Anbenitamab / 1fvd

A4DAB016_1fvd_D_C | Source PDB: 1fvd | Chains: D, C | Done
98.68
Yes
10 Å
Yes
Avg A4D Score
-0.7552
Max Score
1.7595
Min Score
-3.542
Total Score
-330.03
Score CDR
-0.5605
Score non-CDR
-0.7787

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTLYLQMNSLRAEDTAVYYCSRWGGDGFYAMDVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLESGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.542
Maximum Score 1.7595
Average Score -0.7552
Total Score -330.0262

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.0623 Soluble
2 V D -1.3185 Soluble
3 Q D -1.4400 Soluble
4 L D 0.0000 Buried
5 V D 0.5702 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.4934 Soluble
8 G D -0.9477 Soluble
9 G D -0.4615 Soluble
11 G D -0.1550 Soluble
12 L D 0.0834 Aggregation-prone
13 V D -0.5680 Soluble
14 Q D -1.5306 Soluble
15 P D -1.6185 Soluble
16 G D -1.3509 Soluble
17 G D -1.0617 Soluble
18 S D -1.0942 Soluble
19 L D -1.1359 Soluble
20 R D -2.0020 Soluble
21 L D 0.0000 Buried
22 S D -0.3758 Soluble
23 C D 0.0000 Buried
24 A D -0.3228 Soluble
25 A D 0.0000 Buried
26 S D -1.0088 Soluble
27 G D -1.3621 Soluble
28 F D -1.3352 Soluble
29 N D -2.1668 Soluble
30 I D 0.0000 Buried
35 K D -2.4399 Soluble
36 D D -2.8633 Soluble
37 T D -1.7768 Soluble
38 Y D -0.6600 Soluble
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.8700 Soluble
45 A D -1.2399 Soluble
46 P D -0.9235 Soluble
47 G D -1.4781 Soluble
48 K D -2.4359 Soluble
49 G D -1.7442 Soluble
50 L D 0.0000 Buried
51 E D -1.3336 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 R D -0.5438 Soluble
56 I D 0.0000 Buried
57 Y D -0.5200 Soluble
58 P D 0.0000 Buried
59 T D -1.2540 Soluble
62 N D -1.2905 Soluble
63 G D -0.4440 Soluble
64 Y D 0.4007 Aggregation-prone
65 T D -0.4703 Soluble
66 R D -1.9731 Soluble
67 Y D -1.8917 Soluble
68 A D -1.9134 Soluble
69 D D -2.7134 Soluble
70 S D -1.8169 Soluble
71 V D 0.0000 Buried
72 K D -2.8993 Soluble
74 G D -1.7676 Soluble
75 R D -1.5352 Soluble
76 F D 0.0000 Buried
77 T D -1.0423 Soluble
78 I D 0.0000 Buried
79 S D -0.0832 Soluble
80 A D -0.7165 Soluble
81 D D -1.3405 Soluble
82 T D -1.5462 Soluble
83 S D -1.5658 Soluble
84 K D -2.2446 Soluble
85 N D -1.7658 Soluble
86 T D -0.9196 Soluble
87 L D 0.0000 Buried
88 Y D -0.4349 Soluble
89 L D 0.0000 Buried
90 Q D -1.1157 Soluble
91 M D 0.0000 Buried
92 N D -1.2979 Soluble
93 S D -1.1891 Soluble
94 L D 0.0000 Buried
95 R D -2.2201 Soluble
96 A D -1.6641 Soluble
97 E D -2.2388 Soluble
98 D D 0.0000 Buried
99 T D -0.6801 Soluble
100 A D 0.0000 Buried
101 V D 0.3094 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 S D 0.0000 Buried
106 R D -0.7342 Soluble
107 W D 0.0000 Buried
108 G D 0.0000 Buried
109 G D -0.9141 Soluble
110 D D -1.7344 Soluble
111 G D 0.0000 Buried
112 F D 0.9685 Aggregation-prone
113 Y D 0.7704 Aggregation-prone
114 A D 0.0000 Buried
115 M D 0.0000 Buried
116 D D -0.4488 Soluble
117 V D 0.0000 Buried
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.4503 Soluble
121 G D -0.5141 Soluble
122 T D 0.0017 Aggregation-prone
123 L D 0.3701 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3717 Soluble
126 V D 0.0000 Buried
127 S D -0.8867 Soluble
128 S D -0.6703 Soluble
129 A D -0.4863 Soluble
130 S D -0.6521 Soluble
131 T D -0.8909 Soluble
132 K D -1.5013 Soluble
133 G D -1.4628 Soluble
134 P D 0.0000 Buried
135 S D -0.5307 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.2153 Soluble
139 L D 0.0000 Buried
140 A D -1.2345 Soluble
141 P D 0.0000 Buried
142 S D -1.5380 Soluble
143 S D -1.9669 Soluble
144 K D -2.3256 Soluble
145 S D -1.3979 Soluble
146 T D -0.8392 Soluble
147 S D -0.6959 Soluble
148 G D -1.1172 Soluble
149 G D -1.1351 Soluble
150 T D -0.6295 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D -0.3221 Soluble
159 D D -0.5329 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.4618 Soluble
164 P D -0.6136 Soluble
165 V D -0.5698 Soluble
166 T D -0.4524 Soluble
167 V D -0.3062 Soluble
168 S D -0.3208 Soluble
169 W D 0.0000 Buried
170 N D -0.8583 Soluble
171 S D -0.6976 Soluble
172 G D -0.5813 Soluble
173 A D -0.2672 Soluble
174 L D -0.0068 Soluble
175 T D -0.1662 Soluble
176 S D -0.1603 Soluble
177 G D -0.1664 Soluble
178 V D 0.1878 Aggregation-prone
179 H D -0.3337 Soluble
180 T D -0.1800 Soluble
181 F D 0.0000 Buried
182 P D -0.3256 Soluble
183 A D 0.2428 Aggregation-prone
184 V D 0.6601 Aggregation-prone
185 L D 1.4759 Aggregation-prone
186 Q D 0.4405 Aggregation-prone
187 S D -0.0685 Soluble
188 S D -0.1862 Soluble
189 G D 0.0878 Aggregation-prone
190 L D 0.1673 Aggregation-prone
191 Y D 0.4366 Aggregation-prone
192 S D 0.0000 Buried
193 L D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.0982 Soluble
199 V D 0.0000 Buried
200 P D -0.5489 Soluble
201 S D -0.9649 Soluble
202 S D -0.9248 Soluble
203 S D 0.0000 Buried
204 L D -1.1966 Soluble
205 G D -1.4090 Soluble
206 T D -0.8770 Soluble
207 Q D -1.5500 Soluble
208 T D -1.3673 Soluble
209 Y D 0.0000 Buried
210 I D -1.5033 Soluble
211 C D 0.0000 Buried
212 N D 0.0000 Buried
213 V D 0.0000 Buried
214 N D -1.7208 Soluble
215 H D 0.0000 Buried
216 K D -2.2481 Soluble
217 P D -1.4433 Soluble
218 S D -1.7594 Soluble
219 N D -2.3902 Soluble
220 T D -2.1631 Soluble
221 K D -2.5565 Soluble
222 V D -1.7704 Soluble
223 D D -2.5655 Soluble
224 K D -2.1464 Soluble
225 K D -2.6806 Soluble
226 V D 0.0000 Buried
227 E D -2.8973 Soluble
228 P D -2.2687 Soluble
229 K D -2.4033 Soluble
230 S D -1.2417 Soluble
231 C D -0.7057 Soluble
1 D C -2.2749 Soluble
2 I C 0.0000 Buried
3 Q C -2.2021 Soluble
4 M C 0.0000 Buried
5 T C -1.3711 Soluble
6 Q C 0.0000 Buried
7 S C -0.7704 Soluble
8 P C -0.7182 Soluble
9 S C -1.0292 Soluble
10 S C -0.9773 Soluble
11 L C -0.7256 Soluble
12 S C -0.9043 Soluble
13 A C 0.0000 Buried
14 S C -0.8572 Soluble
15 V C -0.2527 Soluble
16 G C -0.8824 Soluble
17 D C -1.9311 Soluble
18 R C -2.4221 Soluble
19 V C 0.0000 Buried
20 T C -0.6788 Soluble
21 I C 0.0000 Buried
22 T C -0.7630 Soluble
23 C C 0.0000 Buried
24 R C -2.8057 Soluble
25 A C 0.0000 Buried
26 S C -2.2807 Soluble
27 Q C -2.9007 Soluble
28 D C -2.8235 Soluble
29 V C 0.0000 Buried
36 N C -1.6432 Soluble
37 T C -0.6677 Soluble
38 A C 0.0891 Aggregation-prone
39 V C 0.0000 Buried
40 A C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.2223 Soluble
45 K C -1.5766 Soluble
46 P C -1.3641 Soluble
47 G C -1.4998 Soluble
48 K C -2.2424 Soluble
49 A C -1.5399 Soluble
50 P C 0.0000 Buried
51 K C -1.4041 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C 1.1097 Aggregation-prone
56 S C 0.6814 Aggregation-prone
57 A C 0.0000 Buried
65 S C 0.4930 Aggregation-prone
66 F C 1.7595 Aggregation-prone
67 L C 0.9189 Aggregation-prone
68 E C 0.0116 Aggregation-prone
69 S C -0.2988 Soluble
70 G C -0.6332 Soluble
71 V C 0.0000 Buried
72 P C -0.4786 Soluble
74 S C -0.4950 Soluble
75 R C -0.7051 Soluble
76 F C 0.0000 Buried
77 S C 0.0626 Aggregation-prone
78 G C 0.0000 Buried
79 S C -0.6859 Soluble
80 R C -1.7814 Soluble
83 S C -1.3728 Soluble
84 G C -1.7865 Soluble
85 T C -2.1516 Soluble
86 D C -1.8951 Soluble
87 F C 0.0000 Buried
88 T C -0.7611 Soluble
89 L C 0.0000 Buried
90 T C -0.6116 Soluble
91 I C 0.0000 Buried
92 S C -1.3004 Soluble
93 S C -1.1358 Soluble
94 L C 0.0000 Buried
95 Q C -0.7643 Soluble
96 P C -1.5619 Soluble
97 E C -2.0778 Soluble
98 D C 0.0000 Buried
99 F C -1.2988 Soluble
100 A C 0.0000 Buried
101 T C 0.0000 Buried
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.0000 Buried
108 Y C 0.4944 Aggregation-prone
109 T C 0.1742 Aggregation-prone
114 T C -0.4310 Soluble
115 P C -0.8321 Soluble
116 P C 0.0000 Buried
117 T C -0.7037 Soluble
118 F C -0.5632 Soluble
119 G C 0.0000 Buried
120 Q C -1.8388 Soluble
121 G C -1.4365 Soluble
122 T C 0.0000 Buried
123 K C -2.0402 Soluble
124 V C 0.0000 Buried
125 E C -1.4474 Soluble
126 I C 0.0000 Buried
127 K C -1.2082 Soluble
128 R C -0.9174 Soluble
129 T C -0.0306 Soluble
130 V C 0.3665 Aggregation-prone
131 A C -0.1907 Soluble
132 A C -0.1137 Soluble
133 P C 0.0000 Buried
134 S C -0.2155 Soluble
135 V C 0.0000 Buried
136 F C 0.0000 Buried
137 I C 0.0319 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.6034 Soluble
140 P C -0.8315 Soluble
141 S C -1.8526 Soluble
142 D C -3.0258 Soluble
143 E C -3.1286 Soluble
144 Q C 0.0000 Buried
145 L C -2.3490 Soluble
146 K C -2.8729 Soluble
147 S C -1.7497 Soluble
148 G C -1.4921 Soluble
149 T C -1.2395 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -1.1256 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.8071 Soluble
162 R C -3.1312 Soluble
163 E C -3.2958 Soluble
164 A C -2.4549 Soluble
165 K C -2.6334 Soluble
166 V C 0.0000 Buried
167 Q C -0.9957 Soluble
168 W C 0.0000 Buried
169 K C -0.6596 Soluble
170 V C 0.0000 Buried
171 D C -1.8646 Soluble
172 N C -1.4822 Soluble
173 A C -0.2507 Soluble
174 L C 0.5340 Aggregation-prone
175 Q C -0.3422 Soluble
176 S C -0.5518 Soluble
177 G C -0.8946 Soluble
178 N C -0.7663 Soluble
179 S C -1.0743 Soluble
180 Q C -1.1380 Soluble
181 E C -1.8689 Soluble
182 S C -0.9913 Soluble
183 V C -0.8677 Soluble
184 T C -1.1596 Soluble
185 E C -2.2285 Soluble
186 Q C 0.0000 Buried
187 D C -2.2605 Soluble
188 S C -2.4076 Soluble
189 K C -2.9351 Soluble
190 D C -2.6864 Soluble
191 S C -2.1745 Soluble
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.5760 Soluble
199 L C 0.0000 Buried
200 T C -0.3723 Soluble
201 L C -0.6310 Soluble
202 S C -1.0164 Soluble
203 K C -2.0682 Soluble
204 A C -1.7653 Soluble
205 D C -2.3358 Soluble
206 Y C 0.0000 Buried
207 E C -3.4395 Soluble
208 K C -3.5420 Soluble
209 H C -3.0046 Soluble
210 K C -3.2451 Soluble
211 V C -1.5729 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9378 Soluble
216 V C 0.0000 Buried
217 T C -1.2824 Soluble
218 H C 0.0000 Buried
219 Q C -1.7964 Soluble
220 G C -0.3739 Soluble
221 L C -0.2514 Soluble
222 S C -0.4644 Soluble
223 S C -0.4148 Soluble
224 P C -0.6369 Soluble
225 V C -0.0628 Soluble
226 T C -0.5554 Soluble
227 K C -0.7204 Soluble
228 S C -0.6358 Soluble
229 F C -0.8270 Soluble
230 N C -2.1501 Soluble
231 R C -2.3988 Soluble
232 G C -2.0694 Soluble
233 E C -1.8448 Soluble
234 C C -0.2478 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7719 3.4808 CSV PDB
4.5 -0.8194 3.3695 CSV PDB
5.0 -0.8752 3.2382 CSV PDB
5.5 -0.929 3.143 CSV PDB
6.0 -0.9685 3.1258 CSV PDB
6.5 -0.9838 3.112 CSV PDB
7.0 -0.9744 3.1035 CSV PDB
7.5 -0.9483 3.0997 CSV PDB
8.0 -0.913 3.0983 CSV PDB
8.5 -0.8707 3.0977 CSV PDB
9.0 -0.8208 3.0972 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH