Sutacimig / 8cn9

A4DAB249_8cn9_G_F | Source PDB: 8cn9 | Chains: G, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6389
Max Score
1.4816
Min Score
-3.4837
Total Score
-271.54
Score CDR
-0.4138
Score non-CDR
-0.6683

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSQTLSLTCTVSGYSISSDSAWSWIRQPPGKGLEWIGYIQYSGSTNYNPSLKSRVTISRDTSKNQFSLKLSSVTAADTAVYYCARSVNYYGNSFAVGYWGQGTLVTVSSASTKGPSVFPLAPCSRSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVEIVLTQSPATLSLSPGERATLSCRASQGISDYLHWYQQKPGEAPRLLIKYTSQPATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQNGHSFPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSF input.pdb
Selected Chain(s) G (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4837
Maximum Score 1.4816
Average Score -0.6389
Total Score -271.5388

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q G -1.3600 Soluble
2 V G -0.8352 Soluble
3 Q G -1.5772 Soluble
4 L G 0.0000 Buried
5 Q G -1.8471 Soluble
6 E G -1.1364 Soluble
7 S G -0.7125 Soluble
8 G G -0.5158 Soluble
9 P G -0.4313 Soluble
11 G G 0.0000 Buried
12 L G -0.2113 Soluble
13 V G 0.0000 Buried
14 K G -1.4307 Soluble
15 P G -1.1361 Soluble
16 S G -1.2099 Soluble
17 Q G -1.7263 Soluble
18 T G -1.3756 Soluble
19 L G 0.0000 Buried
20 S G -0.8998 Soluble
21 L G 0.0000 Buried
22 T G -0.7010 Soluble
23 C G 0.0000 Buried
24 T G -1.1120 Soluble
25 V G 0.0000 Buried
26 S G -1.1013 Soluble
27 G G -0.8756 Soluble
28 Y G -0.6028 Soluble
29 S G -0.6773 Soluble
30 I G 0.0000 Buried
31 S G -0.7662 Soluble
35 S G -1.0123 Soluble
36 D G -1.6791 Soluble
37 S G 0.0000 Buried
38 A G 0.0000 Buried
39 W G 0.0000 Buried
40 S G 0.0000 Buried
41 W G 0.0000 Buried
42 I G 0.0000 Buried
43 R G 0.0000 Buried
44 Q G 0.0000 Buried
45 P G -0.5379 Soluble
46 P G -0.6730 Soluble
47 G G -1.0696 Soluble
48 K G -1.2117 Soluble
49 G G -1.0638 Soluble
50 L G 0.0000 Buried
51 E G -0.5728 Soluble
52 W G 0.0000 Buried
53 I G 0.0000 Buried
54 G G 0.0000 Buried
55 Y G 0.0000 Buried
56 I G 0.0000 Buried
57 Q G -0.7301 Soluble
58 Y G -0.5607 Soluble
59 S G -0.5542 Soluble
63 G G -0.6244 Soluble
64 S G -0.6156 Soluble
65 T G -0.6788 Soluble
66 N G -1.2108 Soluble
67 Y G -0.9843 Soluble
68 N G 0.0000 Buried
69 P G -1.2610 Soluble
70 S G -0.9795 Soluble
71 L G 0.0000 Buried
72 K G -2.1196 Soluble
74 S G -1.2706 Soluble
75 R G -1.3527 Soluble
76 V G 0.0000 Buried
77 T G -1.1223 Soluble
78 I G 0.0000 Buried
79 S G -0.9754 Soluble
80 R G -1.3850 Soluble
81 D G -1.7998 Soluble
82 T G -1.3549 Soluble
83 S G -1.5014 Soluble
84 K G -2.3605 Soluble
85 N G -1.6432 Soluble
86 Q G -1.4755 Soluble
87 F G 0.0000 Buried
88 S G -0.8077 Soluble
89 L G 0.0000 Buried
90 K G -1.6683 Soluble
91 L G 0.0000 Buried
92 S G -1.1941 Soluble
93 S G -0.9770 Soluble
94 V G 0.0000 Buried
95 T G -0.4495 Soluble
96 A G -0.1241 Soluble
97 A G 0.0524 Aggregation-prone
98 D G 0.0000 Buried
99 T G -0.0067 Soluble
100 A G 0.0000 Buried
101 V G 0.0478 Aggregation-prone
102 Y G 0.0000 Buried
103 Y G 0.0000 Buried
104 C G 0.0000 Buried
105 A G 0.0000 Buried
106 R G 0.0000 Buried
107 S G 0.0000 Buried
108 V G 0.0425 Aggregation-prone
109 N G -0.2244 Soluble
110 Y G -0.0545 Soluble
111 Y G 0.6136 Aggregation-prone
111A G G -0.4644 Soluble
112A N G -1.0902 Soluble
112 S G -0.3984 Soluble
113 F G 0.0000 Buried
114 A G 0.0000 Buried
115 V G 0.0000 Buried
116 G G 0.0106 Aggregation-prone
117 Y G 0.2398 Aggregation-prone
118 W G -0.5492 Soluble
119 G G 0.0000 Buried
120 Q G -2.0795 Soluble
121 G G 0.0000 Buried
122 T G 0.0000 Buried
123 L G -0.1389 Soluble
124 V G 0.0000 Buried
125 T G 0.0000 Buried
126 V G 0.0000 Buried
127 S G -0.4773 Soluble
128 S G -0.5513 Soluble
129 A G -0.5241 Soluble
130 S G -0.7143 Soluble
131 T G -0.9647 Soluble
132 K G -1.9179 Soluble
133 G G -1.8434 Soluble
134 P G -1.1610 Soluble
135 S G -0.5556 Soluble
136 V G 0.0000 Buried
137 F G -0.5093 Soluble
138 P G -0.7119 Soluble
139 L G 0.0000 Buried
140 A G 0.2843 Aggregation-prone
141 P G -0.0053 Soluble
142 C G 0.3680 Aggregation-prone
143 S G -0.7202 Soluble
144 R G -1.8876 Soluble
145 S G -1.1529 Soluble
146 T G -0.2803 Soluble
147 A G -0.3881 Soluble
148 A G 0.0000 Buried
149 L G 0.0000 Buried
150 G G 0.0000 Buried
151 C G 0.0000 Buried
152 L G 0.0000 Buried
153 V G 0.0000 Buried
154 K G 0.0000 Buried
155 D G -1.1153 Soluble
156 Y G 0.0000 Buried
157 F G 0.0000 Buried
158 P G 0.0000 Buried
159 E G -0.5179 Soluble
160 P G -0.6255 Soluble
161 V G 0.0000 Buried
162 T G -0.5743 Soluble
163 V G 0.0000 Buried
164 S G -0.6456 Soluble
165 W G 0.0000 Buried
166 N G -0.9505 Soluble
167 S G -0.7416 Soluble
168 G G -0.6142 Soluble
169 A G -0.2354 Soluble
170 L G -0.0255 Soluble
171 T G -0.1572 Soluble
172 S G -0.1804 Soluble
173 G G -0.0891 Soluble
174 V G 0.2462 Aggregation-prone
175 H G -0.1374 Soluble
176 T G -0.0104 Soluble
177 F G 0.0000 Buried
178 P G 0.0041 Aggregation-prone
179 A G 0.5596 Aggregation-prone
180 V G 0.9959 Aggregation-prone
181 L G 1.4816 Aggregation-prone
182 Q G 0.3800 Aggregation-prone
183 S G -0.1067 Soluble
184 S G -0.2960 Soluble
185 G G -0.0396 Soluble
186 L G -0.1207 Soluble
187 Y G 0.3369 Aggregation-prone
188 S G 0.2248 Aggregation-prone
189 L G 0.0000 Buried
190 S G 0.0000 Buried
191 S G 0.0000 Buried
192 V G 0.0000 Buried
193 V G 0.0000 Buried
194 T G -0.0836 Soluble
195 V G 0.0000 Buried
196 P G -0.3133 Soluble
197 S G -0.5806 Soluble
198 S G -0.4614 Soluble
199 S G -0.5234 Soluble
200 L G -0.3844 Soluble
201 G G -0.6081 Soluble
202 T G -0.7292 Soluble
203 K G -1.1490 Soluble
204 T G -0.8744 Soluble
205 Y G 0.0000 Buried
206 T G -1.5784 Soluble
207 C G 0.0000 Buried
208 N G -1.8342 Soluble
209 V G 0.0000 Buried
210 D G -1.9704 Soluble
211 H G 0.0000 Buried
212 K G -1.7430 Soluble
213 P G -1.2204 Soluble
214 S G -1.7248 Soluble
215 N G -2.0873 Soluble
216 T G -1.9734 Soluble
217 K G -2.7653 Soluble
218 V G -1.9193 Soluble
219 D G -2.9699 Soluble
220 K G -2.4637 Soluble
221 R G -2.3212 Soluble
222 V G -0.4881 Soluble
1 E F -1.5324 Soluble
2 I F 0.0000 Buried
3 V F 0.6451 Aggregation-prone
4 L F 0.0000 Buried
5 T F -0.7668 Soluble
6 Q F 0.0000 Buried
7 S F -0.8468 Soluble
8 P F -0.5234 Soluble
9 A F -0.6093 Soluble
10 T F -0.6026 Soluble
11 L F -0.3570 Soluble
12 S F -0.4984 Soluble
13 L F -1.1180 Soluble
14 S F -1.6610 Soluble
15 P F -1.9500 Soluble
16 G F -1.9275 Soluble
17 E F -2.7614 Soluble
18 R F -2.8663 Soluble
19 A F 0.0000 Buried
20 T F -0.5402 Soluble
21 L F 0.0000 Buried
22 S F -1.0426 Soluble
23 C F 0.0000 Buried
24 R F -2.5540 Soluble
25 A F 0.0000 Buried
26 S F -1.2142 Soluble
27 Q F -1.9931 Soluble
28 G F -1.6987 Soluble
29 I F 0.0000 Buried
36 S F -0.9398 Soluble
37 D F -0.7639 Soluble
38 Y F 0.2578 Aggregation-prone
39 L F 0.0000 Buried
40 H F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F 0.0000 Buried
45 K F -1.5140 Soluble
46 P F -1.3699 Soluble
47 G F -1.6432 Soluble
48 E F -2.5698 Soluble
49 A F -1.5062 Soluble
50 P F 0.0000 Buried
51 R F -1.0796 Soluble
52 L F -0.3372 Soluble
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 K F -0.6487 Soluble
56 Y F -0.3278 Soluble
57 T F 0.0000 Buried
65 S F -1.0863 Soluble
66 Q F -1.3867 Soluble
67 P F -0.8492 Soluble
68 A F -0.6577 Soluble
69 T F -0.5407 Soluble
70 G F -0.4188 Soluble
71 I F -0.3806 Soluble
72 P F -0.2569 Soluble
74 A F -0.2048 Soluble
75 R F -0.6701 Soluble
76 F F 0.0000 Buried
77 S F -0.5467 Soluble
78 G F -0.6120 Soluble
79 S F -0.8926 Soluble
80 G F -1.0377 Soluble
83 S F -1.0469 Soluble
84 G F -1.5050 Soluble
85 T F -2.1040 Soluble
86 D F -2.6956 Soluble
87 F F 0.0000 Buried
88 T F -0.8655 Soluble
89 L F 0.0000 Buried
90 T F -0.6508 Soluble
91 I F 0.0000 Buried
92 S F -1.5848 Soluble
93 S F -1.7285 Soluble
94 L F 0.0000 Buried
95 E F -2.0700 Soluble
96 P F -2.0683 Soluble
97 E F -2.3351 Soluble
98 D F 0.0000 Buried
99 F F -1.2250 Soluble
100 A F 0.0000 Buried
101 V F 0.0000 Buried
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 N F 0.0000 Buried
107 G F 0.0000 Buried
108 H F -0.7352 Soluble
109 S F -0.7851 Soluble
114 F F -0.5631 Soluble
115 P F -0.7421 Soluble
116 L F 0.0000 Buried
117 T F -0.1940 Soluble
118 F F 0.0000 Buried
119 G F 0.0000 Buried
120 Q F -1.3210 Soluble
121 G F 0.0000 Buried
122 T F 0.0000 Buried
123 K F -1.5715 Soluble
124 L F 0.0000 Buried
125 E F -1.0067 Soluble
126 I F 0.0000 Buried
127 K F -1.5312 Soluble
128 R F -1.2467 Soluble
129 T F -0.3411 Soluble
130 V F 0.1759 Aggregation-prone
131 A F -0.0106 Soluble
132 A F -0.1415 Soluble
133 P F -0.1963 Soluble
134 S F -0.0711 Soluble
135 V F 0.2276 Aggregation-prone
136 F F 0.2707 Aggregation-prone
137 I F 0.3964 Aggregation-prone
138 F F 0.0000 Buried
139 P F 0.0730 Aggregation-prone
140 P F -0.5900 Soluble
141 S F -1.5599 Soluble
142 D F -2.8617 Soluble
143 E F -3.0175 Soluble
144 Q F 0.0000 Buried
145 L F -2.0216 Soluble
146 K F -2.7084 Soluble
147 S F -1.4656 Soluble
148 G F -1.0575 Soluble
149 T F -0.8937 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.5636 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F 0.0000 Buried
162 R F -2.7956 Soluble
163 E F -3.1278 Soluble
164 A F -2.3696 Soluble
165 K F -2.3893 Soluble
166 V F -1.1063 Soluble
167 Q F -0.6863 Soluble
168 W F 0.0000 Buried
169 K F -0.5886 Soluble
170 V F -1.1235 Soluble
171 D F -2.4665 Soluble
172 N F -1.7130 Soluble
173 A F -0.5322 Soluble
174 L F 0.7274 Aggregation-prone
175 Q F -0.2395 Soluble
176 S F -0.5306 Soluble
177 G F -1.0705 Soluble
178 N F -1.2582 Soluble
179 S F -1.0435 Soluble
180 Q F -0.7440 Soluble
181 E F -0.9568 Soluble
182 S F -0.1016 Soluble
183 V F -0.1206 Soluble
184 T F -0.8256 Soluble
185 E F -1.8707 Soluble
186 Q F 0.0000 Buried
187 D F -1.9363 Soluble
188 S F -2.2134 Soluble
189 K F -2.5806 Soluble
190 D F -1.9435 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.4133 Soluble
199 L F 0.0000 Buried
200 T F -0.4706 Soluble
201 L F -0.6337 Soluble
202 S F -1.0944 Soluble
203 K F -2.0623 Soluble
204 A F -1.6699 Soluble
205 D F -2.2236 Soluble
206 Y F 0.0000 Buried
207 E F -3.4837 Soluble
208 K F -3.4700 Soluble
209 H F -2.4772 Soluble
210 K F -2.1439 Soluble
211 V F -0.6403 Soluble
212 Y F 0.0000 Buried
213 A F -0.6953 Soluble
214 C F 0.0000 Buried
215 E F -0.8840 Soluble
216 V F 0.0000 Buried
217 T F -1.0601 Soluble
218 H F 0.0000 Buried
219 Q F -1.4453 Soluble
220 G F -0.5544 Soluble
221 L F -0.2622 Soluble
222 S F -0.4493 Soluble
223 S F -0.3521 Soluble
224 P F -0.2617 Soluble
225 V F 0.3118 Aggregation-prone
226 T F -0.2310 Soluble
227 K F -0.3670 Soluble
228 S F -0.0108 Soluble
229 F F 0.7225 Aggregation-prone

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6625 1.9035 CSV PDB
4.5 -0.7002 1.9035 CSV PDB
5.0 -0.7455 1.9035 CSV PDB
5.5 -0.7892 1.9035 CSV PDB
6.0 -0.8209 1.9035 CSV PDB
6.5 -0.8329 1.9035 CSV PDB
7.0 -0.8251 1.9035 CSV PDB
7.5 -0.8039 1.9035 CSV PDB
8.0 -0.7753 1.9262 CSV PDB
8.5 -0.7414 1.9827 CSV PDB
9.0 -0.702 2.0911 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH