Izalontamab / 6azk

A4DAB149_6azk_D_C | Source PDB: 6azk | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6388
Max Score
2.4138
Min Score
-3.4674
Total Score
-275.98
Score CDR
-0.2932
Score non-CDR
-0.68

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4674
Maximum Score 2.4138
Average Score -0.6388
Total Score -275.978

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.4531 Soluble
2 V D -0.8499 Soluble
3 Q D -1.5177 Soluble
4 L D 0.0000 Buried
5 K D -2.1312 Soluble
6 Q D 0.0000 Buried
7 S D -0.7058 Soluble
8 G D -0.9245 Soluble
9 P D -0.3720 Soluble
11 G D -0.3545 Soluble
12 L D 0.0567 Aggregation-prone
13 V D -0.6200 Soluble
14 Q D -1.2642 Soluble
15 P D -1.5270 Soluble
16 S D -1.5509 Soluble
17 Q D -1.9191 Soluble
18 S D -1.4895 Soluble
19 L D 0.0000 Buried
20 S D -0.4986 Soluble
21 I D 0.0000 Buried
22 T D -0.4274 Soluble
23 C D 0.0000 Buried
24 T D -1.0817 Soluble
25 V D 0.0000 Buried
26 S D -1.1846 Soluble
27 G D -0.9791 Soluble
28 F D -0.5455 Soluble
29 S D -0.8911 Soluble
30 L D 0.0000 Buried
35 T D -0.9119 Soluble
36 N D -0.8483 Soluble
37 Y D 0.4454 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4187 Soluble
45 S D -0.9683 Soluble
46 P D -1.1570 Soluble
47 G D -1.7324 Soluble
48 K D -2.2271 Soluble
49 G D -1.1215 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5271 Soluble
58 S D -0.6636 Soluble
59 G D -1.2235 Soluble
63 G D -1.5191 Soluble
64 N D -1.8367 Soluble
65 T D -1.2843 Soluble
66 D D -0.9897 Soluble
67 Y D -0.4070 Soluble
68 N D -0.2118 Soluble
69 T D -0.2514 Soluble
70 P D -0.3994 Soluble
71 F D 0.0000 Buried
72 T D -0.4720 Soluble
74 S D -0.5571 Soluble
75 R D -0.9015 Soluble
76 L D 0.0000 Buried
77 S D -0.8990 Soluble
78 I D 0.0000 Buried
79 N D -1.6777 Soluble
80 K D -1.4284 Soluble
81 D D -1.7963 Soluble
82 N D -2.2575 Soluble
83 S D -1.7018 Soluble
84 K D -2.3849 Soluble
85 S D -1.6304 Soluble
86 Q D -1.5248 Soluble
87 V D 0.0000 Buried
88 F D -0.1749 Soluble
89 F D 0.0000 Buried
90 K D -1.1868 Soluble
91 M D 0.0000 Buried
92 N D -1.5331 Soluble
93 S D -1.4560 Soluble
94 L D 0.0000 Buried
95 Q D -2.1151 Soluble
96 S D -1.5200 Soluble
97 N D -2.0844 Soluble
98 D D 0.0000 Buried
99 T D -0.6009 Soluble
100 A D 0.0000 Buried
101 I D 0.5847 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0889 Aggregation-prone
109 T D 1.2040 Aggregation-prone
110 Y D 1.8790 Aggregation-prone
111 Y D 1.9839 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.6247 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.2624 Aggregation-prone
117 Y D 0.1853 Aggregation-prone
118 W D -0.4693 Soluble
119 G D 0.0000 Buried
120 Q D -1.7642 Soluble
121 G D -0.8395 Soluble
122 T D 0.0000 Buried
123 L D 0.3276 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.2355 Soluble
126 V D 0.0000 Buried
127 S D -0.5841 Soluble
128 A D -0.3629 Soluble
129 A D -0.2536 Soluble
130 S D -0.4665 Soluble
131 T D -0.5519 Soluble
132 K D -1.1689 Soluble
133 G D -1.3751 Soluble
134 P D -0.6420 Soluble
135 S D -0.3974 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D 0.0000 Buried
139 L D 0.0000 Buried
140 A D -1.2423 Soluble
141 P D 0.0000 Buried
142 S D -1.1309 Soluble
143 S D -0.7768 Soluble
144 K D -1.1977 Soluble
145 S D 0.0000 Buried
146 T D -0.7854 Soluble
147 S D -0.7971 Soluble
148 G D -0.8377 Soluble
149 G D -0.9076 Soluble
150 T D -0.6354 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D -0.2582 Soluble
159 D D -0.3813 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.2456 Soluble
164 P D -0.5275 Soluble
165 V D -0.6674 Soluble
166 T D -0.6252 Soluble
167 V D -0.2520 Soluble
168 S D -0.4219 Soluble
169 W D 0.0000 Buried
170 N D -0.7968 Soluble
171 S D -0.6779 Soluble
172 G D -0.5262 Soluble
173 A D -0.2333 Soluble
174 L D 0.0003 Aggregation-prone
175 T D -0.1602 Soluble
176 S D -0.1683 Soluble
177 G D -0.1968 Soluble
178 V D 0.1898 Aggregation-prone
179 H D -0.2574 Soluble
180 T D 0.0255 Aggregation-prone
181 F D 0.0000 Buried
182 P D -0.1804 Soluble
183 A D 0.3465 Aggregation-prone
184 V D 0.6528 Aggregation-prone
185 L D 1.3477 Aggregation-prone
186 Q D 0.3665 Aggregation-prone
187 S D -0.0162 Soluble
188 S D -0.1686 Soluble
189 G D 0.1149 Aggregation-prone
190 L D 0.2236 Aggregation-prone
191 Y D 0.5236 Aggregation-prone
192 S D 0.0000 Buried
193 L D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.1180 Soluble
199 V D 0.0000 Buried
200 P D -0.5425 Soluble
201 S D -0.6005 Soluble
202 S D -0.5782 Soluble
203 S D -0.6073 Soluble
204 L D -0.8536 Soluble
205 G D -0.9902 Soluble
206 T D -0.6899 Soluble
207 Q D -1.1553 Soluble
208 T D -1.1467 Soluble
209 Y D 0.0000 Buried
210 I D -1.5419 Soluble
211 C D 0.0000 Buried
212 N D -1.6115 Soluble
213 V D 0.0000 Buried
214 N D -2.1323 Soluble
215 H D 0.0000 Buried
216 K D -2.6940 Soluble
217 P D -1.5342 Soluble
218 S D -1.8089 Soluble
219 N D -2.5836 Soluble
220 T D -2.1053 Soluble
221 K D -2.8170 Soluble
222 V D -1.7615 Soluble
223 D D -2.7059 Soluble
224 K D -2.4375 Soluble
225 R D -2.7894 Soluble
226 V D 0.0000 Buried
227 E D -2.8991 Soluble
228 P D -1.8923 Soluble
229 K D -2.2423 Soluble
1 D C -1.2345 Soluble
2 I C -0.3093 Soluble
3 L C 0.7191 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.2608 Soluble
6 Q C 0.0000 Buried
7 S C 0.3971 Aggregation-prone
8 P C 1.2031 Aggregation-prone
9 V C 2.4138 Aggregation-prone
10 I C 2.3761 Aggregation-prone
11 L C 1.1448 Aggregation-prone
12 S C 0.1695 Aggregation-prone
13 V C -1.0413 Soluble
14 S C -1.7590 Soluble
15 P C -1.9487 Soluble
16 G C -2.1660 Soluble
17 E C -3.0512 Soluble
18 R C -3.2517 Soluble
19 V C 0.0000 Buried
20 S C -0.9471 Soluble
21 F C 0.0000 Buried
22 S C -0.7625 Soluble
23 C C 0.0000 Buried
24 R C -2.4014 Soluble
25 A C -1.2322 Soluble
26 S C -0.9123 Soluble
27 Q C -1.2755 Soluble
28 S C -1.4926 Soluble
29 I C 0.0000 Buried
36 G C -1.0623 Soluble
37 T C -0.8231 Soluble
38 N C -0.8257 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.2276 Soluble
45 R C -1.9728 Soluble
46 T C -1.7140 Soluble
47 N C -1.9821 Soluble
48 G C -1.5387 Soluble
49 S C -0.9710 Soluble
50 P C 0.0000 Buried
51 R C -0.6457 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7566 Soluble
56 Y C -0.5711 Soluble
57 A C 0.0000 Buried
65 S C -1.1872 Soluble
66 E C -1.8087 Soluble
67 S C -1.1056 Soluble
68 I C -0.4869 Soluble
69 S C -0.3408 Soluble
70 G C -0.4744 Soluble
71 I C -0.2499 Soluble
72 P C -0.3215 Soluble
74 S C -0.5568 Soluble
75 R C -1.0287 Soluble
76 F C 0.0000 Buried
77 S C -0.8821 Soluble
78 G C -0.7453 Soluble
79 S C -0.8734 Soluble
80 G C -1.1645 Soluble
83 S C -1.2234 Soluble
84 G C -1.3480 Soluble
85 T C -1.9217 Soluble
86 D C -2.5444 Soluble
87 F C 0.0000 Buried
88 T C -0.9119 Soluble
89 L C 0.0000 Buried
90 S C -0.8989 Soluble
91 I C 0.0000 Buried
92 N C -2.3247 Soluble
93 S C -2.2560 Soluble
94 V C 0.0000 Buried
95 E C -2.4362 Soluble
96 S C -2.2050 Soluble
97 E C -2.1913 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C 0.0000 Buried
101 D C -0.5113 Soluble
102 Y C 0.0000 Buried
103 Y C -0.0848 Soluble
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6064 Soluble
108 N C -1.2616 Soluble
109 N C -0.9694 Soluble
114 W C 0.1124 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C 0.0306 Aggregation-prone
118 F C 0.2772 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.1315 Aggregation-prone
121 G C 0.3912 Aggregation-prone
122 T C 0.0000 Buried
123 K C 0.5888 Aggregation-prone
124 L C 0.0000 Buried
125 E C -0.4369 Soluble
126 L C 0.0000 Buried
127 K C -1.4936 Soluble
128 R C -1.0415 Soluble
129 T C 0.1775 Aggregation-prone
130 V C 1.0677 Aggregation-prone
131 A C 0.3465 Aggregation-prone
132 A C 0.0597 Aggregation-prone
133 P C 0.0000 Buried
134 S C -0.1972 Soluble
135 V C 0.0000 Buried
136 F C 0.0000 Buried
137 I C 0.0000 Buried
138 F C 0.0000 Buried
139 P C -0.7269 Soluble
140 P C -0.7454 Soluble
141 S C 0.0000 Buried
142 D C -2.6618 Soluble
143 E C -2.3964 Soluble
144 Q C 0.0000 Buried
145 L C -2.1263 Soluble
146 K C -2.6561 Soluble
147 S C -1.6314 Soluble
148 G C -1.1778 Soluble
149 T C -0.9542 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.8686 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.2130 Soluble
162 R C -1.9975 Soluble
163 E C -2.7648 Soluble
164 A C -2.1037 Soluble
165 K C -2.3302 Soluble
166 V C 0.0000 Buried
167 Q C -0.9118 Soluble
168 W C 0.0000 Buried
169 K C -0.8757 Soluble
170 V C 0.0000 Buried
171 D C -2.0365 Soluble
172 N C -1.6513 Soluble
173 A C -0.5166 Soluble
174 L C 0.2033 Aggregation-prone
175 Q C -0.3588 Soluble
176 S C -0.5151 Soluble
177 G C -0.8843 Soluble
178 N C -0.7975 Soluble
179 S C -1.0466 Soluble
180 Q C -1.1300 Soluble
181 E C -1.5144 Soluble
182 S C -0.6225 Soluble
183 V C -0.5409 Soluble
184 T C -0.8495 Soluble
185 E C -1.9890 Soluble
186 Q C 0.0000 Buried
187 D C -1.9365 Soluble
188 S C -2.2904 Soluble
189 K C -2.6301 Soluble
190 D C -1.9173 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.5737 Soluble
199 L C 0.0000 Buried
200 T C -0.4435 Soluble
201 L C -0.6506 Soluble
202 S C -1.1998 Soluble
203 K C -1.9873 Soluble
204 A C -1.8320 Soluble
205 D C -2.4240 Soluble
206 Y C 0.0000 Buried
207 E C -3.1922 Soluble
208 K C -3.4674 Soluble
209 H C -2.9225 Soluble
210 K C -3.0419 Soluble
211 V C -1.6002 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9028 Soluble
216 V C 0.0000 Buried
217 T C -1.1740 Soluble
218 H C 0.0000 Buried
219 Q C -1.5012 Soluble
220 G C -0.2061 Soluble
221 L C -0.1574 Soluble
222 S C -0.4659 Soluble
223 S C -0.4227 Soluble
224 P C -0.5443 Soluble
225 V C 0.0124 Aggregation-prone
226 T C -0.4213 Soluble
227 K C -0.7228 Soluble
228 S C -0.8430 Soluble
229 F C 0.0000 Buried
230 N C -2.1682 Soluble
231 R C -2.3898 Soluble
232 G C -1.4731 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.656 3.0433 CSV PDB
4.5 -0.6894 3.0257 CSV PDB
5.0 -0.7292 3.0032 CSV PDB
5.5 -0.7674 2.9799 CSV PDB
6.0 -0.7944 2.96 CSV PDB
6.5 -0.8025 2.9477 CSV PDB
7.0 -0.7915 2.9441 CSV PDB
7.5 -0.7679 2.9461 CSV PDB
8.0 -0.7374 2.9508 CSV PDB
8.5 -0.702 2.9571 CSV PDB
9.0 -0.6619 2.9655 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH