Vislarafusp / 6my5

A4DAB295_6my5_H_L | Source PDB: 6my5 | Chains: H, L | Done
93.08
Yes
10 Å
Yes
Avg A4D Score
-0.6873
Max Score
1.8411
Min Score
-3.3953
Total Score
-302.4
Score CDR
-0.3826
Score non-CDR
-0.7272

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTWIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGFMFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIQMTQSPSSLSASVGDRVTITCRASQDIPRRISGYVAWYQQKPGKAPKLLIYWGSYLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3953
Maximum Score 1.8411
Average Score -0.6873
Total Score -302.401

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0869 Soluble
2 V H -1.1892 Soluble
3 Q H -1.1281 Soluble
4 L H 0.0000 Buried
5 V H 0.3575 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5336 Soluble
8 G H -0.9049 Soluble
9 G H -0.4766 Soluble
11 G H -0.2042 Soluble
12 L H -0.0408 Soluble
13 V H -0.6265 Soluble
14 Q H -1.5753 Soluble
15 P H -1.6919 Soluble
16 G H -1.4400 Soluble
17 G H -1.1133 Soluble
18 S H -1.3113 Soluble
19 L H -1.1735 Soluble
20 R H -2.1359 Soluble
21 L H 0.0000 Buried
22 S H -0.4902 Soluble
23 C H 0.0000 Buried
24 A H -0.2509 Soluble
25 A H 0.0000 Buried
26 S H -1.0217 Soluble
27 G H -1.3438 Soluble
28 F H -1.3693 Soluble
29 N H -2.2671 Soluble
30 I H 0.0000 Buried
35 K H -3.0099 Soluble
36 D H -2.5770 Soluble
37 T H 0.0000 Buried
38 W H -0.0366 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8015 Soluble
45 A H -1.1766 Soluble
46 P H -0.9725 Soluble
47 G H -1.4681 Soluble
48 K H -2.3673 Soluble
49 G H -1.6210 Soluble
50 L H 0.0000 Buried
51 E H -1.0441 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.4953 Soluble
58 P H -1.2680 Soluble
59 T H -1.4818 Soluble
62 N H -1.3900 Soluble
63 G H -0.6572 Soluble
64 Y H 0.1464 Aggregation-prone
65 T H -0.1262 Soluble
66 R H -0.8732 Soluble
67 Y H -1.1584 Soluble
68 A H -1.4440 Soluble
69 D H -2.4841 Soluble
70 S H -1.6981 Soluble
71 V H 0.0000 Buried
72 K H -2.6416 Soluble
74 G H -1.7131 Soluble
75 R H -1.3011 Soluble
76 F H 0.0000 Buried
77 T H -0.9311 Soluble
78 I H 0.0000 Buried
79 S H -0.1780 Soluble
80 A H -0.8320 Soluble
81 D H -1.4523 Soluble
82 T H -1.9091 Soluble
83 S H -1.6016 Soluble
84 K H -2.1965 Soluble
85 N H -1.8542 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5514 Soluble
89 L H 0.0000 Buried
90 Q H -1.3053 Soluble
91 M H 0.0000 Buried
92 N H -1.4987 Soluble
93 S H -1.2600 Soluble
94 L H 0.0000 Buried
95 R H -2.3272 Soluble
96 A H -1.7254 Soluble
97 E H -2.2414 Soluble
98 D H 0.0000 Buried
99 T H -0.7158 Soluble
100 A H 0.0000 Buried
101 V H 0.2634 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H 0.0590 Aggregation-prone
107 W H 0.0000 Buried
108 G H 0.0000 Buried
109 G H 0.3544 Aggregation-prone
110 F H 1.8411 Aggregation-prone
111 M H 1.4472 Aggregation-prone
112 F H 1.5990 Aggregation-prone
113 Y H 1.0822 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.0445 Soluble
117 Y H 0.0463 Aggregation-prone
118 W H -0.3202 Soluble
119 G H 0.0000 Buried
120 Q H -1.4253 Soluble
121 G H -0.6513 Soluble
122 T H -0.1085 Soluble
123 L H 0.1713 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4094 Soluble
126 V H 0.0000 Buried
127 S H -0.9348 Soluble
128 S H -0.6874 Soluble
129 A H -0.4365 Soluble
130 S H -0.5353 Soluble
131 T H -0.6978 Soluble
132 K H -1.1583 Soluble
133 G H -1.3254 Soluble
134 P H 0.0000 Buried
135 S H -0.1849 Soluble
136 V H 0.0000 Buried
137 F H -0.5890 Soluble
138 P H -1.0653 Soluble
139 L H 0.0000 Buried
140 A H -1.0085 Soluble
141 P H 0.0000 Buried
142 S H -1.3002 Soluble
143 S H -1.2327 Soluble
144 K H -1.7813 Soluble
145 S H -1.0583 Soluble
146 T H -0.8969 Soluble
147 S H -0.7231 Soluble
148 G H -0.7903 Soluble
149 G H -0.7899 Soluble
150 T H -0.5985 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.4091 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4714 Soluble
164 P H -0.7496 Soluble
165 V H -0.7424 Soluble
166 T H -0.6527 Soluble
167 V H -0.3985 Soluble
168 S H -0.4380 Soluble
169 W H 0.0000 Buried
170 N H -0.7565 Soluble
171 S H -0.6753 Soluble
172 G H -0.5750 Soluble
173 A H -0.2476 Soluble
174 L H -0.0183 Soluble
175 T H -0.1940 Soluble
176 S H -0.2074 Soluble
177 G H -0.2702 Soluble
178 V H 0.1317 Aggregation-prone
179 H H -0.3454 Soluble
180 T H 0.0123 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3462 Soluble
183 A H 0.2138 Aggregation-prone
184 V H 0.5233 Aggregation-prone
185 L H 1.0638 Aggregation-prone
186 Q H 0.2199 Aggregation-prone
187 S H -0.1564 Soluble
188 S H -0.2477 Soluble
189 G H -0.0166 Soluble
190 L H 0.1338 Aggregation-prone
191 Y H 0.4148 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.2425 Soluble
199 V H 0.0000 Buried
200 P H -0.5454 Soluble
201 S H -0.7413 Soluble
202 S H -0.5311 Soluble
203 S H -0.5982 Soluble
204 L H -0.7913 Soluble
205 G H -1.2870 Soluble
206 T H -0.8486 Soluble
207 Q H -1.1257 Soluble
208 T H -1.0122 Soluble
209 Y H 0.0000 Buried
210 I H -1.1647 Soluble
211 C H 0.0000 Buried
212 N H -1.4896 Soluble
213 V H 0.0000 Buried
214 N H -2.2012 Soluble
215 H H 0.0000 Buried
216 K H -2.8240 Soluble
217 P H -1.6235 Soluble
218 S H -1.8732 Soluble
219 N H -2.6388 Soluble
220 T H -2.1245 Soluble
221 K H -2.7382 Soluble
222 V H -1.5611 Soluble
223 D H -2.4508 Soluble
224 K H -1.9802 Soluble
225 K H -2.3306 Soluble
226 V H 0.0000 Buried
227 E H -2.3727 Soluble
228 P H -1.7440 Soluble
229 K H -2.3323 Soluble
230 S H -1.1854 Soluble
1 D L -2.1133 Soluble
2 I L -1.6696 Soluble
3 Q L -1.9270 Soluble
4 M L 0.0000 Buried
5 T L -1.2785 Soluble
6 Q L 0.0000 Buried
7 S L -0.8656 Soluble
8 P L -0.5307 Soluble
9 S L -0.9016 Soluble
10 S L -0.8044 Soluble
11 L L -0.4888 Soluble
12 S L -0.6267 Soluble
13 A L -0.9402 Soluble
14 S L -0.9070 Soluble
15 V L -0.2328 Soluble
16 G L -0.8616 Soluble
17 D L -1.7304 Soluble
18 R L -2.3185 Soluble
19 V L 0.0000 Buried
20 T L -0.6283 Soluble
21 I L 0.0000 Buried
22 T L -0.6642 Soluble
23 C L 0.0000 Buried
24 R L -2.3081 Soluble
25 A L -1.9303 Soluble
26 S L -2.0535 Soluble
27 Q L -2.6286 Soluble
28 D L -2.6473 Soluble
29 I L 0.0000 Buried
30 P L -1.7850 Soluble
31 R L -2.7701 Soluble
34 R L -2.2342 Soluble
35 I L -0.1734 Soluble
36 S L -0.1033 Soluble
37 G L 0.0000 Buried
38 Y L 0.8499 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.2216 Soluble
45 K L -1.6546 Soluble
46 P L -1.4682 Soluble
47 G L -1.7992 Soluble
48 K L -2.5138 Soluble
49 A L -1.5176 Soluble
50 P L 0.0000 Buried
51 K L -1.2184 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.1489 Aggregation-prone
56 W L 1.1003 Aggregation-prone
57 G L 0.3912 Aggregation-prone
65 S L 0.6019 Aggregation-prone
66 Y L 1.6578 Aggregation-prone
67 L L 1.2516 Aggregation-prone
68 Y L 0.5453 Aggregation-prone
69 S L -0.0542 Soluble
70 G L -0.4613 Soluble
71 V L 0.0000 Buried
72 P L -0.3393 Soluble
74 S L -0.4009 Soluble
75 R L -0.7211 Soluble
76 F L 0.0000 Buried
77 S L -0.0217 Soluble
78 G L 0.0383 Aggregation-prone
79 S L -0.4514 Soluble
80 G L -1.0142 Soluble
83 S L -0.8317 Soluble
84 G L -1.6597 Soluble
85 T L -1.5605 Soluble
86 D L -1.4127 Soluble
87 F L -0.9143 Soluble
88 T L -0.5945 Soluble
89 L L 0.0000 Buried
90 T L -0.5917 Soluble
91 I L 0.0000 Buried
92 S L -1.3333 Soluble
93 S L -1.1423 Soluble
94 L L 0.0000 Buried
95 Q L -0.9523 Soluble
96 P L -1.7089 Soluble
97 E L -2.1514 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.9633 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.0000 Buried
108 Y L 0.4260 Aggregation-prone
109 T L -0.1655 Soluble
114 T L -0.1258 Soluble
115 P L -0.3861 Soluble
116 P L 0.0000 Buried
117 T L -0.3718 Soluble
118 F L -0.4434 Soluble
119 G L 0.0000 Buried
120 Q L -1.7448 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.6788 Soluble
124 V L 0.0000 Buried
125 E L -1.2202 Soluble
126 I L 0.0000 Buried
127 K L -1.2209 Soluble
128 R L -0.8107 Soluble
129 T L 0.1939 Aggregation-prone
130 V L 0.9946 Aggregation-prone
131 A L 0.2729 Aggregation-prone
132 A L -0.0164 Soluble
133 P L 0.0000 Buried
134 S L -0.3516 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L -0.0561 Soluble
138 F L 0.0000 Buried
139 P L -0.4029 Soluble
140 P L -0.7443 Soluble
141 S L 0.0000 Buried
142 D L -3.0179 Soluble
143 E L -2.7811 Soluble
144 Q L 0.0000 Buried
145 L L -2.2107 Soluble
146 K L -2.7282 Soluble
147 S L -1.6762 Soluble
148 G L -1.2767 Soluble
149 T L -0.9609 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -1.0085 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5504 Soluble
162 R L -2.9005 Soluble
163 E L -3.1558 Soluble
164 A L -2.3246 Soluble
165 K L -2.4611 Soluble
166 V L -1.1874 Soluble
167 Q L -0.7338 Soluble
168 W L 0.0000 Buried
169 K L -0.7719 Soluble
170 V L 0.0000 Buried
171 D L -1.7502 Soluble
172 N L -1.5279 Soluble
173 A L -0.3191 Soluble
174 L L 0.6011 Aggregation-prone
175 Q L -0.1697 Soluble
176 S L -0.3990 Soluble
177 G L -0.7728 Soluble
178 N L -0.9237 Soluble
179 S L -1.1194 Soluble
180 Q L -1.2999 Soluble
181 E L -1.7951 Soluble
182 S L -0.9692 Soluble
183 V L -1.0210 Soluble
184 T L -1.1877 Soluble
185 E L -2.2754 Soluble
186 Q L 0.0000 Buried
187 D L -2.1526 Soluble
188 S L -2.3574 Soluble
189 K L -2.6919 Soluble
190 D L -2.0385 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6385 Soluble
199 L L 0.0000 Buried
200 T L -0.4539 Soluble
201 L L -0.6177 Soluble
202 S L -1.1604 Soluble
203 K L -1.9994 Soluble
204 A L -1.8137 Soluble
205 D L -2.4643 Soluble
206 Y L 0.0000 Buried
207 E L -3.2386 Soluble
208 K L -3.3953 Soluble
209 H L -2.5622 Soluble
210 K L -2.1176 Soluble
211 V L -0.6205 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -1.0209 Soluble
216 V L 0.0000 Buried
217 T L -1.2645 Soluble
218 H L 0.0000 Buried
219 Q L -1.5904 Soluble
220 G L -0.2264 Soluble
221 L L -0.1552 Soluble
222 S L -0.4651 Soluble
223 S L -0.4320 Soluble
224 P L -0.6365 Soluble
225 V L -0.1277 Soluble
226 T L -0.5155 Soluble
227 K L -0.6720 Soluble
228 S L -0.4426 Soluble
229 F L -0.4715 Soluble
230 N L -1.2287 Soluble
231 R L -1.8356 Soluble
232 G L -1.6864 Soluble
233 E L -1.9799 Soluble
234 C L -0.8786 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.733 4.8431 CSV PDB
4.5 -0.7761 4.7901 CSV PDB
5.0 -0.8251 4.7348 CSV PDB
5.5 -0.8712 4.6787 CSV PDB
6.0 -0.9034 4.6225 CSV PDB
6.5 -0.9123 4.5665 CSV PDB
7.0 -0.8974 4.5114 CSV PDB
7.5 -0.8663 4.4588 CSV PDB
8.0 -0.8256 4.4129 CSV PDB
8.5 -0.7773 4.38 CSV PDB
9.0 -0.7216 4.3622 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH