Lotivibart / 5te4

A4DAB160_5te4_H_L | Source PDB: 5te4 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7034
Max Score
1.0947
Min Score
-3.7165
Total Score
-305.3
Score CDR
-0.3929
Score non-CDR
-0.7394

Calculation Parameters

Chain Sequence(s) RAHLVQSGTAMKKPGASVRVSCQTSGYTFTAHILFWFRQAPGRGLEWVGWIKPQYGAVNFGGGFRDRVTLTRDVYREIAYMDIRGLKPDDTAVYYCARDRSYGDSSWALDAWGQGTTVVVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSYIHVTQSPSSLSVSIGDRVTINCQTSQGVGSDLHWYQHKPGRAPKLLIHHTSSVEDGVPSRFSGSGFHTSFNLTISDLQADDIATYYCQVLQFFGRGSRLHIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7165
Maximum Score 1.0947
Average Score -0.7034
Total Score -305.2952

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 R H -2.1855 Soluble
2 A H -1.6184 Soluble
3 H H -1.5782 Soluble
4 L H 0.0000 Buried
5 V H 0.0223 Aggregation-prone
6 Q H -0.2175 Soluble
7 S H -0.6322 Soluble
8 G H -0.5741 Soluble
9 T H -0.2083 Soluble
11 A H -0.2436 Soluble
12 M H 0.0394 Aggregation-prone
13 K H -0.9431 Soluble
14 K H -1.5891 Soluble
15 P H -1.8596 Soluble
16 G H -1.6524 Soluble
17 A H -1.2695 Soluble
18 S H -1.6621 Soluble
19 V H 0.0000 Buried
20 R H -2.0647 Soluble
21 V H 0.0000 Buried
22 S H -0.4979 Soluble
23 C H 0.0000 Buried
24 Q H -0.7583 Soluble
25 T H 0.0000 Buried
26 S H -1.1824 Soluble
27 G H -1.1391 Soluble
28 Y H -0.6565 Soluble
29 T H 0.0299 Aggregation-prone
30 F H 0.0000 Buried
35 T H -0.2067 Soluble
36 A H -0.4657 Soluble
37 H H 0.0000 Buried
38 I H 0.0000 Buried
39 L H 0.0000 Buried
40 F H 0.0000 Buried
41 W H 0.0000 Buried
42 F H 0.0000 Buried
43 R H -0.6537 Soluble
44 Q H -0.9830 Soluble
45 A H -1.3036 Soluble
46 P H -1.2635 Soluble
47 G H -1.7412 Soluble
48 R H -2.7467 Soluble
49 G H -2.0210 Soluble
50 L H 0.0000 Buried
51 E H -1.2563 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 W H -0.0236 Soluble
56 I H 0.0000 Buried
57 K H -0.8573 Soluble
58 P H 0.0000 Buried
59 Q H -0.4863 Soluble
62 Y H 0.7917 Aggregation-prone
63 G H 0.0463 Aggregation-prone
64 A H 0.1867 Aggregation-prone
65 V H 0.2789 Aggregation-prone
66 N H -0.5518 Soluble
67 F H -0.8329 Soluble
68 G H 0.0000 Buried
69 G H -1.2907 Soluble
70 G H -1.6698 Soluble
71 F H -1.7403 Soluble
72 R H -2.9253 Soluble
74 D H -3.1127 Soluble
75 R H -2.2372 Soluble
76 V H 0.0000 Buried
77 T H -0.9850 Soluble
78 L H 0.0000 Buried
79 T H -0.1401 Soluble
80 R H -0.8780 Soluble
81 D H -1.1313 Soluble
82 V H -0.0113 Soluble
83 Y H 0.2354 Aggregation-prone
84 R H -1.6646 Soluble
85 E H -1.2657 Soluble
86 I H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5111 Soluble
89 M H 0.0000 Buried
90 D H -1.2501 Soluble
91 I H 0.0000 Buried
92 R H -2.2501 Soluble
93 G H -1.8413 Soluble
94 L H 0.0000 Buried
95 K H -2.7172 Soluble
96 P H -2.0732 Soluble
97 D H -2.4140 Soluble
98 D H 0.0000 Buried
99 T H -0.9042 Soluble
100 A H 0.0000 Buried
101 V H 0.0488 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.5347 Soluble
107 D H 0.0000 Buried
108 R H -0.7845 Soluble
109 S H -0.4638 Soluble
110 Y H 0.3434 Aggregation-prone
111 G H -0.9079 Soluble
111A D H -1.7558 Soluble
112A S H -0.9724 Soluble
112 S H -0.7585 Soluble
113 W H -0.4262 Soluble
114 A H 0.0000 Buried
115 L H 0.0000 Buried
116 D H -1.0428 Soluble
117 A H 0.0000 Buried
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.3874 Soluble
121 G H -0.7611 Soluble
122 T H 0.0000 Buried
123 T H -0.0982 Soluble
124 V H 0.0000 Buried
125 V H -0.4454 Soluble
126 V H 0.0000 Buried
127 S H -0.9258 Soluble
128 A H -0.6902 Soluble
129 A H -0.4880 Soluble
130 S H -0.5957 Soluble
131 T H -0.6837 Soluble
132 K H -1.2476 Soluble
133 G H -1.4003 Soluble
134 P H -0.5969 Soluble
135 S H -0.4178 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2132 Soluble
139 L H 0.0000 Buried
140 A H -1.3036 Soluble
141 P H 0.0000 Buried
142 S H -1.0033 Soluble
143 S H -0.7537 Soluble
144 K H -0.8294 Soluble
145 S H 0.0000 Buried
146 T H -0.7279 Soluble
147 S H -0.7856 Soluble
148 G H -0.9256 Soluble
149 G H -0.8337 Soluble
150 T H -0.5615 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.3930 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H -0.7327 Soluble
163 E H -1.0403 Soluble
164 P H -1.1272 Soluble
165 V H 0.0000 Buried
166 T H -0.7021 Soluble
167 V H -0.3655 Soluble
168 S H -0.3520 Soluble
169 W H 0.0000 Buried
170 N H -0.7595 Soluble
171 S H -0.6664 Soluble
172 G H -0.5079 Soluble
173 A H -0.2680 Soluble
174 L H -0.0313 Soluble
175 T H -0.1876 Soluble
176 S H -0.1899 Soluble
177 G H -0.2117 Soluble
178 V H 0.1354 Aggregation-prone
179 H H -0.3045 Soluble
180 T H -0.1710 Soluble
181 F H 0.0000 Buried
182 P H -0.4250 Soluble
183 A H 0.0409 Aggregation-prone
184 V H 0.3791 Aggregation-prone
185 L H 1.0947 Aggregation-prone
186 Q H 0.2837 Aggregation-prone
187 S H -0.0844 Soluble
188 S H -0.1814 Soluble
189 G H 0.0844 Aggregation-prone
190 L H 0.0484 Aggregation-prone
191 Y H 0.2918 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1243 Soluble
199 V H 0.0000 Buried
200 P H -0.4775 Soluble
201 S H -0.5807 Soluble
202 S H -0.5873 Soluble
203 S H -0.6341 Soluble
204 L H -0.8276 Soluble
205 G H -1.0171 Soluble
206 T H -0.8291 Soluble
207 Q H -1.4123 Soluble
208 T H -1.2037 Soluble
209 Y H 0.0000 Buried
210 I H -1.0970 Soluble
211 C H 0.0000 Buried
212 N H -1.2643 Soluble
213 V H 0.0000 Buried
214 N H -1.7784 Soluble
215 H H 0.0000 Buried
216 K H -2.7968 Soluble
217 P H -1.7475 Soluble
218 S H -1.8496 Soluble
219 N H -2.5589 Soluble
220 T H -2.0336 Soluble
221 K H -2.5450 Soluble
222 V H -1.3155 Soluble
223 D H -2.0775 Soluble
224 K H -1.7393 Soluble
225 K H -2.0842 Soluble
226 V H 0.0000 Buried
227 E H -2.8356 Soluble
228 P H -2.0053 Soluble
229 K H -2.5347 Soluble
230 S H -1.3325 Soluble
1 Y L 0.9558 Aggregation-prone
2 I L 0.0000 Buried
3 H L -0.8892 Soluble
4 V L -0.8098 Soluble
5 T L -1.0048 Soluble
6 Q L 0.0000 Buried
7 S L -0.9260 Soluble
8 P L -0.5980 Soluble
9 S L -1.0853 Soluble
10 S L -0.9357 Soluble
11 L L -0.5400 Soluble
12 S L -0.8587 Soluble
13 V L 0.0000 Buried
14 S L -0.8008 Soluble
15 I L 0.3444 Aggregation-prone
16 G L -1.4693 Soluble
17 D L -2.5554 Soluble
18 R L -2.9133 Soluble
19 V L 0.0000 Buried
20 T L -0.8137 Soluble
21 I L 0.0000 Buried
22 N L -0.7834 Soluble
23 C L 0.0000 Buried
24 Q L -1.2900 Soluble
25 T L 0.0000 Buried
26 S L -1.1892 Soluble
27 Q L -1.5850 Soluble
28 G L -1.0621 Soluble
29 V L -0.6919 Soluble
36 G L -0.5939 Soluble
37 S L -0.9904 Soluble
38 D L -1.7128 Soluble
39 L L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 H L -0.8383 Soluble
45 K L -0.9985 Soluble
46 P L -0.8306 Soluble
47 G L -1.1299 Soluble
48 R L -1.3944 Soluble
49 A L -1.0453 Soluble
50 P L 0.0000 Buried
51 K L -0.9781 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 H L -0.6721 Soluble
56 H L -1.1877 Soluble
57 T L -0.5847 Soluble
65 S L -0.5534 Soluble
66 S L -0.2333 Soluble
67 V L 0.0820 Aggregation-prone
68 E L -1.0381 Soluble
69 D L -1.9390 Soluble
70 G L -1.2210 Soluble
71 V L -0.6697 Soluble
72 P L -0.4747 Soluble
74 S L -0.6956 Soluble
75 R L -1.2844 Soluble
76 F L 0.0000 Buried
77 S L -0.2051 Soluble
78 G L 0.0000 Buried
79 S L -0.2827 Soluble
80 G L 0.0578 Aggregation-prone
83 F L 0.9227 Aggregation-prone
84 H L -0.6159 Soluble
85 T L -0.6587 Soluble
86 S L -0.5032 Soluble
87 F L 0.0000 Buried
88 N L -0.6379 Soluble
89 L L 0.0000 Buried
90 T L -0.9027 Soluble
91 I L 0.0000 Buried
92 S L -2.2294 Soluble
93 D L -2.8890 Soluble
94 L L 0.0000 Buried
95 Q L -1.7543 Soluble
96 A L -0.8754 Soluble
97 D L -2.0471 Soluble
98 D L 0.0000 Buried
99 I L -0.4458 Soluble
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 V L 0.0000 Buried
107 L L 0.5605 Aggregation-prone
116 Q L 0.3077 Aggregation-prone
117 F L 0.1962 Aggregation-prone
118 F L -0.3151 Soluble
119 G L 0.0000 Buried
120 R L -2.5115 Soluble
121 G L 0.0000 Buried
122 S L 0.0000 Buried
123 R L -2.0263 Soluble
124 L L 0.0000 Buried
125 H L -1.2241 Soluble
126 I L -0.4296 Soluble
127 K L -1.5003 Soluble
128 R L -1.2008 Soluble
129 T L -0.1650 Soluble
130 V L 0.3513 Aggregation-prone
131 A L 0.1884 Aggregation-prone
132 A L -0.0830 Soluble
133 P L 0.0000 Buried
134 S L -0.1947 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.4558 Soluble
140 P L 0.0000 Buried
141 S L -1.8424 Soluble
142 D L -3.1899 Soluble
143 E L -3.0079 Soluble
144 Q L 0.0000 Buried
145 L L -2.4232 Soluble
146 K L -2.9542 Soluble
147 S L -1.8771 Soluble
148 G L -1.5001 Soluble
149 T L -1.0661 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8468 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.8725 Soluble
162 R L -3.1726 Soluble
163 E L -3.2683 Soluble
164 A L -2.3792 Soluble
165 K L -2.4925 Soluble
166 V L -1.2529 Soluble
167 Q L -0.8214 Soluble
168 W L 0.0000 Buried
169 K L -0.5639 Soluble
170 V L 0.0000 Buried
171 D L -1.6295 Soluble
172 N L -1.3598 Soluble
173 A L -0.2631 Soluble
174 L L 0.7329 Aggregation-prone
175 Q L -0.1113 Soluble
176 S L -0.4721 Soluble
177 G L -0.9126 Soluble
178 N L -0.8688 Soluble
179 S L -1.1667 Soluble
180 Q L -1.4306 Soluble
181 E L -1.9562 Soluble
182 S L -1.0345 Soluble
183 V L -0.8796 Soluble
184 T L -1.1342 Soluble
185 E L -2.2589 Soluble
186 Q L -1.8795 Soluble
187 D L -1.8082 Soluble
188 S L -1.6031 Soluble
189 K L -2.1361 Soluble
190 D L -1.5067 Soluble
191 S L -1.6708 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6875 Soluble
199 L L 0.0000 Buried
200 T L -0.3093 Soluble
201 L L -0.6880 Soluble
202 S L -1.1593 Soluble
203 K L -2.2548 Soluble
204 A L -2.0648 Soluble
205 D L -2.7144 Soluble
206 Y L 0.0000 Buried
207 E L -3.7165 Soluble
208 K L -3.6164 Soluble
209 H L -2.8537 Soluble
210 K L -2.6982 Soluble
211 V L -0.9901 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7933 Soluble
216 V L 0.0000 Buried
217 T L -1.1935 Soluble
218 H L 0.0000 Buried
219 Q L -1.7625 Soluble
220 G L -0.4083 Soluble
221 L L -0.2213 Soluble
222 S L -0.4442 Soluble
223 S L -0.3710 Soluble
224 P L -0.5037 Soluble
225 V L 0.1993 Aggregation-prone
226 T L -0.2287 Soluble
227 K L -0.3868 Soluble
228 S L -0.2552 Soluble
229 F L 0.0000 Buried
230 N L -1.4218 Soluble
231 R L -2.1781 Soluble
232 G L -1.9008 Soluble
233 E L -2.0887 Soluble
234 C L -0.7783 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.721 2.4675 CSV PDB
4.5 -0.7639 2.4525 CSV PDB
5.0 -0.8145 2.4579 CSV PDB
5.5 -0.8624 2.4997 CSV PDB
6.0 -0.8976 2.5599 CSV PDB
6.5 -0.9146 2.5968 CSV PDB
7.0 -0.9141 2.5944 CSV PDB
7.5 -0.9022 2.5671 CSV PDB
8.0 -0.8834 2.5304 CSV PDB
8.5 -0.8586 2.4933 CSV PDB
9.0 -0.8267 2.4629 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH