Eramkafusp / 6y90

A4DAB108_6y90_H_L | Source PDB: 6y90 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6861
Max Score
1.7625
Min Score
-3.6604
Total Score
-299.82
Score CDR
-0.2485
Score non-CDR
-0.7388

Calculation Parameters

Chain Sequence(s) QVQLQQPGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGRGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYYCARSTYYGGDWYFNVWGAGTTVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCQIVLSQSPAILSASPGEKVTMTCRASSSVSYIHWFQQKPGSSPKPWIYATSNLASGVPVRFSGSGSGTSYSLTISRVEAEDAATYYCQQWTSNPPTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6604
Maximum Score 1.7625
Average Score -0.6861
Total Score -299.8203

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.4069 Soluble
2 V H -0.9511 Soluble
3 Q H -1.8137 Soluble
4 L H 0.0000 Buried
5 Q H -1.9015 Soluble
6 Q H -1.1965 Soluble
7 P H -1.0475 Soluble
8 G H -1.2389 Soluble
9 A H -1.3860 Soluble
11 E H -1.8148 Soluble
12 L H -0.8245 Soluble
13 V H 0.0000 Buried
14 K H -1.8187 Soluble
15 P H -1.4901 Soluble
16 G H -1.1297 Soluble
17 A H -0.9085 Soluble
18 S H -1.0789 Soluble
19 V H -1.3695 Soluble
20 K H -1.8762 Soluble
21 M H 0.0000 Buried
22 S H -0.8469 Soluble
23 C H 0.0000 Buried
24 K H -1.3674 Soluble
25 A H 0.0000 Buried
26 S H -0.9907 Soluble
27 G H -0.7319 Soluble
28 Y H -0.2338 Soluble
29 T H -0.2048 Soluble
30 F H 0.0000 Buried
35 T H -0.7735 Soluble
36 S H -0.1778 Soluble
37 Y H 0.3839 Aggregation-prone
38 N H 0.0105 Aggregation-prone
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 K H -0.3191 Soluble
44 Q H 0.0000 Buried
45 T H -0.9908 Soluble
46 P H -1.0925 Soluble
47 G H -1.5162 Soluble
48 R H -2.3555 Soluble
49 G H -1.4980 Soluble
50 L H 0.0000 Buried
51 E H -0.9320 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.7713 Soluble
58 P H 0.0000 Buried
59 G H -1.4495 Soluble
62 N H -2.2608 Soluble
63 G H -2.1403 Soluble
64 D H -2.3457 Soluble
65 T H -1.1051 Soluble
66 S H -0.7622 Soluble
67 Y H -1.0593 Soluble
68 N H 0.0000 Buried
69 Q H -2.6537 Soluble
70 K H -2.6600 Soluble
71 F H -1.8522 Soluble
72 K H -2.7003 Soluble
74 G H -1.7755 Soluble
75 K H -1.2773 Soluble
76 A H 0.0000 Buried
77 T H -0.7903 Soluble
78 L H 0.0000 Buried
79 T H -0.6086 Soluble
80 A H -0.9925 Soluble
81 D H -1.6403 Soluble
82 K H -2.3597 Soluble
83 S H -1.2966 Soluble
84 S H -1.0256 Soluble
85 S H -1.1532 Soluble
86 T H -1.0014 Soluble
87 A H 0.0000 Buried
88 Y H -0.4378 Soluble
89 M H 0.0000 Buried
90 Q H -1.3677 Soluble
91 L H 0.0000 Buried
92 S H -0.8719 Soluble
93 S H -0.6172 Soluble
94 L H 0.0000 Buried
95 T H -1.2501 Soluble
96 S H -1.4788 Soluble
97 E H -1.9984 Soluble
98 D H 0.0000 Buried
99 S H -0.7174 Soluble
100 A H 0.0000 Buried
101 V H 0.0819 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 S H 0.0000 Buried
108 T H 0.8713 Aggregation-prone
109 Y H 1.7625 Aggregation-prone
110 Y H 1.4399 Aggregation-prone
111 G H 0.1708 Aggregation-prone
112A G H 0.1879 Aggregation-prone
112 D H 0.5794 Aggregation-prone
113 W H 1.0640 Aggregation-prone
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 N H -0.4006 Soluble
117 V H -0.5300 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 A H -0.5804 Soluble
121 G H -0.3919 Soluble
122 T H 0.0000 Buried
123 T H -0.5278 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.5759 Soluble
128 A H -0.5435 Soluble
129 A H -0.3964 Soluble
130 S H -0.5587 Soluble
131 T H -0.5287 Soluble
132 K H -1.1747 Soluble
133 G H -1.3572 Soluble
134 P H 0.0000 Buried
135 S H -0.5635 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.8340 Soluble
139 L H 0.0000 Buried
140 A H -1.1442 Soluble
141 P H -0.8493 Soluble
142 S H -0.6817 Soluble
143 S H -0.7563 Soluble
144 K H -0.8791 Soluble
145 S H -0.6330 Soluble
146 T H -0.6222 Soluble
147 S H -0.8280 Soluble
148 G H -0.9881 Soluble
149 G H -0.8921 Soluble
150 T H -0.5931 Soluble
151 A H -0.2596 Soluble
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.6125 Soluble
159 D H -1.3216 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.8047 Soluble
164 P H -1.1469 Soluble
165 V H -0.5055 Soluble
166 T H -0.4001 Soluble
167 V H -0.0032 Soluble
168 S H -0.3568 Soluble
169 W H 0.0000 Buried
170 N H -0.6299 Soluble
171 S H -0.6586 Soluble
172 G H -0.5475 Soluble
173 A H -0.2275 Soluble
174 L H 0.0335 Aggregation-prone
175 T H -0.0865 Soluble
176 S H -0.2607 Soluble
177 G H -0.3395 Soluble
178 V H 0.3544 Aggregation-prone
179 H H -0.3023 Soluble
180 T H -0.1630 Soluble
181 F H 0.0000 Buried
182 P H -0.3578 Soluble
183 A H 0.2779 Aggregation-prone
184 V H 0.6668 Aggregation-prone
185 L H 1.1412 Aggregation-prone
186 Q H -0.3066 Soluble
187 S H -0.2957 Soluble
188 S H -0.3370 Soluble
189 G H -0.1154 Soluble
190 L H -0.2869 Soluble
191 Y H 0.0000 Buried
192 S H -0.1060 Soluble
193 L H 0.1242 Aggregation-prone
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.0209 Soluble
199 V H 0.0000 Buried
200 P H -0.4792 Soluble
201 S H -0.5942 Soluble
202 S H -0.6154 Soluble
203 S H -0.5383 Soluble
204 L H -0.7618 Soluble
205 G H -0.9679 Soluble
206 T H -0.6714 Soluble
207 Q H -0.9066 Soluble
208 T H -0.8513 Soluble
209 Y H 0.0000 Buried
210 I H -0.8703 Soluble
211 C H 0.0000 Buried
212 N H -1.5960 Soluble
213 V H 0.0000 Buried
214 N H -2.0389 Soluble
215 H H 0.0000 Buried
216 K H -2.8927 Soluble
217 P H -1.8695 Soluble
218 S H -1.8180 Soluble
219 N H -2.5745 Soluble
220 T H -2.0322 Soluble
221 K H -2.8689 Soluble
222 V H -1.6836 Soluble
223 D H -2.5678 Soluble
224 K H -1.8630 Soluble
225 K H -1.7666 Soluble
226 V H 0.0000 Buried
227 E H -2.4848 Soluble
228 P H -1.8624 Soluble
229 K H -2.1669 Soluble
230 S H -1.0096 Soluble
231 C H -0.3339 Soluble
1 Q L -1.1626 Soluble
2 I L 0.1851 Aggregation-prone
3 V L 1.2380 Aggregation-prone
4 L L 0.0000 Buried
5 S L -0.6252 Soluble
6 Q L -1.0384 Soluble
7 S L -0.3946 Soluble
8 P L 0.1110 Aggregation-prone
9 A L 0.4041 Aggregation-prone
10 I L 1.3548 Aggregation-prone
11 L L 0.4219 Aggregation-prone
12 S L -0.6341 Soluble
13 A L 0.0000 Buried
14 S L -2.6596 Soluble
15 P L -2.9066 Soluble
16 G L -2.8075 Soluble
17 E L -3.6604 Soluble
18 K L -3.2440 Soluble
19 V L -1.4991 Soluble
20 T L -0.3360 Soluble
21 M L 0.0000 Buried
22 T L -0.7416 Soluble
23 C L 0.0000 Buried
24 R L -1.9722 Soluble
25 A L 0.0000 Buried
26 S L -0.3316 Soluble
27 S L -0.4621 Soluble
28 S L -0.6513 Soluble
29 V L 0.0000 Buried
37 S L -0.0909 Soluble
38 Y L 0.3309 Aggregation-prone
39 I L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 F L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.4143 Soluble
45 K L -2.3350 Soluble
46 P L -1.6455 Soluble
47 G L -1.0758 Soluble
48 S L -1.1698 Soluble
49 S L -0.8863 Soluble
50 P L 0.0000 Buried
51 K L -1.4756 Soluble
52 P L -0.6699 Soluble
53 W L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 A L -0.1602 Soluble
57 T L -0.3795 Soluble
65 S L -0.7362 Soluble
66 N L -1.0012 Soluble
67 L L 0.0972 Aggregation-prone
68 A L -0.3181 Soluble
69 S L -0.4006 Soluble
70 G L -0.1497 Soluble
71 V L 0.5201 Aggregation-prone
72 P L 0.8050 Aggregation-prone
74 V L 1.4834 Aggregation-prone
75 R L -0.0951 Soluble
76 F L 0.0000 Buried
77 S L -0.1133 Soluble
78 G L -0.4882 Soluble
79 S L -0.6908 Soluble
80 G L -0.6227 Soluble
83 S L -0.6189 Soluble
84 G L -0.8395 Soluble
85 T L -0.9980 Soluble
86 S L -1.2557 Soluble
87 Y L 0.0000 Buried
88 S L -0.6064 Soluble
89 L L 0.0000 Buried
90 T L -0.7387 Soluble
91 I L 0.0000 Buried
92 S L -1.9851 Soluble
93 R L -3.3977 Soluble
94 V L 0.0000 Buried
95 E L -2.8290 Soluble
96 A L 0.0000 Buried
97 E L -2.5751 Soluble
98 D L 0.0000 Buried
99 A L -1.8084 Soluble
100 A L -1.5670 Soluble
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 W L 0.0500 Aggregation-prone
108 T L -0.3169 Soluble
109 S L -0.9336 Soluble
114 N L -1.5357 Soluble
115 P L 0.0000 Buried
116 P L 0.0000 Buried
117 T L -0.1396 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.1148 Soluble
121 G L -0.4605 Soluble
122 T L 0.0000 Buried
123 K L -0.8868 Soluble
124 L L 0.0000 Buried
125 E L -1.1218 Soluble
126 I L 0.0000 Buried
127 K L -2.4449 Soluble
128 R L -2.3354 Soluble
129 T L -0.5473 Soluble
130 V L 0.6790 Aggregation-prone
131 A L 0.3135 Aggregation-prone
132 A L 0.0463 Aggregation-prone
133 P L 0.0000 Buried
134 S L 0.1689 Aggregation-prone
135 V L 0.0000 Buried
136 F L 0.5344 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L 0.0000 Buried
140 P L -0.4642 Soluble
141 S L -1.1759 Soluble
142 D L -2.3731 Soluble
143 E L -1.9864 Soluble
144 Q L 0.0000 Buried
145 L L -1.5681 Soluble
146 K L -2.3095 Soluble
147 S L -1.4217 Soluble
148 G L -1.0393 Soluble
149 T L -0.6931 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.4180 Soluble
158 N L -0.4868 Soluble
159 F L 0.0000 Buried
160 Y L -0.8336 Soluble
161 P L -1.2130 Soluble
162 R L -1.9863 Soluble
163 E L -2.8428 Soluble
164 A L -2.0634 Soluble
165 K L -2.2564 Soluble
166 V L -1.3153 Soluble
167 Q L -0.8649 Soluble
168 W L 0.0000 Buried
169 K L -1.0296 Soluble
170 V L 0.0000 Buried
171 D L -2.5030 Soluble
172 N L -1.8741 Soluble
173 A L -0.8312 Soluble
174 L L 0.2940 Aggregation-prone
175 Q L -0.4584 Soluble
176 S L -0.8232 Soluble
177 G L -1.5360 Soluble
178 N L -1.8721 Soluble
179 S L -1.7119 Soluble
180 Q L -2.0535 Soluble
181 E L -1.6178 Soluble
182 S L -0.4130 Soluble
183 V L -0.0589 Soluble
184 T L -0.8595 Soluble
185 E L -2.1590 Soluble
186 Q L -1.6284 Soluble
187 D L -2.3030 Soluble
188 S L -2.5996 Soluble
189 K L -3.0671 Soluble
190 D L -2.9062 Soluble
191 S L 0.0000 Buried
192 T L -1.7607 Soluble
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L -0.5224 Soluble
197 S L 0.0000 Buried
198 T L -0.7345 Soluble
199 L L 0.0000 Buried
200 T L -0.5562 Soluble
201 L L -0.5370 Soluble
202 S L -1.2116 Soluble
203 K L -1.8222 Soluble
204 A L -1.8172 Soluble
205 D L -2.4127 Soluble
206 Y L 0.0000 Buried
207 E L -3.4953 Soluble
208 K L -3.4263 Soluble
209 H L -2.5223 Soluble
210 K L -2.4481 Soluble
211 V L -0.5193 Soluble
212 Y L 0.0000 Buried
213 A L -0.4632 Soluble
214 C L 0.0000 Buried
215 E L -0.5606 Soluble
216 V L 0.0000 Buried
217 T L -1.0429 Soluble
218 H L 0.0000 Buried
219 Q L -1.3041 Soluble
220 G L -0.3693 Soluble
221 L L -0.1552 Soluble
222 S L -0.4659 Soluble
223 S L -0.2928 Soluble
224 P L -0.1409 Soluble
225 V L 0.5934 Aggregation-prone
226 T L 0.1790 Aggregation-prone
227 K L -0.1330 Soluble
228 S L -0.1400 Soluble
229 F L 0.0000 Buried
230 N L -0.8427 Soluble
231 R L -1.6486 Soluble
232 G L -1.8153 Soluble
233 E L -2.1244 Soluble
234 C L -1.1026 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7499 2.5208 CSV PDB
4.5 -0.7875 2.5043 CSV PDB
5.0 -0.8327 2.4858 CSV PDB
5.5 -0.8741 2.4666 CSV PDB
6.0 -0.8987 2.4471 CSV PDB
6.5 -0.8971 2.4275 CSV PDB
7.0 -0.8693 2.408 CSV PDB
7.5 -0.8243 2.3886 CSV PDB
8.0 -0.7707 2.5363 CSV PDB
8.5 -0.712 2.6979 CSV PDB
9.0 -0.6492 2.8606 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH