Nirsevimab / 6s3d

A4DAB181_6s3d_H_L | Source PDB: 6s3d | Chains: H, L | Done
96.14
Yes
10 Å
Yes
Avg A4D Score
-0.5805
Max Score
2.6879
Min Score
-4.2514
Total Score
-245.55
Score CDR
-0.0273
Score non-CDR
-0.6597

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGSSVMVSCQASGGPLRNYIINWLRQAPGQGPEWMGGIIPVLGTVHYAPKFQGRVTITADESTDTAYIHLISLRSEDTAMYYCATETALVVSTTYLPHYFDNWGQGTLVTVSSASTKGPSVFPLATAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKDIQMTQSPSSLSAAVGDRVTITCQASQDIVNYLNWYQQKPGKAPKLLIYVASNLETGVPSRFSGSGSGTDFSLTISSLQPEDVATYYCQQYDNLPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.2514
Maximum Score 2.6879
Average Score -0.5805
Total Score -245.5472

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.8755 Aggregation-prone
3 Q H -0.4581 Soluble
4 L H 0.0000 Buried
5 V H 0.4967 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.1917 Soluble
8 G H -1.0931 Soluble
9 A H -1.0841 Soluble
11 E H -1.5358 Soluble
12 V H -0.9226 Soluble
13 K H -1.5699 Soluble
14 K H -1.9063 Soluble
15 P H -1.7162 Soluble
16 G H -1.3474 Soluble
17 S H -0.9478 Soluble
18 S H -0.3356 Soluble
19 V H 0.0000 Buried
20 M H 0.8594 Aggregation-prone
21 V H 0.0000 Buried
22 S H 0.2496 Aggregation-prone
23 C H 0.0000 Buried
24 Q H -0.4150 Soluble
25 A H 0.0000 Buried
26 S H -0.3327 Soluble
27 G H -0.3727 Soluble
28 G H -0.4733 Soluble
29 P H -1.1972 Soluble
30 L H 0.0000 Buried
35 R H -1.5510 Soluble
36 N H -0.2339 Soluble
37 Y H -0.1043 Soluble
38 I H 0.0000 Buried
39 I H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 L H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.6178 Soluble
45 A H -0.9926 Soluble
46 P H -1.0484 Soluble
47 G H -1.2121 Soluble
48 Q H -1.7295 Soluble
49 G H -1.0442 Soluble
50 P H 0.0000 Buried
51 E H -0.5415 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 I H 0.0000 Buried
58 P H -0.5695 Soluble
59 V H 0.2401 Aggregation-prone
62 L H 0.4384 Aggregation-prone
63 G H -0.1116 Soluble
64 T H 0.3694 Aggregation-prone
65 V H 0.6932 Aggregation-prone
66 H H 0.2496 Aggregation-prone
67 Y H -0.3848 Soluble
68 A H 0.0000 Buried
69 P H -1.6833 Soluble
70 K H -2.2343 Soluble
71 F H 0.0000 Buried
72 Q H -2.0502 Soluble
74 G H -1.3909 Soluble
75 R H -0.9895 Soluble
76 V H 0.0000 Buried
77 T H -0.1374 Soluble
78 I H 0.0000 Buried
79 T H -0.2127 Soluble
80 A H -0.8936 Soluble
81 D H -1.6630 Soluble
82 E H -2.2672 Soluble
83 S H -1.2316 Soluble
84 T H -1.0200 Soluble
85 D H -1.6031 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H 0.1985 Aggregation-prone
89 I H 0.0000 Buried
90 H H 0.1669 Aggregation-prone
91 L H 0.0000 Buried
92 I H 0.1026 Aggregation-prone
93 S H -0.7387 Soluble
94 L H 0.0000 Buried
95 R H -2.7599 Soluble
96 S H -2.2162 Soluble
97 E H -2.4964 Soluble
98 D H 0.0000 Buried
99 T H -0.9902 Soluble
100 A H 0.0000 Buried
101 M H 0.0205 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 T H 0.0000 Buried
107 E H 0.0000 Buried
108 T H 0.3518 Aggregation-prone
109 A H 0.0000 Buried
110 L H 2.2704 Aggregation-prone
111 V H 2.6879 Aggregation-prone
111A V H 1.9082 Aggregation-prone
111B S H 0.8893 Aggregation-prone
111C T H 0.2465 Aggregation-prone
112C T H 0.2534 Aggregation-prone
112B Y H 0.7958 Aggregation-prone
112A L H 0.5862 Aggregation-prone
112 P H 0.0000 Buried
113 H H 0.1367 Aggregation-prone
114 Y H 0.2545 Aggregation-prone
115 F H 0.0000 Buried
116 D H -1.0558 Soluble
117 N H -0.7193 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.4462 Soluble
121 G H 0.0000 Buried
122 T H 0.0000 Buried
123 L H -0.3053 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.0424 Soluble
128 S H -0.7733 Soluble
129 A H -0.6103 Soluble
130 S H -0.8009 Soluble
131 T H -0.9306 Soluble
132 K H -1.4878 Soluble
133 G H -1.4897 Soluble
134 P H -0.8887 Soluble
135 S H -0.5556 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9248 Soluble
139 L H 0.0000 Buried
140 A H 0.1256 Aggregation-prone
141 T H -0.1536 Soluble
142 A H 0.1230 Aggregation-prone
143 A H 0.1488 Aggregation-prone
144 L H 0.3474 Aggregation-prone
145 G H 0.0000 Buried
146 C H 0.0000 Buried
147 L H 0.0000 Buried
148 V H 0.0000 Buried
149 K H -0.3814 Soluble
150 D H -0.7471 Soluble
151 Y H 0.0000 Buried
152 F H 0.0000 Buried
153 P H 0.0000 Buried
154 E H -0.6407 Soluble
155 P H -0.8135 Soluble
156 V H 0.0000 Buried
157 T H -0.3815 Soluble
158 V H -0.2029 Soluble
159 S H -0.3252 Soluble
160 W H 0.0000 Buried
161 N H -0.3477 Soluble
162 S H -0.5913 Soluble
163 G H -0.5515 Soluble
164 A H -0.2160 Soluble
165 L H 0.1118 Aggregation-prone
166 T H -0.1013 Soluble
167 S H -0.1509 Soluble
168 G H -0.1402 Soluble
169 V H 0.2336 Aggregation-prone
170 H H -0.1700 Soluble
171 T H 0.0309 Aggregation-prone
172 F H 0.0000 Buried
173 P H -0.4019 Soluble
174 A H 0.2292 Aggregation-prone
175 V H 0.5343 Aggregation-prone
176 L H 1.3568 Aggregation-prone
177 Q H 0.1640 Aggregation-prone
178 S H -0.1745 Soluble
179 S H -0.2447 Soluble
180 G H -0.2568 Soluble
181 L H -0.0367 Soluble
182 Y H 0.3631 Aggregation-prone
183 S H 0.2472 Aggregation-prone
184 L H 0.0000 Buried
185 S H 0.0000 Buried
186 S H 0.0000 Buried
187 V H 0.0000 Buried
188 V H 0.0000 Buried
189 T H -0.0792 Soluble
190 V H 0.0000 Buried
191 P H -0.1879 Soluble
192 S H -0.1154 Soluble
193 S H -0.1793 Soluble
194 S H 0.0534 Aggregation-prone
195 L H 0.9110 Aggregation-prone
196 G H -0.0662 Soluble
197 T H -0.3157 Soluble
198 Q H -0.4779 Soluble
199 T H -0.0226 Soluble
200 Y H 0.3178 Aggregation-prone
201 I H -0.1999 Soluble
202 C H 0.0000 Buried
203 N H -1.4008 Soluble
204 V H 0.0000 Buried
205 N H -1.7242 Soluble
206 H H 0.0000 Buried
207 K H -2.9621 Soluble
208 P H -1.9607 Soluble
209 S H -1.9809 Soluble
210 N H -2.5360 Soluble
211 T H -2.0688 Soluble
212 K H -2.6363 Soluble
213 V H -1.7895 Soluble
214 D H -2.5121 Soluble
215 K H -1.7608 Soluble
1 D L -2.3111 Soluble
2 I L 0.0000 Buried
3 Q L -2.0553 Soluble
4 M L 0.0000 Buried
5 T L -1.1105 Soluble
6 Q L -0.8499 Soluble
7 S L -0.6829 Soluble
8 P L -0.5153 Soluble
9 S L -0.6487 Soluble
10 S L -0.6073 Soluble
11 L L -0.4015 Soluble
12 S L -0.7420 Soluble
13 A L -0.9203 Soluble
14 A L -0.7598 Soluble
15 V L -0.0812 Soluble
16 G L -0.7158 Soluble
17 D L -1.6458 Soluble
18 R L -2.2675 Soluble
19 V L 0.0000 Buried
20 T L -0.6179 Soluble
21 I L 0.0000 Buried
22 T L -0.7480 Soluble
23 C L 0.0000 Buried
24 Q L -2.1671 Soluble
25 A L 0.0000 Buried
26 S L -1.9917 Soluble
27 Q L -2.3222 Soluble
28 D L -2.2457 Soluble
29 I L 0.0000 Buried
36 V L 0.4097 Aggregation-prone
37 N L 0.1044 Aggregation-prone
38 Y L 0.5214 Aggregation-prone
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.9945 Soluble
46 P L -1.4737 Soluble
47 G L -1.8004 Soluble
48 K L -2.6181 Soluble
49 A L -1.7078 Soluble
50 P L 0.0000 Buried
51 K L -1.7403 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.4144 Aggregation-prone
56 V L 0.7842 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.3279 Soluble
66 N L -0.3658 Soluble
67 L L 0.0567 Aggregation-prone
68 E L -0.5930 Soluble
69 T L -0.4360 Soluble
70 G L -0.5546 Soluble
71 V L 0.0000 Buried
72 P L -0.3952 Soluble
74 S L -0.4467 Soluble
75 R L -0.7573 Soluble
76 F L 0.0000 Buried
77 S L -0.3654 Soluble
78 G L 0.0000 Buried
79 S L -0.6608 Soluble
80 G L -0.8466 Soluble
83 S L -0.7176 Soluble
84 G L -1.0298 Soluble
85 T L -1.7894 Soluble
86 D L -2.2455 Soluble
87 F L 0.0000 Buried
88 S L -0.7089 Soluble
89 L L 0.0000 Buried
90 T L -0.5809 Soluble
91 I L 0.0000 Buried
92 S L -1.2929 Soluble
93 S L -1.0911 Soluble
94 L L 0.0000 Buried
95 Q L -1.2185 Soluble
96 P L -2.0481 Soluble
97 E L -2.6783 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.7225 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L -0.0268 Soluble
108 D L -1.0265 Soluble
109 N L -1.1815 Soluble
114 L L -0.4303 Soluble
115 P L -0.7128 Soluble
116 L L 0.0000 Buried
117 T L -0.7241 Soluble
118 F L -0.2634 Soluble
119 G L -0.8082 Soluble
120 G L -1.0612 Soluble
121 G L -0.8080 Soluble
122 T L 0.0000 Buried
123 K L -1.1031 Soluble
124 V L 0.0000 Buried
125 E L -1.2377 Soluble
126 I L 0.0000 Buried
127 K L -1.4155 Soluble
128 R L -1.0274 Soluble
129 T L -0.1661 Soluble
130 V L 0.3635 Aggregation-prone
131 A L 0.1420 Aggregation-prone
132 A L -0.1190 Soluble
133 P L 0.0000 Buried
134 S L -0.0965 Soluble
135 V L 0.0000 Buried
136 F L 0.2291 Aggregation-prone
137 I L 0.1892 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6779 Soluble
140 P L -0.8649 Soluble
141 S L -1.5517 Soluble
142 D L -2.7859 Soluble
143 E L -2.8891 Soluble
144 Q L 0.0000 Buried
145 L L -1.7658 Soluble
146 K L -2.6134 Soluble
147 S L -1.4248 Soluble
148 G L -1.0915 Soluble
149 T L -0.8933 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7059 Soluble
159 F L 0.0000 Buried
160 Y L -0.8478 Soluble
161 P L -1.3182 Soluble
162 R L -1.9745 Soluble
163 E L -2.7725 Soluble
164 A L -2.1248 Soluble
165 K L -2.3546 Soluble
166 V L -1.1055 Soluble
167 Q L -0.4918 Soluble
168 W L 0.0000 Buried
169 K L -0.5336 Soluble
170 V L 0.0000 Buried
171 D L -2.0389 Soluble
172 N L -1.5104 Soluble
173 A L -0.3473 Soluble
174 L L 0.6931 Aggregation-prone
175 Q L -0.2372 Soluble
176 S L -0.7057 Soluble
177 G L -1.2816 Soluble
178 N L -1.5154 Soluble
179 S L -1.7318 Soluble
180 Q L -2.2064 Soluble
181 E L -2.2645 Soluble
182 S L -0.9282 Soluble
183 V L -0.3421 Soluble
184 T L -0.5166 Soluble
185 E L -1.2446 Soluble
186 Q L 0.0000 Buried
187 D L -2.0343 Soluble
188 S L -2.5518 Soluble
189 K L -2.9752 Soluble
190 D L -2.4243 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.9918 Soluble
199 L L 0.0000 Buried
200 T L -0.5674 Soluble
201 L L -0.6435 Soluble
202 S L -1.1971 Soluble
203 K L -2.0009 Soluble
204 A L -1.8818 Soluble
205 D L -3.1923 Soluble
206 Y L 0.0000 Buried
207 E L -4.2514 Soluble
208 K L -3.8503 Soluble
209 H L -3.2929 Soluble
210 K L -4.0317 Soluble
211 V L -2.5436 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7021 Soluble
216 V L 0.0000 Buried
217 T L -1.2092 Soluble
218 H L 0.0000 Buried
219 Q L -1.7609 Soluble
220 G L -0.7067 Soluble
221 L L -0.3272 Soluble
222 S L -0.5192 Soluble
223 S L -0.4281 Soluble
224 P L -0.5453 Soluble
225 V L 0.0526 Aggregation-prone
226 T L -0.4857 Soluble
227 K L -0.8288 Soluble
228 S L -0.9968 Soluble
229 F L 0.0000 Buried
230 N L -2.9959 Soluble
231 R L -3.0736 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5461 2.503 CSV PDB
4.5 -0.5901 2.503 CSV PDB
5.0 -0.6402 2.503 CSV PDB
5.5 -0.6871 2.503 CSV PDB
6.0 -0.7209 2.503 CSV PDB
6.5 -0.7343 2.503 CSV PDB
7.0 -0.7286 2.503 CSV PDB
7.5 -0.7109 2.503 CSV PDB
8.0 -0.6866 2.503 CSV PDB
8.5 -0.6566 2.5029 CSV PDB
9.0 -0.6201 2.5029 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH