Ranibizumab / 6bft

A4DAB218_6bft_H_L | Source PDB: 6bft | Chains: H, L | Done
96.96
Yes
10 Å
Yes
Avg A4D Score
-0.7047
Max Score
1.255
Min Score
-3.6354
Total Score
-303.03
Score CDR
-0.3845
Score non-CDR
-0.7469

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGYDFDNYGMNWVRQAPGKGLEWVGWINTYTGEPTYAADFKRRFTFSLDTSKSTAYLQMNSLRAEDTAVYYCAKYPHYYGSSHWYFDVWGQGTLVTVSSASTKGPSVFPLAPGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCSASQDISNYLNWYQQKPGKAPKVLIYFTDDLHSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYSTVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6354
Maximum Score 1.255
Average Score -0.7047
Total Score -303.0316

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9004 Soluble
2 V H -1.0335 Soluble
3 Q H -0.8022 Soluble
4 L H 0.0000 Buried
5 V H 0.7611 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5501 Soluble
8 G H -1.0320 Soluble
9 G H -0.5959 Soluble
11 G H -0.3785 Soluble
12 L H -0.0751 Soluble
13 V H 0.0000 Buried
14 Q H -1.7530 Soluble
15 P H -1.6024 Soluble
16 G H -1.5773 Soluble
17 G H -1.3308 Soluble
18 S H -1.5894 Soluble
19 L H -1.2827 Soluble
20 R H -2.1013 Soluble
21 L H 0.0000 Buried
22 S H -0.4568 Soluble
23 C H 0.0000 Buried
24 A H -0.1077 Soluble
25 A H 0.0000 Buried
26 S H -1.0785 Soluble
27 G H -1.4313 Soluble
28 Y H -1.4975 Soluble
29 D H -2.3353 Soluble
30 F H 0.0000 Buried
35 D H -1.4501 Soluble
36 N H -1.9051 Soluble
37 Y H -0.9924 Soluble
38 G H -0.2304 Soluble
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.6287 Soluble
44 Q H -0.9238 Soluble
45 A H -1.2486 Soluble
46 P H -0.9808 Soluble
47 G H -1.4737 Soluble
48 K H -2.3689 Soluble
49 G H -1.6377 Soluble
50 L H 0.0000 Buried
51 E H -1.2853 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 W H -0.1372 Soluble
56 I H 0.0000 Buried
57 N H -0.8699 Soluble
58 T H 0.0000 Buried
59 Y H 0.3071 Aggregation-prone
62 T H -0.2818 Soluble
63 G H -0.9507 Soluble
64 E H -1.8539 Soluble
65 P H -1.0699 Soluble
66 T H -0.4465 Soluble
67 Y H -0.6115 Soluble
68 A H 0.0000 Buried
69 A H -1.6080 Soluble
70 D H -2.5886 Soluble
71 F H 0.0000 Buried
72 K H -3.1227 Soluble
74 R H -3.3040 Soluble
75 R H -2.4593 Soluble
76 F H 0.0000 Buried
77 T H -1.2355 Soluble
78 F H 0.0000 Buried
79 S H -0.4499 Soluble
80 L H -0.4834 Soluble
81 D H -0.9842 Soluble
82 T H -1.0493 Soluble
83 S H -1.2594 Soluble
84 K H -1.9637 Soluble
85 S H -1.4461 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.5512 Soluble
91 M H 0.0000 Buried
92 N H -2.2713 Soluble
93 S H -1.6989 Soluble
94 L H 0.0000 Buried
95 R H -2.4475 Soluble
96 A H -1.7249 Soluble
97 E H -2.2280 Soluble
98 D H 0.0000 Buried
99 T H -0.7572 Soluble
100 A H 0.0000 Buried
101 V H 0.1355 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 K H 0.0000 Buried
107 Y H 0.0000 Buried
108 P H 0.0000 Buried
109 H H -0.4959 Soluble
110 Y H 0.9657 Aggregation-prone
111 Y H 1.2550 Aggregation-prone
111A G H 0.1963 Aggregation-prone
112B S H 0.0498 Aggregation-prone
112A S H 0.1889 Aggregation-prone
112 H H 0.4716 Aggregation-prone
113 W H 0.4966 Aggregation-prone
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.5455 Soluble
117 V H -0.1918 Soluble
118 W H -0.4052 Soluble
119 G H 0.0000 Buried
120 Q H -1.3287 Soluble
121 G H -0.6532 Soluble
122 T H -0.1843 Soluble
123 L H 0.0762 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4562 Soluble
126 V H 0.0000 Buried
127 S H -0.7697 Soluble
128 S H -0.7387 Soluble
129 A H -0.4551 Soluble
130 S H -0.6376 Soluble
131 T H -0.7696 Soluble
132 K H -1.6337 Soluble
133 G H -1.7143 Soluble
134 P H -0.7513 Soluble
135 S H -0.4338 Soluble
136 V H 0.0000 Buried
137 F H -0.4800 Soluble
138 P H -0.9426 Soluble
139 L H 0.0000 Buried
140 A H -0.8131 Soluble
141 P H -0.5032 Soluble
142 G H -0.8270 Soluble
143 G H -0.8661 Soluble
144 T H -0.5608 Soluble
145 A H -0.2307 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.6926 Soluble
154 Y H 0.0000 Buried
155 F H -0.4005 Soluble
156 P H 0.0000 Buried
157 E H -0.4690 Soluble
158 P H -0.6704 Soluble
159 V H -0.4036 Soluble
160 T H -0.4704 Soluble
161 V H -0.0880 Soluble
162 S H -0.3697 Soluble
163 W H 0.0000 Buried
164 N H -0.7533 Soluble
165 S H -0.6675 Soluble
166 G H -0.5114 Soluble
167 A H -0.2193 Soluble
168 L H -0.0306 Soluble
169 T H -0.1837 Soluble
170 S H -0.2100 Soluble
171 G H -0.2515 Soluble
172 V H 0.1453 Aggregation-prone
173 H H -0.3186 Soluble
174 T H -0.0104 Soluble
175 F H 0.0000 Buried
176 P H -0.3050 Soluble
177 A H 0.1912 Aggregation-prone
178 V H 0.4146 Aggregation-prone
179 L H 0.9750 Aggregation-prone
180 Q H 0.1732 Aggregation-prone
181 S H -0.1058 Soluble
182 S H -0.2703 Soluble
183 G H 0.0039 Aggregation-prone
184 L H -0.0112 Soluble
185 Y H 0.3157 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1635 Soluble
193 V H 0.0000 Buried
194 P H -0.5769 Soluble
195 S H -0.6067 Soluble
196 S H -0.6861 Soluble
197 S H -0.5483 Soluble
198 L H -0.7684 Soluble
199 G H -0.9387 Soluble
200 T H -0.6484 Soluble
201 Q H -1.0872 Soluble
202 T H -1.0522 Soluble
203 Y H 0.0000 Buried
204 I H -1.3107 Soluble
205 C H 0.0000 Buried
206 N H -1.3946 Soluble
207 V H 0.0000 Buried
208 N H -1.6607 Soluble
209 H H 0.0000 Buried
210 K H -2.7202 Soluble
211 P H -1.6579 Soluble
212 S H -1.9450 Soluble
213 N H -2.5475 Soluble
214 T H -2.0892 Soluble
215 K H -2.5958 Soluble
216 V H -1.5542 Soluble
217 D H -2.4258 Soluble
218 K H -2.0318 Soluble
219 K H -2.3873 Soluble
220 V H 0.0000 Buried
221 E H -2.4490 Soluble
222 P H -1.1788 Soluble
1 D L -1.9378 Soluble
2 I L 0.0000 Buried
3 Q L -2.0048 Soluble
4 M L 0.0000 Buried
5 T L -0.8847 Soluble
6 Q L 0.0000 Buried
7 S L -0.5881 Soluble
8 P L -0.5361 Soluble
9 S L -0.8429 Soluble
10 S L -0.8365 Soluble
11 L L -0.5582 Soluble
12 S L -0.7706 Soluble
13 A L 0.0000 Buried
14 S L -0.9574 Soluble
15 V L -0.1634 Soluble
16 G L -0.9145 Soluble
17 D L -2.0737 Soluble
18 R L -2.4380 Soluble
19 V L 0.0000 Buried
20 T L -0.6756 Soluble
21 I L 0.0000 Buried
22 T L -0.6037 Soluble
23 C L 0.0000 Buried
24 S L -1.4547 Soluble
25 A L 0.0000 Buried
26 S L -1.9200 Soluble
27 Q L -2.5388 Soluble
28 D L -2.6431 Soluble
29 I L 0.0000 Buried
36 S L -1.0194 Soluble
37 N L -0.6949 Soluble
38 Y L 0.3336 Aggregation-prone
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.2740 Soluble
45 K L -1.5548 Soluble
46 P L -1.3887 Soluble
47 G L -1.7769 Soluble
48 K L -2.5289 Soluble
49 A L -1.5760 Soluble
50 P L 0.0000 Buried
51 K L -1.6241 Soluble
52 V L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1044 Soluble
56 F L 0.1795 Aggregation-prone
57 T L 0.0000 Buried
65 D L -2.0431 Soluble
66 D L -1.5316 Soluble
67 L L -0.6159 Soluble
68 H L -0.8161 Soluble
69 S L -0.5382 Soluble
70 G L -0.5260 Soluble
71 V L -0.3951 Soluble
72 P L -0.3757 Soluble
74 S L -0.4676 Soluble
75 R L -0.8106 Soluble
76 F L 0.0000 Buried
77 S L -0.9171 Soluble
78 G L -1.0120 Soluble
79 S L -1.3155 Soluble
80 G L -1.4315 Soluble
83 S L -1.2645 Soluble
84 G L -1.7643 Soluble
85 T L -2.0791 Soluble
86 D L -2.4780 Soluble
87 F L 0.0000 Buried
88 T L -0.8797 Soluble
89 L L 0.0000 Buried
90 T L -0.6325 Soluble
91 I L 0.0000 Buried
92 S L -1.3892 Soluble
93 S L -1.2173 Soluble
94 L L 0.0000 Buried
95 Q L -0.7526 Soluble
96 P L -1.3882 Soluble
97 E L -1.6626 Soluble
98 D L 0.0000 Buried
99 F L -1.1452 Soluble
100 A L 0.0000 Buried
101 T L -0.8728 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 S L 0.0356 Aggregation-prone
109 T L 0.4779 Aggregation-prone
114 V L 1.2262 Aggregation-prone
115 P L 0.2774 Aggregation-prone
116 W L 0.0000 Buried
117 T L -0.6444 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.6083 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.6259 Soluble
124 V L 0.0000 Buried
125 E L -1.2267 Soluble
126 I L 0.0000 Buried
127 K L -1.3707 Soluble
128 R L -0.8878 Soluble
129 T L -0.0861 Soluble
130 V L 0.4135 Aggregation-prone
131 A L 0.1707 Aggregation-prone
132 A L -0.0984 Soluble
133 P L 0.0000 Buried
134 S L -0.1168 Soluble
135 V L 0.0000 Buried
136 F L 0.1667 Aggregation-prone
137 I L 0.1425 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5463 Soluble
140 P L 0.0000 Buried
141 S L -1.3234 Soluble
142 D L -2.5369 Soluble
143 E L -2.1431 Soluble
144 Q L 0.0000 Buried
145 L L -1.8210 Soluble
146 K L -2.5938 Soluble
147 S L -1.5798 Soluble
148 G L -1.1730 Soluble
149 T L -0.9725 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8706 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6577 Soluble
162 R L -2.8207 Soluble
163 E L -3.0290 Soluble
164 A L -2.0858 Soluble
165 K L -2.0043 Soluble
166 V L -0.9447 Soluble
167 Q L -0.6402 Soluble
168 W L 0.0000 Buried
169 K L -0.5700 Soluble
170 V L 0.0000 Buried
171 D L -1.9250 Soluble
172 N L -1.5095 Soluble
173 A L -0.2118 Soluble
174 L L 0.7120 Aggregation-prone
175 Q L -0.1509 Soluble
176 S L -0.4319 Soluble
177 G L -0.8615 Soluble
178 N L -0.7335 Soluble
179 S L -1.0002 Soluble
180 Q L -1.1652 Soluble
181 E L -1.5864 Soluble
182 S L -0.8508 Soluble
183 V L -0.9486 Soluble
184 T L -1.0915 Soluble
185 E L -2.0672 Soluble
186 Q L 0.0000 Buried
187 D L -1.9419 Soluble
188 S L -2.0874 Soluble
189 K L -2.5155 Soluble
190 D L -1.8941 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5479 Soluble
199 L L 0.0000 Buried
200 T L -0.4129 Soluble
201 L L -0.6847 Soluble
202 S L -1.0891 Soluble
203 K L -2.0540 Soluble
204 A L -1.9240 Soluble
205 D L -2.6707 Soluble
206 Y L 0.0000 Buried
207 E L -3.4605 Soluble
208 K L -3.6354 Soluble
209 H L -3.2580 Soluble
210 K L -3.3727 Soluble
211 V L -1.6134 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8282 Soluble
216 V L 0.0000 Buried
217 T L -1.0497 Soluble
218 H L 0.0000 Buried
219 Q L -1.6845 Soluble
220 G L -0.4339 Soluble
221 L L -0.2109 Soluble
222 S L -0.4622 Soluble
223 S L -0.3832 Soluble
224 P L -0.4266 Soluble
225 V L 0.0980 Aggregation-prone
226 T L -0.4275 Soluble
227 K L -0.6359 Soluble
228 S L -0.6344 Soluble
229 F L -1.1061 Soluble
230 N L -2.2969 Soluble
231 R L -2.7581 Soluble
232 G L -2.2469 Soluble
233 E L -2.6046 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6421 1.9449 CSV PDB
4.5 -0.6927 1.8163 CSV PDB
5.0 -0.7522 1.6442 CSV PDB
5.5 -0.8097 1.5508 CSV PDB
6.0 -0.8534 1.4639 CSV PDB
6.5 -0.8747 1.3945 CSV PDB
7.0 -0.8739 1.3523 CSV PDB
7.5 -0.8593 1.3796 CSV PDB
8.0 -0.8368 1.4534 CSV PDB
8.5 -0.8075 1.5327 CSV PDB
9.0 -0.7696 1.6107 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH