Nesfrotamig / 7mn8

A4DAB178_7mn8_D_C | Source PDB: 7mn8 | Chains: D, C | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.717
Max Score
2.2311
Min Score
-3.1975
Total Score
-311.2
Score CDR
-0.6291
Score non-CDR
-0.7277

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.1975
Maximum Score 2.2311
Average Score -0.717
Total Score -311.1982

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.0190 Soluble
2 V D -1.2335 Soluble
3 Q D -1.3672 Soluble
4 L D 0.0000 Buried
5 V D 0.4040 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.4576 Soluble
8 G D -0.8725 Soluble
9 G D -0.4837 Soluble
11 G D -0.4322 Soluble
12 L D 0.0010 Aggregation-prone
13 V D -0.6670 Soluble
14 Q D -1.6913 Soluble
15 P D -1.9817 Soluble
16 G D -1.5635 Soluble
17 G D -1.2814 Soluble
18 S D -1.3045 Soluble
19 L D -1.0909 Soluble
20 R D -1.8714 Soluble
21 L D 0.0000 Buried
22 S D -0.3649 Soluble
23 C D 0.0000 Buried
24 A D -0.2751 Soluble
25 A D 0.0000 Buried
26 S D -0.9750 Soluble
27 G D -1.2251 Soluble
28 F D -1.4194 Soluble
29 N D -2.3993 Soluble
30 I D 0.0000 Buried
35 K D -2.7597 Soluble
36 D D -3.0595 Soluble
37 T D 0.0000 Buried
38 Y D -0.1666 Soluble
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.8510 Soluble
45 A D -1.2245 Soluble
46 P D -0.9890 Soluble
47 G D -1.4629 Soluble
48 K D -2.4171 Soluble
49 G D -1.6462 Soluble
50 L D 0.0000 Buried
51 E D -1.2490 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 R D -0.2925 Soluble
56 I D 0.0000 Buried
57 Y D -0.5023 Soluble
58 P D -1.0778 Soluble
59 T D -1.2757 Soluble
62 N D -1.2020 Soluble
63 G D -0.4256 Soluble
64 Y D 0.5502 Aggregation-prone
65 T D -0.1820 Soluble
66 R D -1.3523 Soluble
67 Y D -1.5290 Soluble
68 A D 0.0000 Buried
69 D D -2.5765 Soluble
70 S D -1.6463 Soluble
71 V D 0.0000 Buried
72 K D -2.7905 Soluble
74 G D -1.8020 Soluble
75 R D -1.3994 Soluble
76 F D 0.0000 Buried
77 T D -0.9824 Soluble
78 I D 0.0000 Buried
79 S D -0.1806 Soluble
80 A D -0.8868 Soluble
81 D D -1.5400 Soluble
82 T D -1.9793 Soluble
83 S D -1.6601 Soluble
84 K D -2.2580 Soluble
85 N D -1.8957 Soluble
86 T D 0.0000 Buried
87 A D 0.0000 Buried
88 Y D -0.4599 Soluble
89 L D 0.0000 Buried
90 Q D -1.1088 Soluble
91 M D 0.0000 Buried
92 N D -1.4999 Soluble
93 S D -1.4271 Soluble
94 L D 0.0000 Buried
95 R D -2.9308 Soluble
96 A D -2.0341 Soluble
97 E D -2.4032 Soluble
98 D D 0.0000 Buried
99 T D -0.7872 Soluble
100 A D 0.0000 Buried
101 V D 0.1948 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 S D 0.0000 Buried
106 R D 0.0000 Buried
107 W D 0.0000 Buried
108 G D 0.0000 Buried
109 G D -1.4785 Soluble
110 D D -2.2880 Soluble
111 G D -1.1640 Soluble
112 F D -0.3632 Soluble
113 Y D 0.4031 Aggregation-prone
114 A D 0.0000 Buried
115 M D 0.0000 Buried
116 D D -0.3567 Soluble
117 Y D -0.4285 Soluble
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.6440 Soluble
121 G D -0.6808 Soluble
122 T D -0.1073 Soluble
123 L D 0.1618 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D 0.0000 Buried
127 S D -1.1541 Soluble
128 S D -0.9340 Soluble
129 A D -0.5331 Soluble
130 S D -0.6530 Soluble
131 T D -0.7605 Soluble
132 K D -1.5406 Soluble
133 G D -1.6386 Soluble
134 P D 0.0000 Buried
135 S D -0.5860 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.3127 Soluble
139 L D 0.0000 Buried
140 A D -0.8077 Soluble
141 P D 0.0000 Buried
142 S D -0.7381 Soluble
143 S D -0.6402 Soluble
144 K D -1.1087 Soluble
145 S D 0.0000 Buried
146 T D -0.7154 Soluble
147 S D -0.6728 Soluble
148 G D -0.7878 Soluble
149 G D -0.8489 Soluble
150 T D -0.5750 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D -0.4265 Soluble
159 D D -0.5421 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.6678 Soluble
164 P D -0.8703 Soluble
165 V D 0.0000 Buried
166 T D -0.9488 Soluble
167 V D -0.2821 Soluble
168 S D -0.3269 Soluble
169 W D 0.0000 Buried
170 N D -0.7211 Soluble
171 S D -0.6233 Soluble
172 G D -0.5314 Soluble
173 A D -0.2311 Soluble
174 L D -0.0290 Soluble
175 T D -0.2218 Soluble
176 S D -0.2252 Soluble
177 G D -0.2297 Soluble
178 V D -0.0290 Soluble
179 H D -0.6360 Soluble
180 T D -0.2260 Soluble
181 F D 0.0000 Buried
182 P D -0.4918 Soluble
183 A D 0.1054 Aggregation-prone
184 V D 0.4722 Aggregation-prone
185 L D 1.1422 Aggregation-prone
186 Q D 0.2007 Aggregation-prone
187 S D -0.1560 Soluble
188 S D -0.2299 Soluble
189 G D -0.0693 Soluble
190 L D -0.0477 Soluble
191 Y D 0.2431 Aggregation-prone
192 S D -0.0986 Soluble
193 L D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.1737 Soluble
199 V D 0.0000 Buried
200 P D -0.5196 Soluble
201 S D -0.5254 Soluble
202 S D -0.5105 Soluble
203 S D -0.4873 Soluble
204 L D -0.5440 Soluble
205 G D -0.9156 Soluble
206 T D -0.7532 Soluble
207 Q D -0.9842 Soluble
208 T D -0.8484 Soluble
209 Y D 0.0000 Buried
210 I D -1.2075 Soluble
211 C D 0.0000 Buried
212 N D -1.6301 Soluble
213 V D 0.0000 Buried
214 N D -2.5859 Soluble
215 H D 0.0000 Buried
216 K D -2.9075 Soluble
217 P D -1.7359 Soluble
218 S D -2.0144 Soluble
219 N D -2.6572 Soluble
220 T D -2.3248 Soluble
221 K D -2.9907 Soluble
222 V D -1.7464 Soluble
223 D D -2.6941 Soluble
224 K D -2.0281 Soluble
225 K D -2.4389 Soluble
226 V D 0.0000 Buried
227 E D -1.7377 Soluble
228 P D -0.9066 Soluble
1 D C -2.1472 Soluble
2 I C 0.0000 Buried
3 Q C -2.1820 Soluble
4 M C 0.0000 Buried
5 T C -1.3807 Soluble
6 Q C 0.0000 Buried
7 S C -0.9884 Soluble
8 P C -0.7637 Soluble
9 S C -1.0997 Soluble
10 S C -0.9593 Soluble
11 L C -0.7008 Soluble
12 S C -0.8872 Soluble
13 A C 0.0000 Buried
14 S C -0.9691 Soluble
15 V C -0.2423 Soluble
16 G C -0.8510 Soluble
17 D C -2.1343 Soluble
18 R C -2.5031 Soluble
19 V C 0.0000 Buried
20 T C -0.5334 Soluble
21 I C 0.0000 Buried
22 T C -0.7974 Soluble
23 C C 0.0000 Buried
24 R C -2.7919 Soluble
25 A C 0.0000 Buried
26 S C -2.2855 Soluble
27 Q C -2.9854 Soluble
28 D C -3.0399 Soluble
29 V C 0.0000 Buried
36 N C -2.0482 Soluble
37 T C -0.8077 Soluble
38 A C 0.0000 Buried
39 V C 0.0000 Buried
40 A C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C -1.0281 Soluble
44 Q C -1.4375 Soluble
45 K C -1.7852 Soluble
46 P C -1.4448 Soluble
47 G C -1.6038 Soluble
48 K C -2.4707 Soluble
49 A C 0.0000 Buried
50 P C 0.0000 Buried
51 K C -1.4316 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C 1.1280 Aggregation-prone
56 S C 0.4869 Aggregation-prone
57 A C 0.0000 Buried
65 S C 0.7269 Aggregation-prone
66 F C 2.2311 Aggregation-prone
67 L C 1.4939 Aggregation-prone
68 Y C 0.8714 Aggregation-prone
69 S C 0.0811 Aggregation-prone
70 G C -0.5871 Soluble
71 V C -0.1811 Soluble
72 P C -0.3207 Soluble
74 S C -0.4654 Soluble
75 R C -0.8986 Soluble
76 F C 0.0000 Buried
77 S C 0.2438 Aggregation-prone
78 G C 0.0938 Aggregation-prone
79 S C -0.5705 Soluble
80 R C -1.6149 Soluble
83 S C -1.4484 Soluble
84 G C -1.9645 Soluble
85 T C -2.4469 Soluble
86 D C -2.1355 Soluble
87 F C 0.0000 Buried
88 T C -0.7717 Soluble
89 L C 0.0000 Buried
90 T C -0.6391 Soluble
91 I C 0.0000 Buried
92 S C -1.4607 Soluble
93 S C -1.2540 Soluble
94 L C 0.0000 Buried
95 Q C -1.1270 Soluble
96 P C -1.5748 Soluble
97 E C -2.1264 Soluble
98 D C 0.0000 Buried
99 F C -1.2919 Soluble
100 A C 0.0000 Buried
101 T C -1.0971 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.0000 Buried
108 Y C 0.5015 Aggregation-prone
109 T C 0.1865 Aggregation-prone
114 T C -0.3570 Soluble
115 P C -0.7827 Soluble
116 P C 0.0000 Buried
117 T C -0.6264 Soluble
118 F C -0.5085 Soluble
119 G C 0.0000 Buried
120 Q C -1.6371 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -1.8447 Soluble
124 V C 0.0000 Buried
125 E C 0.0000 Buried
126 I C 0.0000 Buried
127 K C -1.4739 Soluble
128 R C -0.9796 Soluble
129 T C -0.2628 Soluble
130 V C 0.0554 Aggregation-prone
131 A C -0.1654 Soluble
132 A C -0.3703 Soluble
133 P C -0.4989 Soluble
134 S C -0.3890 Soluble
135 V C 0.0000 Buried
136 F C 0.0000 Buried
137 I C 0.0000 Buried
138 F C 0.0000 Buried
139 P C 0.0000 Buried
140 P C 0.0000 Buried
141 S C 0.0000 Buried
142 D C -2.8755 Soluble
143 E C -2.9232 Soluble
144 Q C 0.0000 Buried
145 L C -2.2512 Soluble
146 K C -2.8895 Soluble
147 S C -1.8966 Soluble
148 G C -1.4704 Soluble
149 T C -1.1201 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -1.4969 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.4579 Soluble
162 R C -1.8480 Soluble
163 E C -2.6656 Soluble
164 A C -2.1370 Soluble
165 K C -2.3068 Soluble
166 V C -1.3995 Soluble
167 Q C -0.7750 Soluble
168 W C 0.0000 Buried
169 K C -0.7224 Soluble
170 V C 0.0000 Buried
171 D C -1.6129 Soluble
172 N C -1.3689 Soluble
173 A C -0.3221 Soluble
174 L C 0.6481 Aggregation-prone
175 Q C -0.1096 Soluble
176 S C -0.4792 Soluble
177 G C -0.8965 Soluble
178 N C -0.9173 Soluble
179 S C -1.1211 Soluble
180 Q C -1.2854 Soluble
181 E C -1.7829 Soluble
182 S C -0.5579 Soluble
183 V C -0.3438 Soluble
184 T C -0.8441 Soluble
185 E C -1.8105 Soluble
186 Q C 0.0000 Buried
187 D C -2.1700 Soluble
188 S C -2.2247 Soluble
189 K C -2.5948 Soluble
190 D C -2.0354 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.7229 Soluble
199 L C 0.0000 Buried
200 T C -0.5179 Soluble
201 L C 0.0000 Buried
202 S C -1.0350 Soluble
203 K C -2.0680 Soluble
204 A C -1.7075 Soluble
205 D C -2.0408 Soluble
206 Y C 0.0000 Buried
207 E C -3.1975 Soluble
208 K C -3.1808 Soluble
209 H C -2.4009 Soluble
210 K C -2.2507 Soluble
211 V C -0.8901 Soluble
212 Y C 0.0000 Buried
213 A C -0.6843 Soluble
214 C C 0.0000 Buried
215 E C -1.0426 Soluble
216 V C 0.0000 Buried
217 T C -1.2203 Soluble
218 H C -1.4396 Soluble
219 Q C -1.6132 Soluble
220 G C -0.4897 Soluble
221 L C -0.2858 Soluble
222 S C -0.4588 Soluble
223 S C -0.4337 Soluble
224 P C -0.3162 Soluble
225 V C -0.1180 Soluble
226 T C -0.4477 Soluble
227 K C -0.6344 Soluble
228 S C -0.4984 Soluble
229 F C 0.0000 Buried
230 N C -1.4828 Soluble
231 R C -2.1196 Soluble
232 G C -2.1308 Soluble
233 E C -2.1301 Soluble
234 C C -1.2450 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7221 4.0094 CSV PDB
4.5 -0.765 4.0094 CSV PDB
5.0 -0.8149 4.0094 CSV PDB
5.5 -0.8622 4.0094 CSV PDB
6.0 -0.8958 4.0094 CSV PDB
6.5 -0.9072 4.0094 CSV PDB
7.0 -0.8963 4.0094 CSV PDB
7.5 -0.8705 4.0093 CSV PDB
8.0 -0.8365 4.0093 CSV PDB
8.5 -0.7963 4.0091 CSV PDB
9.0 -0.7493 4.0087 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH