Tomuzotuximab / 5id1

A4DAB272_5id1_D_C | Source PDB: 5id1 | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6734
Max Score
1.9815
Min Score
-3.7033
Total Score
-285.51
Score CDR
-0.2951
Score non-CDR
-0.7194

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7033
Maximum Score 1.9815
Average Score -0.6734
Total Score -285.5128

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.3918 Soluble
2 V D -0.7195 Soluble
3 Q D -1.3502 Soluble
4 L D 0.0000 Buried
5 K D -2.2698 Soluble
6 Q D 0.0000 Buried
7 S D -0.7721 Soluble
8 G D -0.9421 Soluble
9 P D -0.4883 Soluble
11 G D -0.4648 Soluble
12 L D -0.1717 Soluble
13 V D 0.0000 Buried
14 Q D -1.7372 Soluble
15 P D -1.4580 Soluble
16 S D -1.4436 Soluble
17 Q D -1.8192 Soluble
18 S D -1.4592 Soluble
19 L D 0.0000 Buried
20 S D -0.5727 Soluble
21 I D 0.0000 Buried
22 T D -0.5182 Soluble
23 C D 0.0000 Buried
24 T D -1.1231 Soluble
25 V D 0.0000 Buried
26 S D -1.1507 Soluble
27 G D -0.9305 Soluble
28 F D -0.5060 Soluble
29 S D -0.8930 Soluble
30 L D 0.0000 Buried
35 T D -0.9273 Soluble
36 N D -0.8518 Soluble
37 Y D 0.4800 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4337 Soluble
45 S D -0.9587 Soluble
46 P D -1.1504 Soluble
47 G D -1.7565 Soluble
48 K D -2.2399 Soluble
49 G D -1.1597 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5176 Soluble
58 S D -0.6418 Soluble
59 G D -1.2445 Soluble
63 G D -1.5554 Soluble
64 N D -1.8710 Soluble
65 T D -1.3373 Soluble
66 D D -1.0518 Soluble
67 Y D -0.3786 Soluble
68 N D 0.0000 Buried
69 T D -0.2958 Soluble
70 P D -0.3595 Soluble
71 F D 0.0000 Buried
72 T D -0.4559 Soluble
74 S D -0.5234 Soluble
75 R D -0.8549 Soluble
76 L D 0.0000 Buried
77 S D -0.9504 Soluble
78 I D 0.0000 Buried
79 N D -1.7210 Soluble
80 K D -1.4650 Soluble
81 D D -1.8111 Soluble
82 N D -2.2617 Soluble
83 S D -1.7006 Soluble
84 K D -2.3796 Soluble
85 S D -1.6065 Soluble
86 Q D -1.5511 Soluble
87 V D 0.0000 Buried
88 F D -0.2939 Soluble
89 F D 0.0000 Buried
90 K D -1.2967 Soluble
91 M D 0.0000 Buried
92 N D -1.3463 Soluble
93 S D -1.3174 Soluble
94 L D 0.0000 Buried
95 Q D -1.8407 Soluble
96 S D -1.4354 Soluble
97 N D -1.9757 Soluble
98 D D 0.0000 Buried
99 T D -0.5613 Soluble
100 A D 0.0000 Buried
101 I D 0.5957 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0733 Aggregation-prone
109 T D 1.1840 Aggregation-prone
110 Y D 1.8792 Aggregation-prone
111 Y D 1.9815 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.6018 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.2488 Aggregation-prone
117 Y D 0.2240 Aggregation-prone
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.7732 Soluble
121 G D -0.9105 Soluble
122 T D 0.0000 Buried
123 L D 0.2817 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3474 Soluble
126 V D 0.0000 Buried
127 S D -0.7016 Soluble
128 A D -0.5440 Soluble
129 A D -0.3486 Soluble
130 S D -0.5610 Soluble
131 T D -0.6467 Soluble
132 K D -1.2924 Soluble
133 G D -1.4737 Soluble
134 P D -0.6668 Soluble
135 S D -0.2994 Soluble
136 V D 0.0000 Buried
137 F D -0.4947 Soluble
138 P D 0.0000 Buried
139 L D 0.0000 Buried
140 A D -1.0234 Soluble
141 G D -0.6359 Soluble
142 T D -0.4867 Soluble
143 A D -0.2318 Soluble
144 A D 0.0000 Buried
145 L D 0.0000 Buried
146 G D 0.0000 Buried
147 C D 0.0000 Buried
148 L D 0.0000 Buried
149 V D 0.0000 Buried
150 K D 0.0000 Buried
151 D D -0.4020 Soluble
152 Y D 0.0000 Buried
153 F D 0.0000 Buried
154 P D 0.0000 Buried
155 E D -0.4527 Soluble
156 P D -0.6679 Soluble
157 V D -0.8197 Soluble
158 T D -0.7598 Soluble
159 V D -0.3228 Soluble
160 S D -0.4363 Soluble
161 W D 0.0000 Buried
162 N D -0.8221 Soluble
163 S D -0.6391 Soluble
164 G D -0.4491 Soluble
165 A D -0.1995 Soluble
166 L D 0.0234 Aggregation-prone
167 T D -0.1351 Soluble
168 S D -0.1683 Soluble
169 G D -0.2544 Soluble
170 V D 0.2037 Aggregation-prone
171 H D 0.0000 Buried
172 T D 0.0380 Aggregation-prone
173 F D 0.0000 Buried
174 P D -0.3298 Soluble
175 A D 0.1910 Aggregation-prone
176 V D 0.5056 Aggregation-prone
177 L D 1.1313 Aggregation-prone
178 Q D 0.2759 Aggregation-prone
179 S D -0.0656 Soluble
180 S D -0.2134 Soluble
181 G D 0.0451 Aggregation-prone
182 L D 0.1379 Aggregation-prone
183 Y D 0.3859 Aggregation-prone
184 S D 0.0000 Buried
185 L D 0.0000 Buried
186 S D 0.0000 Buried
187 S D 0.0000 Buried
188 V D 0.0000 Buried
189 V D 0.0000 Buried
190 T D -0.1206 Soluble
191 V D 0.0000 Buried
192 P D -0.4893 Soluble
193 S D -0.5152 Soluble
194 S D -0.6153 Soluble
195 S D -0.6231 Soluble
196 L D -0.8001 Soluble
197 G D -0.9555 Soluble
198 T D -0.7518 Soluble
199 Q D -1.2134 Soluble
200 T D -1.1431 Soluble
201 Y D 0.0000 Buried
202 I D -1.4193 Soluble
203 C D 0.0000 Buried
204 N D -1.6359 Soluble
205 V D 0.0000 Buried
206 N D -2.4821 Soluble
207 H D 0.0000 Buried
208 K D -2.9623 Soluble
209 P D -1.7030 Soluble
210 S D -1.9402 Soluble
211 N D -2.7351 Soluble
212 T D -2.2452 Soluble
213 K D -2.9392 Soluble
214 V D -1.7708 Soluble
215 D D -2.5989 Soluble
216 K D -2.2813 Soluble
217 R D -2.6009 Soluble
218 V D 0.0000 Buried
219 E D -2.4446 Soluble
220 P D -1.7128 Soluble
221 K D -2.3282 Soluble
1 D C -1.4148 Soluble
2 I C 0.0000 Buried
3 L C 0.6814 Aggregation-prone
4 L C -0.1717 Soluble
5 T C -0.2975 Soluble
6 Q C 0.0000 Buried
7 S C 0.1645 Aggregation-prone
8 P C 0.8297 Aggregation-prone
9 V C 1.8620 Aggregation-prone
10 I C 1.3124 Aggregation-prone
11 L C 0.4610 Aggregation-prone
12 S C -0.3413 Soluble
13 V C 0.0000 Buried
14 S C -1.8283 Soluble
15 P C -1.9242 Soluble
16 G C -2.0873 Soluble
17 E C -2.9720 Soluble
18 R C -3.2115 Soluble
19 V C 0.0000 Buried
20 S C -0.9697 Soluble
21 F C 0.0000 Buried
22 S C -0.7759 Soluble
23 C C 0.0000 Buried
24 R C -2.3749 Soluble
25 A C -1.3050 Soluble
26 S C -0.9648 Soluble
27 Q C -1.5030 Soluble
28 S C -1.5046 Soluble
29 I C 0.0000 Buried
36 G C -1.0230 Soluble
37 T C -0.7833 Soluble
38 N C -0.8260 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.3283 Soluble
45 R C -1.9435 Soluble
46 T C -1.6915 Soluble
47 N C -1.8298 Soluble
48 G C -1.5652 Soluble
49 S C -1.0328 Soluble
50 P C 0.0000 Buried
51 R C -0.7334 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7126 Soluble
56 Y C -0.5635 Soluble
57 A C 0.0000 Buried
65 S C -1.1677 Soluble
66 E C -1.7678 Soluble
67 S C -1.0614 Soluble
68 I C -0.4445 Soluble
69 S C -0.3009 Soluble
70 G C -0.4480 Soluble
71 I C -0.2193 Soluble
72 P C -0.3071 Soluble
74 S C -0.5677 Soluble
75 R C -1.1331 Soluble
76 F C 0.0000 Buried
77 S C -0.9030 Soluble
78 G C -0.7322 Soluble
79 S C -0.8206 Soluble
80 G C -1.0730 Soluble
83 S C -0.9321 Soluble
84 G C -1.2732 Soluble
85 T C -1.8381 Soluble
86 D C -2.4109 Soluble
87 F C 0.0000 Buried
88 T C -0.8607 Soluble
89 L C 0.0000 Buried
90 S C -0.9900 Soluble
91 I C 0.0000 Buried
92 N C -2.4843 Soluble
93 S C -2.2751 Soluble
94 V C 0.0000 Buried
95 E C -1.9663 Soluble
96 S C 0.0000 Buried
97 E C -1.9743 Soluble
98 D C 0.0000 Buried
99 I C -1.4273 Soluble
100 A C 0.0000 Buried
101 D C -0.9669 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6317 Soluble
108 N C -1.2209 Soluble
109 N C -0.9421 Soluble
114 W C 0.1598 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C 0.0342 Aggregation-prone
118 F C 0.2485 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0522 Aggregation-prone
121 G C 0.0950 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.3642 Soluble
124 L C 0.0000 Buried
125 E C -1.1655 Soluble
126 L C 0.0000 Buried
127 K C -1.7160 Soluble
128 R C -1.2189 Soluble
129 T C -0.0773 Soluble
130 V C 0.5239 Aggregation-prone
131 A C 0.2275 Aggregation-prone
132 A C -0.0216 Soluble
133 P C 0.0000 Buried
134 S C -0.1681 Soluble
135 V C 0.0000 Buried
136 F C 0.1828 Aggregation-prone
137 I C 0.1496 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5815 Soluble
140 P C -0.6358 Soluble
141 S C 0.0000 Buried
142 D C -2.6121 Soluble
143 E C -1.9997 Soluble
144 Q C 0.0000 Buried
145 L C -1.9783 Soluble
146 K C -2.4775 Soluble
147 S C -1.5392 Soluble
148 G C -1.2530 Soluble
149 T C -0.8282 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.8119 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.5610 Soluble
162 R C -2.7506 Soluble
163 E C -3.0984 Soluble
164 A C -2.2373 Soluble
165 K C -2.3358 Soluble
166 V C -1.1186 Soluble
167 Q C -0.7660 Soluble
168 W C 0.0000 Buried
169 K C -0.8467 Soluble
170 V C 0.0000 Buried
171 D C -2.0821 Soluble
172 N C -1.6899 Soluble
173 A C -0.4989 Soluble
174 L C 0.2085 Aggregation-prone
175 Q C -0.5584 Soluble
176 S C -0.7577 Soluble
177 G C -1.2732 Soluble
178 N C -1.5858 Soluble
179 S C -1.5272 Soluble
180 Q C -1.5925 Soluble
181 E C -1.9936 Soluble
182 S C -1.0507 Soluble
183 V C -0.7975 Soluble
184 T C -0.9943 Soluble
185 E C -1.9838 Soluble
186 Q C 0.0000 Buried
187 D C -1.9288 Soluble
188 S C -2.1931 Soluble
189 K C -2.5438 Soluble
190 D C -1.7963 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C -1.2111 Soluble
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.8443 Soluble
199 L C 0.0000 Buried
200 T C -0.6517 Soluble
201 L C -0.8214 Soluble
202 S C -1.0271 Soluble
203 K C -2.0014 Soluble
204 A C -1.8900 Soluble
205 D C -2.5195 Soluble
206 Y C 0.0000 Buried
207 E C -3.6311 Soluble
208 K C -3.7033 Soluble
209 H C -3.3334 Soluble
210 K C -3.3179 Soluble
211 V C -1.6087 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9322 Soluble
216 V C 0.0000 Buried
217 T C -1.2090 Soluble
218 H C 0.0000 Buried
219 Q C -1.7003 Soluble
220 G C -0.3569 Soluble
221 L C -0.2156 Soluble
222 S C -0.4689 Soluble
223 S C -0.4494 Soluble
224 P C -0.5715 Soluble
225 V C -0.0804 Soluble
226 T C -0.4671 Soluble
227 K C -0.6267 Soluble
228 S C -0.6807 Soluble
229 F C -0.9380 Soluble
230 N C -2.1616 Soluble
231 R C -2.5019 Soluble
232 G C -1.5779 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6839 2.6295 CSV PDB
4.5 -0.7167 2.5783 CSV PDB
5.0 -0.7561 2.5108 CSV PDB
5.5 -0.7939 2.4355 CSV PDB
6.0 -0.8205 2.3572 CSV PDB
6.5 -0.8281 2.278 CSV PDB
7.0 -0.8161 2.1987 CSV PDB
7.5 -0.7906 2.1985 CSV PDB
8.0 -0.7578 2.1979 CSV PDB
8.5 -0.7199 2.1962 CSV PDB
9.0 -0.6772 2.1912 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH