Anvatabart / 6my4

A4DAB021_6my4_H_L | Source PDB: 6my4 | Chains: H, L | Done
93.51
Yes
10 Å
Yes
Avg A4D Score
-0.6975
Max Score
1.6664
Min Score
-3.3259
Total Score
-307.61
Score CDR
-0.4966
Score non-CDR
-0.7238

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTWIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGMMFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQDIPRRISGYVAWYQQKPGKAPKLLIYWGSYLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3259
Maximum Score 1.6664
Average Score -0.6975
Total Score -307.6141

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0386 Soluble
2 V H -1.1222 Soluble
3 Q H -1.0785 Soluble
4 L H 0.0000 Buried
5 V H 0.3404 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5525 Soluble
8 G H -0.8956 Soluble
9 G H -0.5006 Soluble
11 G H -0.4558 Soluble
12 L H -0.1312 Soluble
13 V H 0.0000 Buried
14 Q H -1.7333 Soluble
15 P H -1.8727 Soluble
16 G H -1.5551 Soluble
17 G H -1.1898 Soluble
18 S H -1.3198 Soluble
19 L H -1.1503 Soluble
20 R H -2.1147 Soluble
21 L H 0.0000 Buried
22 S H -0.5415 Soluble
23 C H 0.0000 Buried
24 A H -0.3171 Soluble
25 A H 0.0000 Buried
26 S H -1.2329 Soluble
27 G H -1.3847 Soluble
28 F H -1.5988 Soluble
29 N H -2.6315 Soluble
30 I H 0.0000 Buried
35 K H -3.1900 Soluble
36 D H -2.8485 Soluble
37 T H 0.0000 Buried
38 W H -0.1587 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8094 Soluble
45 A H -1.1901 Soluble
46 P H -0.9642 Soluble
47 G H -1.4703 Soluble
48 K H -2.3654 Soluble
49 G H -1.5965 Soluble
50 L H 0.0000 Buried
51 E H -1.0830 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.6969 Soluble
58 P H -1.2627 Soluble
59 T H -1.4399 Soluble
62 N H -1.4755 Soluble
63 G H -0.8021 Soluble
64 Y H -0.2926 Soluble
65 T H -0.4176 Soluble
66 R H -1.1626 Soluble
67 Y H -1.3089 Soluble
68 A H 0.0000 Buried
69 D H -2.4994 Soluble
70 S H -1.7099 Soluble
71 V H 0.0000 Buried
72 K H -2.6563 Soluble
74 G H -1.6973 Soluble
75 R H -1.2591 Soluble
76 F H 0.0000 Buried
77 T H -0.9607 Soluble
78 I H 0.0000 Buried
79 S H -0.3058 Soluble
80 A H -0.9665 Soluble
81 D H -1.6197 Soluble
82 T H -1.8329 Soluble
83 S H -1.6643 Soluble
84 K H -2.3038 Soluble
85 N H -1.9857 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.2923 Soluble
91 M H 0.0000 Buried
92 N H -1.4339 Soluble
93 S H -1.3106 Soluble
94 L H 0.0000 Buried
95 R H -2.5316 Soluble
96 A H -1.8491 Soluble
97 E H -2.2965 Soluble
98 D H 0.0000 Buried
99 T H -0.7789 Soluble
100 A H 0.0000 Buried
101 V H 0.1808 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H 0.0000 Buried
107 W H 0.0000 Buried
108 G H 0.0108 Aggregation-prone
109 G H -0.1954 Soluble
110 M H 0.8683 Aggregation-prone
111 M H 0.8802 Aggregation-prone
112 F H 1.1323 Aggregation-prone
113 Y H 0.8798 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H 0.0108 Aggregation-prone
117 Y H 0.1095 Aggregation-prone
118 W H -0.2831 Soluble
119 G H 0.0000 Buried
120 Q H -1.4821 Soluble
121 G H -0.6929 Soluble
122 T H -0.1076 Soluble
123 L H 0.1669 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4986 Soluble
126 V H 0.0000 Buried
127 S H -0.8491 Soluble
128 S H -0.7374 Soluble
129 A H -0.4566 Soluble
130 S H -0.5526 Soluble
131 T H -0.7141 Soluble
132 K H -1.1382 Soluble
133 G H -1.3747 Soluble
134 P H 0.0000 Buried
135 S H -0.2468 Soluble
136 V H 0.0000 Buried
137 F H -0.6653 Soluble
138 P H -1.2028 Soluble
139 L H 0.0000 Buried
140 A H -1.1496 Soluble
141 P H 0.0000 Buried
142 S H -1.2752 Soluble
143 S H -1.2217 Soluble
144 K H -1.7806 Soluble
145 S H -1.0971 Soluble
146 T H -0.9114 Soluble
147 S H -0.7162 Soluble
148 G H -0.7936 Soluble
149 G H -0.7981 Soluble
150 T H -0.5952 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.2589 Soluble
159 D H -0.4581 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.5147 Soluble
164 P H -0.7649 Soluble
165 V H -0.7275 Soluble
166 T H -0.6246 Soluble
167 V H -0.3773 Soluble
168 S H -0.4298 Soluble
169 W H 0.0000 Buried
170 N H -0.8285 Soluble
171 S H -0.7040 Soluble
172 G H -0.5493 Soluble
173 A H -0.2725 Soluble
174 L H -0.0223 Soluble
175 T H -0.1864 Soluble
176 S H -0.1807 Soluble
177 G H -0.2157 Soluble
178 V H 0.1774 Aggregation-prone
179 H H -0.3291 Soluble
180 T H 0.0144 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3209 Soluble
183 A H 0.2120 Aggregation-prone
184 V H 0.5572 Aggregation-prone
185 L H 1.1273 Aggregation-prone
186 Q H 0.2496 Aggregation-prone
187 S H -0.1713 Soluble
188 S H -0.2271 Soluble
189 G H 0.0139 Aggregation-prone
190 L H 0.1213 Aggregation-prone
191 Y H 0.4200 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.2332 Soluble
199 V H 0.0000 Buried
200 P H -0.5935 Soluble
201 S H -0.7320 Soluble
202 S H -0.5831 Soluble
203 S H -0.7223 Soluble
204 L H -0.9844 Soluble
205 G H -1.5285 Soluble
206 T H -1.1162 Soluble
207 Q H -1.5847 Soluble
208 T H -1.3138 Soluble
209 Y H 0.0000 Buried
210 I H -1.3731 Soluble
211 C H 0.0000 Buried
212 N H -1.5110 Soluble
213 V H 0.0000 Buried
214 N H -2.0961 Soluble
215 H H 0.0000 Buried
216 K H -2.8163 Soluble
217 P H -1.6237 Soluble
218 S H -1.8339 Soluble
219 N H -2.6261 Soluble
220 T H -2.1142 Soluble
221 K H -2.7463 Soluble
222 V H -1.6259 Soluble
223 D H -2.5785 Soluble
224 K H -2.2329 Soluble
225 K H -2.5492 Soluble
226 V H 0.0000 Buried
227 E H -2.9860 Soluble
228 P H -1.8970 Soluble
229 K H -2.2895 Soluble
230 S H -0.9107 Soluble
231 C H 0.1165 Aggregation-prone
1 D L -2.1056 Soluble
2 I L -1.6229 Soluble
3 Q L -1.8492 Soluble
4 M L 0.0000 Buried
5 T L -1.2459 Soluble
6 Q L 0.0000 Buried
7 S L -0.7304 Soluble
8 P L -0.5070 Soluble
9 S L -0.8499 Soluble
10 S L -0.7504 Soluble
11 L L -0.4851 Soluble
12 S L -0.7278 Soluble
13 A L -0.9280 Soluble
14 S L -0.8876 Soluble
15 V L -0.1926 Soluble
16 G L -0.9288 Soluble
17 D L -1.9490 Soluble
18 R L -2.3754 Soluble
19 V L 0.0000 Buried
20 T L -0.6565 Soluble
21 I L 0.0000 Buried
22 T L -0.6868 Soluble
23 C L 0.0000 Buried
24 R L -2.3802 Soluble
25 A L 0.0000 Buried
26 S L -2.0919 Soluble
27 Q L -2.7701 Soluble
28 D L -2.7583 Soluble
29 I L 0.0000 Buried
30 P L -1.8490 Soluble
31 R L -2.8316 Soluble
34 R L -2.2526 Soluble
35 I L -0.2739 Soluble
36 S L -0.1881 Soluble
37 G L 0.0000 Buried
38 Y L 0.8896 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6591 Soluble
46 P L -1.4450 Soluble
47 G L -1.5853 Soluble
48 K L -2.5421 Soluble
49 A L -1.5474 Soluble
50 P L 0.0000 Buried
51 K L -1.2876 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.1292 Aggregation-prone
56 W L 1.1057 Aggregation-prone
57 G L 0.4603 Aggregation-prone
65 S L 0.6507 Aggregation-prone
66 Y L 1.6664 Aggregation-prone
67 L L 1.2676 Aggregation-prone
68 Y L 0.4834 Aggregation-prone
69 S L -0.0303 Soluble
70 G L -0.4658 Soluble
71 V L 0.0000 Buried
72 P L -0.3630 Soluble
74 S L -0.3881 Soluble
75 R L -0.7999 Soluble
76 F L 0.0000 Buried
77 S L -0.0408 Soluble
78 G L 0.0734 Aggregation-prone
79 S L -0.3828 Soluble
80 G L -0.9722 Soluble
83 S L -0.7799 Soluble
84 G L -1.5170 Soluble
85 T L -1.8524 Soluble
86 D L -1.5303 Soluble
87 F L -0.9235 Soluble
88 T L -0.5862 Soluble
89 L L 0.0000 Buried
90 T L -0.5860 Soluble
91 I L 0.0000 Buried
92 S L -1.3857 Soluble
93 S L -1.1972 Soluble
94 L L 0.0000 Buried
95 Q L -0.9320 Soluble
96 P L -1.6441 Soluble
97 E L -1.9874 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.8452 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.0000 Buried
108 Y L 0.4038 Aggregation-prone
109 T L -0.1869 Soluble
114 T L -0.2104 Soluble
115 P L -0.4611 Soluble
116 P L 0.0000 Buried
117 T L -0.5537 Soluble
118 F L -0.4244 Soluble
119 G L 0.0000 Buried
120 Q L -1.6765 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.4535 Soluble
124 V L 0.0000 Buried
125 E L -1.0787 Soluble
126 I L 0.0000 Buried
127 K L -1.2577 Soluble
128 R L -0.8770 Soluble
129 T L -0.0734 Soluble
130 V L 0.4320 Aggregation-prone
131 A L 0.0120 Aggregation-prone
132 A L -0.1431 Soluble
133 P L 0.0000 Buried
134 S L -0.3589 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L -0.0047 Soluble
138 F L 0.0000 Buried
139 P L -0.4147 Soluble
140 P L -0.7982 Soluble
141 S L -1.8195 Soluble
142 D L -3.0906 Soluble
143 E L -2.8213 Soluble
144 Q L 0.0000 Buried
145 L L 0.0000 Buried
146 K L -2.8885 Soluble
147 S L -1.7761 Soluble
148 G L -1.3096 Soluble
149 T L -0.9489 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9992 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6419 Soluble
162 R L -2.8377 Soluble
163 E L -3.1311 Soluble
164 A L -2.3041 Soluble
165 K L -2.4249 Soluble
166 V L -1.0836 Soluble
167 Q L -0.5745 Soluble
168 W L 0.0000 Buried
169 K L -0.6266 Soluble
170 V L 0.0000 Buried
171 D L -1.7551 Soluble
172 N L -1.4972 Soluble
173 A L -0.2769 Soluble
174 L L 0.6602 Aggregation-prone
175 Q L -0.1185 Soluble
176 S L -0.3589 Soluble
177 G L -0.7543 Soluble
178 N L -0.8780 Soluble
179 S L -1.0721 Soluble
180 Q L -1.2781 Soluble
181 E L -1.6448 Soluble
182 S L 0.0000 Buried
183 V L -0.7703 Soluble
184 T L -1.1794 Soluble
185 E L -2.2195 Soluble
186 Q L 0.0000 Buried
187 D L -2.1530 Soluble
188 S L -2.3113 Soluble
189 K L -2.6888 Soluble
190 D L -2.0965 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6024 Soluble
199 L L 0.0000 Buried
200 T L -0.4728 Soluble
201 L L -0.6397 Soluble
202 S L -1.2502 Soluble
203 K L -2.1353 Soluble
204 A L -1.8462 Soluble
205 D L -2.4103 Soluble
206 Y L 0.0000 Buried
207 E L -3.0629 Soluble
208 K L -3.3259 Soluble
209 H L -2.5653 Soluble
210 K L -2.1737 Soluble
211 V L -0.6535 Soluble
212 Y L 0.0000 Buried
213 A L -0.5722 Soluble
214 C L 0.0000 Buried
215 E L -0.8842 Soluble
216 V L 0.0000 Buried
217 T L -1.2560 Soluble
218 H L 0.0000 Buried
219 Q L -1.7005 Soluble
220 G L -0.3981 Soluble
221 L L -0.2256 Soluble
222 S L -0.4727 Soluble
223 S L -0.4474 Soluble
224 P L -0.6083 Soluble
225 V L -0.0897 Soluble
226 T L -0.4538 Soluble
227 K L -0.6078 Soluble
228 S L -0.4263 Soluble
229 F L -0.5039 Soluble
230 N L -1.3185 Soluble
231 R L -1.8367 Soluble
232 G L -1.6745 Soluble
233 E L -1.8972 Soluble
234 C L -0.6635 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7266 2.158 CSV PDB
4.5 -0.7689 2.1527 CSV PDB
5.0 -0.8173 2.1472 CSV PDB
5.5 -0.8621 2.1417 CSV PDB
6.0 -0.8921 2.1362 CSV PDB
6.5 -0.8985 2.1307 CSV PDB
7.0 -0.882 2.1255 CSV PDB
7.5 -0.8509 2.1207 CSV PDB
8.0 -0.8118 2.1171 CSV PDB
8.5 -0.7665 2.1148 CSV PDB
9.0 -0.7146 2.1133 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH