Anbenitamab / 5tdo

A4DAB016_5tdo_D_C | Source PDB: 5tdo | Chains: D, C | Done
98.68
Yes
10 Å
Yes
Avg A4D Score
-0.7017
Max Score
1.4652
Min Score
-3.6251
Total Score
-298.23
Score CDR
-0.4691
Score non-CDR
-0.7306

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRKAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLESVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQEKPGKAPKLLIYSASFLESGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVKCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6251
Maximum Score 1.4652
Average Score -0.7017
Total Score -298.2289

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.1499 Soluble
2 V D -1.4342 Soluble
3 Q D -1.4731 Soluble
4 L D 0.0000 Buried
5 V D 0.3892 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.4843 Soluble
8 G D -0.9113 Soluble
9 G D -0.3566 Soluble
11 G D 0.1174 Aggregation-prone
12 L D 0.2025 Aggregation-prone
13 V D -0.5177 Soluble
14 Q D -1.5229 Soluble
15 P D -1.6445 Soluble
16 G D -1.3844 Soluble
17 G D -1.0756 Soluble
18 S D -1.2605 Soluble
19 L D -1.1957 Soluble
20 R D -2.2415 Soluble
21 L D 0.0000 Buried
22 S D -0.5279 Soluble
23 C D 0.0000 Buried
24 A D -0.1961 Soluble
25 A D -0.6966 Soluble
26 S D -1.1111 Soluble
27 G D -1.4861 Soluble
28 F D -1.4143 Soluble
29 N D -2.1842 Soluble
30 I D 0.0000 Buried
35 K D -2.5979 Soluble
36 D D -2.8858 Soluble
37 T D 0.0000 Buried
38 Y D -0.4561 Soluble
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 K D -0.8913 Soluble
45 A D -1.2463 Soluble
46 P D -0.9870 Soluble
47 G D -1.4899 Soluble
48 K D -2.4445 Soluble
49 G D -1.6793 Soluble
50 L D 0.0000 Buried
51 E D -1.3733 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 R D -0.5735 Soluble
56 I D 0.0000 Buried
57 Y D -0.2955 Soluble
58 P D -0.9450 Soluble
59 T D -1.0732 Soluble
62 N D -0.7947 Soluble
63 G D -0.2583 Soluble
64 Y D 0.5590 Aggregation-prone
65 T D -0.4113 Soluble
66 R D -1.9607 Soluble
67 Y D -1.7380 Soluble
68 A D -1.8134 Soluble
69 D D -2.6198 Soluble
70 S D -1.6737 Soluble
71 V D 0.0000 Buried
72 K D -2.7834 Soluble
74 G D -1.6659 Soluble
75 R D -1.2483 Soluble
76 F D 0.0000 Buried
77 T D -1.1746 Soluble
78 I D 0.0000 Buried
79 S D -0.1844 Soluble
80 A D -0.8618 Soluble
81 D D -1.4427 Soluble
82 T D -1.8181 Soluble
83 S D -1.5649 Soluble
84 K D -2.1997 Soluble
85 N D -1.7197 Soluble
86 T D -1.0304 Soluble
87 A D 0.0000 Buried
88 Y D -0.6239 Soluble
89 L D 0.0000 Buried
90 Q D -1.5816 Soluble
91 M D 0.0000 Buried
92 N D -1.3072 Soluble
93 S D -1.1680 Soluble
94 L D 0.0000 Buried
95 R D -2.3293 Soluble
96 A D -1.7166 Soluble
97 E D -2.2645 Soluble
98 D D 0.0000 Buried
99 T D -0.6313 Soluble
100 A D 0.0000 Buried
101 V D 0.4178 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
105 S D 0.0000 Buried
106 R D -0.5193 Soluble
107 W D -0.3789 Soluble
108 G D 0.0000 Buried
109 G D -0.9453 Soluble
110 D D -1.7766 Soluble
111 G D 0.0000 Buried
112 F D 0.8144 Aggregation-prone
113 Y D 0.7539 Aggregation-prone
114 A D 0.0000 Buried
115 M D 0.0000 Buried
116 D D 0.0857 Aggregation-prone
117 Y D -0.0735 Soluble
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.6570 Soluble
121 G D 0.0000 Buried
122 T D 0.0980 Aggregation-prone
123 L D 0.6589 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.2626 Soluble
126 V D 0.0000 Buried
127 S D -0.8893 Soluble
128 S D -0.6598 Soluble
129 A D -0.4342 Soluble
130 S D -0.5623 Soluble
131 T D -0.7591 Soluble
132 K D -1.2292 Soluble
133 G D -1.4270 Soluble
134 P D 0.0000 Buried
135 S D -0.4643 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.2277 Soluble
139 L D 0.0000 Buried
140 A D -0.8347 Soluble
141 P D -0.4232 Soluble
142 G D -0.6468 Soluble
143 T D -0.5085 Soluble
144 A D -0.2175 Soluble
145 A D 0.0000 Buried
146 L D 0.0000 Buried
147 G D 0.0000 Buried
148 C D 0.0000 Buried
149 L D 0.0000 Buried
150 V D 0.0000 Buried
151 K D -0.2449 Soluble
152 D D -0.3057 Soluble
153 Y D 0.0000 Buried
154 F D 0.0000 Buried
155 P D 0.0000 Buried
156 E D -0.5199 Soluble
157 P D -0.7903 Soluble
158 V D -0.7613 Soluble
159 T D -0.6547 Soluble
160 V D -0.2755 Soluble
161 S D -0.4589 Soluble
162 W D 0.0000 Buried
163 N D -0.8501 Soluble
164 S D -0.7236 Soluble
165 G D -0.5443 Soluble
166 A D -0.2514 Soluble
167 L D -0.0058 Soluble
168 T D -0.1736 Soluble
169 S D -0.1904 Soluble
170 G D -0.2335 Soluble
171 V D 0.1299 Aggregation-prone
172 H D -0.2911 Soluble
173 T D 0.0258 Aggregation-prone
174 F D 0.0000 Buried
175 P D -0.2540 Soluble
176 A D 0.2382 Aggregation-prone
177 V D 0.5315 Aggregation-prone
178 L D 1.2452 Aggregation-prone
179 Q D 0.2970 Aggregation-prone
180 S D -0.1717 Soluble
181 S D -0.1550 Soluble
182 G D 0.1366 Aggregation-prone
183 L D 0.2080 Aggregation-prone
184 Y D 0.4865 Aggregation-prone
185 S D 0.0000 Buried
186 L D 0.0000 Buried
187 E D 0.0000 Buried
188 S D 0.0000 Buried
189 V D 0.0000 Buried
190 V D 0.0000 Buried
191 T D -0.1700 Soluble
192 V D 0.0000 Buried
193 P D -0.4810 Soluble
194 S D -0.5036 Soluble
195 S D -0.5874 Soluble
196 S D -0.6167 Soluble
197 L D -0.7993 Soluble
198 G D -1.3293 Soluble
199 T D -0.8807 Soluble
200 Q D -1.5952 Soluble
201 T D -1.4030 Soluble
202 Y D 0.0000 Buried
203 I D -1.3539 Soluble
204 C D 0.0000 Buried
205 N D -1.5971 Soluble
206 V D 0.0000 Buried
207 N D -2.1140 Soluble
208 H D 0.0000 Buried
209 K D -2.8157 Soluble
210 P D -1.6242 Soluble
211 S D -1.8850 Soluble
212 N D -2.6366 Soluble
213 T D -2.1447 Soluble
214 K D -2.7764 Soluble
215 V D -1.6770 Soluble
216 D D -2.6052 Soluble
217 K D -2.2485 Soluble
218 K D -2.5955 Soluble
219 V D 0.0000 Buried
220 E D -2.5530 Soluble
221 P D -1.1798 Soluble
2 I C -0.4146 Soluble
3 Q C -1.5665 Soluble
4 M C 0.0000 Buried
5 T C -1.3411 Soluble
6 Q C 0.0000 Buried
7 S C -0.7719 Soluble
8 P C -0.7101 Soluble
9 S C -1.0188 Soluble
10 S C -0.9859 Soluble
11 L C -0.6152 Soluble
12 S C -0.6269 Soluble
13 A C -0.7587 Soluble
14 S C -0.5282 Soluble
15 V C 0.5103 Aggregation-prone
16 G C -0.5932 Soluble
17 D C -1.6233 Soluble
18 R C -2.2121 Soluble
19 V C 0.0000 Buried
20 T C -0.6042 Soluble
21 I C 0.0000 Buried
22 T C -0.7378 Soluble
23 C C 0.0000 Buried
24 R C -2.8221 Soluble
25 A C 0.0000 Buried
26 S C -1.8394 Soluble
27 Q C -2.5489 Soluble
28 D C -2.8035 Soluble
29 V C 0.0000 Buried
36 N C -1.7340 Soluble
37 T C -0.7199 Soluble
38 A C 0.1343 Aggregation-prone
39 V C 0.0000 Buried
40 A C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 E C 0.0000 Buried
45 K C -1.1234 Soluble
46 P C -1.2935 Soluble
47 G C -1.6979 Soluble
48 K C -2.6352 Soluble
49 A C -1.7210 Soluble
50 P C 0.0000 Buried
51 K C -1.3134 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C 0.9797 Aggregation-prone
56 S C 0.5573 Aggregation-prone
57 A C 0.0000 Buried
65 S C 0.3515 Aggregation-prone
66 F C 1.4652 Aggregation-prone
67 L C 0.8079 Aggregation-prone
68 E C 0.0307 Aggregation-prone
69 S C -0.2862 Soluble
70 G C -0.6065 Soluble
71 V C 0.0000 Buried
72 P C -0.4126 Soluble
74 S C -0.4503 Soluble
75 R C -0.6800 Soluble
76 F C 0.0000 Buried
77 S C -0.0319 Soluble
78 G C -0.0373 Soluble
79 S C -0.6072 Soluble
80 R C -1.4980 Soluble
83 S C -1.2693 Soluble
84 G C -1.8094 Soluble
85 T C -2.2439 Soluble
86 D C -2.0166 Soluble
87 F C 0.0000 Buried
88 T C -0.7363 Soluble
89 L C 0.0000 Buried
90 T C -0.5895 Soluble
91 I C 0.0000 Buried
92 S C -1.3241 Soluble
93 S C -1.0164 Soluble
94 L C 0.0000 Buried
95 Q C -0.6308 Soluble
96 P C -1.0694 Soluble
97 E C -1.5675 Soluble
98 D C 0.0000 Buried
99 F C -0.4909 Soluble
100 A C 0.0000 Buried
101 T C 0.0000 Buried
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.5933 Aggregation-prone
108 Y C 0.6949 Aggregation-prone
109 T C 0.6247 Aggregation-prone
114 T C -0.1099 Soluble
115 P C -0.5060 Soluble
116 P C 0.0000 Buried
117 T C -0.2969 Soluble
118 F C -0.4920 Soluble
119 G C 0.0000 Buried
120 Q C -1.9262 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -1.8601 Soluble
124 V C 0.0000 Buried
125 E C -0.9175 Soluble
126 I C 0.0000 Buried
127 K C -0.9210 Soluble
128 R C -0.7270 Soluble
129 T C -0.0596 Soluble
130 V C 0.4386 Aggregation-prone
131 A C 0.0370 Aggregation-prone
132 A C -0.1127 Soluble
133 P C 0.0000 Buried
134 S C -0.1566 Soluble
135 V C 0.0000 Buried
136 F C 0.1469 Aggregation-prone
137 I C 0.1683 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.4999 Soluble
140 P C 0.0000 Buried
141 S C -1.6622 Soluble
142 D C -2.9983 Soluble
143 E C -2.9381 Soluble
144 Q C 0.0000 Buried
145 L C -2.3023 Soluble
146 K C -2.9240 Soluble
147 S C -1.8849 Soluble
148 G C -1.5420 Soluble
149 T C -1.1228 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 K C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C -0.3447 Soluble
158 N C -0.7735 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.2918 Soluble
162 R C -2.1024 Soluble
163 E C -2.7147 Soluble
164 A C -1.8854 Soluble
165 K C -1.8778 Soluble
166 V C -0.8534 Soluble
167 Q C -0.5598 Soluble
168 W C 0.0000 Buried
169 K C -0.6862 Soluble
170 V C 0.0000 Buried
171 D C -2.0950 Soluble
172 N C -1.6427 Soluble
173 A C -0.3453 Soluble
174 L C 0.6838 Aggregation-prone
175 Q C -0.3981 Soluble
176 S C -0.7118 Soluble
177 G C -1.3787 Soluble
178 N C -1.8097 Soluble
179 S C -1.5770 Soluble
180 Q C -1.5419 Soluble
181 E C -1.6315 Soluble
182 S C -0.7906 Soluble
183 V C -0.4174 Soluble
184 T C -0.9579 Soluble
185 E C -1.9474 Soluble
186 Q C 0.0000 Buried
187 D C -1.9290 Soluble
188 S C -1.9342 Soluble
189 K C -2.3650 Soluble
190 D C -1.9711 Soluble
191 S C -1.6950 Soluble
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.7556 Soluble
199 L C 0.0000 Buried
200 T C -0.8208 Soluble
201 L C -0.8828 Soluble
202 S C -1.3221 Soluble
203 K C -2.0232 Soluble
204 A C -1.8953 Soluble
205 D C -2.6136 Soluble
206 Y C 0.0000 Buried
207 E C -3.3761 Soluble
208 K C -3.6251 Soluble
209 H C -3.0170 Soluble
210 K C -3.3215 Soluble
211 V C -1.6048 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.8873 Soluble
216 V C 0.0000 Buried
217 T C -1.0399 Soluble
218 H C 0.0000 Buried
219 Q C -1.5801 Soluble
220 G C -0.4090 Soluble
221 L C -0.2392 Soluble
222 S C -0.4438 Soluble
223 S C -0.4285 Soluble
224 P C -0.5115 Soluble
225 V C -0.0920 Soluble
226 T C -0.4721 Soluble
227 K C -0.6621 Soluble
228 S C -0.5215 Soluble
229 F C -0.8354 Soluble
230 N C -1.8654 Soluble
231 R C -2.4468 Soluble
232 G C -1.9605 Soluble
233 E C -2.1627 Soluble
234 C C -0.3907 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6711 3.1007 CSV PDB
4.5 -0.716 3.0106 CSV PDB
5.0 -0.7684 2.8968 CSV PDB
5.5 -0.8187 2.7731 CSV PDB
6.0 -0.8556 2.6464 CSV PDB
6.5 -0.87 2.5179 CSV PDB
7.0 -0.8615 2.4136 CSV PDB
7.5 -0.8373 2.4093 CSV PDB
8.0 -0.804 2.4077 CSV PDB
8.5 -0.7637 2.407 CSV PDB
9.0 -0.7163 2.4066 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH