Solnerstotug / 8tbq

A4DAB243_8tbq_A_B | Source PDB: 8tbq | Chains: A, B | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.634
Max Score
2.6245
Min Score
-3.0243
Total Score
-249.78
Score CDR
-0.4266
Score non-CDR
-0.6641

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFDDYSMNWVRQAPGKGLEWVSYISSDADTIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAREAGGVLGRVDFQHWGQGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLQTYICNVNHKPSNTKVDKKVEPKDIQLTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLGTGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQADFVPPWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKQESVTEQDSKDSTYSLSSTLTCEVTHQGLSSPVTK input.pdb
Selected Chain(s) A (Heavy), B (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.0243
Maximum Score 2.6245
Average Score -0.634
Total Score -249.7822

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E A -2.1686 Soluble
2 V A -1.4569 Soluble
3 Q A -1.4015 Soluble
4 L A 0.0000 Buried
5 V A 1.1410 Aggregation-prone
6 E A 0.0000 Buried
7 S A -0.2922 Soluble
8 G A -0.7883 Soluble
9 G A -0.3398 Soluble
11 G A -0.2506 Soluble
12 L A -0.0515 Soluble
13 V A -0.5163 Soluble
14 Q A -1.3225 Soluble
15 P A -1.4461 Soluble
16 G A -1.4025 Soluble
17 G A -1.0491 Soluble
18 S A -0.9809 Soluble
19 L A -0.8539 Soluble
20 R A -1.7831 Soluble
21 L A 0.0000 Buried
22 S A -0.2825 Soluble
23 C A 0.0000 Buried
24 A A -0.0114 Soluble
25 A A 0.0000 Buried
26 S A -1.0506 Soluble
27 G A -1.3703 Soluble
28 F A -0.9936 Soluble
29 T A -1.0690 Soluble
30 F A 0.0000 Buried
35 D A -2.8890 Soluble
36 D A -2.7207 Soluble
37 Y A -1.2762 Soluble
38 S A -0.5861 Soluble
39 M A 0.0000 Buried
40 N A 0.0000 Buried
41 W A 0.0000 Buried
42 V A 0.0000 Buried
43 R A 0.0000 Buried
44 Q A -0.7477 Soluble
45 A A -1.1663 Soluble
46 P A -0.9631 Soluble
47 G A -1.4883 Soluble
48 K A -2.2678 Soluble
49 G A -1.4607 Soluble
50 L A 0.0000 Buried
51 E A -0.9558 Soluble
52 W A 0.0000 Buried
53 V A 0.0000 Buried
54 S A 0.0000 Buried
55 Y A 0.5287 Aggregation-prone
56 I A 0.0000 Buried
57 S A -1.2183 Soluble
58 S A -2.1538 Soluble
59 D A -3.0243 Soluble
62 A A -1.8661 Soluble
63 D A -2.1841 Soluble
64 T A -0.7610 Soluble
65 I A 0.5053 Aggregation-prone
66 Y A 0.6025 Aggregation-prone
67 Y A -0.5168 Soluble
68 A A -1.3338 Soluble
69 D A -2.4618 Soluble
70 S A -1.7330 Soluble
71 V A 0.0000 Buried
72 K A -2.5738 Soluble
74 G A -1.7102 Soluble
75 R A -1.1917 Soluble
76 F A 0.0000 Buried
77 T A -0.6138 Soluble
78 I A 0.0000 Buried
79 S A -0.1772 Soluble
80 R A -1.1853 Soluble
81 D A -1.6693 Soluble
82 N A -2.1793 Soluble
83 A A -1.4096 Soluble
84 K A -2.3335 Soluble
85 N A -1.8976 Soluble
86 S A -1.0442 Soluble
87 L A 0.0000 Buried
88 Y A -0.3963 Soluble
89 L A 0.0000 Buried
90 Q A -0.9964 Soluble
91 M A 0.0000 Buried
92 N A -1.2787 Soluble
93 S A -1.2335 Soluble
94 L A 0.0000 Buried
95 R A -2.3270 Soluble
96 A A -1.7427 Soluble
97 E A -2.2366 Soluble
98 D A 0.0000 Buried
99 T A -0.7524 Soluble
100 A A 0.0000 Buried
101 V A 0.2448 Aggregation-prone
102 Y A 0.0000 Buried
103 Y A 0.0000 Buried
104 C A 0.0000 Buried
105 A A 0.0000 Buried
106 R A 0.0000 Buried
107 E A 0.0000 Buried
108 A A 0.0000 Buried
109 G A 0.0181 Aggregation-prone
110 G A 0.5238 Aggregation-prone
111 V A 2.0656 Aggregation-prone
111A L A 2.0086 Aggregation-prone
112A G A 0.7141 Aggregation-prone
112 R A 0.3928 Aggregation-prone
113 V A 0.6149 Aggregation-prone
114 D A 0.0000 Buried
115 F A 0.0000 Buried
116 Q A -0.5751 Soluble
117 H A -0.6417 Soluble
118 W A -0.6528 Soluble
119 G A 0.0000 Buried
120 Q A -1.2709 Soluble
121 G A 0.0000 Buried
122 T A 0.0688 Aggregation-prone
123 L A 0.0725 Aggregation-prone
124 V A 0.0000 Buried
125 T A -0.4661 Soluble
126 V A 0.0000 Buried
127 S A -0.6680 Soluble
128 S A -0.6254 Soluble
129 A A -0.4106 Soluble
130 S A -0.5759 Soluble
131 T A -0.7828 Soluble
132 K A -1.5466 Soluble
133 G A -1.6008 Soluble
134 P A -0.7514 Soluble
135 S A -0.5045 Soluble
136 V A 0.0000 Buried
137 F A 0.0000 Buried
138 P A -1.2496 Soluble
139 L A 0.0000 Buried
140 A A -1.2749 Soluble
141 P A -0.6822 Soluble
142 T A -0.2125 Soluble
143 A A -0.0983 Soluble
144 A A 0.0000 Buried
145 L A 0.0000 Buried
146 G A 0.0000 Buried
147 C A 0.0000 Buried
148 L A 0.0000 Buried
149 V A 0.0000 Buried
150 K A 0.0000 Buried
151 D A -0.7430 Soluble
152 Y A 0.0000 Buried
153 F A 0.0000 Buried
154 P A 0.0000 Buried
155 E A -0.8365 Soluble
156 P A -0.8119 Soluble
157 V A -0.7248 Soluble
158 T A -0.6113 Soluble
159 V A -0.3988 Soluble
160 S A -0.3975 Soluble
161 W A 0.0000 Buried
162 N A -0.8636 Soluble
163 S A -0.6357 Soluble
164 G A -0.4657 Soluble
165 A A -0.2189 Soluble
166 L A -0.0023 Soluble
167 T A -0.1840 Soluble
168 S A -0.2013 Soluble
169 G A -0.2450 Soluble
170 V A 0.1118 Aggregation-prone
171 H A -0.3481 Soluble
172 T A 0.0207 Aggregation-prone
173 F A 0.0000 Buried
174 P A -0.1330 Soluble
175 A A 0.3380 Aggregation-prone
176 V A 0.3060 Aggregation-prone
177 L A 0.9893 Aggregation-prone
178 Q A -0.0323 Soluble
179 S A -0.3341 Soluble
180 S A -0.3270 Soluble
181 G A -0.0914 Soluble
182 L A -0.0713 Soluble
183 Y A 0.2443 Aggregation-prone
184 S A 0.0000 Buried
185 L A 0.0000 Buried
186 S A 0.0000 Buried
187 S A 0.0000 Buried
188 V A 0.0000 Buried
189 V A 0.0000 Buried
190 T A -0.1910 Soluble
191 V A 0.0000 Buried
192 P A -0.2953 Soluble
193 S A -0.2325 Soluble
194 S A -0.2991 Soluble
195 S A -0.2364 Soluble
196 L A 0.0228 Aggregation-prone
197 Q A -1.0617 Soluble
198 T A -1.0785 Soluble
199 Y A 0.0000 Buried
200 I A -1.3151 Soluble
201 C A 0.0000 Buried
202 N A -1.4635 Soluble
203 V A 0.0000 Buried
204 N A -2.0485 Soluble
205 H A 0.0000 Buried
206 K A -2.8198 Soluble
207 P A -1.6910 Soluble
208 S A -1.9137 Soluble
209 N A -2.6642 Soluble
210 T A -2.0823 Soluble
211 K A -2.6895 Soluble
212 V A -1.4795 Soluble
213 D A -2.4569 Soluble
214 K A -1.9637 Soluble
215 K A -2.5237 Soluble
216 V A 0.0000 Buried
217 E A -2.5109 Soluble
218 P A -1.7624 Soluble
219 K A -2.4712 Soluble
1 D B -2.0840 Soluble
2 I B 0.0000 Buried
3 Q B -2.0759 Soluble
4 L B 0.0000 Buried
5 T B -0.9176 Soluble
6 Q B 0.0000 Buried
7 S B -0.5448 Soluble
8 P B -0.5717 Soluble
9 S B -0.7832 Soluble
10 S B -0.8471 Soluble
11 L B -0.6522 Soluble
12 S B -0.8630 Soluble
13 A B -1.0748 Soluble
14 S B -1.0512 Soluble
15 V B -0.3582 Soluble
16 G B -0.9966 Soluble
17 D B -2.0869 Soluble
18 R B -2.4592 Soluble
19 V B 0.0000 Buried
20 T B -0.6200 Soluble
21 I B 0.0000 Buried
22 T B -0.7685 Soluble
23 C B 0.0000 Buried
24 Q B -2.3037 Soluble
25 A B 0.0000 Buried
26 S B -2.2275 Soluble
27 Q B -2.9821 Soluble
28 D B -2.9662 Soluble
29 I B 0.0000 Buried
36 S B -1.5819 Soluble
37 N B -1.3876 Soluble
38 Y B -0.3067 Soluble
39 L B 0.0000 Buried
40 N B 0.0000 Buried
41 W B 0.0000 Buried
42 Y B 0.0000 Buried
43 Q B 0.0000 Buried
44 Q B -1.3224 Soluble
45 K B -1.8760 Soluble
46 P B -1.6131 Soluble
47 G B -1.8618 Soluble
48 K B -2.5632 Soluble
49 A B -1.6742 Soluble
50 P B 0.0000 Buried
51 K B -1.5604 Soluble
52 L B 0.0000 Buried
53 L B 0.0000 Buried
54 I B 0.0000 Buried
55 Y B -0.3154 Soluble
56 D B -0.8574 Soluble
57 A B 0.0000 Buried
65 S B -0.7987 Soluble
66 N B -0.6780 Soluble
67 L B -0.0258 Soluble
68 G B -0.2450 Soluble
69 T B -0.2761 Soluble
70 G B -0.3973 Soluble
71 V B -0.2270 Soluble
72 P B -0.3279 Soluble
74 S B -0.4242 Soluble
75 R B -0.8482 Soluble
76 F B 0.0000 Buried
77 S B -0.3851 Soluble
78 G B -0.4306 Soluble
79 S B -0.9650 Soluble
80 G B -1.2770 Soluble
83 S B -1.6126 Soluble
84 G B -2.0254 Soluble
85 T B -2.3955 Soluble
86 D B -2.6861 Soluble
87 F B 0.0000 Buried
88 T B -0.7487 Soluble
89 F B 0.0000 Buried
90 T B -0.6137 Soluble
91 I B 0.0000 Buried
92 S B -1.4377 Soluble
93 S B -1.2830 Soluble
94 L B 0.0000 Buried
95 Q B -0.9672 Soluble
96 P B -1.5534 Soluble
97 E B -1.8989 Soluble
98 D B 0.0000 Buried
99 I B 0.0000 Buried
100 A B 0.0000 Buried
101 T B -0.8497 Soluble
102 Y B 0.0000 Buried
103 Y B 0.0000 Buried
104 C B 0.0000 Buried
105 Q B 0.0000 Buried
106 Q B 0.0000 Buried
107 A B 0.5880 Aggregation-prone
108 D B 0.6615 Aggregation-prone
109 F B 2.6245 Aggregation-prone
113 V B 2.2540 Aggregation-prone
114 P B 1.0777 Aggregation-prone
115 P B -0.3352 Soluble
116 W B 0.0000 Buried
117 T B -0.8351 Soluble
118 F B 0.0000 Buried
119 G B -0.7806 Soluble
120 G B -1.1175 Soluble
121 G B 0.0000 Buried
122 T B 0.0000 Buried
123 K B -1.5258 Soluble
124 V B 0.0000 Buried
125 E B -1.3417 Soluble
126 I B 0.0000 Buried
127 K B -1.2033 Soluble
128 R B -0.7454 Soluble
129 T B 0.1428 Aggregation-prone
130 V B 1.0082 Aggregation-prone
131 A B 0.3562 Aggregation-prone
132 A B 0.0905 Aggregation-prone
133 P B 0.0000 Buried
134 S B -0.0196 Soluble
135 V B 0.4169 Aggregation-prone
136 F B 0.4503 Aggregation-prone
137 I B 0.6040 Aggregation-prone
138 F B 0.0000 Buried
139 P B -0.2097 Soluble
140 P B -0.2283 Soluble
141 S B -1.2253 Soluble
142 D B -2.4021 Soluble
143 E B -1.9280 Soluble
144 Q B 0.0000 Buried
145 L B -0.4313 Soluble
146 K B -1.9417 Soluble
147 S B -1.2086 Soluble
148 G B -0.8489 Soluble
149 T B -0.2371 Soluble
150 A B 0.3937 Aggregation-prone
151 S B 0.0000 Buried
152 V B 1.0316 Aggregation-prone
153 V B 0.0000 Buried
154 C B 0.0000 Buried
155 L B 0.0000 Buried
156 L B 0.0000 Buried
157 N B 0.0000 Buried
158 N B -0.8788 Soluble
159 F B 0.0000 Buried
160 Y B 0.0000 Buried
161 P B -1.4587 Soluble
162 R B -2.5824 Soluble
163 E B -3.0133 Soluble
164 A B -2.3150 Soluble
165 K B -2.2782 Soluble
166 Q B -1.5917 Soluble
167 E B -2.0607 Soluble
168 S B -0.9078 Soluble
169 V B -0.7292 Soluble
170 T B 0.0000 Buried
171 E B -2.2400 Soluble
172 Q B 0.0000 Buried
173 D B -2.0510 Soluble
174 S B -2.1715 Soluble
175 K B -2.5511 Soluble
176 D B -1.9079 Soluble
177 S B 0.0000 Buried
178 T B -1.3738 Soluble
179 Y B 0.0000 Buried
180 S B 0.0000 Buried
181 L B 0.0000 Buried
182 S B 0.0000 Buried
183 S B -0.4901 Soluble
184 T B 0.0926 Aggregation-prone
185 L B 1.2241 Aggregation-prone
186 T B 0.5809 Aggregation-prone
187 C B -0.4322 Soluble
188 E B -2.0385 Soluble
189 V B -1.1278 Soluble
190 T B -1.2044 Soluble
191 H B 0.0000 Buried
192 Q B -1.2713 Soluble
193 G B -0.3135 Soluble
194 L B -0.0529 Soluble
195 S B -0.5091 Soluble
196 S B -0.3208 Soluble
197 P B -0.3729 Soluble
198 V B 0.0708 Aggregation-prone
199 T B -0.9751 Soluble
200 K B -1.5447 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.4827 5.1323 CSV PDB
4.5 -0.5345 5.0962 CSV PDB
5.0 -0.5956 5.0582 CSV PDB
5.5 -0.6555 5.0196 CSV PDB
6.0 -0.7031 4.9808 CSV PDB
6.5 -0.7303 4.9421 CSV PDB
7.0 -0.7373 4.904 CSV PDB
7.5 -0.7306 4.8673 CSV PDB
8.0 -0.7156 4.8346 CSV PDB
8.5 -0.693 4.8102 CSV PDB
9.0 -0.6617 4.7963 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH