Valanafusp / 4zxb

A4DAB287_4zxb_C_D | Source PDB: 4zxb | Chains: C, D | Done
99.55
Yes
10 Å
Yes
Avg A4D Score
-0.6496
Max Score
2.4016
Min Score
-3.5862
Total Score
-239.7
Score CDR
-0.6435
Score non-CDR
-0.6507

Calculation Parameters

Chain Sequence(s) QVQLQQSGPELVKPGALVKISCKASGYTFTNYDIHWVKQRPGQGLEWIGWIYPGDGSTKYNEKFKGKATLTADKSSSTAYMHLSEKSAVYFCAREWAYWGQGTLVTVSAAKTTAPSVYPLAPSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTCNVAHPASSTKVDKKDIQMTQSPSSLSASLGERVSLTCRASQDIGGNLYWLQQGPDGTIKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISSLESEDFVDYYCLQYSSSPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKVLNSWTDQDSKDSTYSMSSTLATHKTSTSPIVKSF input.pdb
Selected Chain(s) C (Heavy), D (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5862
Maximum Score 2.4016
Average Score -0.6496
Total Score -239.699

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q C -1.4426 Soluble
2 V C -0.7856 Soluble
3 Q C -1.6139 Soluble
4 L C 0.0000 Buried
5 Q C -2.2499 Soluble
6 Q C -1.5323 Soluble
7 S C -1.1795 Soluble
8 G C -0.6697 Soluble
9 P C -0.1461 Soluble
11 E C -0.3884 Soluble
12 L C 0.6539 Aggregation-prone
13 V C -0.0068 Soluble
14 K C -1.4758 Soluble
15 P C -1.2945 Soluble
16 G C -0.8065 Soluble
17 A C 0.0228 Aggregation-prone
18 L C 0.5400 Aggregation-prone
19 V C 0.0000 Buried
20 K C -1.6081 Soluble
21 I C 0.0000 Buried
22 S C -1.0263 Soluble
23 C C 0.0000 Buried
24 K C -1.9986 Soluble
25 A C -1.2913 Soluble
26 S C -1.2180 Soluble
27 G C -0.9554 Soluble
28 Y C -0.4662 Soluble
29 T C -0.5512 Soluble
30 F C 0.0000 Buried
35 T C -1.4106 Soluble
36 N C -1.3146 Soluble
37 Y C -0.4401 Soluble
38 D C -0.5622 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 V C 0.0000 Buried
43 K C -0.9427 Soluble
44 Q C -1.5102 Soluble
45 R C -2.6856 Soluble
46 P C -1.7938 Soluble
47 G C -1.5813 Soluble
48 Q C -2.1187 Soluble
49 G C -1.4614 Soluble
50 L C 0.0000 Buried
51 E C -1.1066 Soluble
52 W C 0.0000 Buried
53 I C 0.0000 Buried
54 G C 0.0000 Buried
55 W C -0.2679 Soluble
56 I C 0.0000 Buried
57 Y C -0.8407 Soluble
58 P C 0.0000 Buried
59 G C -1.7775 Soluble
62 D C -2.3573 Soluble
63 G C -1.6164 Soluble
64 S C -1.1114 Soluble
65 T C -0.7599 Soluble
66 K C -1.2117 Soluble
67 Y C -1.4830 Soluble
68 N C -2.0964 Soluble
69 E C -3.5316 Soluble
70 K C -3.3945 Soluble
71 F C 0.0000 Buried
72 K C -3.5862 Soluble
74 G C -2.6524 Soluble
75 K C -2.8363 Soluble
76 A C 0.0000 Buried
77 T C -1.1294 Soluble
78 L C 0.0000 Buried
79 T C -0.3853 Soluble
80 A C -1.1782 Soluble
81 D C -1.7469 Soluble
82 K C -2.4348 Soluble
83 S C -1.2847 Soluble
84 S C -1.1442 Soluble
85 S C -1.3165 Soluble
86 T C 0.0000 Buried
87 A C 0.0000 Buried
88 Y C -0.5951 Soluble
89 M C 0.0000 Buried
90 H C -0.9877 Soluble
91 L C 0.0000 Buried
92 S C -0.9226 Soluble
97 E C -2.6639 Soluble
98 K C -2.7556 Soluble
99 S C -1.3569 Soluble
100 A C 0.0000 Buried
101 V C -0.3359 Soluble
102 Y C 0.0000 Buried
103 F C 0.0000 Buried
104 C C 0.0000 Buried
105 A C 0.0000 Buried
106 R C -0.3341 Soluble
107 E C -0.7009 Soluble
115 W C 0.0000 Buried
116 A C -0.2491 Soluble
117 Y C -0.1581 Soluble
118 W C -0.6874 Soluble
119 G C 0.0000 Buried
120 Q C -1.7621 Soluble
121 G C -0.9455 Soluble
122 T C 0.0000 Buried
123 L C 0.2359 Aggregation-prone
124 V C 0.0000 Buried
125 T C -0.4051 Soluble
126 V C -1.0781 Soluble
127 S C 0.0000 Buried
128 A C -1.0421 Soluble
129 A C -1.3884 Soluble
130 K C -2.0293 Soluble
131 T C -0.9412 Soluble
132 T C -0.4286 Soluble
133 A C -0.2785 Soluble
134 P C -0.2299 Soluble
135 S C -0.4068 Soluble
136 V C 0.0000 Buried
137 Y C 0.0000 Buried
138 P C -1.0275 Soluble
139 L C 0.0000 Buried
140 A C 0.3003 Aggregation-prone
141 P C 0.1420 Aggregation-prone
142 S C 1.0282 Aggregation-prone
143 V C 1.9913 Aggregation-prone
144 T C 1.2980 Aggregation-prone
145 L C 1.0875 Aggregation-prone
146 G C 0.0000 Buried
147 C C 0.0000 Buried
148 L C 0.0000 Buried
149 V C 0.0000 Buried
150 K C -0.2016 Soluble
151 G C -0.3170 Soluble
152 Y C 0.0000 Buried
153 F C 0.0000 Buried
154 P C -0.7881 Soluble
155 E C -0.9775 Soluble
156 P C -0.7500 Soluble
157 V C -0.3658 Soluble
158 T C -0.3294 Soluble
159 L C -0.2872 Soluble
160 T C -0.5245 Soluble
161 W C 0.0000 Buried
162 N C -1.5550 Soluble
163 S C -1.0307 Soluble
164 G C -0.7538 Soluble
165 S C -0.6369 Soluble
166 L C -0.1448 Soluble
167 S C -0.4263 Soluble
168 S C -0.0776 Soluble
169 G C 0.0745 Aggregation-prone
170 V C 0.3710 Aggregation-prone
171 H C -0.1888 Soluble
172 T C 0.1047 Aggregation-prone
173 F C 0.0000 Buried
174 P C -0.0780 Soluble
175 A C 0.1695 Aggregation-prone
176 V C 0.3818 Aggregation-prone
177 L C 0.1090 Aggregation-prone
178 Q C -0.3671 Soluble
179 S C -0.9113 Soluble
180 D C -1.6337 Soluble
181 L C -0.7241 Soluble
182 Y C -0.1235 Soluble
183 T C 0.0000 Buried
184 L C 0.2503 Aggregation-prone
185 S C 0.0000 Buried
186 S C 0.0000 Buried
187 S C 0.0000 Buried
188 V C 0.0000 Buried
189 T C 1.3054 Aggregation-prone
190 V C 2.1899 Aggregation-prone
191 T C -1.6697 Soluble
192 C C 0.0000 Buried
193 N C -1.7329 Soluble
194 V C 0.0000 Buried
195 A C -0.9895 Soluble
196 H C 0.0000 Buried
197 P C -0.7441 Soluble
198 A C -0.3678 Soluble
199 S C -0.4527 Soluble
200 S C -0.7112 Soluble
201 T C -0.9042 Soluble
202 K C -2.0449 Soluble
203 V C -1.5879 Soluble
204 D C -2.8457 Soluble
205 K C -2.2848 Soluble
206 K C -2.5069 Soluble
1 D D -2.3249 Soluble
2 I D 0.0000 Buried
3 Q D -2.2452 Soluble
4 M D 0.0000 Buried
5 T D -1.3592 Soluble
6 Q D -1.1093 Soluble
7 S D -0.8477 Soluble
8 P D -0.5551 Soluble
9 S D -0.7040 Soluble
10 S D -0.6412 Soluble
11 L D -0.4106 Soluble
12 S D -0.8318 Soluble
13 A D 0.0000 Buried
14 S D -0.9490 Soluble
15 L D -0.6761 Soluble
16 G D -1.2235 Soluble
17 E D -1.7478 Soluble
18 R D -2.2031 Soluble
19 V D -1.0146 Soluble
20 S D -0.5365 Soluble
21 L D 0.0000 Buried
22 T D -0.9344 Soluble
23 C D 0.0000 Buried
24 R D -2.9170 Soluble
25 A D 0.0000 Buried
26 S D -2.2374 Soluble
27 Q D -2.9190 Soluble
28 D D -2.9190 Soluble
29 I D 0.0000 Buried
36 G D -1.5178 Soluble
37 G D -0.9764 Soluble
38 N D -0.7631 Soluble
39 L D 0.0000 Buried
40 Y D 0.0000 Buried
41 W D 0.0000 Buried
42 L D 0.0000 Buried
43 Q D 0.0000 Buried
44 Q D 0.0000 Buried
45 G D -1.4285 Soluble
46 P D -1.4604 Soluble
47 D D -2.1809 Soluble
48 G D -1.5042 Soluble
49 T D -1.4462 Soluble
50 I D 0.0000 Buried
51 K D -1.5006 Soluble
52 R D -0.7371 Soluble
53 L D 0.0000 Buried
54 I D 0.0000 Buried
55 Y D 0.4541 Aggregation-prone
56 A D 0.0528 Aggregation-prone
57 T D -0.3475 Soluble
65 S D -0.3136 Soluble
66 S D 0.0784 Aggregation-prone
67 L D -0.2315 Soluble
68 D D -0.6584 Soluble
69 S D -0.5542 Soluble
70 G D -0.8731 Soluble
71 V D 0.0000 Buried
72 P D -1.1919 Soluble
74 K D -2.0004 Soluble
75 R D -1.6809 Soluble
76 F D 0.0000 Buried
77 S D -0.6710 Soluble
78 G D -0.3865 Soluble
79 S D -0.8870 Soluble
80 R D -1.7050 Soluble
83 S D -1.1225 Soluble
84 G D -1.5672 Soluble
85 S D -2.0934 Soluble
86 D D -1.8837 Soluble
87 Y D 0.0000 Buried
88 S D -0.8723 Soluble
89 L D 0.0000 Buried
90 T D -0.6063 Soluble
91 I D 0.0000 Buried
92 S D -1.6217 Soluble
93 S D -1.6501 Soluble
94 L D 0.0000 Buried
95 E D -2.1188 Soluble
96 S D -1.6296 Soluble
97 E D -2.2989 Soluble
98 D D 0.0000 Buried
99 F D 0.0000 Buried
100 V D 0.0000 Buried
101 D D -0.7928 Soluble
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 L D 0.0000 Buried
106 Q D 0.0000 Buried
107 Y D 0.0248 Aggregation-prone
108 S D -0.7634 Soluble
109 S D -0.8310 Soluble
114 S D -0.6048 Soluble
115 P D -0.8605 Soluble
116 W D -0.2862 Soluble
117 T D -0.5836 Soluble
118 F D -0.2324 Soluble
119 G D 0.0000 Buried
120 G D -0.9409 Soluble
121 G D -0.8187 Soluble
122 T D 0.0000 Buried
123 K D -0.9872 Soluble
124 L D 0.0000 Buried
125 E D -1.1304 Soluble
126 I D -0.6992 Soluble
127 K D -1.7605 Soluble
128 R D -1.9290 Soluble
129 A D -1.4876 Soluble
130 D D -2.5471 Soluble
131 A D -1.4129 Soluble
132 A D -0.6686 Soluble
133 P D 0.0000 Buried
134 T D -0.0240 Soluble
135 V D 0.2794 Aggregation-prone
136 S D 0.3394 Aggregation-prone
137 I D 0.6283 Aggregation-prone
138 F D 0.0000 Buried
139 P D 0.3891 Aggregation-prone
140 P D 0.1910 Aggregation-prone
141 S D 0.0000 Buried
142 S D -0.5397 Soluble
143 E D -1.3624 Soluble
144 Q D 0.0000 Buried
145 L D 0.4475 Aggregation-prone
146 T D -0.2756 Soluble
147 S D -0.4887 Soluble
148 G D -0.4157 Soluble
149 G D -0.2629 Soluble
150 A D 0.3204 Aggregation-prone
151 S D 0.0000 Buried
152 V D 1.0511 Aggregation-prone
153 V D 0.0000 Buried
154 C D 0.3024 Aggregation-prone
155 F D 0.0000 Buried
156 L D 0.0000 Buried
157 N D 0.0000 Buried
158 N D -0.7472 Soluble
159 F D 0.0000 Buried
160 Y D 0.0000 Buried
161 P D -2.3622 Soluble
162 K D -2.8908 Soluble
163 D D -2.8687 Soluble
164 I D -1.7739 Soluble
165 N D -2.0293 Soluble
166 V D -0.7774 Soluble
167 K D -1.3913 Soluble
168 V D 2.2202 Aggregation-prone
169 L D 1.2515 Aggregation-prone
170 N D 0.5196 Aggregation-prone
171 S D 0.1015 Aggregation-prone
172 W D 0.1103 Aggregation-prone
173 T D -0.8811 Soluble
174 D D -2.1064 Soluble
175 Q D -1.8344 Soluble
176 D D -1.8900 Soluble
177 S D -1.6980 Soluble
178 K D -2.2081 Soluble
179 D D -1.6467 Soluble
180 S D -1.7966 Soluble
181 T D 0.0000 Buried
182 Y D 0.0000 Buried
183 S D 0.0000 Buried
184 M D 0.0000 Buried
185 S D 0.0000 Buried
186 S D 0.0000 Buried
187 T D 0.0000 Buried
188 L D 2.4016 Aggregation-prone
189 A D -0.2377 Soluble
190 T D -0.9211 Soluble
191 H D 0.0000 Buried
192 K D -2.5782 Soluble
193 T D -1.2777 Soluble
194 S D -0.6533 Soluble
195 T D -0.5156 Soluble
196 S D -0.2228 Soluble
197 P D -0.2115 Soluble
198 I D 0.9077 Aggregation-prone
199 V D 1.1557 Aggregation-prone
200 K D 0.1122 Aggregation-prone
201 S D 0.6335 Aggregation-prone
202 F D 1.6085 Aggregation-prone

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6547 3.5674 CSV PDB
4.5 -0.6998 3.568 CSV PDB
5.0 -0.7493 3.57 CSV PDB
5.5 -0.7931 3.5759 CSV PDB
6.0 -0.8197 3.5917 CSV PDB
6.5 -0.821 3.6255 CSV PDB
7.0 -0.7987 3.6793 CSV PDB
7.5 -0.7616 3.7459 CSV PDB
8.0 -0.716 3.8181 CSV PDB
8.5 -0.6631 3.8922 CSV PDB
9.0 -0.6022 3.9666 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH