Tamtuvetmab / 1bfo

A4DAB255_1bfo_H_G | Source PDB: 1bfo | Chains: H, G | Done
96.93
Yes
10 Å
Yes
Avg A4D Score
-0.6817
Max Score
1.6452
Min Score
-3.3783
Total Score
-293.14
Score CDR
-1.0005
Score non-CDR
-0.6417

Calculation Parameters

Chain Sequence(s) EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTTEYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVSSAQTTAPSVYPLAPGCGDTTSSTVTLGCLVKGYFPEPVTVTWNSGALSSDVHTFPAVLQSGLYTLTSSVTSSTWPSQTVTCNVAHPASSTKVDKKVDIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPSRFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKRANAAPTVSIFPPSTEQLATGGASVVCLMNKFYPRDISVKWKIDGTERNGVLNSVTDQDSADSTYSMSSTLSLTKADYQSHNLYTCQVVHKTSSSPVVAKNFNRNEC input.pdb
Selected Chain(s) H (Heavy), G (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3783
Maximum Score 1.6452
Average Score -0.6817
Total Score -293.145

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.2538 Soluble
2 V H 0.1414 Aggregation-prone
3 K H -0.9021 Soluble
4 L H 0.0000 Buried
5 L H 0.6260 Aggregation-prone
6 E H 0.1565 Aggregation-prone
7 S H -0.4495 Soluble
8 G H -0.8697 Soluble
9 G H -0.7084 Soluble
11 G H -0.0197 Soluble
12 L H -0.1212 Soluble
13 V H -0.7240 Soluble
14 Q H -1.6800 Soluble
15 P H -1.6509 Soluble
16 G H -1.3607 Soluble
17 G H -1.0518 Soluble
18 S H -1.3263 Soluble
19 M H -1.3258 Soluble
20 R H -2.3009 Soluble
21 L H 0.0000 Buried
22 S H -0.4664 Soluble
23 C H 0.0000 Buried
24 A H -0.1149 Soluble
25 G H -0.4897 Soluble
26 S H -0.5416 Soluble
27 G H -0.6709 Soluble
28 F H -0.5715 Soluble
29 T H -0.6332 Soluble
30 F H 0.0000 Buried
35 T H -1.1730 Soluble
36 D H -1.5826 Soluble
37 F H 0.0000 Buried
38 Y H -0.8238 Soluble
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 I H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7339 Soluble
45 P H -1.0538 Soluble
46 A H -0.7127 Soluble
47 G H -1.2993 Soluble
48 K H -2.0253 Soluble
49 A H -1.0405 Soluble
50 P H 0.0000 Buried
51 E H -0.7257 Soluble
52 W H 0.0000 Buried
53 L H 0.0000 Buried
54 G H 0.0000 Buried
55 F H 0.0000 Buried
56 I H 0.0000 Buried
57 R H -1.6902 Soluble
58 D H -1.9632 Soluble
59 K H -2.4875 Soluble
60 A H -1.7293 Soluble
61 K H -2.1474 Soluble
62 G H -1.6538 Soluble
63 Y H -1.1119 Soluble
64 T H -0.9817 Soluble
65 T H -0.8197 Soluble
66 E H -1.6392 Soluble
67 Y H -1.2891 Soluble
68 N H 0.0000 Buried
69 P H -1.5052 Soluble
70 S H -0.8562 Soluble
71 V H 0.0000 Buried
72 K H -2.1531 Soluble
74 G H -1.4145 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -1.0125 Soluble
78 I H 0.0000 Buried
79 S H -0.3540 Soluble
80 R H 0.0000 Buried
81 D H -1.2353 Soluble
82 N H -1.5228 Soluble
83 T H -1.1452 Soluble
84 Q H -1.6898 Soluble
85 N H -1.2506 Soluble
86 M H -0.5579 Soluble
87 L H 0.0000 Buried
88 Y H -0.6225 Soluble
89 L H 0.0000 Buried
90 Q H -1.5423 Soluble
91 M H 0.0000 Buried
92 N H -1.3157 Soluble
93 T H -1.0911 Soluble
94 L H 0.0000 Buried
95 R H -2.2228 Soluble
96 A H -1.7198 Soluble
97 E H -2.2019 Soluble
98 D H 0.0000 Buried
99 T H -0.7607 Soluble
100 A H 0.0000 Buried
101 T H -0.0219 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 E H -0.3954 Soluble
108 G H -0.8967 Soluble
109 H H -1.1803 Soluble
110 T H -0.7159 Soluble
112 A H -0.4742 Soluble
113 A H -0.4321 Soluble
114 P H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.7850 Soluble
117 Y H -0.4769 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.4479 Soluble
121 G H -0.6236 Soluble
122 V H 0.0496 Aggregation-prone
123 M H 0.2391 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1659 Soluble
128 S H -0.9784 Soluble
129 A H -0.8392 Soluble
130 Q H -1.3150 Soluble
131 T H -0.5926 Soluble
132 T H -0.3009 Soluble
133 A H -0.3927 Soluble
134 P H -0.2380 Soluble
135 S H -0.2104 Soluble
136 V H 0.0000 Buried
137 Y H 0.0000 Buried
138 P H -0.7205 Soluble
139 L H 0.0000 Buried
140 A H 0.1231 Aggregation-prone
141 P H -0.4734 Soluble
142 G H -1.0809 Soluble
143 C H -1.7612 Soluble
144 G H -1.8124 Soluble
145 D H -1.9759 Soluble
146 T H -0.9297 Soluble
147 T H -0.6733 Soluble
148 S H -0.5011 Soluble
149 S H -0.3822 Soluble
150 T H -0.2751 Soluble
151 V H 0.0000 Buried
152 T H -0.0948 Soluble
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.2662 Soluble
159 G H 0.0791 Aggregation-prone
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.3686 Soluble
164 P H -0.3531 Soluble
165 V H -0.2002 Soluble
166 T H -0.2347 Soluble
167 V H -0.1653 Soluble
168 T H -0.2925 Soluble
169 W H 0.0000 Buried
170 N H -0.8588 Soluble
171 S H -0.6477 Soluble
172 G H -0.3988 Soluble
173 A H -0.1875 Soluble
174 L H -0.1112 Soluble
175 S H -0.3191 Soluble
176 S H -0.3516 Soluble
177 D H -0.4378 Soluble
178 V H 0.1744 Aggregation-prone
179 H H 0.0000 Buried
180 T H 0.1685 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.1754 Soluble
183 A H 0.3781 Aggregation-prone
184 V H 0.8637 Aggregation-prone
185 L H 1.6452 Aggregation-prone
186 Q H 0.4997 Aggregation-prone
187 S H 0.0752 Aggregation-prone
188 G H 0.0640 Aggregation-prone
189 L H 0.5304 Aggregation-prone
190 Y H 0.7258 Aggregation-prone
191 T H 0.0000 Buried
192 L H 0.0000 Buried
193 T H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 T H -0.1246 Soluble
198 S H 0.0000 Buried
199 S H -0.4901 Soluble
200 T H -0.5683 Soluble
201 W H -0.3887 Soluble
202 P H -0.5877 Soluble
203 S H -0.9255 Soluble
204 Q H -1.3991 Soluble
205 T H -0.9471 Soluble
206 V H 0.0000 Buried
207 T H 0.0000 Buried
208 C H 0.0000 Buried
209 N H -1.4796 Soluble
210 V H 0.0000 Buried
211 A H -0.9258 Soluble
212 H H 0.0000 Buried
213 P H -0.6818 Soluble
214 A H -0.2805 Soluble
215 S H -0.4924 Soluble
216 S H -0.7465 Soluble
217 T H -0.9092 Soluble
218 K H -2.0422 Soluble
219 V H -1.6581 Soluble
220 D H -2.6168 Soluble
221 K H -2.0609 Soluble
222 K H -1.9932 Soluble
223 V H -0.3389 Soluble
1 D G -2.8010 Soluble
2 I G 0.0000 Buried
3 K G -2.6161 Soluble
4 M G 0.0000 Buried
5 T G -1.1979 Soluble
6 Q G 0.0000 Buried
7 S G -0.7088 Soluble
8 P G -0.4203 Soluble
9 S G -0.4095 Soluble
10 F G 0.0170 Aggregation-prone
11 L G -0.1378 Soluble
12 S G -0.4577 Soluble
13 A G -1.0026 Soluble
14 S G -1.0241 Soluble
15 V G -0.2707 Soluble
16 G G -0.8530 Soluble
17 D G -1.9225 Soluble
18 R G -2.4512 Soluble
19 V G 0.0000 Buried
20 T G -0.7441 Soluble
21 L G 0.0000 Buried
22 N G -1.2274 Soluble
23 C G 0.0000 Buried
24 K G -2.4989 Soluble
25 A G -2.2463 Soluble
26 S G -2.2008 Soluble
27 Q G -2.8144 Soluble
28 N G -2.6840 Soluble
29 I G 0.0000 Buried
36 D G -2.5978 Soluble
37 K G -2.4595 Soluble
38 Y G -0.7999 Soluble
39 L G 0.0000 Buried
40 N G 0.0000 Buried
41 W G 0.0000 Buried
42 Y G 0.0000 Buried
43 Q G 0.0000 Buried
44 Q G -1.0889 Soluble
45 K G -1.0427 Soluble
46 L G 0.3929 Aggregation-prone
47 G G -0.8212 Soluble
48 E G -2.0675 Soluble
49 S G -1.5684 Soluble
50 P G 0.0000 Buried
51 K G -1.9428 Soluble
52 L G 0.0000 Buried
53 L G 0.0000 Buried
54 I G 0.0000 Buried
55 Y G -0.6627 Soluble
56 N G -1.2431 Soluble
57 T G 0.0000 Buried
65 N G -1.9131 Soluble
66 N G -1.1973 Soluble
67 L G -0.3357 Soluble
68 Q G -0.5014 Soluble
69 T G -0.2969 Soluble
70 G G -0.3885 Soluble
71 I G -0.0663 Soluble
72 P G -0.2776 Soluble
74 S G -0.4177 Soluble
75 R G -0.8425 Soluble
76 F G 0.0000 Buried
77 S G -0.7589 Soluble
78 G G -0.9710 Soluble
79 S G -1.3901 Soluble
80 G G -1.6263 Soluble
83 S G -1.9649 Soluble
84 G G -2.2412 Soluble
85 T G -2.2722 Soluble
86 D G -2.3425 Soluble
87 F G 0.0000 Buried
88 T G -1.0255 Soluble
89 L G 0.0000 Buried
90 T G -0.6990 Soluble
91 I G 0.0000 Buried
92 S G -1.5067 Soluble
93 S G -1.3134 Soluble
94 L G 0.0000 Buried
95 Q G -0.8758 Soluble
96 P G -0.8797 Soluble
97 E G -1.8535 Soluble
98 D G 0.0000 Buried
99 V G -0.2931 Soluble
100 A G 0.0000 Buried
101 T G -0.2966 Soluble
102 Y G 0.0000 Buried
103 F G 0.0000 Buried
104 C G 0.0000 Buried
105 L G 0.0000 Buried
106 Q G 0.0000 Buried
107 H G -0.4060 Soluble
108 I G -0.2641 Soluble
109 S G -0.9456 Soluble
114 R G -2.3202 Soluble
115 P G -1.8122 Soluble
116 R G -1.2846 Soluble
117 T G -1.0819 Soluble
118 F G -0.2812 Soluble
119 G G 0.0000 Buried
120 T G -0.6317 Soluble
121 G G 0.0000 Buried
122 T G 0.0000 Buried
123 K G -0.9400 Soluble
124 L G 0.0000 Buried
125 E G 0.0000 Buried
126 L G 0.0000 Buried
127 K G -1.9594 Soluble
128 R G -1.6978 Soluble
129 A G -1.2097 Soluble
130 N G -1.8140 Soluble
131 A G -0.9292 Soluble
132 A G -0.3581 Soluble
133 P G -0.3038 Soluble
134 T G -0.1903 Soluble
135 V G 0.0000 Buried
136 S G -0.4599 Soluble
137 I G 0.0000 Buried
138 F G 0.0000 Buried
139 P G -0.5345 Soluble
140 P G -0.4759 Soluble
141 S G -0.6928 Soluble
142 T G -0.9339 Soluble
143 E G -1.8314 Soluble
144 Q G 0.0000 Buried
145 L G -0.7303 Soluble
146 A G -0.7257 Soluble
147 T G -0.6663 Soluble
148 G G -0.9529 Soluble
149 G G -0.8465 Soluble
150 A G 0.0000 Buried
151 S G 0.0000 Buried
152 V G 0.0000 Buried
153 V G 0.0000 Buried
154 C G 0.0000 Buried
155 L G 0.0000 Buried
156 M G 0.0000 Buried
157 N G 0.0000 Buried
158 K G -1.2093 Soluble
159 F G 0.0000 Buried
160 Y G 0.0000 Buried
161 P G -2.3118 Soluble
162 R G -2.7976 Soluble
163 D G -2.4497 Soluble
164 I G -1.2338 Soluble
165 S G -0.7726 Soluble
166 V G -0.1642 Soluble
167 K G -1.5419 Soluble
168 W G 0.0000 Buried
169 K G -2.4765 Soluble
170 I G 0.0000 Buried
171 D G -2.0967 Soluble
172 G G -1.7583 Soluble
173 T G -2.1752 Soluble
174 E G -3.3783 Soluble
175 R G -3.3025 Soluble
176 N G -2.4905 Soluble
177 G G -1.0843 Soluble
178 V G -0.7147 Soluble
179 L G -0.1152 Soluble
180 N G -0.5545 Soluble
181 S G -0.0988 Soluble
182 V G 0.2030 Aggregation-prone
183 T G -0.4968 Soluble
184 D G -1.0648 Soluble
185 Q G -1.2077 Soluble
186 D G -1.2244 Soluble
187 S G -0.9842 Soluble
188 A G -0.5406 Soluble
189 D G -0.8759 Soluble
190 S G -1.2692 Soluble
191 T G 0.0000 Buried
192 Y G 0.0000 Buried
193 S G 0.0000 Buried
194 M G 0.0000 Buried
195 S G 0.0000 Buried
196 S G 0.0000 Buried
197 T G 0.0000 Buried
198 L G 0.0000 Buried
199 S G -0.2034 Soluble
200 L G -0.5754 Soluble
201 T G -1.0268 Soluble
202 K G -1.4687 Soluble
203 A G -1.4324 Soluble
204 D G -2.2660 Soluble
205 Y G 0.0000 Buried
206 Q G -1.8188 Soluble
207 S G -1.4826 Soluble
208 H G -1.7061 Soluble
209 N G -1.8878 Soluble
210 L G -1.1909 Soluble
211 Y G 0.0000 Buried
212 T G 0.0000 Buried
213 C G 0.0000 Buried
214 Q G -0.4676 Soluble
215 V G 0.0000 Buried
216 V G 0.0328 Aggregation-prone
217 H G 0.0000 Buried
218 K G -2.0507 Soluble
219 T G -1.2381 Soluble
220 S G -0.7548 Soluble
221 S G -0.3312 Soluble
222 S G -0.3408 Soluble
223 P G 0.1327 Aggregation-prone
224 V G 0.6220 Aggregation-prone
225 V G 0.7210 Aggregation-prone
226 A G -0.1594 Soluble
227 K G -1.1548 Soluble
228 N G -1.5415 Soluble
229 F G 0.0000 Buried
230 N G -1.6613 Soluble
231 R G -2.1759 Soluble
232 N G -2.4834 Soluble
233 E G -2.6834 Soluble
234 C G -1.2240 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6379 1.9325 CSV PDB
4.5 -0.6798 1.9325 CSV PDB
5.0 -0.7278 1.9325 CSV PDB
5.5 -0.7735 1.9325 CSV PDB
6.0 -0.8067 1.9325 CSV PDB
6.5 -0.8188 1.9325 CSV PDB
7.0 -0.8104 1.9325 CSV PDB
7.5 -0.7894 1.9325 CSV PDB
8.0 -0.7614 1.9325 CSV PDB
8.5 -0.7276 1.9325 CSV PDB
9.0 -0.6864 1.9324 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH