Tarcocimab / 1bj1

A4DAB257_1bj1_K_J | Source PDB: 1bj1 | Chains: K, J | Done
97.83
Yes
10 Å
Yes
Avg A4D Score
-0.6424
Max Score
1.4163
Min Score
-3.1731
Total Score
-276.86
Score CDR
-0.171
Score non-CDR
-0.7042

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGYTFTNYGMNWVRQAPGKGLEWVGWINTYTGEPTYAADFKRRFTFSLDTSKSTAYLQMNSLRAEDTAVYYCAKYPHYYGSSHWYFDVWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRVTITCSASQDISNYLNWYQQKPGKAPKVLIYFTSSLHSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYSTVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) K (Heavy), J (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.1731
Maximum Score 1.4163
Average Score -0.6424
Total Score -276.8638

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E K -2.1077 Soluble
2 V K -1.2412 Soluble
3 Q K -1.2477 Soluble
4 L K 0.0000 Buried
5 V K 1.1245 Aggregation-prone
6 E K 0.0000 Buried
7 S K -0.4022 Soluble
8 G K -0.8592 Soluble
9 G K -0.5027 Soluble
11 G K -0.3653 Soluble
12 L K -0.1177 Soluble
13 V K 0.0000 Buried
14 Q K -1.6951 Soluble
15 P K -1.7914 Soluble
16 G K -1.4529 Soluble
17 G K -1.2123 Soluble
18 S K -1.1824 Soluble
19 L K -1.1751 Soluble
20 R K -2.0799 Soluble
21 L K 0.0000 Buried
22 S K -0.3508 Soluble
23 C K 0.0000 Buried
24 A K 0.0000 Buried
25 A K 0.0000 Buried
26 S K -0.9003 Soluble
27 G K -1.2547 Soluble
28 Y K -0.6584 Soluble
29 T K -0.4337 Soluble
30 F K 0.0000 Buried
35 T K -0.2255 Soluble
36 N K -1.0643 Soluble
37 Y K -0.4610 Soluble
38 G K -0.2865 Soluble
39 M K 0.0000 Buried
40 N K 0.0000 Buried
41 W K 0.0000 Buried
42 V K 0.0000 Buried
43 R K -0.6437 Soluble
44 Q K -0.9454 Soluble
45 A K -1.2521 Soluble
46 P K -0.9794 Soluble
47 G K -1.4799 Soluble
48 K K -2.4392 Soluble
49 G K -1.7462 Soluble
50 L K 0.0000 Buried
51 E K -1.3284 Soluble
52 W K 0.0000 Buried
53 V K 0.0000 Buried
54 G K 0.0000 Buried
55 W K -0.0789 Soluble
56 I K 0.0000 Buried
57 N K -0.6495 Soluble
58 T K 0.0000 Buried
59 Y K 0.5895 Aggregation-prone
62 T K -0.1459 Soluble
63 G K -0.8220 Soluble
64 E K -1.7950 Soluble
65 P K -0.9755 Soluble
66 T K -0.3846 Soluble
67 Y K -0.6184 Soluble
68 A K 0.0000 Buried
69 A K -1.5995 Soluble
70 D K -2.5991 Soluble
71 F K 0.0000 Buried
72 K K -3.0860 Soluble
74 R K -3.1731 Soluble
75 R K -2.0867 Soluble
76 F K 0.0000 Buried
77 T K -1.0799 Soluble
78 F K 0.0000 Buried
79 S K -0.4515 Soluble
80 L K -0.5476 Soluble
81 D K -1.0997 Soluble
82 T K -0.8262 Soluble
83 S K -1.2269 Soluble
84 K K -2.0042 Soluble
85 S K -1.0915 Soluble
86 T K 0.0000 Buried
87 A K 0.0000 Buried
88 Y K 0.0000 Buried
89 L K 0.0000 Buried
90 Q K -1.2315 Soluble
91 M K 0.0000 Buried
92 N K -1.5507 Soluble
93 S K -1.3644 Soluble
94 L K 0.0000 Buried
95 R K -2.2218 Soluble
96 A K -1.6955 Soluble
97 E K -2.1910 Soluble
98 D K 0.0000 Buried
99 T K -0.7045 Soluble
100 A K 0.0000 Buried
101 V K 0.2013 Aggregation-prone
102 Y K 0.0000 Buried
103 Y K 0.0000 Buried
104 C K 0.0000 Buried
105 A K 0.0000 Buried
106 K K 0.0000 Buried
107 Y K 0.0000 Buried
108 P K 0.0000 Buried
109 H K -0.3243 Soluble
110 Y K 1.2941 Aggregation-prone
111 Y K 1.4163 Aggregation-prone
111A G K 0.3756 Aggregation-prone
112B S K 0.1140 Aggregation-prone
112A S K 0.3195 Aggregation-prone
112 H K 0.5966 Aggregation-prone
113 W K 0.6336 Aggregation-prone
114 Y K 0.0000 Buried
115 F K 0.0000 Buried
116 D K -0.6420 Soluble
117 V K -0.3123 Soluble
118 W K -0.3756 Soluble
119 G K 0.0000 Buried
120 Q K -1.2077 Soluble
121 G K -0.5247 Soluble
122 T K -0.0436 Soluble
123 L K 0.1680 Aggregation-prone
124 V K 0.0000 Buried
125 T K 0.0000 Buried
126 V K 0.0000 Buried
127 S K -0.7888 Soluble
128 S K -0.7633 Soluble
129 A K -0.5069 Soluble
130 S K -0.6637 Soluble
131 T K -0.8450 Soluble
132 K K -1.8096 Soluble
133 G K -1.7009 Soluble
134 P K 0.0000 Buried
135 S K -0.6319 Soluble
136 V K 0.0000 Buried
137 F K 0.0000 Buried
138 P K -1.0698 Soluble
139 L K 0.0000 Buried
140 A K -1.1619 Soluble
141 P K 0.0000 Buried
142 S K -0.6098 Soluble
143 G K -0.6773 Soluble
144 T K -0.5149 Soluble
145 A K -0.2913 Soluble
146 A K 0.0000 Buried
147 L K 0.0000 Buried
148 G K 0.0000 Buried
149 C K 0.0000 Buried
150 L K 0.0000 Buried
151 V K 0.0000 Buried
152 K K 0.0000 Buried
153 D K -0.9917 Soluble
154 Y K 0.0000 Buried
155 F K -0.5031 Soluble
156 P K 0.0000 Buried
157 E K -0.4704 Soluble
158 P K -0.7329 Soluble
159 V K -0.8039 Soluble
160 T K -0.5924 Soluble
161 V K -0.2358 Soluble
162 S K -0.3243 Soluble
163 W K 0.0000 Buried
164 N K -0.6490 Soluble
165 S K -0.6069 Soluble
166 G K -0.5255 Soluble
167 A K -0.2021 Soluble
168 L K -0.0207 Soluble
169 T K -0.1824 Soluble
170 S K -0.1633 Soluble
171 G K -0.1944 Soluble
172 V K 0.1559 Aggregation-prone
173 H K -0.3293 Soluble
174 T K -0.0231 Soluble
175 F K 0.0000 Buried
176 P K -0.0665 Soluble
177 A K 0.5279 Aggregation-prone
178 V K 1.0355 Aggregation-prone
179 L K 1.3995 Aggregation-prone
180 Q K 0.3742 Aggregation-prone
181 S K -0.1185 Soluble
182 S K -0.3110 Soluble
183 G K -0.0643 Soluble
184 L K 0.0020 Aggregation-prone
185 Y K 0.5356 Aggregation-prone
186 S K 0.0000 Buried
187 L K 0.3707 Aggregation-prone
188 S K 0.0000 Buried
189 S K 0.0000 Buried
190 V K 0.0000 Buried
191 V K 0.0000 Buried
192 T K -0.1948 Soluble
193 V K 0.0000 Buried
194 P K -0.5698 Soluble
195 S K -0.6459 Soluble
196 S K -0.6540 Soluble
197 S K -0.6447 Soluble
198 L K -0.8479 Soluble
199 G K -0.9792 Soluble
200 T K -0.6859 Soluble
201 Q K -1.1361 Soluble
202 T K -1.0072 Soluble
203 Y K 0.0000 Buried
204 I K -1.0908 Soluble
205 C K 0.0000 Buried
206 N K -1.3309 Soluble
207 V K 0.0000 Buried
208 N K -1.7289 Soluble
209 H K 0.0000 Buried
210 K K -2.7623 Soluble
211 P K -1.6738 Soluble
212 S K -1.9177 Soluble
213 N K -2.6174 Soluble
214 T K -2.2358 Soluble
215 K K -2.5792 Soluble
216 V K -1.5436 Soluble
217 D K -2.1547 Soluble
218 K K -1.9557 Soluble
219 K K -2.1273 Soluble
220 V K 0.0000 Buried
221 E K -2.5777 Soluble
222 P K -1.7963 Soluble
223 K K -2.1783 Soluble
1 D J -1.8789 Soluble
2 I J 0.0000 Buried
3 Q J -2.0331 Soluble
4 M J 0.0000 Buried
5 T J -0.9355 Soluble
6 Q J 0.0000 Buried
7 S J -0.6648 Soluble
8 P J -0.6911 Soluble
9 S J -0.9779 Soluble
10 S J -0.9980 Soluble
11 L J -0.7505 Soluble
12 S J -1.1500 Soluble
13 A J 0.0000 Buried
14 S J -1.0299 Soluble
15 V J -0.0991 Soluble
16 G J -0.7745 Soluble
17 D J -1.7496 Soluble
18 R J -2.2314 Soluble
19 V J 0.0000 Buried
20 T J -0.5950 Soluble
21 I J 0.0000 Buried
22 T J -0.5408 Soluble
23 C J 0.0000 Buried
24 S J -1.4860 Soluble
25 A J 0.0000 Buried
26 S J -1.9727 Soluble
27 Q J -2.6556 Soluble
28 D J -2.7161 Soluble
29 I J 0.0000 Buried
36 S J -1.0523 Soluble
37 N J -0.4882 Soluble
38 Y J 0.4118 Aggregation-prone
39 L J 0.0000 Buried
40 N J 0.0000 Buried
41 W J 0.0000 Buried
42 Y J 0.0000 Buried
43 Q J -0.9829 Soluble
44 Q J -1.3314 Soluble
45 K J -1.7347 Soluble
46 P J -1.4301 Soluble
47 G J -1.5276 Soluble
48 K J -2.2580 Soluble
49 A J -1.4001 Soluble
50 P J 0.0000 Buried
51 K J -1.4898 Soluble
52 V J 0.0000 Buried
53 L J 0.0000 Buried
54 I J 0.0000 Buried
55 Y J 0.7359 Aggregation-prone
56 F J 0.9619 Aggregation-prone
57 T J 0.0000 Buried
65 S J 0.0522 Aggregation-prone
66 S J 0.3433 Aggregation-prone
67 L J 0.4418 Aggregation-prone
68 H J -0.1406 Soluble
69 S J -0.3970 Soluble
70 G J -0.5636 Soluble
71 V J 0.0000 Buried
72 P J -0.3893 Soluble
74 S J -0.4186 Soluble
75 R J -0.7303 Soluble
76 F J 0.0000 Buried
77 S J -0.3191 Soluble
78 G J -0.1641 Soluble
79 S J -0.6939 Soluble
80 G J -1.0439 Soluble
83 S J -1.2428 Soluble
84 G J -1.7925 Soluble
85 T J -2.0820 Soluble
86 D J -2.4651 Soluble
87 F J 0.0000 Buried
88 T J -0.6739 Soluble
89 L J 0.0000 Buried
90 T J -0.6149 Soluble
91 I J 0.0000 Buried
92 S J -1.3278 Soluble
93 S J -1.1326 Soluble
94 L J 0.0000 Buried
95 Q J -0.8661 Soluble
96 P J -1.4191 Soluble
97 E J -1.5008 Soluble
98 D J 0.0000 Buried
99 F J 0.0000 Buried
100 A J 0.0000 Buried
101 T J 0.0000 Buried
102 Y J 0.0000 Buried
103 Y J 0.0000 Buried
104 C J 0.0000 Buried
105 Q J 0.0000 Buried
106 Q J 0.0000 Buried
107 Y J 0.0000 Buried
108 S J 0.0254 Aggregation-prone
109 T J 0.5004 Aggregation-prone
114 V J 1.3762 Aggregation-prone
115 P J 0.3394 Aggregation-prone
116 W J 0.0000 Buried
117 T J -0.5927 Soluble
118 F J -0.5637 Soluble
119 G J 0.0000 Buried
120 Q J -1.7790 Soluble
121 G J 0.0000 Buried
122 T J 0.0000 Buried
123 K J -1.9541 Soluble
124 V J 0.0000 Buried
125 E J -1.4420 Soluble
126 I J 0.0000 Buried
127 K J -1.9550 Soluble
128 R J -1.0534 Soluble
129 T J -0.1332 Soluble
130 V J 0.3999 Aggregation-prone
131 A J 0.0458 Aggregation-prone
132 A J -0.1101 Soluble
133 P J -0.0987 Soluble
134 S J -0.0898 Soluble
135 V J 0.0000 Buried
136 F J 0.0544 Aggregation-prone
137 I J 0.0423 Aggregation-prone
138 F J 0.0000 Buried
139 P J -0.6726 Soluble
140 P J -0.8000 Soluble
141 S J -1.7876 Soluble
142 D J -2.9249 Soluble
143 E J -3.1250 Soluble
144 Q J 0.0000 Buried
145 L J -2.3186 Soluble
146 K J -2.9179 Soluble
147 S J -1.8936 Soluble
148 G J -1.6119 Soluble
149 T J -1.4718 Soluble
150 A J 0.0000 Buried
151 S J 0.0000 Buried
152 V J 0.0000 Buried
153 V J 0.0000 Buried
154 C J 0.0000 Buried
155 L J 0.0000 Buried
156 L J 0.0000 Buried
157 N J 0.0000 Buried
158 N J -0.8687 Soluble
159 F J 0.0000 Buried
160 Y J -1.0084 Soluble
161 P J -1.6079 Soluble
162 R J -2.5801 Soluble
163 E J -3.0523 Soluble
164 A J -2.2278 Soluble
165 K J -2.2368 Soluble
166 V J -0.9096 Soluble
167 Q J -0.6026 Soluble
168 W J 0.0000 Buried
169 K J -0.3454 Soluble
170 V J 0.0000 Buried
171 D J -1.2173 Soluble
172 N J -1.1747 Soluble
173 A J -0.1474 Soluble
174 L J 0.6595 Aggregation-prone
175 Q J -0.2917 Soluble
176 S J -0.5092 Soluble
177 G J -0.9491 Soluble
178 N J -0.9513 Soluble
179 S J -0.8761 Soluble
180 Q J -0.7278 Soluble
181 E J -0.8000 Soluble
182 S J -0.2495 Soluble
183 V J -0.0623 Soluble
184 T J -1.0288 Soluble
185 E J -2.1677 Soluble
186 Q J 0.0000 Buried
187 D J -2.1437 Soluble
188 S J -2.0754 Soluble
189 K J -2.4368 Soluble
190 D J -1.6893 Soluble
191 S J 0.0000 Buried
192 T J 0.0000 Buried
193 Y J 0.0000 Buried
194 S J 0.0000 Buried
195 L J 0.0000 Buried
196 S J 0.0000 Buried
197 S J 0.0000 Buried
198 T J -0.3777 Soluble
199 L J 0.0000 Buried
200 T J -0.3030 Soluble
201 L J -0.7265 Soluble
202 S J -1.2437 Soluble
203 K J -2.3453 Soluble
204 A J -1.9276 Soluble
205 D J -2.8276 Soluble
206 Y J 0.0000 Buried
207 E J -2.6295 Soluble
208 K J -3.0804 Soluble
209 H J -2.2321 Soluble
210 K J -1.9821 Soluble
211 V J -0.3084 Soluble
212 Y J 0.0000 Buried
213 A J 0.0000 Buried
214 C J 0.0000 Buried
215 E J -0.6711 Soluble
216 V J 0.0000 Buried
217 T J -1.1754 Soluble
218 H J 0.0000 Buried
219 Q J -1.7115 Soluble
220 G J -0.5731 Soluble
221 L J -0.2545 Soluble
222 S J -0.4801 Soluble
223 S J -0.3594 Soluble
224 P J -0.4357 Soluble
225 V J 0.2956 Aggregation-prone
226 T J -0.3424 Soluble
227 K J -0.7091 Soluble
228 S J -0.6138 Soluble
229 F J -0.8458 Soluble
230 N J -1.7767 Soluble
231 R J -2.1158 Soluble
232 G J -1.9410 Soluble
233 E J -2.5616 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6688 1.9979 CSV PDB
4.5 -0.7129 1.9583 CSV PDB
5.0 -0.7633 1.942 CSV PDB
5.5 -0.8099 1.9232 CSV PDB
6.0 -0.8417 1.9048 CSV PDB
6.5 -0.8505 1.8898 CSV PDB
7.0 -0.8368 1.8803 CSV PDB
7.5 -0.8086 1.876 CSV PDB
8.0 -0.7724 1.8743 CSV PDB
8.5 -0.7297 1.8738 CSV PDB
9.0 -0.68 1.8736 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH