Tomuzotuximab / 5icy

A4DAB272_5icy_D_C | Source PDB: 5icy | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6487
Max Score
2.0395
Min Score
-3.6336
Total Score
-274.39
Score CDR
-0.2437
Score non-CDR
-0.6981

Calculation Parameters

Chain Sequence(s) VQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6336
Maximum Score 2.0395
Average Score -0.6487
Total Score -274.3874

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V D 0.9874 Aggregation-prone
3 Q D -0.7111 Soluble
4 L D 0.0000 Buried
5 K D -2.3395 Soluble
6 Q D 0.0000 Buried
7 S D -0.8645 Soluble
8 G D -1.1242 Soluble
9 P D -0.4202 Soluble
11 G D -0.3400 Soluble
12 L D -0.0651 Soluble
13 V D -0.7659 Soluble
14 Q D -1.6520 Soluble
15 P D -1.4667 Soluble
16 S D -1.4483 Soluble
17 Q D -1.6948 Soluble
18 S D -1.4405 Soluble
19 L D 0.0000 Buried
20 S D -0.5670 Soluble
21 I D 0.0000 Buried
22 T D -0.4891 Soluble
23 C D 0.0000 Buried
24 T D -1.1630 Soluble
25 V D 0.0000 Buried
26 S D -0.6630 Soluble
27 G D -0.0367 Soluble
28 F D -0.0507 Soluble
29 S D -0.7663 Soluble
30 L D 0.0000 Buried
35 T D -0.9194 Soluble
36 N D -0.8640 Soluble
37 Y D 0.4487 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4834 Soluble
45 S D -1.0078 Soluble
46 P D -1.1584 Soluble
47 G D -1.7348 Soluble
48 K D -2.2794 Soluble
49 G D -1.1797 Soluble
50 L D 0.0000 Buried
51 E D -0.6015 Soluble
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.4956 Soluble
58 S D -0.6634 Soluble
59 G D -1.2043 Soluble
63 G D -1.4978 Soluble
64 N D -1.8117 Soluble
65 T D -1.2614 Soluble
66 D D -0.9870 Soluble
67 Y D -0.3970 Soluble
68 N D -0.2013 Soluble
69 T D -0.2478 Soluble
70 P D -0.3937 Soluble
71 F D 0.0000 Buried
72 T D -0.4618 Soluble
74 S D -0.5172 Soluble
75 R D -0.8867 Soluble
76 L D 0.0000 Buried
77 S D -0.9316 Soluble
78 I D 0.0000 Buried
79 N D -1.6967 Soluble
80 K D -1.4631 Soluble
81 D D -1.8154 Soluble
82 N D -2.2637 Soluble
83 S D -1.7196 Soluble
84 K D -2.3948 Soluble
85 S D -1.6360 Soluble
86 Q D -1.5493 Soluble
87 V D 0.0000 Buried
88 F D -0.1462 Soluble
89 F D 0.0000 Buried
90 K D -1.4085 Soluble
91 M D 0.0000 Buried
92 N D -1.4563 Soluble
93 S D -1.3323 Soluble
94 L D 0.0000 Buried
95 Q D -2.0299 Soluble
96 S D -1.5209 Soluble
97 N D -2.0423 Soluble
98 D D 0.0000 Buried
99 T D -0.5835 Soluble
100 A D 0.0000 Buried
101 I D 0.5703 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.1118 Aggregation-prone
109 T D 1.2052 Aggregation-prone
110 Y D 1.8922 Aggregation-prone
111 Y D 1.9857 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.6233 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.4302 Aggregation-prone
117 Y D 0.4912 Aggregation-prone
118 W D -0.3352 Soluble
119 G D 0.0000 Buried
120 Q D -1.8160 Soluble
121 G D -0.8952 Soluble
122 T D 0.0000 Buried
123 L D 0.2622 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3105 Soluble
126 V D 0.0000 Buried
127 S D -0.5780 Soluble
128 A D -0.3888 Soluble
129 A D -0.3119 Soluble
130 S D -0.5473 Soluble
131 T D -0.7189 Soluble
132 K D -1.2405 Soluble
133 G D -1.3452 Soluble
134 P D 0.0000 Buried
135 S D -0.2243 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.2121 Soluble
139 L D 0.0000 Buried
140 A D -1.0742 Soluble
141 G D -0.6396 Soluble
142 T D -0.4798 Soluble
143 A D -0.2230 Soluble
144 A D 0.0000 Buried
145 L D 0.0000 Buried
146 G D 0.0000 Buried
147 C D 0.0000 Buried
148 L D 0.0000 Buried
149 V D 0.0000 Buried
150 K D 0.0000 Buried
151 D D -0.3432 Soluble
152 Y D 0.0000 Buried
153 F D 0.0000 Buried
154 P D 0.0000 Buried
155 E D -0.2031 Soluble
156 P D -0.4448 Soluble
157 V D -0.5082 Soluble
158 T D -0.4942 Soluble
159 V D -0.2178 Soluble
160 S D -0.4061 Soluble
161 W D 0.0000 Buried
162 N D -0.7575 Soluble
163 S D -0.6528 Soluble
164 G D -0.4634 Soluble
165 A D -0.1908 Soluble
166 L D 0.0455 Aggregation-prone
167 T D -0.1371 Soluble
168 S D -0.1716 Soluble
169 G D -0.2131 Soluble
170 V D 0.1573 Aggregation-prone
171 H D -0.2392 Soluble
172 T D 0.0072 Aggregation-prone
173 F D 0.0000 Buried
174 P D -0.2872 Soluble
175 A D 0.2075 Aggregation-prone
176 V D 0.4431 Aggregation-prone
177 L D 1.2086 Aggregation-prone
178 Q D 0.2929 Aggregation-prone
179 S D -0.0568 Soluble
180 S D -0.1963 Soluble
181 G D 0.0648 Aggregation-prone
182 L D 0.2037 Aggregation-prone
183 Y D 0.5091 Aggregation-prone
184 S D 0.0000 Buried
185 L D 0.0000 Buried
186 S D 0.0000 Buried
187 S D 0.0000 Buried
188 V D 0.0000 Buried
189 V D 0.0000 Buried
190 T D -0.1305 Soluble
191 V D 0.0000 Buried
192 P D -0.4832 Soluble
193 S D -0.5149 Soluble
194 S D -0.5805 Soluble
195 S D -0.5379 Soluble
196 L D -0.7432 Soluble
197 G D -0.9194 Soluble
198 T D -0.6583 Soluble
199 Q D -1.0414 Soluble
200 T D -1.0391 Soluble
201 Y D 0.0000 Buried
202 I D -1.3351 Soluble
203 C D 0.0000 Buried
204 N D -1.5123 Soluble
205 V D 0.0000 Buried
206 N D -1.8783 Soluble
207 H D 0.0000 Buried
208 K D -2.3893 Soluble
209 P D -1.3893 Soluble
210 S D -1.6893 Soluble
211 N D -2.4571 Soluble
212 T D -1.9540 Soluble
213 K D -2.6315 Soluble
214 V D -1.5560 Soluble
215 D D -2.5322 Soluble
216 K D -2.1698 Soluble
217 R D -2.5625 Soluble
218 V D 0.0000 Buried
219 E D -2.4957 Soluble
220 P D -1.7210 Soluble
221 K D -2.3399 Soluble
1 D C -1.3208 Soluble
2 I C 0.0000 Buried
3 L C 0.6200 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.4019 Soluble
6 Q C 0.0000 Buried
7 S C 0.1434 Aggregation-prone
8 P C 0.8929 Aggregation-prone
9 V C 2.0395 Aggregation-prone
10 I C 1.5532 Aggregation-prone
11 L C 0.6557 Aggregation-prone
12 S C -0.1049 Soluble
13 V C -0.9675 Soluble
14 S C -1.6844 Soluble
15 P C -1.9174 Soluble
16 G C -2.0796 Soluble
17 E C -2.8892 Soluble
18 R C -3.1070 Soluble
19 V C 0.0000 Buried
20 S C -0.9231 Soluble
21 F C 0.0000 Buried
22 S C -0.8509 Soluble
23 C C 0.0000 Buried
24 R C -2.4562 Soluble
25 A C 0.0000 Buried
26 S C -0.9474 Soluble
27 Q C -1.2459 Soluble
28 S C -1.4419 Soluble
29 I C 0.0000 Buried
36 G C -0.9906 Soluble
37 T C -0.7645 Soluble
38 N C -0.8026 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.3168 Soluble
45 R C -1.9932 Soluble
46 T C -1.5989 Soluble
47 N C -1.8282 Soluble
48 G C -1.5496 Soluble
49 S C -0.9898 Soluble
50 P C 0.0000 Buried
51 R C -0.7375 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7243 Soluble
56 Y C -0.5656 Soluble
57 A C 0.0000 Buried
65 S C -1.2459 Soluble
66 E C -1.9893 Soluble
67 S C -1.1893 Soluble
68 I C -0.5168 Soluble
69 S C -0.3475 Soluble
70 G C -0.4779 Soluble
71 I C -0.2863 Soluble
72 P C -0.3831 Soluble
74 S C -0.5812 Soluble
75 R C -1.0457 Soluble
76 F C 0.0000 Buried
77 S C -0.8958 Soluble
78 G C -0.7609 Soluble
79 S C -0.8519 Soluble
80 G C -1.0992 Soluble
83 S C -0.9255 Soluble
84 G C -1.2595 Soluble
85 T C -1.8599 Soluble
86 D C -2.4895 Soluble
87 F C 0.0000 Buried
88 T C -0.8916 Soluble
89 L C 0.0000 Buried
90 S C -0.8712 Soluble
91 I C 0.0000 Buried
92 N C -2.0583 Soluble
93 S C -2.1160 Soluble
94 V C 0.0000 Buried
95 E C -2.5274 Soluble
96 S C -2.4052 Soluble
97 E C -2.5225 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C 0.0000 Buried
101 D C -0.9647 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6331 Soluble
108 N C -1.2513 Soluble
109 N C -0.9513 Soluble
114 W C 0.1270 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C -0.0608 Soluble
118 F C 0.1732 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0435 Aggregation-prone
121 G C 0.1297 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.1045 Soluble
124 L C 0.0000 Buried
125 E C -0.9106 Soluble
126 L C 0.0000 Buried
127 K C -1.5342 Soluble
128 R C -1.2079 Soluble
129 T C -0.0954 Soluble
130 V C 0.4156 Aggregation-prone
131 A C 0.1532 Aggregation-prone
132 A C -0.0688 Soluble
133 P C 0.0000 Buried
134 S C -0.0854 Soluble
135 V C 0.0368 Aggregation-prone
136 F C 0.2188 Aggregation-prone
137 I C 0.1860 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5231 Soluble
140 P C -0.7302 Soluble
141 S C -1.6781 Soluble
142 D C -2.8635 Soluble
143 E C -2.5056 Soluble
144 Q C 0.0000 Buried
145 L C -2.0975 Soluble
146 K C -2.6164 Soluble
147 S C -1.6503 Soluble
148 G C -1.2487 Soluble
149 T C -0.9902 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.9487 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.6482 Soluble
162 R C -2.6472 Soluble
163 E C -3.0743 Soluble
164 A C -2.1653 Soluble
165 K C -2.2430 Soluble
166 V C -1.2001 Soluble
167 Q C -0.9104 Soluble
168 W C 0.0000 Buried
169 K C -0.7413 Soluble
170 V C 0.0000 Buried
171 D C -1.9809 Soluble
172 N C -1.6092 Soluble
173 A C -0.3853 Soluble
174 L C 0.3418 Aggregation-prone
175 Q C -0.3095 Soluble
176 S C -0.4959 Soluble
177 G C -0.8954 Soluble
178 N C -0.8105 Soluble
179 S C -1.2232 Soluble
180 Q C -1.5031 Soluble
181 E C -2.1812 Soluble
182 S C -1.0328 Soluble
183 V C -0.9437 Soluble
184 T C -0.8126 Soluble
185 E C -1.5056 Soluble
186 Q C 0.0000 Buried
187 D C -1.8960 Soluble
188 S C -2.3975 Soluble
189 K C -2.7858 Soluble
190 D C -2.1131 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.7426 Soluble
199 L C 0.0000 Buried
200 T C -0.3212 Soluble
201 L C -0.6600 Soluble
202 S C -1.0533 Soluble
203 K C -2.0496 Soluble
204 A C -1.9058 Soluble
205 D C -2.5684 Soluble
206 Y C 0.0000 Buried
207 E C -3.4333 Soluble
208 K C -3.6336 Soluble
209 H C -3.2351 Soluble
210 K C -3.2584 Soluble
211 V C -1.6469 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -1.0719 Soluble
216 V C 0.0000 Buried
217 T C -1.1494 Soluble
218 H C 0.0000 Buried
219 Q C -1.6459 Soluble
220 G C -0.3839 Soluble
221 L C -0.1977 Soluble
222 S C -0.4548 Soluble
223 S C -0.4239 Soluble
224 P C -0.4266 Soluble
225 V C -0.1216 Soluble
226 T C -0.6024 Soluble
227 K C -0.7925 Soluble
228 S C -0.6993 Soluble
229 F C -0.8974 Soluble
230 N C -2.0099 Soluble
231 R C -2.4411 Soluble
232 G C -1.5114 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6697 2.6266 CSV PDB
4.5 -0.7069 2.5623 CSV PDB
5.0 -0.7504 2.4857 CSV PDB
5.5 -0.7916 2.404 CSV PDB
6.0 -0.8205 2.3206 CSV PDB
6.5 -0.8289 2.2367 CSV PDB
7.0 -0.8168 2.1985 CSV PDB
7.5 -0.7915 2.1983 CSV PDB
8.0 -0.7589 2.1977 CSV PDB
8.5 -0.7209 2.2242 CSV PDB
9.0 -0.6777 2.2702 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH