Izalontamab / 5ff6

Calculation Parameters

Chain Sequence(s)	`QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGA` input.pdb
Selected Chain(s)	D (Heavy), C (Light)
Distance of Aggregation	10 Å
FoldX Energy Minimization	Yes
pH Calculations	Yes
Downloads	Download all data (.tar.gz)

Aggrescan4D Profile

Chain: Show buried residues (score = 0)

Aggregation-prone (score > 0)

Soluble (score < 0)

Buried (score = 0)

Per-Residue A4D Scores

Download CSV

Minimum Score -3.2546

Maximum Score 1.9889

Average Score -0.6898

Total Score -292.4809

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Classification:

Index	Residue	Chain	A4D Score	Classification
1	Q	D	-1.2849	Soluble
2	V	D	-0.5363	Soluble
3	Q	D	-1.0453	Soluble
4	L	D	0.0000	Buried
5	K	D	-1.5715	Soluble
6	Q	D	0.0000	Buried
7	S	D	-0.6537	Soluble
8	G	D	-0.9812	Soluble
9	P	D	-0.4675	Soluble
11	G	D	-0.3853	Soluble
12	L	D	0.0552	Aggregation-prone
13	V	D	-0.6384	Soluble
14	Q	D	-1.2945	Soluble
15	P	D	-1.4036	Soluble
16	S	D	-1.5634	Soluble
17	Q	D	-1.8575	Soluble
18	S	D	-1.6632	Soluble
19	L	D	0.0000	Buried
20	S	D	-0.7083	Soluble
21	I	D	0.0000	Buried
22	T	D	-0.4109	Soluble
23	C	D	0.0000	Buried
24	T	D	-0.8711	Soluble
25	V	D	0.0000	Buried
26	S	D	-0.7242	Soluble
27	G	D	-0.7841	Soluble
28	F	D	-0.4173	Soluble
29	S	D	-0.8447	Soluble
30	L	D	0.0000	Buried
35	T	D	-0.9083	Soluble
36	N	D	-0.8330	Soluble
37	Y	D	0.4901	Aggregation-prone
38	G	D	0.0000	Buried
39	V	D	0.0000	Buried
40	H	D	0.0000	Buried
41	W	D	0.0000	Buried
42	V	D	0.0000	Buried
43	R	D	0.0000	Buried
44	Q	D	-0.5302	Soluble
45	S	D	-1.0337	Soluble
46	P	D	-1.1893	Soluble
47	G	D	-1.7597	Soluble
48	K	D	-2.3138	Soluble
49	G	D	-1.2938	Soluble
50	L	D	0.0000	Buried
51	E	D	-0.6779	Soluble
52	W	D	0.0000	Buried
53	L	D	0.0000	Buried
54	G	D	0.0000	Buried
55	V	D	0.0000	Buried
56	I	D	0.0000	Buried
57	W	D	-0.5052	Soluble
58	S	D	-0.6335	Soluble
59	G	D	-1.2257	Soluble
63	G	D	-1.5530	Soluble
64	N	D	-1.8577	Soluble
65	T	D	-1.3057	Soluble
66	D	D	-1.0259	Soluble
67	Y	D	-0.4797	Soluble
68	N	D	-0.2183	Soluble
69	T	D	-0.2193	Soluble
70	P	D	-0.4670	Soluble
71	F	D	0.0000	Buried
72	T	D	-0.6130	Soluble
75	R	D	-1.8602	Soluble
76	L	D	0.0000	Buried
77	S	D	-1.2437	Soluble
78	I	D	0.0000	Buried
79	N	D	-1.7342	Soluble
80	K	D	-1.4642	Soluble
81	D	D	-1.8137	Soluble
82	N	D	-2.2344	Soluble
83	S	D	-1.6910	Soluble
84	K	D	-2.3676	Soluble
85	S	D	-1.5666	Soluble
86	Q	D	-1.4578	Soluble
87	V	D	0.0000	Buried
88	F	D	-0.2405	Soluble
89	F	D	0.0000	Buried
90	K	D	-1.7699	Soluble
92	N	D	-2.5167	Soluble
93	S	D	-1.8538	Soluble
94	L	D	0.0000	Buried
95	Q	D	-2.2131	Soluble
96	S	D	-1.5593	Soluble
97	N	D	-2.1162	Soluble
98	D	D	0.0000	Buried
99	T	D	-0.6341	Soluble
100	A	D	0.0000	Buried
101	I	D	0.5440	Aggregation-prone
102	Y	D	0.0000	Buried
103	Y	D	0.0000	Buried
104	C	D	0.0000	Buried
105	A	D	0.0000	Buried
106	R	D	0.0000	Buried
107	A	D	0.0000	Buried
108	L	D	1.1359	Aggregation-prone
109	T	D	1.2085	Aggregation-prone
110	Y	D	1.8925	Aggregation-prone
111	Y	D	1.9889	Aggregation-prone
112	D	D	0.0000	Buried
113	Y	D	0.6253	Aggregation-prone
114	E	D	0.0000	Buried
115	F	D	0.0000	Buried
116	A	D	0.2808	Aggregation-prone
117	Y	D	0.2556	Aggregation-prone
118	W	D	0.0000	Buried
119	G	D	0.0000	Buried
120	Q	D	-1.6382	Soluble
121	G	D	-0.8077	Soluble
122	T	D	0.0000	Buried
123	L	D	0.2334	Aggregation-prone
124	V	D	0.0000	Buried
125	T	D	-0.2760	Soluble
126	V	D	0.0000	Buried
127	S	D	-0.5937	Soluble
128	A	D	-0.2577	Soluble
129	A	D	-0.2579	Soluble
130	S	D	-0.4822	Soluble
131	T	D	-0.6603	Soluble
132	K	D	-1.3940	Soluble
133	G	D	-1.5169	Soluble
134	P	D	0.0000	Buried
135	S	D	-0.4010	Soluble
136	V	D	0.0000	Buried
137	F	D	0.0000	Buried
138	P	D	-1.2800	Soluble
139	L	D	0.0000	Buried
140	A	D	-1.1957	Soluble
141	P	D	-0.7664	Soluble
142	S	D	-0.8328	Soluble
143	G	D	-0.6419	Soluble
144	T	D	-0.4851	Soluble
145	A	D	0.0000	Buried
146	A	D	0.0000	Buried
147	L	D	0.0000	Buried
148	G	D	0.0000	Buried
149	C	D	0.0000	Buried
150	L	D	0.0000	Buried
151	V	D	0.0000	Buried
152	K	D	0.0000	Buried
153	D	D	-0.5184	Soluble
154	Y	D	0.0000	Buried
155	F	D	0.0000	Buried
156	P	D	0.0000	Buried
157	E	D	-0.4832	Soluble
158	P	D	-0.6950	Soluble
159	V	D	-0.8024	Soluble
160	T	D	-0.7294	Soluble
161	V	D	-0.4685	Soluble
162	S	D	-0.4626	Soluble
163	W	D	0.0000	Buried
164	N	D	-0.7803	Soluble
165	S	D	-0.6740	Soluble
166	G	D	-0.5141	Soluble
167	A	D	-0.2277	Soluble
168	L	D	0.0011	Aggregation-prone
169	T	D	-0.1880	Soluble
170	S	D	-0.2242	Soluble
171	G	D	-0.3128	Soluble
172	V	D	0.1048	Aggregation-prone
173	H	D	-0.3601	Soluble
174	T	D	-0.0347	Soluble
175	F	D	0.0000	Buried
176	P	D	-0.4037	Soluble
177	A	D	0.1666	Aggregation-prone
178	V	D	0.4573	Aggregation-prone
179	L	D	1.0099	Aggregation-prone
180	Q	D	0.2037	Aggregation-prone
181	S	D	-0.1017	Soluble
182	S	D	-0.2706	Soluble
183	G	D	-0.0559	Soluble
184	L	D	0.0336	Aggregation-prone
185	Y	D	0.3147	Aggregation-prone
186	S	D	0.0000	Buried
187	L	D	0.0000	Buried
188	S	D	0.0000	Buried
189	S	D	0.0000	Buried
190	V	D	0.0000	Buried
191	V	D	0.0000	Buried
192	T	D	-0.1633	Soluble
193	V	D	0.0000	Buried
194	P	D	-0.4870	Soluble
195	S	D	-0.5396	Soluble
196	S	D	-0.5981	Soluble
197	S	D	-0.5738	Soluble
198	L	D	-0.8554	Soluble
199	G	D	-0.9983	Soluble
200	T	D	-0.6972	Soluble
201	Q	D	-1.1595	Soluble
202	T	D	-1.1611	Soluble
203	Y	D	0.0000	Buried
204	I	D	-1.3568	Soluble
205	C	D	0.0000	Buried
206	N	D	-1.5660	Soluble
207	V	D	0.0000	Buried
208	N	D	-2.3422	Soluble
209	H	D	0.0000	Buried
210	K	D	-2.9292	Soluble
211	P	D	-1.6958	Soluble
212	S	D	-1.9108	Soluble
213	N	D	-2.7046	Soluble
214	T	D	-2.2021	Soluble
215	K	D	-2.8326	Soluble
216	V	D	-1.6685	Soluble
217	D	D	-2.5774	Soluble
218	K	D	-2.1981	Soluble
219	R	D	-2.6515	Soluble
220	V	D	0.0000	Buried
221	E	D	-2.8670	Soluble
222	P	D	-1.9104	Soluble
223	K	D	-2.2624	Soluble
1	D	C	-1.4433	Soluble
2	I	C	0.0000	Buried
3	L	C	0.5638	Aggregation-prone
4	L	C	0.0000	Buried
5	T	C	-0.3693	Soluble
6	Q	C	0.0000	Buried
7	S	C	0.1332	Aggregation-prone
8	P	C	0.7799	Aggregation-prone
9	V	C	1.8089	Aggregation-prone
10	I	C	1.1675	Aggregation-prone
11	L	C	0.3112	Aggregation-prone
12	S	C	-0.4784	Soluble
13	V	C	0.0000	Buried
14	S	C	-1.8311	Soluble
15	P	C	-2.0086	Soluble
16	G	C	-2.0863	Soluble
17	E	C	-2.9209	Soluble
18	R	C	-3.0191	Soluble
19	V	C	0.0000	Buried
20	S	C	-0.9245	Soluble
21	F	C	0.0000	Buried
22	S	C	-0.8423	Soluble
23	C	C	0.0000	Buried
24	R	C	-2.5403	Soluble
25	A	C	0.0000	Buried
26	S	C	-1.1399	Soluble
27	Q	C	-1.7709	Soluble
28	S	C	-1.6144	Soluble
29	I	C	0.0000	Buried
36	G	C	-1.0172	Soluble
37	T	C	-0.7672	Soluble
38	N	C	-0.7709	Soluble
39	I	C	0.0000	Buried
40	H	C	0.0000	Buried
41	W	C	0.0000	Buried
42	Y	C	0.0000	Buried
43	Q	C	0.0000	Buried
44	Q	C	-1.3074	Soluble
45	R	C	-2.0305	Soluble
46	T	C	-1.7398	Soluble
47	N	C	-1.8432	Soluble
48	G	C	-1.5838	Soluble
49	S	C	-1.0403	Soluble
50	P	C	0.0000	Buried
51	R	C	-0.7511	Soluble
52	L	C	0.0000	Buried
53	L	C	0.0000	Buried
54	I	C	0.0000	Buried
55	K	C	-0.6911	Soluble
56	Y	C	-0.5685	Soluble
57	A	C	0.0000	Buried
65	S	C	-1.1956	Soluble
66	E	C	-1.8376	Soluble
67	S	C	-1.1192	Soluble
68	I	C	-0.4821	Soluble
69	S	C	-0.3486	Soluble
70	G	C	-0.4825	Soluble
71	I	C	-0.2908	Soluble
72	P	C	-0.3879	Soluble
74	S	C	-0.5941	Soluble
75	R	C	-1.0910	Soluble
76	F	C	0.0000	Buried
77	S	C	-0.8792	Soluble
78	G	C	-0.7371	Soluble
79	S	C	-0.8709	Soluble
80	G	C	-1.1149	Soluble
83	S	C	-0.9589	Soluble
84	G	C	-1.3699	Soluble
85	T	C	-2.0159	Soluble
86	D	C	-2.6492	Soluble
87	F	C	0.0000	Buried
88	T	C	-0.9243	Soluble
89	L	C	0.0000	Buried
90	S	C	-0.8996	Soluble
91	I	C	0.0000	Buried
92	N	C	-2.1299	Soluble
93	S	C	-2.1135	Soluble
94	V	C	0.0000	Buried
95	E	C	-2.4700	Soluble
96	S	C	-2.4798	Soluble
97	E	C	-2.5216	Soluble
98	D	C	0.0000	Buried
99	I	C	0.0000	Buried
100	A	C	-1.5339	Soluble
101	D	C	-0.8281	Soluble
102	Y	C	0.0000	Buried
103	Y	C	0.0000	Buried
104	C	C	0.0000	Buried
105	Q	C	0.0000	Buried
106	Q	C	0.0000	Buried
107	N	C	-0.6055	Soluble
108	N	C	-1.1416	Soluble
109	N	C	-0.9618	Soluble
114	W	C	0.1291	Aggregation-prone
115	P	C	0.0000	Buried
116	T	C	0.0000	Buried
117	T	C	-0.0673	Soluble
118	F	C	0.1718	Aggregation-prone
119	G	C	0.0000	Buried
120	A	C	0.0293	Aggregation-prone
121	G	C	0.0502	Aggregation-prone
122	T	C	0.0000	Buried
123	K	C	-0.8577	Soluble
124	L	C	0.0000	Buried
125	E	C	-1.5235	Soluble
126	L	C	0.0000	Buried
127	K	C	-1.6865	Soluble
128	R	C	-1.2520	Soluble
129	T	C	-0.0837	Soluble
130	V	C	0.4422	Aggregation-prone
131	A	C	0.1797	Aggregation-prone
132	A	C	-0.0762	Soluble
133	P	C	0.0000	Buried
134	S	C	-0.1736	Soluble
135	V	C	0.0000	Buried
136	F	C	0.1614	Aggregation-prone
137	I	C	0.1110	Aggregation-prone
138	F	C	0.0000	Buried
139	P	C	-0.5148	Soluble
140	P	C	-0.7509	Soluble
141	S	C	-1.7134	Soluble
142	D	C	-2.7081	Soluble
143	E	C	-2.5009	Soluble
144	Q	C	0.0000	Buried
145	L	C	-2.1682	Soluble
146	K	C	-2.6430	Soluble
147	S	C	-1.6366	Soluble
148	G	C	-1.2781	Soluble
149	T	C	-0.9535	Soluble
150	A	C	0.0000	Buried
151	S	C	0.0000	Buried
152	V	C	0.0000	Buried
153	V	C	0.0000	Buried
154	C	C	0.0000	Buried
155	L	C	0.0000	Buried
156	L	C	0.0000	Buried
157	N	C	0.0000	Buried
158	N	C	-0.9798	Soluble
159	F	C	0.0000	Buried
160	Y	C	0.0000	Buried
161	P	C	-1.7510	Soluble
162	R	C	-2.9830	Soluble
163	E	C	-3.2546	Soluble
164	A	C	-2.3548	Soluble
165	K	C	-2.3056	Soluble
166	V	C	-1.2327	Soluble
167	Q	C	-0.8354	Soluble
168	W	C	0.0000	Buried
169	K	C	-0.7359	Soluble
170	V	C	0.0000	Buried
171	D	C	-1.8591	Soluble
172	N	C	-1.5672	Soluble
173	A	C	-0.4142	Soluble
174	L	C	0.3041	Aggregation-prone
175	Q	C	-0.2995	Soluble
176	S	C	-0.4789	Soluble
177	G	C	-0.8756	Soluble
178	N	C	-0.7811	Soluble
179	S	C	-1.1526	Soluble
180	Q	C	-1.4130	Soluble
181	E	C	-1.9750	Soluble
182	S	C	-1.0746	Soluble
183	V	C	-1.1574	Soluble
184	T	C	-1.2286	Soluble
185	E	C	-2.3631	Soluble
186	Q	C	0.0000	Buried
187	D	C	-2.0451	Soluble
188	S	C	-2.5015	Soluble
189	K	C	-2.7829	Soluble
190	D	C	-2.2125	Soluble
191	S	C	0.0000	Buried
192	T	C	0.0000	Buried
193	Y	C	0.0000	Buried
194	S	C	0.0000	Buried
195	L	C	0.0000	Buried
196	S	C	0.0000	Buried
197	S	C	0.0000	Buried
198	T	C	-0.7165	Soluble
199	L	C	0.0000	Buried
200	T	C	-0.4964	Soluble
201	L	C	-0.8666	Soluble
202	S	C	-1.2997	Soluble
203	K	C	-2.0930	Soluble
204	A	C	-1.8961	Soluble
205	D	C	-2.7333	Soluble
206	Y	C	0.0000	Buried
207	E	C	-3.2225	Soluble
209	H	C	-2.8557	Soluble
210	K	C	-2.9521	Soluble
211	V	C	-1.5015	Soluble
212	Y	C	0.0000	Buried
213	A	C	0.0000	Buried
214	C	C	0.0000	Buried
215	E	C	-0.9848	Soluble
216	V	C	0.0000	Buried
217	T	C	-1.2555	Soluble
218	H	C	0.0000	Buried
219	Q	C	-1.7246	Soluble
220	G	C	-0.3734	Soluble
221	L	C	-0.1946	Soluble
222	S	C	-0.4509	Soluble
223	S	C	-0.4326	Soluble
224	P	C	-0.6097	Soluble
225	V	C	-0.1627	Soluble
226	T	C	-0.5395	Soluble
227	K	C	-0.6364	Soluble
228	S	C	-0.4900	Soluble
229	F	C	-0.6363	Soluble
230	N	C	-1.5771	Soluble
231	R	C	-1.9794	Soluble
232	G	C	-1.3116	Soluble
233	A	C	-0.7045	Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer | colored by A4D score

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Downloads

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Color Legend (B-factor = A4D Score)

Score > 1.5 — highly aggregation-prone

Score 0.5–1.5 — aggregation-prone

Score ≈ 0 — buried

Score −1 to −0.5 — soluble

Score < −2.5 — highly soluble

pH Structure

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A4D Score Threshold

Highlight residues > threshold in the sequence panel

Threshold: 0.0

CDR Regions

Outline CDR regions in sequence panel

HCDR1

HCDR2

HCDR3

LCDR1

LCDR2

LCDR3

Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH	Avg A4D Score	Max A4D Score	Downloads
4.0	-0.6953	2.7013	CSV PDB
4.5	-0.7335	2.6423	CSV PDB
5.0	-0.779	2.5671	CSV PDB
5.5	-0.8222	2.4845	CSV PDB
6.0	-0.8526	2.3992	CSV PDB
6.5	-0.8628	2.3132	CSV PDB
7.0	-0.8527	2.227	CSV PDB
7.5	-0.8294	2.2038	CSV PDB
8.0	-0.7988	2.2032	CSV PDB
8.5	-0.7627	2.2013	CSV PDB
9.0	-0.7214	2.2341	CSV PDB