0906
Maximum Score 1.7738
Average Score -0.7177
Total Score -307.8827

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q E -1.3951 Soluble
2 V E -0.9108 Soluble
3 Q E -1.6963 Soluble
4 L E 0.0000 Buried
5 Q E -1.9812 Soluble
6 E E -1.2538 Soluble
7 S E -1.0572 Soluble
8 G E -0.6466 Soluble
9 P E -0.4846 Soluble
11 G E -0.4770 Soluble
12 L E -0.2688 Soluble
13 V E 0.0000 Buried
14 K E -2.0472 Soluble
15 P E -1.3273 Soluble
16 S E -1.1119 Soluble
17 D E -1.3824 Soluble
18 T E -1.0264 Soluble
19 L E 0.0000 Buried
20 S E -0.6144 Soluble
21 L E 0.0000 Buried
22 T E -0.7283 Soluble
23 C E 0.0000 Buried
24 A E -1.1764 Soluble
25 V E -0.8713 Soluble
26 S E -1.0710 Soluble
27 G E -0.8705 Soluble
28 Y E -0.1147 Soluble
29 P E -0.1856 Soluble
30 I E 0.0000 Buried
31 R E -0.8653 Soluble
35 F E 1.2795 Aggregation-prone
36 G E 0.7705 Aggregation-prone
37 Y E 0.5432 Aggregation-prone
38 S E 0.0000 Buried
39 W E 0.0000 Buried
40 H E 0.0000 Buried
41 W E 0.0000 Buried
42 I E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.7777 Soluble
45 P E -0.6455 Soluble
46 P E -1.0045 Soluble
47 G E -1.4622 Soluble
48 K E -2.2516 Soluble
49 G E -1.4073 Soluble
50 L E 0.0000 Buried
51 E E -0.8288 Soluble
52 W E 0.0000 Buried
53 M E 0.0000 Buried
54 G E 0.0000 Buried
55 Y E 0.0784 Aggregation-prone
56 I E 0.0000 Buried
57 H E 0.3383 Aggregation-prone
58 Y E 0.6195 Aggregation-prone
59 S E 0.3220 Aggregation-prone
63 G E -0.0488 Soluble
64 Y E 0.9850 Aggregation-prone
65 T E 0.2237 Aggregation-prone
66 D E -0.5684 Soluble
67 F E -0.5254 Soluble
68 N E -0.8019 Soluble
69 P E -1.0440 Soluble
70 S E -0.8120 Soluble
71 L E 0.0000 Buried
72 K E -1.8657 Soluble
74 T E -1.0655 Soluble
75 R E -1.1078 Soluble
76 I E 0.0000 Buried
77 T E -0.7785 Soluble
78 I E 0.0000 Buried
79 S E -0.5967 Soluble
80 R E -1.4725 Soluble
81 D E -1.8582 Soluble
82 T E -1.6670 Soluble
83 S E -1.6480 Soluble
84 K E -2.2470 Soluble
85 N E -1.7495 Soluble
86 Q E -1.4688 Soluble
87 F E 0.0000 Buried
88 S E -0.7010 Soluble
89 L E 0.0000 Buried
90 K E -1.0016 Soluble
91 L E 0.0000 Buried
92 S E -0.7837 Soluble
93 S E -0.8611 Soluble
94 V E 0.0000 Buried
95 T E 0.0705 Aggregation-prone
96 A E 0.6527 Aggregation-prone
97 V E 1.7738 Aggregation-prone
98 D E 0.0000 Buried
99 T E 0.6529 Aggregation-prone
100 A E 0.0000 Buried
101 V E -0.0874 Soluble
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 A E 0.0000 Buried
106 R E 0.0000 Buried
107 K E -0.2732 Soluble
108 D E -0.2568 Soluble
109 S E -0.3853 Soluble
110 G E -0.2437 Soluble
113 N E -0.3504 Soluble
114 Y E -0.1747 Soluble
115 F E 0.0000 Buried
116 P E -0.0866 Soluble
117 Y E -0.1090 Soluble
118 W E -0.6636 Soluble
119 G E 0.0000 Buried
120 Q E -2.2879 Soluble
121 G E -1.3765 Soluble
122 T E -0.4854 Soluble
123 L E 0.1271 Aggregation-prone
124 V E 0.0000 Buried
125 T E 0.0242 Aggregation-prone
126 V E 0.0000 Buried
127 S E -0.6677 Soluble
128 S E -0.8661 Soluble
129 A E -0.6635 Soluble
130 S E -0.8265 Soluble
131 T E -1.0608 Soluble
132 K E -2.0397 Soluble
133 G E -1.9500 Soluble
134 P E -0.8892 Soluble
135 S E -0.3342 Soluble
136 V E 0.0000 Buried
137 F E -0.6902 Soluble
138 P E -1.3585 Soluble
139 L E -0.9541 Soluble
140 A E -0.7005 Soluble
141 P E -0.7597 Soluble
142 S E -0.9821 Soluble
143 S E -1.4285 Soluble
144 K E -2.1699 Soluble
145 S E -1.4674 Soluble
146 T E -1.0830 Soluble
147 S E -1.1987 Soluble
148 G E -1.1678 Soluble
149 G E -1.0270 Soluble
150 T E -0.5647 Soluble
151 A E -0.5404 Soluble
152 A E 0.0000 Buried
153 L E 0.0000 Buried
154 G E 0.0000 Buried
155 C E 0.0000 Buried
156 L E 0.0000 Buried
157 V E 0.0000 Buried
158 K E 0.0000 Buried
159 D E -0.9389 Soluble
160 Y E 0.0000 Buried
161 F E 0.0000 Buried
162 P E 0.0000 Buried
163 E E -0.2759 Soluble
164 P E -0.5872 Soluble
165 V E -0.4735 Soluble
166 T E -0.4194 Soluble
167 V E -0.0542 Soluble
168 S E -0.2425 Soluble
169 W E 0.0000 Buried
170 N E -0.6376 Soluble
171 S E -0.4768 Soluble
172 G E -0.4788 Soluble
173 A E -0.2305 Soluble
174 L E -0.0683 Soluble
175 T E -0.2441 Soluble
176 S E -0.3059 Soluble
177 G E -0.4253 Soluble
178 V E -0.0320 Soluble
179 H E -0.3544 Soluble
180 T E -0.1077 Soluble
181 F E 0.0000 Buried
182 P E -0.3477 Soluble
183 A E 0.3373 Aggregation-prone
184 V E 0.6499 Aggregation-prone
185 L E 1.3448 Aggregation-prone
186 Q E 0.2169 Aggregation-prone
187 S E -0.1685 Soluble
188 S E -0.2798 Soluble
189 G E -0.0129 Soluble
190 L E -0.2023 Soluble
191 Y E 0.2821 Aggregation-prone
192 S E 0.0000 Buried
193 L E 0.0000 Buried
194 S E 0.0000 Buried
195 S E 0.0000 Buried
196 V E 0.0000 Buried
197 V E 0.0000 Buried
198 T E -0.2043 Soluble
199 V E 0.0000 Buried
200 P E -0.4561 Soluble
201 S E -0.5982 Soluble
202 S E -0.6441 Soluble
203 S E -0.5836 Soluble
204 L E -0.8027 Soluble
205 G E -1.1706 Soluble
206 T E -0.9304 Soluble
207 Q E -1.2488 Soluble
208 T E -1.2254 Soluble
209 Y E 0.0000 Buried
210 I E -0.9574 Soluble
211 C E 0.0000 Buried
212 N E -1.1097 Soluble
213 V E 0.0000 Buried
214 N E -1.9181 Soluble
215 H E 0.0000 Buried
216 K E -2.7234 Soluble
217 P E -1.7102 Soluble
218 S E -2.0296 Soluble
219 N E -2.4902 Soluble
220 T E -1.8417 Soluble
221 K E -2.4457 Soluble
222 V E -0.9686 Soluble
223 D E -2.3718 Soluble
224 K E -2.0029 Soluble
225 R E -2.7252 Soluble
226 V E 0.0000 Buried
227 E E -2.5554 Soluble
228 P E -1.2079 Soluble
1 E F -1.4536 Soluble
2 I F -0.1851 Soluble
3 V F 0.7116 Aggregation-prone
4 L F 0.0000 Buried
5 T F -0.8821 Soluble
6 Q F 0.0000 Buried
7 S F -1.0361 Soluble
8 P F -0.8176 Soluble
9 D F -1.5340 Soluble
10 F F 0.0662 Aggregation-prone
11 Q F -0.2954 Soluble
12 S F -0.3365 Soluble
13 V F -1.2657 Soluble
14 T F -1.8928 Soluble
15 P F -2.4589 Soluble
16 K F -3.4911 Soluble
17 E F -3.9156 Soluble
18 K F -3.4168 Soluble
19 V F 0.0000 Buried
20 T F -0.6858 Soluble
21 I F 0.0000 Buried
22 T F -1.0939 Soluble
23 C F 0.0000 Buried
24 R F -2.4534 Soluble
25 A F 0.0000 Buried
26 S F -0.9721 Soluble
27 Q F -1.4311 Soluble
28 S F -1.4337 Soluble
29 I F 0.0000 Buried
36 S F -1.3647 Soluble
37 D F -1.9391 Soluble
38 H F -1.0861 Soluble
39 L F 0.0000 Buried
40 H F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F 0.0000 Buried
45 K F -1.7968 Soluble
46 P F -1.7945 Soluble
47 D F -2.7644 Soluble
48 Q F -2.6333 Soluble
49 S F -1.5846 Soluble
50 P F 0.0000 Buried
51 K F -1.2921 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 K F -0.7232 Soluble
56 Y F -0.7846 Soluble
57 A F 0.0000 Buried
65 S F -1.0181 Soluble
66 H F -1.0269 Soluble
67 A F -0.4318 Soluble
68 I F -0.0470 Soluble
69 S F -0.2830 Soluble
70 G F -0.3815 Soluble
71 V F -0.2169 Soluble
72 P F -0.2867 Soluble
74 S F -0.6052 Soluble
75 R F -1.2612 Soluble
76 F F 0.0000 Buried
77 S F -0.7903 Soluble
78 G F -0.5756 Soluble
79 S F -0.9792 Soluble
80 G F -1.4874 Soluble
83 S F -1.4906 Soluble
84 G F -1.6780 Soluble
85 T F -2.0250 Soluble
86 D F -2.4583 Soluble
87 F F 0.0000 Buried
88 T F -0.8415 Soluble
89 L F 0.0000 Buried
90 T F -0.8670 Soluble
91 I F 0.0000 Buried
92 N F -2.9202 Soluble
93 S F -2.9603 Soluble
94 L F 0.0000 Buried
95 E F -2.8596 Soluble
96 A F -2.5590 Soluble
97 E F -2.8227 Soluble
98 D F 0.0000 Buried
99 A F 0.0000 Buried
100 A F 0.0000 Buried
101 T F -0.5563 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 G F 0.0000 Buried
108 Y F 0.1354 Aggregation-prone
109 D F -0.8361 Soluble
114 F F -0.1953 Soluble
115 P F -0.4216 Soluble
116 L F 0.0000 Buried
117 T F 0.0000 Buried
118 F F 0.2518 Aggregation-prone
119 G F 0.0000 Buried
120 G F -1.0735 Soluble
121 G F -1.0570 Soluble
122 T F 0.0000 Buried
123 K F -0.8768 Soluble
124 V F 0.0000 Buried
125 E F -0.6168 Soluble
126 I F 0.0000 Buried
127 K F -1.2623 Soluble
128 R F -0.9077 Soluble
129 T F -0.1687 Soluble
130 V F 0.3062 Aggregation-prone
131 A F 0.0474 Aggregation-prone
132 A F -0.1883 Soluble
133 P F 0.0000 Buried
134 S F -0.2285 Soluble
135 V F 0.0000 Buried
136 F F -0.0578 Soluble
137 I F 0.0000 Buried
138 F F 0.0000 Buried
139 P F -0.7757 Soluble
140 P F -0.7634 Soluble
141 S F -1.6713 Soluble
142 D F -3.0397 Soluble
143 E F -3.0672 Soluble
144 Q F -2.4420 Soluble
145 L F -2.3378 Soluble
146 K F -2.8553 Soluble
147 S F -1.8042 Soluble
148 G F -1.3669 Soluble
149 T F -1.0013 Soluble
150 A F 0.0000 Buried
151 S F -0.1400 Soluble
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F -0.3804 Soluble
158 N F -0.6219 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F -1.2622 Soluble
162 R F -1.8003 Soluble
163 E F -2.6847 Soluble
164 A F -2.0608 Soluble
165 K F -2.1784 Soluble
166 V F -1.0518 Soluble
167 Q F -0.5824 Soluble
168 W F 0.0000 Buried
169 K F -0.6909 Soluble
170 V F 0.0000 Buried
171 D F -1.8611 Soluble
172 N F -1.4712 Soluble
173 A F -0.4152 Soluble
174 L F 0.7425 Aggregation-prone
175 Q F -0.0631 Soluble
176 S F -0.4700 Soluble
177 G F -0.8887 Soluble
178 N F -0.7626 Soluble
179 S F -1.1074 Soluble
180 Q F -1.4357 Soluble
181 E F -1.4645 Soluble
182 S F -0.5183 Soluble
183 V F -0.1004 Soluble
184 T F -0.5088 Soluble
185 E F -1.4810 Soluble
186 Q F 0.0000 Buried
187 D F -1.8110 Soluble
188 S F -2.3448 Soluble
189 K F -2.7015 Soluble
190 D F -1.8386 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.6247 Soluble
199 L F 0.0000 Buried
200 T F -0.3127 Soluble
201 L F -0.6820 Soluble
202 S F -1.1855 Soluble
203 K F -2.3734 Soluble
204 A F -1.9600 Soluble
205 D F -3.0111 Soluble
206 Y F 0.0000 Buried
207 E F -4.0906 Soluble
208 K F -3.8233 Soluble
209 H F -3.6605 Soluble
210 K F -3.6076 Soluble
211 V F -2.0725 Soluble
212 Y F 0.0000 Buried
213 A F -0.8031 Soluble
214 C F 0.0000 Buried
215 E F -0.8998 Soluble
216 V F 0.0000 Buried
217 T F -1.2443 Soluble
218 H F 0.0000 Buried
219 Q F -1.4273 Soluble
220 G F -0.5414 Soluble
221 L F -0.3262 Soluble
222 S F -0.4860 Soluble
223 S F -0.4499 Soluble
224 P F -0.6147 Soluble
225 V F -0.0834 Soluble
226 T F -0.5030 Soluble
227 K F -0.7894 Soluble
228 S F -0.9666 Soluble
229 F F 0.0000 Buried
230 N F -2.6423 Soluble
231 R F -3.2262 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7353 2.4423 CSV PDB
4.5 -0.7802 2.4455 CSV PDB
5.0 -0.8329 2.4518 CSV PDB
5.5 -0.8819 2.46 CSV PDB
6.0 -0.9143 2.4667 CSV PDB
6.5 -0.9217 2.4703 CSV PDB
7.0 -0.9057 2.4717 CSV PDB
7.5 -0.8751 2.4722 CSV PDB
8.0 -0.8366 2.4721 CSV PDB
8.5 -0.7922 2.4716 CSV PDB
9.0 -0.7411 2.4698 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH