Suvratoxumab / 4u6v

A4DAB251_4u6v_K_M | Source PDB: 4u6v | Chains: K, M | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7106
Max Score
1.5437
Min Score
-3.8555
Total Score
-306.26
Score CDR
-0.4409
Score non-CDR
-0.7444

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSHDMHWVRQATGKGLEWVSGIGTAGDTYYPDSVKGRFTISRENAKNSLYLQMNSLRAGDTAVYYCARDRYSPTGHYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPDIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYKASSLESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCKQYADYWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) K (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8555
Maximum Score 1.5437
Average Score -0.7106
Total Score -306.2585

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E K -1.9504 Soluble
2 V K -0.9658 Soluble
3 Q K -0.9009 Soluble
4 L K 0.0000 Buried
5 V K 0.6164 Aggregation-prone
6 E K 0.0000 Buried
7 S K -0.5434 Soluble
8 G K -0.9762 Soluble
9 G K -0.6348 Soluble
11 G K -0.0905 Soluble
12 L K 0.4420 Aggregation-prone
13 V K 0.0000 Buried
14 Q K -1.6353 Soluble
15 P K -1.7319 Soluble
16 G K -1.5371 Soluble
17 G K -1.1446 Soluble
18 S K -1.3353 Soluble
19 L K -0.9030 Soluble
20 R K -1.8898 Soluble
21 L K 0.0000 Buried
22 S K -0.4044 Soluble
23 C K 0.0000 Buried
24 A K -0.2021 Soluble
25 A K 0.0000 Buried
26 S K -0.7736 Soluble
27 G K -1.1114 Soluble
28 F K -0.4315 Soluble
29 T K -0.3334 Soluble
30 F K 0.0000 Buried
35 S K -1.0057 Soluble
36 S K -0.5113 Soluble
37 H K -0.3453 Soluble
38 D K -0.5172 Soluble
39 M K 0.0000 Buried
40 H K 0.0000 Buried
41 W K 0.0000 Buried
42 V K 0.0000 Buried
43 R K -0.5651 Soluble
44 Q K -0.8525 Soluble
45 A K -0.8909 Soluble
46 T K -0.8448 Soluble
47 G K -1.4194 Soluble
48 K K -2.2810 Soluble
49 G K -1.5474 Soluble
50 L K 0.0000 Buried
51 E K -1.0944 Soluble
52 W K 0.0000 Buried
53 V K 0.0000 Buried
54 S K 0.0000 Buried
55 G K 0.0000 Buried
56 I K 0.0000 Buried
57 G K 0.0000 Buried
58 T K -0.7132 Soluble
59 A K -0.6495 Soluble
63 G K -1.1529 Soluble
64 D K -1.4995 Soluble
65 T K -0.6940 Soluble
66 Y K -0.2907 Soluble
67 Y K -0.6529 Soluble
68 P K -1.5516 Soluble
69 D K -2.8290 Soluble
70 S K -1.7943 Soluble
71 V K 0.0000 Buried
72 K K -2.5431 Soluble
74 G K -1.7702 Soluble
75 R K -1.5715 Soluble
76 F K 0.0000 Buried
77 T K -0.7343 Soluble
78 I K 0.0000 Buried
79 S K -0.6434 Soluble
80 R K -1.3148 Soluble
81 E K -1.8697 Soluble
82 N K -2.1122 Soluble
83 A K -1.6243 Soluble
84 K K -2.4879 Soluble
85 N K -2.0520 Soluble
86 S K -1.2155 Soluble
87 L K 0.0000 Buried
88 Y K -0.4519 Soluble
89 L K 0.0000 Buried
90 Q K -1.0362 Soluble
91 M K 0.0000 Buried
92 N K -1.5190 Soluble
93 S K -1.4125 Soluble
94 L K 0.0000 Buried
95 R K -2.4632 Soluble
96 A K -1.3409 Soluble
97 G K -1.0565 Soluble
98 D K 0.0000 Buried
99 T K -0.4079 Soluble
100 A K 0.0000 Buried
101 V K 0.0671 Aggregation-prone
102 Y K 0.0000 Buried
103 Y K 0.0000 Buried
104 C K 0.0000 Buried
105 A K 0.0000 Buried
106 R K 0.0000 Buried
107 D K 0.0000 Buried
108 R K -0.3091 Soluble
109 Y K 0.0739 Aggregation-prone
110 S K -0.1890 Soluble
111 P K -0.3911 Soluble
111A T K -0.3990 Soluble
112B G K -0.5510 Soluble
112A H K -0.4732 Soluble
112 Y K 0.0085 Aggregation-prone
113 Y K 0.0367 Aggregation-prone
114 G K 0.0000 Buried
115 M K 0.0000 Buried
116 D K -0.4853 Soluble
117 V K -0.2030 Soluble
118 W K 0.0000 Buried
119 G K 0.0000 Buried
120 Q K -1.4440 Soluble
121 G K -0.7057 Soluble
122 T K -0.2964 Soluble
123 T K -0.0301 Soluble
124 V K 0.0000 Buried
125 T K -0.3226 Soluble
126 V K 0.0000 Buried
127 S K -0.8689 Soluble
128 S K -0.5913 Soluble
129 A K -0.4909 Soluble
130 S K -0.6221 Soluble
131 T K -0.7500 Soluble
132 K K -1.4896 Soluble
133 G K -1.5622 Soluble
134 P K 0.0000 Buried
135 S K -0.4381 Soluble
136 V K 0.0000 Buried
137 F K 0.0000 Buried
138 P K -1.1638 Soluble
139 L K 0.0000 Buried
140 A K -0.7778 Soluble
141 P K 0.0000 Buried
142 S K -0.3310 Soluble
143 S K -0.4198 Soluble
144 G K -0.7529 Soluble
145 G K -0.8347 Soluble
146 T K -0.5610 Soluble
147 A K 0.0000 Buried
148 A K 0.0000 Buried
149 L K 0.0000 Buried
150 G K 0.0000 Buried
151 C K 0.0000 Buried
152 L K 0.0000 Buried
153 V K 0.0000 Buried
154 K K 0.0000 Buried
155 D K -0.2638 Soluble
156 Y K 0.0000 Buried
157 F K 0.0000 Buried
158 P K -0.6287 Soluble
159 E K -0.9024 Soluble
160 P K -1.0408 Soluble
161 V K -0.9157 Soluble
162 T K -0.7957 Soluble
163 V K -0.2717 Soluble
164 S K -0.4409 Soluble
165 W K 0.0000 Buried
166 N K -0.8431 Soluble
167 S K -0.6326 Soluble
168 G K -0.4540 Soluble
169 A K -0.2102 Soluble
170 L K -0.0316 Soluble
171 T K -0.1778 Soluble
172 S K -0.4300 Soluble
173 G K -0.5652 Soluble
174 V K 0.1559 Aggregation-prone
175 H K 0.0000 Buried
176 T K 0.1005 Aggregation-prone
177 F K 0.0000 Buried
178 P K -0.5038 Soluble
179 A K 0.2552 Aggregation-prone
180 V K 0.7870 Aggregation-prone
181 L K 1.5437 Aggregation-prone
182 Q K 0.5207 Aggregation-prone
183 S K 0.0428 Aggregation-prone
184 S K -0.1119 Soluble
185 G K 0.1599 Aggregation-prone
186 L K 0.1691 Aggregation-prone
187 Y K 0.5274 Aggregation-prone
188 S K 0.0000 Buried
189 L K 0.2100 Aggregation-prone
190 S K 0.0000 Buried
191 S K 0.0000 Buried
192 V K 0.0000 Buried
193 V K 0.0000 Buried
194 T K -0.1856 Soluble
195 V K 0.0000 Buried
196 P K -0.4127 Soluble
197 S K -0.5687 Soluble
198 S K -0.5735 Soluble
199 S K -0.5922 Soluble
200 L K -0.7732 Soluble
201 G K -0.9587 Soluble
202 T K -0.7815 Soluble
203 Q K -1.1703 Soluble
204 T K -1.2040 Soluble
205 Y K 0.0000 Buried
206 I K -1.2710 Soluble
207 C K 0.0000 Buried
208 N K -1.6132 Soluble
209 V K 0.0000 Buried
210 N K -2.4742 Soluble
211 H K 0.0000 Buried
212 K K -2.9023 Soluble
213 P K -1.7907 Soluble
214 S K -1.9968 Soluble
215 N K -2.6836 Soluble
216 T K -2.2732 Soluble
217 K K -2.8875 Soluble
218 V K -1.8479 Soluble
219 D K -2.5489 Soluble
220 K K -2.0418 Soluble
221 R K -2.6897 Soluble
222 V K 0.0000 Buried
223 E K -2.4338 Soluble
224 P K -1.1288 Soluble
1 D M -2.7353 Soluble
2 I M 0.0000 Buried
3 Q M -2.3408 Soluble
4 M M 0.0000 Buried
5 T M -1.4059 Soluble
6 Q M 0.0000 Buried
7 S M -0.7146 Soluble
8 P M -0.3954 Soluble
9 S M -0.6429 Soluble
10 T M -0.4922 Soluble
11 L M -0.3324 Soluble
12 S M -0.7067 Soluble
13 A M 0.0000 Buried
14 S M -0.5669 Soluble
15 V M 0.2086 Aggregation-prone
16 G M -0.9229 Soluble
17 D M -1.9800 Soluble
18 R M -2.4902 Soluble
19 V M 0.0000 Buried
20 T M -0.6350 Soluble
21 I M 0.0000 Buried
22 T M -0.9071 Soluble
23 C M 0.0000 Buried
24 R M -2.8434 Soluble
25 A M 0.0000 Buried
26 S M -2.0951 Soluble
27 Q M -2.2595 Soluble
28 S M -1.3517 Soluble
29 I M 0.0000 Buried
36 S M -0.6873 Soluble
37 S M -0.5556 Soluble
38 W M -0.1260 Soluble
39 L M 0.0000 Buried
40 A M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M 0.0000 Buried
44 Q M 0.0000 Buried
45 K M -1.7040 Soluble
46 P M -1.5244 Soluble
47 G M -1.7308 Soluble
48 K M -2.5754 Soluble
49 A M -1.6014 Soluble
50 P M 0.0000 Buried
51 K M -1.4760 Soluble
52 L M 0.0000 Buried
53 L M 0.0000 Buried
54 I M 0.0000 Buried
55 Y M -0.1225 Soluble
56 K M -0.7332 Soluble
57 A M 0.0000 Buried
65 S M -0.4967 Soluble
66 S M -0.2123 Soluble
67 L M 0.1478 Aggregation-prone
68 E M -0.2669 Soluble
69 S M -0.3422 Soluble
70 G M -0.4982 Soluble
71 V M -0.3364 Soluble
72 P M -0.3280 Soluble
74 S M -0.4106 Soluble
75 R M -0.8010 Soluble
76 F M 0.0000 Buried
77 S M -0.2780 Soluble
78 G M -0.3682 Soluble
79 S M -0.7941 Soluble
80 G M -1.2454 Soluble
83 S M -1.0088 Soluble
84 G M -1.2819 Soluble
85 T M -2.0232 Soluble
86 E M -2.7385 Soluble
87 F M 0.0000 Buried
88 T M -0.8237 Soluble
89 L M 0.0000 Buried
90 T M -0.5891 Soluble
91 I M 0.0000 Buried
92 S M -1.3656 Soluble
93 S M -1.2544 Soluble
94 L M 0.0000 Buried
95 Q M -1.1576 Soluble
96 P M -0.8771 Soluble
97 D M -1.8681 Soluble
98 D M 0.0000 Buried
99 F M -0.3853 Soluble
100 A M 0.0000 Buried
101 T M 0.0000 Buried
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 K M 0.0000 Buried
106 Q M 0.0000 Buried
107 Y M -0.0904 Soluble
108 A M -0.4654 Soluble
114 D M -1.4776 Soluble
115 Y M -0.1408 Soluble
116 W M -0.1597 Soluble
117 T M -0.7731 Soluble
118 F M 0.0000 Buried
119 G M 0.0000 Buried
120 Q M -1.4538 Soluble
121 G M 0.0000 Buried
122 T M 0.0000 Buried
123 K M -0.8393 Soluble
124 V M 0.0000 Buried
125 E M -0.7288 Soluble
126 I M -0.2201 Soluble
127 K M -1.1390 Soluble
128 R M -0.9875 Soluble
129 T M -0.1124 Soluble
130 V M 0.3406 Aggregation-prone
131 A M -0.0184 Soluble
132 A M -0.1201 Soluble
133 P M 0.0000 Buried
134 S M -0.1075 Soluble
135 V M 0.0000 Buried
136 F M 0.0706 Aggregation-prone
137 I M 0.1448 Aggregation-prone
138 F M 0.0000 Buried
139 P M -0.4997 Soluble
140 P M 0.0000 Buried
141 S M -1.5788 Soluble
142 D M -2.9198 Soluble
143 E M -3.0197 Soluble
144 Q M 0.0000 Buried
145 L M -2.3923 Soluble
146 K M -2.8840 Soluble
147 S M -1.8984 Soluble
148 G M -1.4441 Soluble
149 T M -0.9948 Soluble
150 A M 0.0000 Buried
151 S M 0.0000 Buried
152 V M 0.0000 Buried
153 V M 0.0000 Buried
154 C M 0.0000 Buried
155 L M 0.0000 Buried
156 L M 0.0000 Buried
157 N M -0.3567 Soluble
158 N M -0.8237 Soluble
159 F M 0.0000 Buried
160 Y M 0.0000 Buried
161 P M -1.7569 Soluble
162 R M -3.1580 Soluble
163 E M -3.2732 Soluble
164 A M -2.3225 Soluble
165 K M -2.2435 Soluble
166 V M -0.9946 Soluble
167 Q M -0.5122 Soluble
168 W M 0.0000 Buried
169 K M -0.6071 Soluble
170 V M 0.0000 Buried
171 D M -1.8989 Soluble
172 N M -1.3352 Soluble
173 A M -0.3361 Soluble
174 L M 0.5866 Aggregation-prone
175 Q M -0.4255 Soluble
176 S M -0.6384 Soluble
177 G M -1.1914 Soluble
178 N M -1.6970 Soluble
179 S M -1.4544 Soluble
180 Q M -1.3258 Soluble
181 E M -1.8312 Soluble
182 S M -0.8209 Soluble
183 V M -0.9656 Soluble
184 T M -1.2450 Soluble
185 E M -2.3144 Soluble
186 Q M -1.8334 Soluble
187 D M -1.8207 Soluble
188 S M -1.5899 Soluble
189 K M -2.2072 Soluble
190 D M -1.4538 Soluble
191 S M -1.5358 Soluble
192 T M 0.0000 Buried
193 Y M 0.0000 Buried
194 S M 0.0000 Buried
195 L M 0.0000 Buried
196 S M 0.0000 Buried
197 S M 0.0000 Buried
198 T M -0.7219 Soluble
199 L M 0.0000 Buried
200 T M -0.6548 Soluble
201 L M -0.9995 Soluble
202 S M -1.2919 Soluble
203 K M -2.4625 Soluble
204 A M -2.1386 Soluble
205 D M -3.0081 Soluble
206 Y M 0.0000 Buried
207 E M -3.8117 Soluble
208 K M -3.8555 Soluble
209 H M -3.0983 Soluble
210 K M -2.9162 Soluble
211 V M -1.7943 Soluble
212 Y M 0.0000 Buried
213 A M -0.5833 Soluble
214 C M 0.0000 Buried
215 E M -0.6133 Soluble
216 V M 0.0000 Buried
217 T M -1.1942 Soluble
218 H M 0.0000 Buried
219 Q M -1.6001 Soluble
220 G M -0.4312 Soluble
221 L M -0.2701 Soluble
222 S M -0.4882 Soluble
223 S M -0.4460 Soluble
224 P M -0.5167 Soluble
225 V M 0.0327 Aggregation-prone
226 T M -0.3723 Soluble
227 K M -0.5447 Soluble
228 S M -0.4079 Soluble
229 F M -0.8481 Soluble
230 N M -1.8267 Soluble
231 R M -2.3303 Soluble
232 G M -1.7141 Soluble
233 E M -1.9348 Soluble
234 C M -0.4890 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7014 2.1244 CSV PDB
4.5 -0.7445 1.9475 CSV PDB
5.0 -0.7952 1.7467 CSV PDB
5.5 -0.8439 1.6708 CSV PDB
6.0 -0.8801 1.687 CSV PDB
6.5 -0.8962 1.7021 CSV PDB
7.0 -0.8914 1.7112 CSV PDB
7.5 -0.872 1.715 CSV PDB
8.0 -0.8442 1.7164 CSV PDB
8.5 -0.81 1.7167 CSV PDB
9.0 -0.7691 1.7163 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH