Ravagalimab / 6pe9

A4DAB219_6pe9_C_D | Source PDB: 6pe9 | Chains: C, D | Done
98.25
Yes
10 Å
Yes
Avg A4D Score
-0.7212
Max Score
1.2683
Min Score
-3.288
Total Score
-312.3
Score CDR
-0.3934
Score non-CDR
-0.7631

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVKPGGSLRLSCAASGFTFSDYGMNWVRQAPGKGLEWVSYISSGRSNIYYADTVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARSWGYFDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIVMTQSPDSLAVSLGERATINCKSSQSLLNLGNQKNYLTWFQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNDYTYPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) C (Heavy), D (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.288
Maximum Score 1.2683
Average Score -0.7212
Total Score -312.3011

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E C -1.7826 Soluble
2 V C -0.5949 Soluble
3 Q C -1.0958 Soluble
4 L C 0.0000 Buried
5 V C 0.1169 Aggregation-prone
6 E C 0.0000 Buried
7 S C -0.6244 Soluble
8 G C -0.9588 Soluble
9 G C -0.8164 Soluble
11 G C -0.6241 Soluble
12 L C -0.1813 Soluble
13 V C -0.8338 Soluble
14 K C -2.0081 Soluble
15 P C -1.7793 Soluble
16 G C -1.4299 Soluble
17 G C -1.2560 Soluble
18 S C -1.2620 Soluble
19 L C -1.0907 Soluble
20 R C -1.7681 Soluble
21 L C 0.0000 Buried
22 S C -0.5242 Soluble
23 C C 0.0000 Buried
24 A C -0.3482 Soluble
25 A C 0.0000 Buried
26 S C -0.6667 Soluble
27 G C -0.9245 Soluble
28 F C -0.4960 Soluble
29 T C -0.5301 Soluble
30 F C 0.0000 Buried
35 S C -1.2022 Soluble
36 D C -1.3023 Soluble
37 Y C -0.2685 Soluble
38 G C 0.0000 Buried
39 M C 0.0000 Buried
40 N C 0.0000 Buried
41 W C 0.0000 Buried
42 V C 0.0000 Buried
43 R C 0.0000 Buried
44 Q C -0.8844 Soluble
45 A C -1.2344 Soluble
46 P C -1.0265 Soluble
47 G C -1.4668 Soluble
48 K C -2.3332 Soluble
49 G C -1.4951 Soluble
50 L C 0.0000 Buried
51 E C -1.0182 Soluble
52 W C 0.0000 Buried
53 V C 0.0000 Buried
54 S C 0.0000 Buried
55 Y C 0.5553 Aggregation-prone
56 I C 0.0000 Buried
57 S C -0.8397 Soluble
58 S C -1.1960 Soluble
59 G C -1.8867 Soluble
62 R C -2.3905 Soluble
63 S C -1.5763 Soluble
64 N C -1.2515 Soluble
65 I C 0.4213 Aggregation-prone
66 Y C 0.6894 Aggregation-prone
67 Y C -0.2302 Soluble
68 A C 0.0000 Buried
69 D C -2.4104 Soluble
70 T C -1.6994 Soluble
71 V C 0.0000 Buried
72 K C -2.4981 Soluble
74 G C -1.7346 Soluble
75 R C -1.5098 Soluble
76 F C 0.0000 Buried
77 T C -0.6092 Soluble
78 I C 0.0000 Buried
79 S C -0.3038 Soluble
80 R C -0.9803 Soluble
81 D C -1.5083 Soluble
82 N C -1.7928 Soluble
83 A C -1.4132 Soluble
84 K C -2.2592 Soluble
85 N C -1.7093 Soluble
86 S C -1.0989 Soluble
87 L C 0.0000 Buried
88 Y C -0.5705 Soluble
89 L C 0.0000 Buried
90 Q C -1.2227 Soluble
91 M C 0.0000 Buried
92 N C -1.2970 Soluble
93 S C -1.2086 Soluble
94 L C 0.0000 Buried
95 R C -2.2045 Soluble
96 A C -1.7228 Soluble
97 E C -2.1885 Soluble
98 D C 0.0000 Buried
99 T C -0.7341 Soluble
100 A C 0.0000 Buried
101 V C -0.0392 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 A C 0.0000 Buried
106 R C 0.0000 Buried
107 S C 0.3761 Aggregation-prone
108 W C 0.8227 Aggregation-prone
109 G C 0.3261 Aggregation-prone
114 Y C 0.4279 Aggregation-prone
115 F C 0.0000 Buried
116 D C -0.2326 Soluble
117 V C 0.0804 Aggregation-prone
118 W C -0.4116 Soluble
119 G C 0.0000 Buried
120 Q C -1.4692 Soluble
121 G C 0.0000 Buried
122 T C -0.3303 Soluble
123 T C -0.2349 Soluble
124 V C 0.0000 Buried
125 T C -0.5992 Soluble
126 V C 0.0000 Buried
127 S C -1.0577 Soluble
128 S C -0.7951 Soluble
129 A C -0.5314 Soluble
130 S C -0.6638 Soluble
131 T C -0.7690 Soluble
132 K C -1.3207 Soluble
133 G C -1.5205 Soluble
134 P C -0.7304 Soluble
135 S C -0.5016 Soluble
136 V C 0.0000 Buried
137 F C 0.0000 Buried
138 P C -1.2507 Soluble
139 L C 0.0000 Buried
140 A C -1.1842 Soluble
141 P C 0.0000 Buried
142 S C -0.5317 Soluble
143 G C -0.6417 Soluble
144 T C -0.4544 Soluble
145 A C -0.2258 Soluble
146 A C 0.0000 Buried
147 L C 0.0000 Buried
148 G C 0.0000 Buried
149 C C 0.0000 Buried
150 L C 0.0000 Buried
151 V C 0.0000 Buried
152 K C 0.0000 Buried
153 D C -0.4253 Soluble
154 Y C 0.0000 Buried
155 F C 0.0000 Buried
156 P C 0.0000 Buried
157 E C -0.8237 Soluble
158 P C -0.8330 Soluble
159 V C 0.0000 Buried
160 T C -0.6380 Soluble
161 V C -0.4741 Soluble
162 S C -0.3959 Soluble
163 W C 0.0000 Buried
164 N C -0.6269 Soluble
165 S C -0.6409 Soluble
166 G C -0.5077 Soluble
167 A C -0.2088 Soluble
168 L C 0.0652 Aggregation-prone
169 T C -0.1599 Soluble
170 S C -0.1758 Soluble
171 G C -0.2101 Soluble
172 V C 0.1182 Aggregation-prone
173 H C -0.3359 Soluble
174 T C -0.0227 Soluble
175 F C 0.0000 Buried
176 P C -0.2867 Soluble
177 A C 0.2541 Aggregation-prone
178 V C 0.5091 Aggregation-prone
179 L C 1.2683 Aggregation-prone
180 Q C 0.2354 Aggregation-prone
181 S C -0.1739 Soluble
182 S C -0.2215 Soluble
183 G C 0.0477 Aggregation-prone
184 L C 0.1150 Aggregation-prone
185 Y C 0.4127 Aggregation-prone
186 S C 0.0000 Buried
187 L C 0.0000 Buried
188 S C 0.0000 Buried
189 S C 0.0000 Buried
190 V C 0.0000 Buried
191 V C 0.0000 Buried
192 T C -0.1321 Soluble
193 V C 0.0000 Buried
194 P C -0.4926 Soluble
195 S C -0.5109 Soluble
196 S C -0.5855 Soluble
197 S C -0.5605 Soluble
198 L C -0.7456 Soluble
199 G C -0.9547 Soluble
200 T C -0.6581 Soluble
201 Q C -1.0918 Soluble
202 T C -1.0468 Soluble
203 Y C 0.0000 Buried
204 I C -1.3080 Soluble
205 C C 0.0000 Buried
206 N C -1.4597 Soluble
207 V C 0.0000 Buried
208 N C -1.8375 Soluble
209 H C 0.0000 Buried
210 K C -2.7789 Soluble
211 P C -1.6647 Soluble
212 S C -1.8624 Soluble
213 N C -2.4585 Soluble
214 T C -2.0945 Soluble
215 K C -2.6752 Soluble
216 V C -1.7354 Soluble
217 D C -2.6541 Soluble
218 K C -2.4092 Soluble
219 K C -2.6970 Soluble
220 V C 0.0000 Buried
221 E C -2.8889 Soluble
222 P C -1.9685 Soluble
223 K C -2.1986 Soluble
224 S C -0.8265 Soluble
225 C C 0.0174 Aggregation-prone
1 D D -1.4516 Soluble
2 I D 0.0000 Buried
3 V D 0.7076 Aggregation-prone
4 M D 0.0000 Buried
5 T D -0.9132 Soluble
6 Q D 0.0000 Buried
7 S D -1.5094 Soluble
8 P D -1.4666 Soluble
9 D D -2.4858 Soluble
10 S D -1.6287 Soluble
11 L D -0.8903 Soluble
12 A D -0.7549 Soluble
13 V D -1.1840 Soluble
14 S D -1.2934 Soluble
15 L D -0.6039 Soluble
16 G D -1.3666 Soluble
17 E D -2.8465 Soluble
18 R D -2.8331 Soluble
19 A D 0.0000 Buried
20 T D -0.6882 Soluble
21 I D 0.0000 Buried
22 N D -1.9002 Soluble
23 C D 0.0000 Buried
24 K D -2.4922 Soluble
25 S D 0.0000 Buried
26 S D -1.0170 Soluble
27 Q D -1.5515 Soluble
28 S D -0.9156 Soluble
29 L D 0.0000 Buried
30 L D -0.2222 Soluble
31 N D -0.4441 Soluble
32 L D 0.3410 Aggregation-prone
33 G D -0.7636 Soluble
34 N D -1.6960 Soluble
35 Q D -1.6589 Soluble
36 K D -1.2772 Soluble
37 N D -0.4393 Soluble
38 Y D 0.0945 Aggregation-prone
39 L D 0.0000 Buried
40 T D 0.0000 Buried
41 W D 0.0000 Buried
42 F D 0.0000 Buried
43 Q D 0.0000 Buried
44 Q D 0.0000 Buried
45 K D -1.6954 Soluble
46 P D -1.4152 Soluble
47 G D -1.5782 Soluble
48 Q D -2.0048 Soluble
49 P D -1.3598 Soluble
50 P D 0.0000 Buried
51 K D -1.3106 Soluble
52 L D 0.0000 Buried
53 L D 0.0000 Buried
54 I D 0.0000 Buried
55 Y D -0.0670 Soluble
56 W D 0.1473 Aggregation-prone
57 A D 0.0000 Buried
65 S D -0.5010 Soluble
66 T D -0.4854 Soluble
67 R D -1.4428 Soluble
68 E D -1.0951 Soluble
69 S D -0.7853 Soluble
70 G D -0.9444 Soluble
71 V D -1.0576 Soluble
72 P D -1.3121 Soluble
74 D D -2.2082 Soluble
75 R D -1.6995 Soluble
76 F D 0.0000 Buried
77 S D -0.8815 Soluble
78 G D -0.4802 Soluble
79 S D -0.7047 Soluble
80 G D -1.2586 Soluble
83 S D -1.0397 Soluble
84 G D -1.1818 Soluble
85 T D -1.7242 Soluble
86 D D -2.5308 Soluble
87 F D 0.0000 Buried
88 T D -1.1765 Soluble
89 L D 0.0000 Buried
90 T D -0.7550 Soluble
91 I D 0.0000 Buried
92 S D -2.0774 Soluble
93 S D -1.8042 Soluble
94 L D 0.0000 Buried
95 Q D -1.4834 Soluble
96 A D -1.5075 Soluble
97 E D -1.8947 Soluble
98 D D 0.0000 Buried
99 V D 0.0000 Buried
100 A D 0.0000 Buried
101 V D -0.9401 Soluble
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 Q D 0.0000 Buried
106 N D 0.0000 Buried
107 D D 0.3342 Aggregation-prone
108 Y D 0.4817 Aggregation-prone
109 T D 0.2350 Aggregation-prone
114 Y D 0.4623 Aggregation-prone
115 P D -0.2968 Soluble
116 L D 0.0000 Buried
117 T D 0.0342 Aggregation-prone
118 F D 0.0709 Aggregation-prone
119 G D 0.0000 Buried
120 Q D -1.4787 Soluble
121 G D 0.0000 Buried
122 T D 0.0000 Buried
123 K D -2.0499 Soluble
124 L D 0.0000 Buried
125 E D -1.6362 Soluble
126 I D 0.0000 Buried
127 K D -1.6727 Soluble
128 R D -0.9689 Soluble
129 T D 0.0605 Aggregation-prone
130 V D 1.0177 Aggregation-prone
131 A D 0.3942 Aggregation-prone
132 A D 0.0360 Aggregation-prone
133 P D 0.0000 Buried
134 S D -0.2271 Soluble
135 V D 0.0000 Buried
136 F D 0.0871 Aggregation-prone
137 I D 0.0279 Aggregation-prone
138 F D 0.0000 Buried
139 P D -0.4073 Soluble
140 P D 0.0000 Buried
141 S D -1.8011 Soluble
142 D D -3.1152 Soluble
143 E D -2.9343 Soluble
144 Q D 0.0000 Buried
145 L D -2.3154 Soluble
146 K D -2.8268 Soluble
147 S D -1.8522 Soluble
148 G D -1.3787 Soluble
149 T D -0.9306 Soluble
150 A D 0.0000 Buried
151 S D 0.0000 Buried
152 V D 0.0000 Buried
153 V D 0.0000 Buried
154 C D 0.0000 Buried
155 L D 0.0000 Buried
156 L D 0.0000 Buried
157 N D 0.0000 Buried
158 N D -0.8082 Soluble
159 F D 0.0000 Buried
160 Y D 0.0000 Buried
161 P D -1.7641 Soluble
162 R D -3.2880 Soluble
163 E D -3.2856 Soluble
164 A D -2.3540 Soluble
165 K D -2.5421 Soluble
166 V D -1.1775 Soluble
167 Q D -0.9417 Soluble
168 W D 0.0000 Buried
169 K D -0.6401 Soluble
170 V D 0.0000 Buried
171 D D -1.4332 Soluble
172 N D -1.3249 Soluble
173 A D -0.2716 Soluble
174 L D 0.6309 Aggregation-prone
175 Q D -0.2731 Soluble
176 S D -0.6493 Soluble
177 G D -1.2268 Soluble
178 N D -1.4888 Soluble
179 S D -1.4808 Soluble
180 Q D -1.5571 Soluble
181 E D -1.5323 Soluble
182 S D -0.8272 Soluble
183 V D -0.9616 Soluble
184 T D -1.2019 Soluble
185 E D -2.3783 Soluble
186 Q D 0.0000 Buried
187 D D -2.0192 Soluble
188 S D -2.2273 Soluble
189 K D -2.4362 Soluble
190 D D -1.6726 Soluble
191 S D 0.0000 Buried
192 T D 0.0000 Buried
193 Y D 0.0000 Buried
194 S D 0.0000 Buried
195 L D 0.0000 Buried
196 S D 0.0000 Buried
197 S D 0.0000 Buried
198 T D -0.6938 Soluble
199 L D 0.0000 Buried
200 T D -0.4863 Soluble
201 L D -0.6817 Soluble
202 S D -1.2111 Soluble
203 K D -2.1242 Soluble
204 A D -1.7712 Soluble
205 D D -2.3783 Soluble
206 Y D 0.0000 Buried
207 E D -3.0978 Soluble
208 K D -3.2745 Soluble
209 H D -2.5128 Soluble
210 K D -2.2885 Soluble
211 V D -0.6039 Soluble
212 Y D 0.0000 Buried
213 A D 0.0000 Buried
214 C D 0.0000 Buried
215 E D -1.0541 Soluble
216 V D 0.0000 Buried
217 T D -1.2425 Soluble
218 H D 0.0000 Buried
219 Q D -1.6530 Soluble
220 G D -0.2251 Soluble
221 L D -0.1817 Soluble
222 S D -0.4545 Soluble
223 S D -0.3977 Soluble
224 P D -0.6026 Soluble
225 V D -0.0649 Soluble
226 T D -0.6326 Soluble
227 K D -0.9374 Soluble
228 S D -0.5561 Soluble
229 F D -0.6263 Soluble
230 N D -1.3377 Soluble
231 R D -1.9257 Soluble
232 G D -1.6534 Soluble
233 E D -1.9224 Soluble
234 C D -0.5523 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7092 3.4784 CSV PDB
4.5 -0.7565 3.4199 CSV PDB
5.0 -0.8114 3.3559 CSV PDB
5.5 -0.8628 3.2901 CSV PDB
6.0 -0.8984 3.2236 CSV PDB
6.5 -0.9088 3.1572 CSV PDB
7.0 -0.8941 3.0914 CSV PDB
7.5 -0.8629 3.028 CSV PDB
8.0 -0.8227 2.9702 CSV PDB
8.5 -0.776 2.9231 CSV PDB
9.0 -0.7222 2.8913 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH