Calculation Parameters
| Chain Sequence(s) |
DVQLQESGPSLVKPSQSLSLTCTVTGYSITSDFAWNWIRQFPGNKLEWMGYISYSGNTRYNPSLKSRISITRDTSSNQFFLQLNSVTIEDTATYYCVTAGRGFPYWGQGTLVTVSAAKTTPPSVYPLAPTLGCLVKGYFPESVTVTANSGSLSVHTFPALLQSDLYTMSSSVTVPSSTWSETVTCSVAHPASSTTVDKKADILMTQSPSSMSVSLGDTVSITCHSSQDINSNIGWLQQKPGKSFKGLIYHGTNLDDEVPSRFSGSGSGADYSLTISSLESEDFADYYCVQYAQFPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN
input.pdb
|
| Selected Chain(s) | B (Heavy), A (Light) |
| Distance of Aggregation | 10 Å |
| FoldX Energy Minimization | Yes |
| pH Calculations | Yes |
| Downloads | Download all data (.tar.gz) |
Aggrescan4D Profile
Per-Residue A4D Scores
Download CSVResidues with A4D score > 0.0 are highlighted as aggregation-prone.
| Index | Residue | Chain | A4D Score | Classification |
|---|---|---|---|---|
| 1 | D | B | -2.2308 | Soluble |
| 2 | V | B | 0.0000 | Buried |
| 3 | Q | B | -1.5156 | Soluble |
| 4 | L | B | 0.0000 | Buried |
| 5 | Q | B | -1.2962 | Soluble |
| 6 | E | B | 0.0000 | Buried |
| 7 | S | B | -0.5240 | Soluble |
| 8 | G | B | -0.5319 | Soluble |
| 9 | P | B | -0.2999 | Soluble |
| 11 | S | B | -0.1195 | Soluble |
| 12 | L | B | -0.3133 | Soluble |
| 13 | V | B | 0.0000 | Buried |
| 14 | K | B | -1.4790 | Soluble |
| 15 | P | B | -0.9227 | Soluble |
| 16 | S | B | -0.9732 | Soluble |
| 17 | Q | B | -1.6203 | Soluble |
| 18 | S | B | -1.2594 | Soluble |
| 19 | L | B | 0.0000 | Buried |
| 20 | S | B | -0.7089 | Soluble |
| 21 | L | B | 0.0000 | Buried |
| 22 | T | B | -0.4858 | Soluble |
| 23 | C | B | 0.0000 | Buried |
| 24 | T | B | -0.6265 | Soluble |
| 25 | V | B | 0.0000 | Buried |
| 26 | T | B | -0.8602 | Soluble |
| 27 | G | B | -0.9633 | Soluble |
| 28 | Y | B | -0.5527 | Soluble |
| 29 | S | B | -0.2387 | Soluble |
| 30 | I | B | 0.0000 | Buried |
| 31 | T | B | -0.1506 | Soluble |
| 35 | S | B | -0.3908 | Soluble |
| 36 | D | B | -0.7020 | Soluble |
| 37 | F | B | -0.1495 | Soluble |
| 38 | A | B | -0.2533 | Soluble |
| 39 | W | B | 0.0000 | Buried |
| 40 | N | B | 0.0000 | Buried |
| 41 | W | B | 0.0000 | Buried |
| 42 | I | B | 0.0000 | Buried |
| 43 | R | B | 0.0000 | Buried |
| 44 | Q | B | 0.0000 | Buried |
| 45 | F | B | -1.0535 | Soluble |
| 46 | P | B | -1.0347 | Soluble |
| 47 | G | B | -1.3924 | Soluble |
| 48 | N | B | -1.8738 | Soluble |
| 49 | K | B | -2.2802 | Soluble |
| 50 | L | B | 0.0000 | Buried |
| 51 | E | B | -0.6195 | Soluble |
| 52 | W | B | 0.0000 | Buried |
| 53 | M | B | 0.0000 | Buried |
| 54 | G | B | 0.0000 | Buried |
| 55 | Y | B | -0.3227 | Soluble |
| 56 | I | B | 0.0000 | Buried |
| 57 | S | B | -0.5315 | Soluble |
| 58 | Y | B | 0.0946 | Aggregation-prone |
| 59 | S | B | -0.3964 | Soluble |
| 63 | G | B | -0.8621 | Soluble |
| 64 | N | B | -1.5539 | Soluble |
| 65 | T | B | -1.2162 | Soluble |
| 66 | R | B | -1.6981 | Soluble |
| 67 | Y | B | -0.9561 | Soluble |
| 68 | N | B | 0.0000 | Buried |
| 69 | P | B | -1.1509 | Soluble |
| 70 | S | B | -0.7845 | Soluble |
| 71 | L | B | 0.0000 | Buried |
| 72 | K | B | -2.1447 | Soluble |
| 74 | S | B | -1.3056 | Soluble |
| 75 | R | B | -1.3016 | Soluble |
| 76 | I | B | 0.0000 | Buried |
| 77 | S | B | -0.8873 | Soluble |
| 78 | I | B | 0.0000 | Buried |
| 79 | T | B | -0.6426 | Soluble |
| 80 | R | B | -0.8432 | Soluble |
| 81 | D | B | -1.1051 | Soluble |
| 82 | T | B | -0.7482 | Soluble |
| 83 | S | B | -0.6844 | Soluble |
| 84 | S | B | -0.7234 | Soluble |
| 85 | N | B | -0.7898 | Soluble |
| 86 | Q | B | -0.8324 | Soluble |
| 87 | F | B | 0.0000 | Buried |
| 88 | F | B | -0.2005 | Soluble |
| 89 | L | B | 0.0000 | Buried |
| 90 | Q | B | -0.8795 | Soluble |
| 91 | L | B | 0.0000 | Buried |
| 92 | N | B | -1.1571 | Soluble |
| 93 | S | B | -1.0305 | Soluble |
| 94 | V | B | 0.0000 | Buried |
| 95 | T | B | -0.4177 | Soluble |
| 96 | I | B | 0.4228 | Aggregation-prone |
| 97 | E | B | -1.0904 | Soluble |
| 98 | D | B | 0.0000 | Buried |
| 99 | T | B | -0.0217 | Soluble |
| 100 | A | B | 0.0000 | Buried |
| 101 | T | B | 0.0000 | Buried |
| 102 | Y | B | 0.0000 | Buried |
| 103 | Y | B | 0.0000 | Buried |
| 104 | C | B | 0.0000 | Buried |
| 105 | V | B | 0.0000 | Buried |
| 106 | T | B | 0.0000 | Buried |
| 107 | A | B | -0.2258 | Soluble |
| 108 | G | B | -0.6140 | Soluble |
| 109 | R | B | -0.8612 | Soluble |
| 114 | G | B | 0.0000 | Buried |
| 115 | F | B | 0.0000 | Buried |
| 116 | P | B | 0.0000 | Buried |
| 117 | Y | B | -0.7137 | Soluble |
| 118 | W | B | -0.6776 | Soluble |
| 119 | G | B | 0.0000 | Buried |
| 120 | Q | B | -1.7315 | Soluble |
| 121 | G | B | 0.0000 | Buried |
| 122 | T | B | -0.2680 | Soluble |
| 123 | L | B | 0.3188 | Aggregation-prone |
| 124 | V | B | 0.0000 | Buried |
| 125 | T | B | 0.0000 | Buried |
| 126 | V | B | 0.0000 | Buried |
| 127 | S | B | -0.4230 | Soluble |
| 128 | A | B | -0.5554 | Soluble |
| 129 | A | B | -1.2775 | Soluble |
| 130 | K | B | -1.9943 | Soluble |
| 131 | T | B | -0.9315 | Soluble |
| 132 | T | B | -0.5692 | Soluble |
| 133 | P | B | -0.2832 | Soluble |
| 134 | P | B | 0.0000 | Buried |
| 135 | S | B | -0.5388 | Soluble |
| 136 | V | B | 0.0000 | Buried |
| 137 | Y | B | 0.0000 | Buried |
| 138 | P | B | -0.7220 | Soluble |
| 139 | L | B | 0.0000 | Buried |
| 140 | A | B | -0.0259 | Soluble |
| 141 | P | B | -0.1477 | Soluble |
| 142 | T | B | 0.1317 | Aggregation-prone |
| 143 | L | B | 0.0000 | Buried |
| 144 | G | B | 0.0246 | Aggregation-prone |
| 145 | C | B | -0.2119 | Soluble |
| 146 | L | B | 0.0000 | Buried |
| 147 | V | B | 0.0000 | Buried |
| 148 | K | B | 0.0000 | Buried |
| 149 | G | B | -0.1902 | Soluble |
| 150 | Y | B | 0.0000 | Buried |
| 151 | F | B | 0.0000 | Buried |
| 152 | P | B | 0.0000 | Buried |
| 153 | E | B | -0.3247 | Soluble |
| 154 | S | B | -0.2409 | Soluble |
| 155 | V | B | 0.0913 | Aggregation-prone |
| 156 | T | B | 0.1489 | Aggregation-prone |
| 157 | V | B | 0.1933 | Aggregation-prone |
| 158 | T | B | -0.1000 | Soluble |
| 159 | A | B | -0.1461 | Soluble |
| 160 | N | B | -0.5839 | Soluble |
| 161 | S | B | -0.6156 | Soluble |
| 162 | G | B | -0.6908 | Soluble |
| 163 | S | B | -0.4853 | Soluble |
| 164 | L | B | 0.1502 | Aggregation-prone |
| 165 | S | B | -0.1163 | Soluble |
| 166 | V | B | 0.9023 | Aggregation-prone |
| 167 | H | B | 0.0332 | Aggregation-prone |
| 168 | T | B | -0.0876 | Soluble |
| 169 | F | B | 0.0000 | Buried |
| 170 | P | B | -0.3968 | Soluble |
| 171 | A | B | 0.3105 | Aggregation-prone |
| 172 | L | B | 0.6169 | Aggregation-prone |
| 173 | L | B | 0.8254 | Aggregation-prone |
| 174 | Q | B | -0.0916 | Soluble |
| 175 | S | B | -0.7719 | Soluble |
| 176 | D | B | -1.5721 | Soluble |
| 177 | L | B | -0.4811 | Soluble |
| 178 | Y | B | 0.2262 | Aggregation-prone |
| 179 | T | B | 0.0000 | Buried |
| 180 | M | B | 0.0000 | Buried |
| 181 | S | B | 0.0000 | Buried |
| 182 | S | B | 0.0000 | Buried |
| 183 | S | B | 0.0000 | Buried |
| 184 | V | B | 0.0000 | Buried |
| 185 | T | B | 0.2687 | Aggregation-prone |
| 186 | V | B | 0.0673 | Aggregation-prone |
| 187 | P | B | -0.2406 | Soluble |
| 188 | S | B | -0.5005 | Soluble |
| 189 | S | B | -0.6547 | Soluble |
| 190 | T | B | -0.7968 | Soluble |
| 191 | W | B | -0.1900 | Soluble |
| 192 | S | B | -1.0072 | Soluble |
| 193 | E | B | -1.8254 | Soluble |
| 194 | T | B | -1.0874 | Soluble |
| 195 | V | B | 0.0000 | Buried |
| 196 | T | B | -0.9875 | Soluble |
| 197 | C | B | 0.0000 | Buried |
| 198 | S | B | -0.9766 | Soluble |
| 199 | V | B | 0.0000 | Buried |
| 200 | A | B | -0.1874 | Soluble |
| 201 | H | B | 0.0000 | Buried |
| 202 | P | B | -0.4047 | Soluble |
| 203 | A | B | -0.2921 | Soluble |
| 204 | S | B | -0.2353 | Soluble |
| 205 | S | B | -0.3148 | Soluble |
| 206 | T | B | -0.3872 | Soluble |
| 207 | T | B | -0.5165 | Soluble |
| 208 | V | B | -0.8188 | Soluble |
| 209 | D | B | -2.2048 | Soluble |
| 210 | K | B | -2.0978 | Soluble |
| 211 | K | B | -2.2785 | Soluble |
| 212 | A | B | -0.8536 | Soluble |
| 1 | D | A | -1.6173 | Soluble |
| 2 | I | A | 0.0000 | Buried |
| 3 | L | A | 0.6717 | Aggregation-prone |
| 4 | M | A | 0.0000 | Buried |
| 5 | T | A | -0.3540 | Soluble |
| 6 | Q | A | -0.4151 | Soluble |
| 7 | S | A | -0.5633 | Soluble |
| 8 | P | A | -0.5398 | Soluble |
| 9 | S | A | -0.7061 | Soluble |
| 10 | S | A | -0.6697 | Soluble |
| 11 | M | A | -0.5225 | Soluble |
| 12 | S | A | -0.8022 | Soluble |
| 13 | V | A | -0.5156 | Soluble |
| 14 | S | A | -0.6363 | Soluble |
| 15 | L | A | -0.0813 | Soluble |
| 16 | G | A | -0.7081 | Soluble |
| 17 | D | A | -0.7957 | Soluble |
| 18 | T | A | -0.5350 | Soluble |
| 19 | V | A | 0.0000 | Buried |
| 20 | S | A | -0.3074 | Soluble |
| 21 | I | A | 0.0000 | Buried |
| 22 | T | A | -0.7951 | Soluble |
| 23 | C | A | 0.0000 | Buried |
| 24 | H | A | -1.6739 | Soluble |
| 25 | S | A | 0.0000 | Buried |
| 26 | S | A | -1.2210 | Soluble |
| 27 | Q | A | -2.4916 | Soluble |
| 28 | D | A | -3.0998 | Soluble |
| 29 | I | A | 0.0000 | Buried |
| 36 | N | A | -2.4365 | Soluble |
| 37 | S | A | -1.7414 | Soluble |
| 38 | N | A | -1.3177 | Soluble |
| 39 | I | A | 0.0000 | Buried |
| 40 | G | A | 0.0000 | Buried |
| 41 | W | A | 0.0000 | Buried |
| 42 | L | A | 0.0000 | Buried |
| 43 | Q | A | 0.0000 | Buried |
| 44 | Q | A | 0.0000 | Buried |
| 45 | K | A | -1.7694 | Soluble |
| 46 | P | A | -1.6914 | Soluble |
| 47 | G | A | -1.3997 | Soluble |
| 48 | K | A | -1.7301 | Soluble |
| 49 | S | A | -1.5809 | Soluble |
| 50 | F | A | 0.0000 | Buried |
| 51 | K | A | -1.1554 | Soluble |
| 52 | G | A | 0.0000 | Buried |
| 53 | L | A | 0.0000 | Buried |
| 54 | I | A | 0.0000 | Buried |
| 55 | Y | A | -0.5491 | Soluble |
| 56 | H | A | -1.2469 | Soluble |
| 57 | G | A | 0.0000 | Buried |
| 65 | T | A | -0.6966 | Soluble |
| 66 | N | A | -0.9470 | Soluble |
| 67 | L | A | -0.6683 | Soluble |
| 68 | D | A | 0.0000 | Buried |
| 69 | D | A | -2.8620 | Soluble |
| 70 | E | A | -2.5529 | Soluble |
| 71 | V | A | -1.4739 | Soluble |
| 72 | P | A | -0.8964 | Soluble |
| 74 | S | A | -0.5982 | Soluble |
| 75 | R | A | -0.8078 | Soluble |
| 76 | F | A | 0.0000 | Buried |
| 77 | S | A | -0.4392 | Soluble |
| 78 | G | A | 0.0000 | Buried |
| 79 | S | A | -0.9849 | Soluble |
| 80 | G | A | -1.5710 | Soluble |
| 83 | S | A | -1.8000 | Soluble |
| 84 | G | A | -2.2934 | Soluble |
| 85 | A | A | -2.5764 | Soluble |
| 86 | D | A | -2.8758 | Soluble |
| 87 | Y | A | 0.0000 | Buried |
| 88 | S | A | -0.8711 | Soluble |
| 89 | L | A | 0.0000 | Buried |
| 90 | T | A | -0.3497 | Soluble |
| 91 | I | A | 0.0000 | Buried |
| 92 | S | A | -0.7746 | Soluble |
| 93 | S | A | -0.8985 | Soluble |
| 94 | L | A | 0.0000 | Buried |
| 95 | E | A | -1.9120 | Soluble |
| 96 | S | A | -1.7131 | Soluble |
| 97 | E | A | -2.4561 | Soluble |
| 98 | D | A | 0.0000 | Buried |
| 99 | F | A | 0.0000 | Buried |
| 100 | A | A | 0.0000 | Buried |
| 101 | D | A | -1.0678 | Soluble |
| 102 | Y | A | 0.0000 | Buried |
| 103 | Y | A | 0.0000 | Buried |
| 104 | C | A | 0.0000 | Buried |
| 105 | V | A | 0.0000 | Buried |
| 106 | Q | A | 0.0000 | Buried |
| 107 | Y | A | -0.3063 | Soluble |
| 108 | A | A | -1.0600 | Soluble |
| 109 | Q | A | -0.9621 | Soluble |
| 114 | F | A | -0.4047 | Soluble |
| 115 | P | A | 0.0000 | Buried |
| 116 | W | A | 0.0000 | Buried |
| 117 | T | A | -0.0470 | Soluble |
| 118 | F | A | 0.0113 | Aggregation-prone |
| 119 | G | A | 0.0000 | Buried |
| 120 | G | A | -0.9885 | Soluble |
| 121 | G | A | -1.0311 | Soluble |
| 122 | T | A | 0.0000 | Buried |
| 123 | K | A | -1.0523 | Soluble |
| 124 | L | A | 0.0000 | Buried |
| 125 | E | A | -1.1211 | Soluble |
| 126 | I | A | -0.8914 | Soluble |
| 127 | K | A | -1.8907 | Soluble |
| 128 | R | A | -1.9380 | Soluble |
| 129 | A | A | -1.4030 | Soluble |
| 130 | D | A | -2.4446 | Soluble |
| 131 | A | A | -1.3933 | Soluble |
| 132 | A | A | -0.6700 | Soluble |
| 133 | P | A | 0.0000 | Buried |
| 134 | T | A | -0.1544 | Soluble |
| 135 | V | A | 0.1209 | Aggregation-prone |
| 136 | S | A | 0.0047 | Aggregation-prone |
| 137 | I | A | 0.0653 | Aggregation-prone |
| 138 | F | A | 0.0000 | Buried |
| 139 | P | A | -0.3295 | Soluble |
| 140 | P | A | -0.3883 | Soluble |
| 141 | S | A | 0.0000 | Buried |
| 142 | S | A | -0.8030 | Soluble |
| 143 | E | A | -1.4064 | Soluble |
| 144 | Q | A | 0.0000 | Buried |
| 145 | L | A | -1.0195 | Soluble |
| 146 | T | A | -0.7294 | Soluble |
| 147 | S | A | -0.7636 | Soluble |
| 148 | G | A | -1.1174 | Soluble |
| 149 | G | A | -1.0295 | Soluble |
| 150 | A | A | 0.0000 | Buried |
| 151 | S | A | 0.0000 | Buried |
| 152 | V | A | 0.0000 | Buried |
| 153 | V | A | 0.0000 | Buried |
| 154 | C | A | 0.0000 | Buried |
| 155 | F | A | 0.0000 | Buried |
| 156 | L | A | 0.0000 | Buried |
| 157 | N | A | 0.0000 | Buried |
| 158 | N | A | -1.1636 | Soluble |
| 159 | F | A | 0.0000 | Buried |
| 160 | Y | A | 0.0000 | Buried |
| 161 | P | A | -2.2280 | Soluble |
| 162 | K | A | -2.5909 | Soluble |
| 163 | D | A | -2.8172 | Soluble |
| 164 | I | A | -1.8203 | Soluble |
| 165 | N | A | -2.0175 | Soluble |
| 166 | V | A | -0.7999 | Soluble |
| 167 | K | A | -1.4992 | Soluble |
| 168 | W | A | 0.0000 | Buried |
| 169 | K | A | -2.0934 | Soluble |
| 170 | I | A | 0.0000 | Buried |
| 171 | D | A | -2.4346 | Soluble |
| 172 | G | A | -1.8343 | Soluble |
| 173 | S | A | -1.9909 | Soluble |
| 174 | E | A | -3.0716 | Soluble |
| 175 | R | A | -2.5369 | Soluble |
| 176 | Q | A | -2.5419 | Soluble |
| 177 | N | A | -2.1656 | Soluble |
| 178 | G | A | -1.0246 | Soluble |
| 179 | V | A | -0.6101 | Soluble |
| 180 | L | A | -0.0326 | Soluble |
| 181 | N | A | -0.3527 | Soluble |
| 182 | S | A | 0.0000 | Buried |
| 183 | W | A | -0.4410 | Soluble |
| 184 | T | A | -1.0823 | Soluble |
| 185 | D | A | -2.1428 | Soluble |
| 186 | Q | A | -1.9402 | Soluble |
| 187 | D | A | -2.0144 | Soluble |
| 188 | S | A | -2.1620 | Soluble |
| 189 | K | A | -2.8270 | Soluble |
| 190 | D | A | -2.9255 | Soluble |
| 191 | S | A | -2.2653 | Soluble |
| 192 | T | A | 0.0000 | Buried |
| 193 | Y | A | 0.0000 | Buried |
| 194 | S | A | 0.0000 | Buried |
| 195 | M | A | 0.0000 | Buried |
| 196 | S | A | 0.0000 | Buried |
| 197 | S | A | 0.0000 | Buried |
| 198 | T | A | 0.0000 | Buried |
| 199 | L | A | 0.0000 | Buried |
| 200 | T | A | -0.3005 | Soluble |
| 201 | L | A | 0.0000 | Buried |
| 202 | T | A | -1.4295 | Soluble |
| 203 | K | A | -2.4626 | Soluble |
| 204 | D | A | -3.2813 | Soluble |
| 205 | E | A | -2.5840 | Soluble |
| 206 | Y | A | 0.0000 | Buried |
| 207 | E | A | -3.5201 | Soluble |
| 208 | R | A | -3.6370 | Soluble |
| 209 | H | A | -3.0748 | Soluble |
| 210 | N | A | -3.2327 | Soluble |
| 211 | S | A | -2.1174 | Soluble |
| 212 | Y | A | 0.0000 | Buried |
| 213 | T | A | -1.0249 | Soluble |
| 214 | C | A | 0.0000 | Buried |
| 215 | E | A | -0.4687 | Soluble |
| 216 | A | A | 0.0000 | Buried |
| 217 | T | A | -1.0475 | Soluble |
| 218 | H | A | 0.0000 | Buried |
| 219 | K | A | -2.4495 | Soluble |
| 220 | T | A | -1.3212 | Soluble |
| 221 | S | A | -0.5969 | Soluble |
| 222 | T | A | -0.5107 | Soluble |
| 223 | S | A | -0.3257 | Soluble |
| 224 | P | A | -0.3916 | Soluble |
| 225 | I | A | 0.4626 | Aggregation-prone |
| 226 | V | A | 0.4421 | Aggregation-prone |
| 227 | K | A | -0.4064 | Soluble |
| 228 | S | A | -0.6089 | Soluble |
| 229 | F | A | -1.1973 | Soluble |
| 230 | N | A | -2.5313 | Soluble |
| 231 | R | A | -2.5146 | Soluble |
| 232 | N | A | -2.2403 | Soluble |
3D Structure — colored by A4D score
pH-Dependent Aggregation Profiles
A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.
| pH | Avg A4D Score | Max A4D Score | Downloads |
|---|---|---|---|
| 4.0 | -0.5743 | 2.3354 | CSV PDB |
| 4.5 | -0.6257 | 2.3354 | CSV PDB |
| 5.0 | -0.6849 | 2.3354 | CSV PDB |
| 5.5 | -0.7422 | 2.3354 | CSV PDB |
| 6.0 | -0.788 | 2.3354 | CSV PDB |
| 6.5 | -0.8168 | 2.3354 | CSV PDB |
| 7.0 | -0.8302 | 2.3354 | CSV PDB |
| 7.5 | -0.8333 | 2.3354 | CSV PDB |
| 8.0 | -0.8293 | 2.3354 | CSV PDB |
| 8.5 | -0.8178 | 2.3354 | CSV PDB |
| 9.0 | -0.7966 | 2.3354 | CSV PDB |