Zinlirvimab / 5t3x

A4DAB308_5t3x_H_L | Source PDB: 5t3x | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6227
Max Score
2.2241
Min Score
-3.2085
Total Score
-272.75
Score CDR
-0.2939
Score non-CDR
-0.6769

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSETLSVTCSVSGDSMNNYYWTWIRQSPGKGLEWIGYISDRESATYNPSLNSRVVISRDTSKNQLSLKLNSVTPADTAVYYCATARRGQRIYGVVSFGEFFYYYSMDVWGKGTTVTVSSASTKGPSVFPLAPSSKTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCLSVALGETARISCGRQALGSRAVQWYQHRPGQAPILLIYNNQDRPSGIPERFSGTPDINFGTRATLTISGVEAGDEADYYCHMWDSRSGFSWSFGGATRLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2085
Maximum Score 2.2241
Average Score -0.6227
Total Score -272.7481

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.7532 Soluble
2 V H -1.3370 Soluble
3 Q H -1.9149 Soluble
4 L H 0.0000 Buried
5 Q H -1.5807 Soluble
6 E H 0.0000 Buried
7 S H -0.5757 Soluble
8 G H -0.2442 Soluble
9 P H -0.2049 Soluble
11 G H 0.0000 Buried
12 L H -0.2987 Soluble
13 V H -0.9864 Soluble
14 K H -2.1310 Soluble
15 P H -1.6504 Soluble
16 S H -1.6227 Soluble
17 E H -2.2234 Soluble
18 T H -1.7462 Soluble
19 L H 0.0000 Buried
20 S H -0.8554 Soluble
21 V H 0.0000 Buried
22 T H -0.5345 Soluble
23 C H 0.0000 Buried
24 S H -1.2873 Soluble
25 V H 0.0000 Buried
26 S H -1.6970 Soluble
27 G H -1.7362 Soluble
28 D H -1.9614 Soluble
29 S H -1.3622 Soluble
30 M H 0.0000 Buried
35 N H -2.4560 Soluble
36 N H -1.7432 Soluble
37 Y H -0.5908 Soluble
38 Y H -0.8508 Soluble
39 W H 0.0000 Buried
40 T H 0.0000 Buried
41 W H 0.0000 Buried
42 I H -0.2722 Soluble
43 R H -0.6642 Soluble
44 Q H -1.2125 Soluble
45 S H -1.2018 Soluble
46 P H -1.0882 Soluble
47 G H -1.5000 Soluble
48 K H -2.3156 Soluble
49 G H -1.6477 Soluble
50 L H -1.1885 Soluble
51 E H -2.0249 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 Y H 0.0560 Aggregation-prone
56 I H 0.0000 Buried
57 S H -1.7794 Soluble
58 D H -2.4617 Soluble
59 R H -3.2008 Soluble
63 E H -3.2085 Soluble
64 S H -1.7134 Soluble
65 A H -0.5600 Soluble
66 T H 0.0813 Aggregation-prone
67 Y H 0.2207 Aggregation-prone
68 N H 0.0000 Buried
69 P H -0.6564 Soluble
70 S H -0.8254 Soluble
71 L H -0.7862 Soluble
72 N H -1.6690 Soluble
74 S H -1.0717 Soluble
75 R H -1.6798 Soluble
76 V H 0.0000 Buried
77 V H 0.6717 Aggregation-prone
78 I H 0.0000 Buried
79 S H -0.5926 Soluble
80 R H -1.3333 Soluble
81 D H -1.7098 Soluble
82 T H -1.5803 Soluble
83 S H -1.5166 Soluble
84 K H -2.4625 Soluble
85 N H -2.1830 Soluble
86 Q H -1.7329 Soluble
87 L H 0.0000 Buried
88 S H -0.5032 Soluble
89 L H 0.0000 Buried
90 K H -1.1576 Soluble
91 L H -1.2630 Soluble
92 N H -2.0978 Soluble
93 S H -1.6687 Soluble
94 V H 0.0000 Buried
95 T H -0.9869 Soluble
96 P H -0.6987 Soluble
97 A H -0.3318 Soluble
98 D H -0.5617 Soluble
99 T H -0.2583 Soluble
100 A H 0.0000 Buried
101 V H 0.1537 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H -0.4100 Soluble
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 T H 0.0000 Buried
107 A H 0.0000 Buried
108 R H -1.6723 Soluble
109 R H -1.7293 Soluble
110 G H -1.0073 Soluble
111 Q H -0.3644 Soluble
111A R H 0.2363 Aggregation-prone
111B I H 1.5241 Aggregation-prone
111C Y H 1.4715 Aggregation-prone
111D G H 1.4242 Aggregation-prone
111E V H 2.1857 Aggregation-prone
111F V H 2.2241 Aggregation-prone
112G S H 1.3813 Aggregation-prone
112F F H 2.0619 Aggregation-prone
112E G H 0.9929 Aggregation-prone
112D E H 0.8016 Aggregation-prone
112C F H 1.2905 Aggregation-prone
112B F H 0.5484 Aggregation-prone
112A Y H 0.5604 Aggregation-prone
112 Y H 0.0000 Buried
113 Y H 0.0000 Buried
114 S H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -1.0532 Soluble
117 V H -0.2314 Soluble
118 W H -0.3926 Soluble
119 G H -1.2739 Soluble
120 K H -2.3146 Soluble
121 G H -1.2210 Soluble
122 T H -0.4726 Soluble
123 T H 0.0211 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.3095 Soluble
126 V H 0.0000 Buried
127 S H -0.7269 Soluble
128 S H -0.7978 Soluble
129 A H -0.5211 Soluble
130 S H -0.6981 Soluble
131 T H -0.7534 Soluble
132 K H -1.1793 Soluble
133 G H -1.3989 Soluble
134 P H 0.0000 Buried
135 S H -0.3277 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H 0.0000 Buried
139 L H 0.0000 Buried
140 A H 0.0000 Buried
141 P H 0.0000 Buried
142 S H -0.8295 Soluble
143 S H -1.2303 Soluble
144 K H -2.0105 Soluble
145 T H -0.2011 Soluble
146 A H -0.2807 Soluble
147 A H 0.0142 Aggregation-prone
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.5432 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.5012 Soluble
159 P H -0.4218 Soluble
160 V H -0.5620 Soluble
161 T H -0.3734 Soluble
162 V H -0.1872 Soluble
163 S H -0.2157 Soluble
164 W H 0.0000 Buried
165 N H -0.7195 Soluble
166 S H -0.6058 Soluble
167 G H -0.4802 Soluble
168 A H -0.1417 Soluble
169 L H 0.1086 Aggregation-prone
170 T H -0.0052 Soluble
171 S H -0.1199 Soluble
172 G H -0.0067 Soluble
173 V H 0.4143 Aggregation-prone
174 H H -0.3694 Soluble
175 T H -0.1671 Soluble
176 F H 0.0000 Buried
177 P H 0.0550 Aggregation-prone
178 A H 0.2987 Aggregation-prone
179 V H 0.0000 Buried
180 L H 1.3103 Aggregation-prone
181 Q H 0.3007 Aggregation-prone
182 S H -0.1052 Soluble
183 S H -0.1879 Soluble
184 G H 0.0729 Aggregation-prone
185 L H 0.1147 Aggregation-prone
186 Y H 0.3677 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.2127 Aggregation-prone
192 V H 0.0000 Buried
193 T H 0.0272 Aggregation-prone
194 V H 0.1076 Aggregation-prone
195 P H -0.2403 Soluble
196 S H -0.2507 Soluble
197 S H -0.2714 Soluble
198 S H -0.1411 Soluble
199 L H -0.0096 Soluble
200 G H -0.7298 Soluble
201 T H -0.4027 Soluble
202 Q H -0.7975 Soluble
203 T H -0.9427 Soluble
204 Y H 0.0000 Buried
205 I H -1.3013 Soluble
206 C H 0.0000 Buried
207 N H -1.6448 Soluble
208 V H 0.0000 Buried
209 N H -1.7406 Soluble
210 H H 0.0000 Buried
211 K H -1.7752 Soluble
212 P H -1.0102 Soluble
213 S H -1.5392 Soluble
214 N H -2.1532 Soluble
215 T H -2.0523 Soluble
216 K H -2.7054 Soluble
217 V H -2.0216 Soluble
218 D H -2.8136 Soluble
219 K H -2.6160 Soluble
220 R H -2.8133 Soluble
221 V H 0.0000 Buried
222 E H -2.4818 Soluble
223 P H -1.1025 Soluble
224 K H -0.7129 Soluble
225 S H -0.2787 Soluble
226 C H 0.3401 Aggregation-prone
11 L L 0.9979 Aggregation-prone
12 S L 0.5260 Aggregation-prone
13 V L -0.1013 Soluble
14 A L -0.3646 Soluble
15 L L -0.7027 Soluble
16 G L -1.6391 Soluble
17 E L -2.1704 Soluble
18 T L -1.6847 Soluble
19 A L 0.0000 Buried
20 R L -1.9023 Soluble
21 I L 0.0000 Buried
22 S L -1.3451 Soluble
23 C L 0.0000 Buried
24 G L -1.4118 Soluble
25 R L -1.5604 Soluble
26 Q L -1.6390 Soluble
27 A L 0.0000 Buried
28 L L 0.0283 Aggregation-prone
29 G L -0.1243 Soluble
36 S L 0.0000 Buried
37 R L 0.0000 Buried
38 A L 0.0000 Buried
39 V L 0.0000 Buried
40 Q L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.2784 Soluble
44 H L -0.8728 Soluble
45 R L -1.4456 Soluble
46 P L -1.4027 Soluble
47 G L -1.4904 Soluble
48 Q L -1.9249 Soluble
49 A L -1.0456 Soluble
50 P L -0.2323 Soluble
51 I L 0.4696 Aggregation-prone
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.7538 Soluble
56 N L -1.0328 Soluble
57 N L 0.0000 Buried
65 Q L -1.3741 Soluble
66 D L -1.6047 Soluble
67 R L -1.7093 Soluble
68 P L -0.9404 Soluble
69 S L -0.5784 Soluble
70 G L -0.7995 Soluble
71 I L -0.6640 Soluble
72 P L -1.3361 Soluble
74 E L -2.2839 Soluble
75 R L -1.5425 Soluble
76 F L 0.0000 Buried
77 S L -1.2826 Soluble
78 G L 0.0000 Buried
79 T L 0.0000 Buried
80 P L -0.4542 Soluble
81 D L 0.0603 Aggregation-prone
81A I L 1.4139 Aggregation-prone
82 N L 0.2365 Aggregation-prone
83 F L 0.6171 Aggregation-prone
84 G L -0.2189 Soluble
85 T L -0.8388 Soluble
86 R L -2.0882 Soluble
87 A L 0.0000 Buried
88 T L 0.0000 Buried
89 L L 0.0000 Buried
90 T L -1.2001 Soluble
91 I L 0.0000 Buried
92 S L -1.7451 Soluble
93 G L -1.5203 Soluble
94 V L 0.0000 Buried
95 E L -2.1623 Soluble
96 A L -0.9641 Soluble
97 G L -1.1933 Soluble
98 D L 0.0000 Buried
99 E L -0.8999 Soluble
100 A L 0.0000 Buried
101 D L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 M L 0.0000 Buried
107 W L 0.0000 Buried
108 D L 0.0000 Buried
109 S L -0.4131 Soluble
110 R L -1.5837 Soluble
112 S L -0.7478 Soluble
113 G L -0.1726 Soluble
114 F L 0.6626 Aggregation-prone
115 S L 0.0000 Buried
116 W L -0.0060 Soluble
117 S L -0.4788 Soluble
118 F L 0.0000 Buried
119 G L -0.7965 Soluble
120 G L -0.9610 Soluble
121 A L -0.8137 Soluble
122 T L -0.7747 Soluble
123 R L -1.6746 Soluble
124 L L 0.0000 Buried
125 T L -0.0271 Soluble
126 V L 0.0000 Buried
127 L L 0.5456 Aggregation-prone
128 G L -0.3482 Soluble
129 Q L -0.6878 Soluble
130 P L -1.1136 Soluble
131 K L -2.2012 Soluble
132 A L -1.2637 Soluble
133 A L -0.6946 Soluble
134 P L 0.0000 Buried
135 S L -0.4621 Soluble
136 V L 0.0000 Buried
137 T L -0.3855 Soluble
138 L L 0.0000 Buried
139 F L 0.0365 Aggregation-prone
140 P L -0.1051 Soluble
141 P L 0.0000 Buried
142 S L 0.0000 Buried
143 S L -1.1638 Soluble
144 E L -1.6688 Soluble
145 E L 0.0000 Buried
146 L L -1.3626 Soluble
147 Q L -1.9361 Soluble
148 A L -1.5788 Soluble
149 N L -2.2546 Soluble
150 K L -1.8630 Soluble
151 A L 0.0000 Buried
152 T L -0.1215 Soluble
153 L L 0.0000 Buried
154 V L 0.0000 Buried
155 C L 0.0000 Buried
156 L L 0.0000 Buried
157 I L 0.0000 Buried
158 S L 0.0000 Buried
159 D L -1.2837 Soluble
160 F L 0.0000 Buried
161 Y L -1.3053 Soluble
162 P L -0.9391 Soluble
163 G L -0.6637 Soluble
164 A L -0.2379 Soluble
165 V L -0.1956 Soluble
166 T L -0.0500 Soluble
167 V L 0.0000 Buried
168 A L -0.5251 Soluble
169 W L 0.0000 Buried
170 K L -0.8728 Soluble
171 A L 0.0000 Buried
172 D L -1.3520 Soluble
173 S L -0.8668 Soluble
174 S L -0.7188 Soluble
175 P L -0.8750 Soluble
176 V L -0.8429 Soluble
177 K L -1.6360 Soluble
178 A L -0.7189 Soluble
179 G L -0.4808 Soluble
180 V L -0.2938 Soluble
181 E L -0.1549 Soluble
182 T L 0.0492 Aggregation-prone
183 T L 0.0000 Buried
184 T L -0.2703 Soluble
185 P L -0.4888 Soluble
186 S L -1.0370 Soluble
187 K L -2.1752 Soluble
188 Q L -1.6637 Soluble
189 S L -1.2260 Soluble
190 N L -1.4463 Soluble
191 N L -1.4824 Soluble
192 K L -1.6526 Soluble
193 Y L -1.1739 Soluble
194 A L 0.0000 Buried
195 A L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 Y L 0.0000 Buried
199 L L 0.0000 Buried
200 S L -0.4105 Soluble
201 L L -0.6422 Soluble
202 T L -1.5800 Soluble
203 P L -2.1656 Soluble
204 E L -3.0408 Soluble
205 Q L -2.2920 Soluble
206 W L 0.0000 Buried
207 K L -3.0527 Soluble
208 S L -2.4523 Soluble
209 H L -2.4187 Soluble
210 R L -2.5395 Soluble
211 S L -1.7045 Soluble
212 Y L 0.0000 Buried
213 S L 0.0000 Buried
214 C L 0.0000 Buried
215 Q L -0.8830 Soluble
216 V L 0.0000 Buried
217 T L -0.6891 Soluble
218 H L 0.0000 Buried
219 E L -2.2935 Soluble
220 G L -1.4477 Soluble
221 S L -1.0054 Soluble
222 T L -0.7219 Soluble
223 V L -0.3110 Soluble
224 E L -1.3863 Soluble
225 K L -1.3844 Soluble
226 T L -0.7580 Soluble
227 V L -0.7183 Soluble
228 A L -1.4037 Soluble
229 P L -1.4383 Soluble
230 T L -1.4023 Soluble
231 E L -1.8638 Soluble
232 C L -0.8429 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5984 4.3111 CSV PDB
4.5 -0.633 4.2662 CSV PDB
5.0 -0.6759 4.2013 CSV PDB
5.5 -0.7176 4.1273 CSV PDB
6.0 -0.7477 4.0557 CSV PDB
6.5 -0.7577 3.9982 CSV PDB
7.0 -0.7461 3.963 CSV PDB
7.5 -0.7193 3.9471 CSV PDB
8.0 -0.6844 3.9413 CSV PDB
8.5 -0.6451 3.9393 CSV PDB
9.0 -0.6023 3.9387 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH