Sudubrilimab / 5x8l

A4DAB248_5x8l_G_L | Source PDB: 5x8l | Chains: G, L | Done
97.33
Yes
10 Å
Yes
Avg A4D Score
-0.669
Max Score
2.1263
Min Score
-4.1541
Total Score
-276.32
Score CDR
-0.2149
Score non-CDR
-0.7246

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSDSWIHWVRQAPGKGLEWVAWISPYGGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARRHWPGGFDYWGQGTLVTVSSASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYLYHPATFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) G (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.1541
Maximum Score 2.1263
Average Score -0.669
Total Score -276.316

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E G -2.0473 Soluble
2 V G -1.1327 Soluble
3 Q G -0.9903 Soluble
4 L G 0.0000 Buried
5 V G 1.0269 Aggregation-prone
6 E G 0.0000 Buried
7 S G -0.3846 Soluble
8 G G -1.0298 Soluble
9 G G -0.4365 Soluble
11 G G -0.1405 Soluble
12 L G 0.1621 Aggregation-prone
13 V G -0.4328 Soluble
14 Q G -1.4072 Soluble
15 P G -1.5268 Soluble
16 G G -1.4044 Soluble
17 G G -1.1116 Soluble
18 S G -1.4087 Soluble
19 L G -1.4013 Soluble
20 R G -2.4814 Soluble
21 L G 0.0000 Buried
22 S G -0.5219 Soluble
23 C G 0.0000 Buried
24 A G -0.0508 Soluble
25 A G 0.0000 Buried
26 S G -0.7736 Soluble
27 G G -1.1283 Soluble
28 F G -0.7158 Soluble
29 T G -0.6578 Soluble
30 F G 0.0000 Buried
35 S G -0.6624 Soluble
36 D G -1.4927 Soluble
37 S G 0.0000 Buried
38 W G 0.0001 Aggregation-prone
39 I G 0.0000 Buried
40 H G 0.0000 Buried
41 W G 0.0000 Buried
42 V G 0.0000 Buried
43 R G 0.0000 Buried
44 Q G -0.8190 Soluble
45 A G -1.1680 Soluble
46 P G -1.1217 Soluble
47 G G -1.9449 Soluble
48 K G -2.5323 Soluble
49 G G -1.6964 Soluble
50 L G 0.0000 Buried
51 E G -1.3956 Soluble
52 W G 0.0000 Buried
53 V G 0.0000 Buried
54 A G 0.0000 Buried
55 W G 0.5258 Aggregation-prone
56 I G 0.0000 Buried
57 S G -0.0123 Soluble
58 P G 0.0000 Buried
59 Y G 0.4256 Aggregation-prone
62 G G -0.2350 Soluble
63 G G -0.3472 Soluble
64 S G 0.0092 Aggregation-prone
65 T G 0.4299 Aggregation-prone
66 Y G 0.9443 Aggregation-prone
67 Y G -0.2769 Soluble
68 A G -1.3459 Soluble
69 D G -2.3582 Soluble
70 S G -1.7793 Soluble
71 V G 0.0000 Buried
72 K G -2.4404 Soluble
74 G G -1.8473 Soluble
75 R G -1.9763 Soluble
76 F G 0.0000 Buried
77 T G -1.0554 Soluble
78 I G 0.0000 Buried
79 S G -0.5624 Soluble
80 A G -0.8066 Soluble
81 D G -1.4762 Soluble
82 T G -1.0997 Soluble
83 S G -1.4748 Soluble
84 K G -2.3171 Soluble
85 N G -1.6529 Soluble
86 T G 0.0000 Buried
87 A G 0.0000 Buried
88 Y G 0.0000 Buried
89 L G 0.0000 Buried
90 Q G -2.0776 Soluble
91 M G 0.0000 Buried
92 N G -2.0173 Soluble
93 S G -1.3892 Soluble
94 L G 0.0000 Buried
95 R G -2.0969 Soluble
96 A G -1.6233 Soluble
97 E G -2.2039 Soluble
98 D G 0.0000 Buried
99 T G -0.5842 Soluble
100 A G 0.0000 Buried
101 V G 0.4294 Aggregation-prone
102 Y G 0.0000 Buried
103 Y G 0.0000 Buried
104 C G 0.0000 Buried
105 A G 0.0000 Buried
106 R G -0.0515 Soluble
107 R G 0.0987 Aggregation-prone
108 H G 0.3831 Aggregation-prone
109 W G 0.9624 Aggregation-prone
110 P G 0.5011 Aggregation-prone
113 G G 0.0000 Buried
114 G G 0.0000 Buried
115 F G 0.0000 Buried
116 D G 0.0000 Buried
117 Y G -0.1692 Soluble
118 W G 0.0000 Buried
119 G G 0.0000 Buried
120 Q G -1.1061 Soluble
121 G G -0.4728 Soluble
122 T G 0.0000 Buried
123 L G 0.5792 Aggregation-prone
124 V G 0.0000 Buried
125 T G 0.0000 Buried
126 V G 0.0000 Buried
127 S G -0.8546 Soluble
128 S G -0.6561 Soluble
129 A G -0.4297 Soluble
130 S G -0.5198 Soluble
131 T G -0.6780 Soluble
132 K G -1.0039 Soluble
133 G G -1.2823 Soluble
134 P G 0.0000 Buried
135 S G -0.3804 Soluble
136 V G 0.0000 Buried
137 F G 0.0000 Buried
138 P G -1.2352 Soluble
139 L G 0.0000 Buried
140 A G -0.5570 Soluble
141 P G -0.4614 Soluble
142 G G -0.6079 Soluble
143 T G -0.4518 Soluble
144 A G -0.1980 Soluble
145 A G 0.0000 Buried
146 L G 0.0000 Buried
147 G G 0.0000 Buried
148 C G 0.0000 Buried
149 L G 0.0000 Buried
150 V G 0.0000 Buried
151 K G 0.0000 Buried
152 D G -0.2763 Soluble
153 Y G 0.0000 Buried
154 F G 0.0000 Buried
155 P G 0.0000 Buried
156 E G -0.7187 Soluble
157 P G -0.8457 Soluble
158 V G -0.7275 Soluble
159 T G -0.5356 Soluble
160 V G -0.1676 Soluble
161 S G -0.3642 Soluble
162 W G 0.0000 Buried
163 N G -0.7408 Soluble
164 S G -0.6490 Soluble
165 G G -0.5053 Soluble
166 A G -0.2166 Soluble
167 L G 0.0688 Aggregation-prone
168 T G -0.0943 Soluble
169 S G -0.2994 Soluble
170 G G -0.2922 Soluble
171 V G 0.2912 Aggregation-prone
172 H G 0.0000 Buried
173 T G 0.0724 Aggregation-prone
174 F G 0.0000 Buried
175 P G -0.2301 Soluble
176 A G 0.2564 Aggregation-prone
177 V G 0.6336 Aggregation-prone
178 L G 1.0098 Aggregation-prone
179 Q G 0.2067 Aggregation-prone
180 S G -0.2919 Soluble
181 S G -0.2355 Soluble
182 G G -0.0397 Soluble
183 L G 0.1025 Aggregation-prone
184 Y G 0.3963 Aggregation-prone
185 S G 0.0000 Buried
186 L G 0.0000 Buried
187 S G 0.0000 Buried
188 S G 0.0000 Buried
189 V G 0.0000 Buried
190 V G 0.0000 Buried
191 T G -0.0551 Soluble
192 V G 0.0000 Buried
193 P G -0.4420 Soluble
194 S G -0.4729 Soluble
195 S G -0.5772 Soluble
196 S G -0.6496 Soluble
197 L G -0.8241 Soluble
198 G G -1.2243 Soluble
199 T G -1.0531 Soluble
200 Q G -1.5210 Soluble
201 T G -1.0638 Soluble
202 Y G 0.0000 Buried
203 I G -1.4411 Soluble
204 C G 0.0000 Buried
205 N G -1.4565 Soluble
206 V G 0.0000 Buried
207 N G -1.8733 Soluble
208 H G 0.0000 Buried
209 K G -2.7675 Soluble
210 P G -1.6239 Soluble
211 S G -1.8142 Soluble
212 N G -2.5934 Soluble
213 T G -2.0703 Soluble
214 K G -2.6834 Soluble
215 V G -1.6647 Soluble
216 D G -2.5662 Soluble
217 K G -2.0968 Soluble
218 K G -2.5704 Soluble
219 V G 0.0000 Buried
220 E G -2.5054 Soluble
221 P G -1.2014 Soluble
1 D L -2.1967 Soluble
2 I L -1.7002 Soluble
3 Q L -2.2208 Soluble
4 M L 0.0000 Buried
5 T L -1.4563 Soluble
6 Q L 0.0000 Buried
7 S L -0.7806 Soluble
8 P L -0.6418 Soluble
9 S L -0.8911 Soluble
10 S L -0.7966 Soluble
11 L L -0.5877 Soluble
12 S L -0.8737 Soluble
13 A L -0.9079 Soluble
14 S L -0.8296 Soluble
15 V L -0.0761 Soluble
16 G L -0.8854 Soluble
17 D L -1.7633 Soluble
18 R L -2.3466 Soluble
19 V L 0.0000 Buried
20 T L -0.6275 Soluble
21 I L 0.0000 Buried
22 T L -0.7932 Soluble
23 C L 0.0000 Buried
24 R L -2.8472 Soluble
25 A L 0.0000 Buried
26 S L -2.2030 Soluble
27 Q L -2.8745 Soluble
28 D L -2.6761 Soluble
29 V L 0.0000 Buried
36 S L -1.0180 Soluble
37 T L -0.5210 Soluble
38 A L 0.0000 Buried
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.7820 Soluble
46 P L -1.6239 Soluble
47 G L -1.7017 Soluble
48 K L -2.6515 Soluble
49 A L -1.6318 Soluble
50 P L 0.0000 Buried
51 K L -1.5539 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.0526 Aggregation-prone
56 S L 0.5369 Aggregation-prone
57 A L 0.0000 Buried
65 S L 0.7651 Aggregation-prone
66 F L 2.1263 Aggregation-prone
67 L L 1.3668 Aggregation-prone
68 Y L 0.5653 Aggregation-prone
69 S L 0.0118 Aggregation-prone
70 G L -0.4920 Soluble
71 V L 0.0000 Buried
72 P L -0.3987 Soluble
74 S L -0.4599 Soluble
75 R L -0.8049 Soluble
76 F L 0.0000 Buried
77 S L 0.0532 Aggregation-prone
78 G L 0.0000 Buried
79 S L -0.2708 Soluble
80 G L -0.9377 Soluble
83 S L -1.0299 Soluble
84 G L -1.7521 Soluble
85 T L -2.2181 Soluble
86 D L -2.1864 Soluble
87 F L 0.0000 Buried
88 T L -0.6876 Soluble
89 L L 0.0000 Buried
90 T L -0.5944 Soluble
91 I L 0.0000 Buried
92 S L -1.3551 Soluble
93 S L -1.2643 Soluble
94 L L 0.0000 Buried
95 Q L -1.0756 Soluble
96 P L -1.0323 Soluble
97 E L -1.8743 Soluble
98 D L 0.0000 Buried
99 F L -0.6024 Soluble
100 A L 0.0000 Buried
101 T L -0.9261 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.6194 Aggregation-prone
108 L L 0.8257 Aggregation-prone
109 Y L 0.3361 Aggregation-prone
114 H L -0.7116 Soluble
115 P L -0.9851 Soluble
116 A L -0.4257 Soluble
117 T L -0.8788 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.8767 Soluble
121 G L -1.2362 Soluble
122 T L 0.0000 Buried
123 K L -1.5124 Soluble
124 V L 0.0000 Buried
125 E L -1.1733 Soluble
126 I L -0.6867 Soluble
127 K L -1.3702 Soluble
128 R L -0.8699 Soluble
129 T L 0.0310 Aggregation-prone
130 V L 0.7448 Aggregation-prone
131 A L 0.2160 Aggregation-prone
132 A L 0.0187 Aggregation-prone
133 P L -0.1065 Soluble
134 S L -0.0731 Soluble
135 V L -0.0137 Soluble
136 F L 0.0125 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.4473 Soluble
140 P L -0.9045 Soluble
141 S L -1.5984 Soluble
142 D L -3.0601 Soluble
143 E L -2.8127 Soluble
144 Q L 0.0000 Buried
145 L L -2.2762 Soluble
146 K L -2.9053 Soluble
147 S L -1.7086 Soluble
148 G L -1.5653 Soluble
149 T L -0.8827 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7691 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6476 Soluble
162 R L -2.9639 Soluble
163 E L -3.1173 Soluble
164 A L -2.3171 Soluble
165 K L -2.7536 Soluble
166 V L 0.0000 Buried
167 Q L -1.6887 Soluble
168 W L -0.3393 Soluble
169 N L -1.2345 Soluble
170 S L -0.9725 Soluble
171 Q L -0.9720 Soluble
172 E L -0.9199 Soluble
173 S L -0.3073 Soluble
174 V L -0.5313 Soluble
175 T L -1.1476 Soluble
176 E L -2.2744 Soluble
177 Q L -2.1275 Soluble
178 D L -1.9958 Soluble
179 S L -1.7910 Soluble
180 K L -2.4589 Soluble
181 D L -1.9985 Soluble
182 S L -1.8770 Soluble
183 T L 0.0000 Buried
184 Y L 0.0000 Buried
185 S L 0.0000 Buried
186 L L 0.0000 Buried
187 S L 0.0000 Buried
188 S L 0.0000 Buried
189 T L -0.3422 Soluble
190 L L 0.0123 Aggregation-prone
191 T L -0.0876 Soluble
192 L L 0.1422 Aggregation-prone
193 S L -1.1984 Soluble
194 K L -2.7993 Soluble
195 A L -2.3976 Soluble
196 D L -3.3370 Soluble
197 Y L -3.0621 Soluble
198 E L -4.1541 Soluble
199 K L -4.0227 Soluble
200 H L -3.4240 Soluble
201 K L -2.7082 Soluble
202 V L -0.7312 Soluble
203 Y L -0.1472 Soluble
204 A L -0.3528 Soluble
205 C L 0.0000 Buried
206 E L -1.6135 Soluble
207 V L 0.0000 Buried
208 T L -1.2770 Soluble
209 H L 0.0000 Buried
210 Q L -1.4959 Soluble
211 G L -0.4382 Soluble
212 L L -0.3130 Soluble
213 S L -0.3291 Soluble
214 S L -0.2365 Soluble
215 P L -0.2242 Soluble
216 V L 0.3406 Aggregation-prone
217 T L -0.5662 Soluble
218 K L -0.7697 Soluble
219 S L -0.1130 Soluble
220 F L 0.3827 Aggregation-prone
221 N L -1.2918 Soluble
222 R L -2.6198 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6337 4.0791 CSV PDB
4.5 -0.6784 4.0924 CSV PDB
5.0 -0.7306 4.1065 CSV PDB
5.5 -0.7789 4.1161 CSV PDB
6.0 -0.8116 4.1205 CSV PDB
6.5 -0.8209 4.1221 CSV PDB
7.0 -0.8086 4.1227 CSV PDB
7.5 -0.7829 4.1229 CSV PDB
8.0 -0.7503 4.1229 CSV PDB
8.5 -0.7123 4.1229 CSV PDB
9.0 -0.6676 4.1228 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH