Runimotamab / 6mxs

A4DAB231_6mxs_H_L | Source PDB: 6mxs | Chains: H, L | Done
93.51
Yes
10 Å
Yes
Avg A4D Score
-0.6769
Max Score
1.7666
Min Score
-3.2159
Total Score
-293.78
Score CDR
-0.2621
Score non-CDR
-0.7322

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTWIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGFMFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIQMTQSPSSLSASVGDRVTITCRASQDIPRSISGYVAWYQQKPGKAPKLLIYWGSYLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYAEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2159
Maximum Score 1.7666
Average Score -0.6769
Total Score -293.7842

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0221 Soluble
2 V H -1.0742 Soluble
3 Q H -1.1894 Soluble
4 L H 0.0000 Buried
5 V H 0.0945 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.6640 Soluble
8 G H -0.9149 Soluble
9 G H -0.4755 Soluble
11 G H -0.2545 Soluble
12 L H -0.0041 Soluble
13 V H -0.6005 Soluble
14 Q H -1.5850 Soluble
15 P H -1.7361 Soluble
16 G H -1.4805 Soluble
17 G H -1.0982 Soluble
18 S H -1.3232 Soluble
19 L H -1.2114 Soluble
20 R H -2.2510 Soluble
21 L H 0.0000 Buried
22 S H -0.6371 Soluble
23 C H 0.0000 Buried
24 A H -0.3558 Soluble
25 A H -0.6083 Soluble
26 S H -0.9353 Soluble
27 G H -1.2555 Soluble
28 F H -1.2898 Soluble
29 N H -2.1502 Soluble
30 I H 0.0000 Buried
35 K H -2.8141 Soluble
36 D H -2.4947 Soluble
37 T H 0.0000 Buried
38 W H 0.0000 Buried
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7965 Soluble
45 A H -1.1874 Soluble
46 P H -0.9471 Soluble
47 G H -1.4691 Soluble
48 K H -2.3681 Soluble
49 G H -1.6247 Soluble
50 L H 0.0000 Buried
51 E H -1.0803 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H -0.3698 Soluble
56 I H 0.0000 Buried
57 Y H -0.3331 Soluble
58 P H -1.1013 Soluble
59 T H -1.3151 Soluble
62 N H -1.1036 Soluble
63 G H -0.4219 Soluble
64 Y H 0.5848 Aggregation-prone
65 T H -0.1570 Soluble
66 R H -1.2778 Soluble
67 Y H -1.4778 Soluble
68 A H 0.0000 Buried
69 D H -2.5799 Soluble
70 S H -1.5623 Soluble
71 V H 0.0000 Buried
72 K H -2.7269 Soluble
74 G H -1.7416 Soluble
75 R H -1.5137 Soluble
76 F H 0.0000 Buried
77 T H -1.1219 Soluble
78 I H 0.0000 Buried
79 S H -0.2455 Soluble
80 A H -0.9781 Soluble
81 D H -1.5322 Soluble
82 T H -1.8573 Soluble
83 S H -1.5887 Soluble
84 K H -2.2056 Soluble
85 N H -1.7610 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.5632 Soluble
91 M H 0.0000 Buried
92 N H -1.4882 Soluble
93 S H -1.2864 Soluble
94 L H 0.0000 Buried
95 R H -2.4809 Soluble
96 A H -1.8026 Soluble
97 E H -2.2811 Soluble
98 D H 0.0000 Buried
99 T H -0.7512 Soluble
100 A H 0.0000 Buried
101 V H 0.2083 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H -0.0633 Soluble
107 W H 0.0000 Buried
108 G H 0.0000 Buried
109 G H 0.3354 Aggregation-prone
110 F H 1.7666 Aggregation-prone
111 M H 1.3659 Aggregation-prone
112 F H 1.4535 Aggregation-prone
113 Y H 1.1013 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.0564 Soluble
117 Y H 0.0488 Aggregation-prone
118 W H -0.3688 Soluble
119 G H 0.0000 Buried
120 Q H -1.4994 Soluble
121 G H -0.7332 Soluble
122 T H -0.1410 Soluble
123 L H 0.0898 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4319 Soluble
126 V H 0.0000 Buried
127 S H -0.9772 Soluble
128 S H -0.6749 Soluble
129 A H -0.4411 Soluble
130 S H -0.5401 Soluble
131 T H -0.7075 Soluble
132 K H -1.1452 Soluble
133 G H -1.3956 Soluble
134 P H 0.0000 Buried
135 S H -0.3573 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1665 Soluble
139 L H 0.0000 Buried
140 A H -1.1921 Soluble
141 P H 0.0000 Buried
142 S H -0.5778 Soluble
143 G H -0.8409 Soluble
144 G H -0.8535 Soluble
145 T H -0.5505 Soluble
146 A H 0.0000 Buried
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.3630 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.5250 Soluble
159 P H -0.8062 Soluble
160 V H -0.7591 Soluble
161 T H -0.6437 Soluble
162 V H -0.4284 Soluble
163 S H -0.4334 Soluble
164 W H 0.0000 Buried
165 N H -0.6727 Soluble
166 S H -0.6601 Soluble
167 G H -0.5790 Soluble
168 A H -0.2473 Soluble
169 L H -0.0100 Soluble
170 T H -0.1922 Soluble
171 S H -0.1951 Soluble
172 G H -0.2504 Soluble
173 V H 0.1339 Aggregation-prone
174 H H -0.3272 Soluble
175 T H 0.0309 Aggregation-prone
176 F H 0.0000 Buried
177 P H -0.2614 Soluble
178 A H 0.2807 Aggregation-prone
179 V H 0.6559 Aggregation-prone
180 L H 1.1136 Aggregation-prone
181 Q H 0.2694 Aggregation-prone
182 S H -0.0887 Soluble
183 S H -0.2467 Soluble
184 G H -0.0413 Soluble
185 L H 0.1229 Aggregation-prone
186 Y H 0.4625 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1360 Soluble
194 V H 0.0000 Buried
195 P H -0.6614 Soluble
196 S H -0.6414 Soluble
197 S H -0.7331 Soluble
198 S H -0.6496 Soluble
199 L H -0.8321 Soluble
200 G H -0.9554 Soluble
201 T H -0.7222 Soluble
202 Q H -1.2157 Soluble
203 T H -1.0305 Soluble
204 Y H 0.0000 Buried
205 I H -1.0274 Soluble
206 C H 0.0000 Buried
207 N H -1.4270 Soluble
208 V H 0.0000 Buried
209 N H -2.1400 Soluble
210 H H 0.0000 Buried
211 K H -2.8279 Soluble
212 P H -1.6413 Soluble
213 S H -1.8379 Soluble
214 N H -2.6298 Soluble
215 T H -2.1297 Soluble
216 K H -2.7546 Soluble
217 V H -1.5650 Soluble
218 D H -2.3919 Soluble
219 K H -1.9082 Soluble
220 K H -2.1369 Soluble
221 V H 0.0000 Buried
222 E H -2.7575 Soluble
223 P H -1.8770 Soluble
224 K H -2.4394 Soluble
225 S H -1.1502 Soluble
1 D L -2.1098 Soluble
2 I L -1.6017 Soluble
3 Q L -1.9304 Soluble
4 M L 0.0000 Buried
5 T L -1.2466 Soluble
6 Q L 0.0000 Buried
7 S L -0.8699 Soluble
8 P L -0.5882 Soluble
9 S L -0.9906 Soluble
10 S L -1.0141 Soluble
11 L L -0.6285 Soluble
12 S L -0.7842 Soluble
13 A L -0.9458 Soluble
14 S L -0.8987 Soluble
15 V L -0.2116 Soluble
16 G L -0.9844 Soluble
17 D L -2.0624 Soluble
18 R L -2.4635 Soluble
19 V L 0.0000 Buried
20 T L -0.6469 Soluble
21 I L 0.0000 Buried
22 T L -0.8065 Soluble
23 C L 0.0000 Buried
24 R L -2.5552 Soluble
25 A L 0.0000 Buried
26 S L -2.0867 Soluble
27 Q L -2.5540 Soluble
28 D L -2.7509 Soluble
29 I L 0.0000 Buried
30 P L -1.3301 Soluble
31 R L -1.9996 Soluble
34 S L -0.5828 Soluble
35 I L 0.6079 Aggregation-prone
36 S L 0.2587 Aggregation-prone
37 G L 0.0000 Buried
38 Y L 1.1764 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.2830 Soluble
45 K L -1.6350 Soluble
46 P L -1.4473 Soluble
47 G L -1.7794 Soluble
48 K L -2.5451 Soluble
49 A L -1.5396 Soluble
50 P L 0.0000 Buried
51 K L -1.2842 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.2390 Aggregation-prone
56 W L 1.2828 Aggregation-prone
57 G L 0.4894 Aggregation-prone
65 S L 0.6694 Aggregation-prone
66 Y L 1.7212 Aggregation-prone
67 L L 1.2858 Aggregation-prone
68 Y L 0.5454 Aggregation-prone
69 S L -0.0541 Soluble
70 G L -0.4671 Soluble
71 V L 0.0000 Buried
72 P L -0.3568 Soluble
74 S L -0.4058 Soluble
75 R L -0.7479 Soluble
76 F L 0.0000 Buried
77 S L 0.0148 Aggregation-prone
78 G L 0.0274 Aggregation-prone
79 S L -0.5261 Soluble
80 G L -1.2837 Soluble
83 S L -1.0221 Soluble
84 G L -1.8291 Soluble
85 T L -2.1171 Soluble
86 D L -1.9640 Soluble
87 F L 0.0000 Buried
88 T L -0.7848 Soluble
89 L L 0.0000 Buried
90 T L -0.6191 Soluble
91 I L 0.0000 Buried
92 S L -1.4065 Soluble
93 S L -1.2966 Soluble
94 L L 0.0000 Buried
95 Q L -1.1143 Soluble
96 P L -1.4197 Soluble
97 E L -1.5854 Soluble
98 D L 0.0000 Buried
99 F L -1.3330 Soluble
100 A L 0.0000 Buried
101 T L -1.0677 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.0000 Buried
108 Y L 0.4854 Aggregation-prone
109 T L -0.1369 Soluble
114 T L -0.2029 Soluble
115 P L -0.4869 Soluble
116 P L 0.0000 Buried
117 T L -0.3924 Soluble
118 F L -0.4692 Soluble
119 G L 0.0000 Buried
120 Q L -1.7566 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.9644 Soluble
124 V L 0.0000 Buried
125 E L -1.2806 Soluble
126 I L 0.0000 Buried
127 K L -1.2137 Soluble
128 R L -0.8533 Soluble
129 T L -0.1720 Soluble
130 V L 0.2215 Aggregation-prone
131 A L -0.0791 Soluble
132 A L -0.1589 Soluble
133 P L 0.0000 Buried
134 S L -0.1703 Soluble
135 V L 0.0000 Buried
136 F L 0.1761 Aggregation-prone
137 I L 0.1563 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4452 Soluble
140 P L -0.7688 Soluble
141 S L -1.6909 Soluble
142 D L -2.8602 Soluble
143 E L -2.4499 Soluble
144 Q L 0.0000 Buried
145 L L -2.1036 Soluble
146 K L -2.6451 Soluble
147 S L -1.6572 Soluble
148 G L -1.2131 Soluble
149 T L -0.9883 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9615 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5282 Soluble
162 R L -2.4172 Soluble
163 E L -2.8881 Soluble
164 A L -2.0299 Soluble
165 K L -2.1919 Soluble
166 V L -1.0363 Soluble
167 Q L -0.9310 Soluble
168 W L 0.0000 Buried
169 K L -1.0894 Soluble
170 V L 0.0000 Buried
171 D L -1.6680 Soluble
172 N L -1.5777 Soluble
173 A L -0.4798 Soluble
174 L L 0.3006 Aggregation-prone
175 Q L -0.3412 Soluble
176 S L -0.4797 Soluble
177 G L -0.8928 Soluble
178 N L -0.8157 Soluble
179 S L -1.0034 Soluble
180 Q L -1.1382 Soluble
181 E L -1.3973 Soluble
182 S L -0.6860 Soluble
183 V L -0.4045 Soluble
184 T L -1.0083 Soluble
185 E L -2.1517 Soluble
186 Q L 0.0000 Buried
187 D L -2.0952 Soluble
188 S L -2.1498 Soluble
189 K L -2.5626 Soluble
190 D L -1.9834 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5554 Soluble
199 L L 0.0000 Buried
200 T L -0.3229 Soluble
201 L L -0.5923 Soluble
202 S L -0.9900 Soluble
203 K L -1.9926 Soluble
204 A L -1.7293 Soluble
205 D L -2.2844 Soluble
206 Y L 0.0000 Buried
207 E L -2.9648 Soluble
208 K L -3.2159 Soluble
209 H L -2.4693 Soluble
210 K L -2.1083 Soluble
211 V L -0.6620 Soluble
212 Y L 0.0000 Buried
213 A L -0.9082 Soluble
215 E L -1.5445 Soluble
216 V L 0.0000 Buried
217 T L -1.2522 Soluble
218 H L 0.0000 Buried
219 Q L -1.6692 Soluble
220 G L -0.4333 Soluble
221 L L -0.1756 Soluble
222 S L -0.4407 Soluble
223 S L -0.4220 Soluble
224 P L -0.6959 Soluble
225 V L -0.2116 Soluble
226 T L -0.7254 Soluble
227 K L -0.8742 Soluble
228 S L -0.5558 Soluble
229 F L -0.5217 Soluble
230 N L -1.2362 Soluble
231 R L -1.8217 Soluble
232 G L -1.6797 Soluble
233 E L -1.9828 Soluble
234 C L -0.7087 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.692 4.6703 CSV PDB
4.5 -0.7363 4.6199 CSV PDB
5.0 -0.7865 4.5665 CSV PDB
5.5 -0.8331 4.5121 CSV PDB
6.0 -0.8654 4.4575 CSV PDB
6.5 -0.8744 4.4029 CSV PDB
7.0 -0.8601 4.3489 CSV PDB
7.5 -0.8305 4.2968 CSV PDB
8.0 -0.792 4.2498 CSV PDB
8.5 -0.7463 4.2137 CSV PDB
9.0 -0.6936 4.1925 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH