Runimotamab / 6mh2

A4DAB231_6mh2_D_C | Source PDB: 6mh2 | Chains: D, C | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7079
Max Score
2.2692
Min Score
-4.1055
Total Score
-282.45
Score CDR
-0.3715
Score non-CDR
-0.7883

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGYAMDYWGQGTLVTVSSASTKGPSVFPLATAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPASVVCLLNNFYPREAKVQWGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.1055
Maximum Score 2.2692
Average Score -0.7079
Total Score -282.449

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.1592 Soluble
2 V D -1.5016 Soluble
3 Q D -1.3637 Soluble
4 L D 0.0000 Buried
5 V D 1.0579 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.3831 Soluble
8 G D -0.9209 Soluble
9 G D -0.4841 Soluble
11 G D -0.2243 Soluble
12 L D 0.0633 Aggregation-prone
13 V D 0.0000 Buried
14 Q D -1.6856 Soluble
15 P D -1.8211 Soluble
16 G D -1.5591 Soluble
17 G D -1.1615 Soluble
18 S D -1.1735 Soluble
19 L D -1.2083 Soluble
20 R D -2.2315 Soluble
21 L D 0.0000 Buried
22 S D -0.4143 Soluble
23 C D 0.0000 Buried
24 A D -0.0429 Soluble
25 A D 0.0000 Buried
26 S D -0.8843 Soluble
27 G D -1.3431 Soluble
28 F D -1.2736 Soluble
29 N D -1.9431 Soluble
30 I D 0.0000 Buried
35 K D -2.4562 Soluble
36 D D -2.5287 Soluble
37 T D 0.0000 Buried
38 Y D -0.0669 Soluble
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.7927 Soluble
45 A D -1.1626 Soluble
46 P D -0.9320 Soluble
47 G D -1.4622 Soluble
48 K D -2.4120 Soluble
49 G D -1.6873 Soluble
50 L D 0.0000 Buried
51 E D -1.2089 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 R D -0.5007 Soluble
56 I D 0.0000 Buried
57 Y D -0.3501 Soluble
58 P D 0.0000 Buried
59 T D -1.2062 Soluble
62 N D -1.0937 Soluble
63 G D -0.3574 Soluble
64 Y D 0.4991 Aggregation-prone
65 T D -0.4075 Soluble
66 R D -1.9048 Soluble
67 Y D -1.8427 Soluble
68 A D -1.8256 Soluble
69 D D -2.6890 Soluble
70 S D -1.7030 Soluble
71 V D 0.0000 Buried
72 K D -2.8482 Soluble
74 G D -1.6852 Soluble
75 R D -1.3957 Soluble
76 F D 0.0000 Buried
77 T D -1.1650 Soluble
78 I D 0.0000 Buried
79 S D -0.1482 Soluble
80 A D -0.7409 Soluble
81 D D -1.3765 Soluble
82 T D -1.8057 Soluble
83 S D -1.5034 Soluble
84 K D -2.1601 Soluble
85 N D -1.6821 Soluble
86 T D 0.0000 Buried
87 A D 0.0000 Buried
88 Y D -0.5890 Soluble
89 L D 0.0000 Buried
90 Q D -1.5156 Soluble
91 M D 0.0000 Buried
92 N D -1.3857 Soluble
93 S D -1.2531 Soluble
94 L D 0.0000 Buried
95 R D -2.6253 Soluble
96 A D -1.8816 Soluble
97 E D -2.3552 Soluble
98 D D 0.0000 Buried
99 T D -0.6884 Soluble
100 A D 0.0000 Buried
101 V D 0.3594 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 S D 0.0000 Buried
106 R D -0.2326 Soluble
107 W D 0.0000 Buried
108 G D -0.5386 Soluble
109 Y D 1.4060 Aggregation-prone
114 A D 0.6221 Aggregation-prone
115 M D 0.0000 Buried
116 D D -0.2484 Soluble
117 Y D -0.5088 Soluble
118 W D -0.4853 Soluble
119 G D 0.0000 Buried
120 Q D -1.2940 Soluble
121 G D 0.0000 Buried
122 T D 0.1069 Aggregation-prone
123 L D 0.4214 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D 0.0000 Buried
127 S D -1.0297 Soluble
128 S D -0.7565 Soluble
129 A D -0.5576 Soluble
130 S D -0.6973 Soluble
131 T D -0.9442 Soluble
132 K D -1.6457 Soluble
133 G D -1.6131 Soluble
134 P D 0.0000 Buried
135 S D -0.2324 Soluble
136 V D 0.0000 Buried
137 F D 0.7052 Aggregation-prone
138 P D -0.2791 Soluble
139 L D 0.0000 Buried
140 A D -0.5833 Soluble
141 T D -0.0126 Soluble
142 A D 0.2092 Aggregation-prone
143 A D 0.1947 Aggregation-prone
144 L D 0.0000 Buried
145 G D 0.0000 Buried
146 C D 0.0000 Buried
147 L D 0.0000 Buried
148 V D 0.0000 Buried
149 K D -0.4320 Soluble
150 D D -0.7138 Soluble
151 Y D 0.0000 Buried
152 F D 0.0000 Buried
153 P D 0.0000 Buried
154 E D -0.3072 Soluble
155 P D -0.5939 Soluble
156 V D -0.5498 Soluble
157 T D -0.4997 Soluble
158 V D -0.1429 Soluble
159 S D -0.3437 Soluble
160 W D 0.0000 Buried
161 N D -0.7416 Soluble
162 S D -0.5945 Soluble
163 G D -0.4804 Soluble
164 A D -0.1905 Soluble
165 L D 0.0765 Aggregation-prone
166 T D -0.1371 Soluble
167 S D -0.2254 Soluble
168 G D -0.4113 Soluble
169 V D 0.2275 Aggregation-prone
170 H D -0.3260 Soluble
171 T D -0.1185 Soluble
172 F D 0.0000 Buried
173 P D -0.3505 Soluble
174 A D 0.3593 Aggregation-prone
175 V D 0.4528 Aggregation-prone
176 L D 1.3304 Aggregation-prone
177 Q D 0.1875 Aggregation-prone
178 S D -0.0652 Soluble
179 S D -0.1949 Soluble
180 G D 0.0710 Aggregation-prone
181 L D -0.0170 Soluble
182 Y D 0.3407 Aggregation-prone
183 S D 0.1263 Aggregation-prone
184 L D 0.0000 Buried
185 S D 0.0000 Buried
186 S D 0.0000 Buried
187 V D 0.0000 Buried
188 V D 0.3187 Aggregation-prone
189 S D -0.3384 Soluble
190 L D -0.2596 Soluble
191 G D -0.8768 Soluble
192 T D -0.6268 Soluble
193 Q D -1.0090 Soluble
194 T D -1.0381 Soluble
195 Y D -0.8353 Soluble
196 I D -1.3165 Soluble
197 C D 0.0000 Buried
198 N D -1.5190 Soluble
199 V D 0.0000 Buried
200 N D -1.7351 Soluble
201 H D 0.0000 Buried
202 K D -2.6521 Soluble
203 P D -1.6245 Soluble
204 S D -1.9551 Soluble
205 N D -2.5547 Soluble
206 T D -2.1019 Soluble
207 K D -2.6378 Soluble
208 V D -1.5811 Soluble
209 D D -2.6864 Soluble
210 K D -2.0301 Soluble
211 K D -2.4761 Soluble
212 V D 0.0000 Buried
213 E D -2.3138 Soluble
214 P D -0.9902 Soluble
1 D C -2.2676 Soluble
2 I C 0.0000 Buried
3 Q C -2.1575 Soluble
4 M C 0.0000 Buried
5 T C -1.3318 Soluble
6 Q C 0.0000 Buried
7 S C -0.7833 Soluble
8 P C -0.6666 Soluble
9 S C -0.9337 Soluble
10 S C -0.8681 Soluble
11 L C -0.6918 Soluble
12 S C -1.1593 Soluble
13 A C -1.2995 Soluble
14 S C -1.2334 Soluble
15 V C -0.0866 Soluble
16 G C -1.0262 Soluble
17 D C -2.1371 Soluble
18 R C -2.5174 Soluble
19 V C 0.0000 Buried
20 T C -0.6816 Soluble
21 I C 0.0000 Buried
22 T C -0.7351 Soluble
23 C C 0.0000 Buried
24 R C -2.7524 Soluble
25 A C 0.0000 Buried
26 S C -2.1773 Soluble
27 Q C -2.8424 Soluble
28 D C -2.8179 Soluble
29 V C 0.0000 Buried
36 N C -1.7216 Soluble
37 T C -0.7778 Soluble
38 A C 0.1818 Aggregation-prone
39 V C 0.0000 Buried
40 A C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C 0.0000 Buried
45 K C -2.3313 Soluble
46 P C -1.5943 Soluble
47 G C -1.6797 Soluble
48 K C -2.7199 Soluble
49 A C -1.7368 Soluble
50 P C 0.0000 Buried
51 K C -1.7354 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C 1.4049 Aggregation-prone
56 S C 0.6417 Aggregation-prone
57 A C 0.0000 Buried
65 S C 0.6862 Aggregation-prone
66 F C 2.2692 Aggregation-prone
67 L C 1.5292 Aggregation-prone
68 Y C 0.7481 Aggregation-prone
69 S C 0.0576 Aggregation-prone
70 G C -0.3803 Soluble
71 V C -0.2366 Soluble
72 P C -0.2806 Soluble
74 S C -0.3689 Soluble
75 R C -0.7589 Soluble
76 F C 0.0000 Buried
77 S C 0.0555 Aggregation-prone
78 G C 0.0618 Aggregation-prone
79 S C -0.6923 Soluble
80 R C -1.7631 Soluble
83 S C -1.2339 Soluble
84 G C -1.8106 Soluble
85 T C -2.1740 Soluble
86 D C -1.7444 Soluble
87 F C 0.0000 Buried
88 T C -0.7621 Soluble
89 L C 0.0000 Buried
90 T C -0.6377 Soluble
91 I C 0.0000 Buried
92 S C -1.5562 Soluble
93 S C -1.3317 Soluble
94 L C 0.0000 Buried
95 Q C -1.0807 Soluble
96 P C -1.3186 Soluble
97 E C -2.1584 Soluble
98 D C 0.0000 Buried
99 F C -1.0827 Soluble
100 A C 0.0000 Buried
101 T C -1.0945 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.6941 Aggregation-prone
108 Y C 0.8107 Aggregation-prone
109 T C 0.2344 Aggregation-prone
114 T C -0.4642 Soluble
115 P C -0.9370 Soluble
116 P C 0.0000 Buried
117 T C -0.6304 Soluble
118 F C -0.5063 Soluble
119 G C 0.0000 Buried
120 Q C -1.6587 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -1.7260 Soluble
124 V C 0.0000 Buried
125 E C -1.5041 Soluble
126 I C 0.0000 Buried
127 K C -1.8812 Soluble
128 R C -1.3459 Soluble
129 T C -0.2071 Soluble
130 V C 0.6914 Aggregation-prone
131 A C 0.2041 Aggregation-prone
132 A C -0.0763 Soluble
133 P C 0.0000 Buried
134 S C -0.2682 Soluble
135 V C 0.0000 Buried
136 F C 0.1430 Aggregation-prone
137 I C 0.0682 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5387 Soluble
140 P C -0.2471 Soluble
141 A C -0.8528 Soluble
142 S C -0.1604 Soluble
143 V C 0.0000 Buried
144 V C 0.0000 Buried
145 C C 0.0000 Buried
146 L C 0.0000 Buried
147 L C 0.0000 Buried
148 N C -0.5212 Soluble
149 N C -1.0142 Soluble
150 F C 0.0000 Buried
151 Y C 0.0000 Buried
152 P C -1.5950 Soluble
153 R C -2.5395 Soluble
154 E C -3.0116 Soluble
155 A C -2.2094 Soluble
156 K C -2.5414 Soluble
157 V C -1.5794 Soluble
158 Q C -1.5703 Soluble
159 W C -0.5659 Soluble
160 G C -1.2492 Soluble
161 N C -1.6186 Soluble
162 S C -1.6931 Soluble
163 Q C -2.0411 Soluble
164 E C -2.1887 Soluble
165 S C -0.9812 Soluble
166 V C -0.8311 Soluble
167 T C -1.1478 Soluble
168 E C -2.1686 Soluble
169 Q C 0.0000 Buried
170 D C -1.8547 Soluble
171 S C -1.9156 Soluble
172 K C -2.1790 Soluble
173 D C -1.6201 Soluble
174 S C -1.8203 Soluble
175 T C 0.0000 Buried
176 Y C 0.0000 Buried
177 S C 0.0000 Buried
178 L C 0.0000 Buried
179 S C 0.0000 Buried
180 S C 0.0000 Buried
181 T C -0.8510 Soluble
182 L C -0.6559 Soluble
183 T C -0.8317 Soluble
184 L C -1.0284 Soluble
185 S C -1.5591 Soluble
186 K C -2.7590 Soluble
187 A C -2.4868 Soluble
188 D C -3.0588 Soluble
189 Y C 0.0000 Buried
190 E C -4.1055 Soluble
191 K C -4.0839 Soluble
192 H C -3.1616 Soluble
193 K C -2.4635 Soluble
194 V C 0.2957 Aggregation-prone
195 Y C 0.0000 Buried
196 A C -0.3266 Soluble
197 C C 0.0000 Buried
198 E C -1.5389 Soluble
199 V C 0.0000 Buried
200 T C -1.4397 Soluble
201 H C 0.0000 Buried
202 Q C -1.6880 Soluble
203 G C -0.3835 Soluble
204 L C -0.1402 Soluble
205 S C -0.4402 Soluble
206 S C -0.4234 Soluble
207 P C -0.7036 Soluble
208 V C -0.2461 Soluble
209 T C -0.7779 Soluble
210 K C -0.8198 Soluble
211 S C -0.3063 Soluble
212 F C -0.5849 Soluble
213 N C -1.4589 Soluble
214 R C -2.5515 Soluble
215 G C -2.1244 Soluble
216 E C -2.0624 Soluble
217 C C -0.4826 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7307 3.839 CSV PDB
4.5 -0.7707 3.839 CSV PDB
5.0 -0.8182 3.839 CSV PDB
5.5 -0.8631 3.839 CSV PDB
6.0 -0.8937 3.839 CSV PDB
6.5 -0.9006 3.839 CSV PDB
7.0 -0.8833 3.839 CSV PDB
7.5 -0.8494 3.839 CSV PDB
8.0 -0.8059 3.8389 CSV PDB
8.5 -0.7555 3.8387 CSV PDB
9.0 -0.6991 3.838 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH