Anbenitamab / 7t98

A4DAB016_7t98_H_L | Source PDB: 7t98 | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6558
Max Score
2.1814
Min Score
-3.5852
Total Score
-283.33
Score CDR
-0.4857
Score non-CDR
-0.6766

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVCDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKCSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5852
Maximum Score 2.1814
Average Score -0.6558
Total Score -283.3268

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1441 Soluble
2 V H -1.3633 Soluble
3 Q H -1.4916 Soluble
4 L H 0.0000 Buried
5 V H 0.0538 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5101 Soluble
8 G H -0.7272 Soluble
9 G H -0.3790 Soluble
11 G H -0.4310 Soluble
12 L H -0.1174 Soluble
13 V H -0.6282 Soluble
14 Q H -1.5963 Soluble
15 P H -1.7513 Soluble
16 G H -1.5487 Soluble
17 G H -1.2908 Soluble
18 S H -1.2791 Soluble
19 L H -0.9337 Soluble
20 R H -1.6654 Soluble
21 L H 0.0000 Buried
22 S H -0.3739 Soluble
23 C H 0.0000 Buried
24 A H -0.3338 Soluble
25 A H -0.7077 Soluble
26 S H -1.1892 Soluble
27 G H -1.3777 Soluble
28 F H -1.2404 Soluble
29 N H -1.8368 Soluble
30 I H 0.0000 Buried
35 K H -2.1907 Soluble
36 D H -2.6782 Soluble
37 T H 0.0000 Buried
38 Y H -0.3219 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7622 Soluble
45 A H -1.1853 Soluble
46 P H -0.9552 Soluble
47 G H -1.4545 Soluble
48 K H -2.3928 Soluble
49 G H -1.6637 Soluble
50 L H 0.0000 Buried
51 E H -1.1589 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H -0.3504 Soluble
56 I H 0.0000 Buried
57 Y H -0.3690 Soluble
58 P H 0.0000 Buried
59 T H -1.2019 Soluble
62 N H -1.1642 Soluble
63 G H -0.5901 Soluble
64 Y H 0.5128 Aggregation-prone
65 T H -0.1930 Soluble
66 R H -1.3616 Soluble
67 Y H -1.5258 Soluble
68 A H 0.0000 Buried
69 D H -2.8064 Soluble
70 S H -1.7729 Soluble
71 V H 0.0000 Buried
72 K H -2.7687 Soluble
74 G H -1.7895 Soluble
75 R H -1.7881 Soluble
76 F H 0.0000 Buried
77 T H -1.1951 Soluble
78 I H 0.0000 Buried
79 S H -0.1935 Soluble
80 A H -0.6965 Soluble
81 D H -1.2749 Soluble
82 T H -1.3526 Soluble
83 S H -1.3631 Soluble
84 K H -2.1700 Soluble
85 N H -1.6287 Soluble
86 T H -0.8624 Soluble
87 A H 0.0000 Buried
88 Y H -0.4848 Soluble
89 L H 0.0000 Buried
90 Q H -1.5629 Soluble
91 M H 0.0000 Buried
92 N H -2.1376 Soluble
93 S H -1.5346 Soluble
94 L H 0.0000 Buried
95 R H -2.4803 Soluble
96 A H -1.7312 Soluble
97 E H -2.2989 Soluble
98 D H 0.0000 Buried
99 T H -0.7102 Soluble
100 A H 0.0000 Buried
101 V H 0.1540 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H -0.0204 Soluble
107 W H 0.0000 Buried
108 G H -0.4597 Soluble
109 G H -1.5556 Soluble
110 D H -2.0828 Soluble
111 G H -0.9306 Soluble
112 F H 0.0016 Aggregation-prone
113 Y H 0.5978 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.0064 Soluble
117 Y H 0.0196 Aggregation-prone
118 W H -0.5296 Soluble
119 G H 0.0000 Buried
120 Q H -1.6170 Soluble
121 G H -0.7574 Soluble
122 T H 0.0000 Buried
123 L H 0.0787 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4328 Soluble
126 V H 0.0000 Buried
127 S H -0.8196 Soluble
128 S H -0.7030 Soluble
129 A H -0.5284 Soluble
130 S H -0.7221 Soluble
131 T H -0.8738 Soluble
132 K H -1.5535 Soluble
133 G H -1.7138 Soluble
134 P H 0.0000 Buried
135 S H -0.5876 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0217 Soluble
139 L H 0.0000 Buried
140 A H -0.8716 Soluble
141 P H -0.6657 Soluble
142 S H -0.8724 Soluble
143 S H -0.8750 Soluble
144 K H -1.4656 Soluble
145 S H 0.0000 Buried
146 T H -0.8136 Soluble
147 S H -0.7950 Soluble
148 G H -0.7551 Soluble
149 G H -0.6244 Soluble
150 T H -0.4285 Soluble
151 A H -0.4725 Soluble
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.2376 Soluble
159 D H -0.5938 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.7796 Soluble
164 P H -0.8548 Soluble
165 V H -0.6876 Soluble
166 T H -0.5849 Soluble
167 V H -0.2803 Soluble
168 S H -0.3217 Soluble
169 W H 0.0000 Buried
170 N H -0.6370 Soluble
171 S H -0.6218 Soluble
172 G H -0.5458 Soluble
173 A H -0.2312 Soluble
174 L H -0.0119 Soluble
175 T H -0.1553 Soluble
176 S H -0.1524 Soluble
177 G H -0.0948 Soluble
178 V H 0.1930 Aggregation-prone
179 H H -0.3176 Soluble
180 T H -0.0499 Soluble
181 F H 0.0000 Buried
182 P H -0.2860 Soluble
183 A H 0.1123 Aggregation-prone
184 V H 0.3901 Aggregation-prone
185 L H 0.7735 Aggregation-prone
186 Q H 0.0717 Aggregation-prone
187 S H -0.2166 Soluble
188 S H -0.2347 Soluble
189 G H -0.0101 Soluble
190 L H -0.0071 Soluble
191 Y H 0.1966 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H 0.0128 Aggregation-prone
199 V H 0.1969 Aggregation-prone
200 P H -0.1755 Soluble
201 S H -0.1726 Soluble
202 S H -0.1455 Soluble
203 S H 0.0298 Aggregation-prone
204 L H 0.6811 Aggregation-prone
205 G H -0.2838 Soluble
206 T H -0.4480 Soluble
207 Q H -0.9447 Soluble
208 T H -0.7146 Soluble
209 Y H 0.0000 Buried
210 I H -0.7802 Soluble
211 C H 0.0000 Buried
212 N H -1.1746 Soluble
213 V H 0.0000 Buried
214 N H -1.6525 Soluble
215 H H 0.0000 Buried
216 K H -2.7890 Soluble
217 P H -1.7735 Soluble
218 S H -1.9113 Soluble
219 N H -2.5139 Soluble
220 T H -2.1361 Soluble
221 K H -2.4493 Soluble
222 V H -1.0625 Soluble
223 D H -1.7127 Soluble
224 K H -1.4677 Soluble
225 K H -1.2793 Soluble
226 V H 0.0000 Buried
227 E H -1.7475 Soluble
228 P H -1.2543 Soluble
229 K H -2.1892 Soluble
1 D L -2.4236 Soluble
2 I L 0.0000 Buried
3 Q L -2.2272 Soluble
4 M L 0.0000 Buried
5 T L -1.3176 Soluble
6 Q L 0.0000 Buried
7 S L -0.7574 Soluble
8 P L -0.6825 Soluble
9 S L -0.8972 Soluble
10 S L -0.8171 Soluble
11 L L -0.5917 Soluble
12 S L -0.7650 Soluble
13 A L -0.6904 Soluble
14 S L -0.5152 Soluble
15 V L 0.4964 Aggregation-prone
16 C L 0.3697 Aggregation-prone
17 D L -0.9016 Soluble
18 R L -1.9484 Soluble
19 V L 0.0000 Buried
20 T L -0.5885 Soluble
21 I L 0.0000 Buried
22 T L -0.7150 Soluble
23 C L 0.0000 Buried
24 R L -2.5183 Soluble
25 A L 0.0000 Buried
26 S L -1.9554 Soluble
27 Q L -2.2064 Soluble
28 D L -2.5069 Soluble
29 V L 0.0000 Buried
36 N L -1.4485 Soluble
37 T L -0.5666 Soluble
38 A L 0.0032 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.5976 Soluble
46 P L -1.3060 Soluble
47 G L -1.5177 Soluble
48 K L -2.5402 Soluble
49 A L -1.6079 Soluble
50 P L 0.0000 Buried
51 K L -1.7354 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.2624 Aggregation-prone
56 S L 0.6412 Aggregation-prone
57 A L 0.0000 Buried
65 S L 0.6578 Aggregation-prone
66 F L 2.1814 Aggregation-prone
67 L L 1.4908 Aggregation-prone
68 Y L 0.6127 Aggregation-prone
69 S L 0.0730 Aggregation-prone
70 G L -0.3816 Soluble
71 V L -0.2156 Soluble
72 P L -0.2641 Soluble
74 S L -0.3718 Soluble
75 R L -0.7459 Soluble
76 F L 0.0000 Buried
77 S L 0.1055 Aggregation-prone
78 G L 0.1348 Aggregation-prone
79 S L -0.6042 Soluble
80 R L -1.5911 Soluble
83 S L -1.2840 Soluble
84 G L -1.6959 Soluble
85 T L -2.0016 Soluble
86 D L -1.8185 Soluble
87 F L 0.0000 Buried
88 T L -0.7054 Soluble
89 L L 0.0000 Buried
90 T L -0.6427 Soluble
91 I L 0.0000 Buried
92 S L -1.0559 Soluble
93 S L -0.4936 Soluble
94 L L 0.0000 Buried
95 Q L -0.5527 Soluble
96 P L -1.1223 Soluble
97 E L -1.5610 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.7977 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.5450 Aggregation-prone
108 Y L 0.8200 Aggregation-prone
109 T L 0.2972 Aggregation-prone
114 T L -0.3172 Soluble
115 P L -0.9155 Soluble
116 P L 0.0000 Buried
117 T L -0.7416 Soluble
118 F L -0.5526 Soluble
119 G L 0.0000 Buried
120 Q L -1.9085 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.4261 Soluble
124 V L 0.0000 Buried
125 E L -1.1118 Soluble
126 I L 0.0000 Buried
127 K L -1.1931 Soluble
128 R L -0.6862 Soluble
129 T L 0.0592 Aggregation-prone
130 V L 0.8875 Aggregation-prone
131 A L 0.3114 Aggregation-prone
132 A L -0.0222 Soluble
133 P L 0.0000 Buried
134 S L -0.2479 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6296 Soluble
140 P L 0.0000 Buried
141 S L -1.9410 Soluble
142 D L -3.1394 Soluble
143 E L -3.2655 Soluble
144 Q L 0.0000 Buried
145 L L 0.0000 Buried
146 K L -3.1209 Soluble
147 S L -1.9649 Soluble
148 G L -1.7090 Soluble
149 T L -1.4110 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.5846 Soluble
159 F L 0.0000 Buried
160 Y L -0.6106 Soluble
161 P L -1.2231 Soluble
162 R L -2.3177 Soluble
163 E L -2.8379 Soluble
164 A L -2.2214 Soluble
165 K L -2.4866 Soluble
166 V L -1.4507 Soluble
167 Q L -1.4678 Soluble
168 W L 0.0000 Buried
169 K L -1.2387 Soluble
170 V L -0.9182 Soluble
171 D L -1.3061 Soluble
172 N L -1.3710 Soluble
173 A L -0.8614 Soluble
174 L L -0.4005 Soluble
175 Q L -1.1471 Soluble
177 G L -0.9368 Soluble
178 N L -0.9212 Soluble
179 S L -1.1779 Soluble
180 Q L -1.1488 Soluble
181 E L -1.3577 Soluble
182 S L 0.0000 Buried
183 V L -0.4608 Soluble
184 T L -1.1447 Soluble
185 E L -2.0895 Soluble
186 Q L 0.0000 Buried
187 D L -1.8119 Soluble
188 S L -1.8516 Soluble
189 K L -2.0750 Soluble
190 C L -1.0028 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.4773 Soluble
199 L L 0.0000 Buried
200 T L -0.3684 Soluble
201 L L -0.4865 Soluble
202 S L -1.0739 Soluble
203 K L -2.1877 Soluble
204 A L -1.8493 Soluble
205 D L -2.6409 Soluble
206 Y L 0.0000 Buried
207 E L -3.5852 Soluble
208 K L -3.5146 Soluble
209 H L -2.7091 Soluble
210 K L -2.5095 Soluble
211 V L -0.7861 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9160 Soluble
216 V L 0.0000 Buried
217 T L -1.0370 Soluble
218 H L 0.0000 Buried
219 Q L -1.4203 Soluble
220 G L -0.3051 Soluble
221 L L -0.0652 Soluble
222 S L -0.3677 Soluble
223 S L -0.2190 Soluble
224 P L -0.1273 Soluble
225 V L 0.4130 Aggregation-prone
226 T L -0.2542 Soluble
227 K L -0.6266 Soluble
228 S L -0.6711 Soluble
229 F L 0.0000 Buried
230 N L -1.9335 Soluble
231 R L -1.8719 Soluble
232 G L -1.4997 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6974 3.8056 CSV PDB
4.5 -0.7363 3.8056 CSV PDB
5.0 -0.7802 3.8056 CSV PDB
5.5 -0.8202 3.8056 CSV PDB
6.0 -0.846 3.8056 CSV PDB
6.5 -0.8505 3.8056 CSV PDB
7.0 -0.8345 3.8056 CSV PDB
7.5 -0.8051 3.8056 CSV PDB
8.0 -0.7679 3.8055 CSV PDB
8.5 -0.7243 3.8053 CSV PDB
9.0 -0.6741 3.8048 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH