Concizumab / 4dtg

A4DAB071_4dtg_H_L | Source PDB: 4dtg | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7296
Max Score
1.5107
Min Score
-3.7431
Total Score
-321.03
Score CDR
-0.4318
Score non-CDR
-0.7704

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVKPGGSLRLSCAASGFTFSNYAMSWVRQTPEKRLEWVATISRSGSYSYFPDSVQGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARLGGYDEGDAMDSWGQGTTVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESDIVMTQTPLSLSVTPGQPASISCKSSQSLLESDGKTYLNWYLQKPGQSPQLLIYLVSILDSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCLQATHFPQTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7431
Maximum Score 1.5107
Average Score -0.7296
Total Score -321.0293

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0543 Soluble
2 V H -1.3095 Soluble
3 Q H -1.4874 Soluble
4 L H 0.0000 Buried
5 V H 0.5345 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5173 Soluble
8 G H -1.1555 Soluble
9 G H -0.8136 Soluble
11 G H -0.6356 Soluble
12 L H -0.4436 Soluble
13 V H 0.0000 Buried
14 K H -2.1720 Soluble
15 P H -1.9828 Soluble
16 G H -1.5555 Soluble
17 G H -1.2789 Soluble
18 S H -1.2526 Soluble
19 L H -1.2786 Soluble
20 R H -2.2317 Soluble
21 L H 0.0000 Buried
22 S H -0.5261 Soluble
23 C H 0.0000 Buried
24 A H -0.1975 Soluble
25 A H 0.0000 Buried
26 S H -0.9324 Soluble
27 G H -1.1326 Soluble
28 F H -0.5856 Soluble
29 T H -0.4764 Soluble
30 F H 0.0000 Buried
35 S H -1.5692 Soluble
36 N H -1.6518 Soluble
37 Y H -0.9796 Soluble
38 A H -0.8768 Soluble
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H 0.0000 Buried
45 T H -2.2481 Soluble
46 P H -2.2326 Soluble
47 E H -3.3593 Soluble
48 K H -3.6180 Soluble
49 R H -3.4943 Soluble
50 L H 0.0000 Buried
51 E H -1.1244 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 T H 0.0000 Buried
56 I H 0.0000 Buried
57 S H -0.6260 Soluble
58 R H -2.1986 Soluble
59 S H -1.3663 Soluble
62 G H -0.8854 Soluble
63 S H -0.1381 Soluble
64 Y H 0.9581 Aggregation-prone
65 S H 0.7300 Aggregation-prone
66 Y H 1.1148 Aggregation-prone
67 F H -0.0798 Soluble
68 P H 0.0000 Buried
69 D H -2.2126 Soluble
70 S H -1.4083 Soluble
71 V H 0.0000 Buried
72 Q H -1.9465 Soluble
74 G H -1.4697 Soluble
75 R H -1.2831 Soluble
76 F H 0.0000 Buried
77 T H -0.7461 Soluble
78 I H 0.0000 Buried
79 S H -0.4165 Soluble
80 R H -1.2413 Soluble
81 D H -1.4448 Soluble
82 N H -1.9608 Soluble
83 A H -1.3034 Soluble
84 K H -2.2069 Soluble
85 N H -1.6285 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6844 Soluble
89 L H 0.0000 Buried
90 Q H -1.5994 Soluble
91 M H 0.0000 Buried
92 N H -1.3320 Soluble
93 S H -1.2498 Soluble
94 L H 0.0000 Buried
95 R H -2.4282 Soluble
96 A H -1.8218 Soluble
97 E H -2.2689 Soluble
98 D H 0.0000 Buried
99 T H -0.7461 Soluble
100 A H 0.0000 Buried
101 V H -0.0339 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.3118 Soluble
107 L H -0.3325 Soluble
108 G H 0.0000 Buried
109 G H -0.5735 Soluble
110 Y H -0.0221 Soluble
111 D H -1.5916 Soluble
112A E H -1.0600 Soluble
112 G H 0.0000 Buried
113 D H -0.8793 Soluble
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.6148 Soluble
117 S H -0.9556 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.4456 Soluble
121 G H -0.5695 Soluble
122 T H -0.2250 Soluble
123 T H -0.2669 Soluble
124 V H 0.0000 Buried
125 T H -0.6379 Soluble
126 V H 0.0000 Buried
127 S H -1.1530 Soluble
128 S H -0.8154 Soluble
129 A H -0.6142 Soluble
130 S H -0.7422 Soluble
131 T H -0.9526 Soluble
132 K H -1.6753 Soluble
133 G H -1.6347 Soluble
134 P H -0.6958 Soluble
135 S H -0.4373 Soluble
136 V H 0.0000 Buried
137 F H -0.5768 Soluble
138 P H -0.9915 Soluble
139 L H 0.0000 Buried
140 A H -0.5214 Soluble
141 P H -0.3947 Soluble
142 C H -0.5240 Soluble
143 S H -0.8111 Soluble
144 R H -1.4371 Soluble
145 S H -1.2321 Soluble
146 T H -1.0970 Soluble
147 S H -1.2990 Soluble
148 E H -2.0683 Soluble
149 S H -1.1730 Soluble
150 T H -0.7566 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.5153 Soluble
160 Y H 0.0000 Buried
161 F H -0.3756 Soluble
162 P H 0.0000 Buried
163 E H -0.5186 Soluble
164 P H -0.6743 Soluble
165 V H -0.6419 Soluble
166 T H -0.5653 Soluble
167 V H -0.3382 Soluble
168 S H -0.4862 Soluble
169 W H 0.0000 Buried
170 N H -1.0696 Soluble
171 S H -0.8033 Soluble
172 G H -0.5723 Soluble
173 A H -0.2617 Soluble
174 L H -0.0608 Soluble
175 T H -0.1776 Soluble
176 S H -0.1724 Soluble
177 G H -0.1943 Soluble
178 V H 0.1490 Aggregation-prone
179 H H -0.2898 Soluble
180 T H -0.0330 Soluble
181 F H 0.0000 Buried
182 P H -0.3160 Soluble
183 A H 0.1039 Aggregation-prone
184 V H 0.3858 Aggregation-prone
185 L H 1.1511 Aggregation-prone
186 Q H 0.2385 Aggregation-prone
187 S H -0.1094 Soluble
188 S H -0.2590 Soluble
189 G H -0.0540 Soluble
190 L H 0.0690 Aggregation-prone
191 Y H 0.3886 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1052 Soluble
199 V H 0.0000 Buried
200 P H -0.5674 Soluble
201 S H -0.9483 Soluble
202 S H -0.8485 Soluble
203 S H 0.0000 Buried
204 L H -1.0035 Soluble
205 G H -1.1545 Soluble
206 T H -1.1073 Soluble
207 K H -2.0464 Soluble
208 T H -1.7685 Soluble
209 Y H 0.0000 Buried
210 T H -1.9653 Soluble
211 C H 0.0000 Buried
212 N H -1.6183 Soluble
213 V H 0.0000 Buried
214 D H -1.8253 Soluble
215 H H 0.0000 Buried
216 K H -2.7691 Soluble
217 P H -1.6435 Soluble
218 S H -1.8995 Soluble
219 N H -2.6376 Soluble
220 T H -2.0874 Soluble
221 K H -2.6324 Soluble
222 V H -1.6373 Soluble
223 D H -2.6709 Soluble
224 K H -2.3126 Soluble
225 R H -2.8778 Soluble
226 V H 0.0000 Buried
227 E H -2.6308 Soluble
228 S H -1.3244 Soluble
1 D L -1.0922 Soluble
2 I L 0.0818 Aggregation-prone
3 V L 0.8739 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.3042 Soluble
6 Q L -0.2403 Soluble
7 T L -0.1308 Soluble
8 P L 0.3943 Aggregation-prone
9 L L 1.0263 Aggregation-prone
10 S L 0.1258 Aggregation-prone
11 L L 0.1356 Aggregation-prone
12 S L -0.4738 Soluble
13 V L 0.0000 Buried
14 T L -1.3476 Soluble
15 P L -1.6709 Soluble
16 G L -1.8959 Soluble
17 Q L -1.9682 Soluble
18 P L -1.8906 Soluble
19 A L 0.0000 Buried
20 S L -0.6912 Soluble
21 I L 0.0000 Buried
22 S L -0.9039 Soluble
23 C L 0.0000 Buried
24 K L -2.0939 Soluble
25 S L 0.0000 Buried
26 S L -0.8733 Soluble
27 Q L -1.4982 Soluble
28 S L -1.0451 Soluble
29 L L 0.0000 Buried
30 L L -0.2337 Soluble
31 E L -1.0617 Soluble
32 S L -1.0704 Soluble
34 D L -1.5807 Soluble
35 G L -1.3126 Soluble
36 K L -1.1486 Soluble
37 T L 0.0000 Buried
38 Y L 0.0000 Buried
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 L L 0.0000 Buried
44 Q L -1.1240 Soluble
45 K L -1.8138 Soluble
46 P L -1.6445 Soluble
47 G L -1.6833 Soluble
48 Q L -2.0540 Soluble
49 S L -1.4299 Soluble
50 P L 0.0000 Buried
51 Q L -1.3128 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 L L -0.0003 Soluble
57 V L 0.2205 Aggregation-prone
65 S L 0.3644 Aggregation-prone
66 I L 1.1002 Aggregation-prone
67 L L 0.3532 Aggregation-prone
68 D L -0.2478 Soluble
69 S L -0.3328 Soluble
70 G L -0.7736 Soluble
71 V L -0.6781 Soluble
72 P L -1.1933 Soluble
74 D L -2.0168 Soluble
75 R L -2.1602 Soluble
76 F L 0.0000 Buried
77 S L -0.9192 Soluble
78 G L -0.3652 Soluble
79 S L -0.7339 Soluble
80 G L -1.1096 Soluble
83 S L -0.9063 Soluble
84 G L -0.9113 Soluble
85 T L -1.5732 Soluble
86 D L -2.3045 Soluble
87 F L 0.0000 Buried
88 T L -1.1352 Soluble
89 L L 0.0000 Buried
90 K L -1.8177 Soluble
91 I L 0.0000 Buried
92 S L -2.1607 Soluble
93 R L -2.9119 Soluble
94 V L 0.0000 Buried
95 E L -2.7286 Soluble
96 A L -2.4347 Soluble
97 E L -2.8580 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 G L -1.4505 Soluble
101 V L -0.6973 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 A L 0.0952 Aggregation-prone
108 T L -0.0949 Soluble
109 H L 0.1627 Aggregation-prone
114 F L 1.5107 Aggregation-prone
115 P L 0.4436 Aggregation-prone
116 Q L 0.3143 Aggregation-prone
117 T L 0.1905 Aggregation-prone
118 F L -0.0877 Soluble
119 G L 0.0000 Buried
120 G L -1.0341 Soluble
121 G L -0.8434 Soluble
122 T L 0.0000 Buried
123 K L -1.0171 Soluble
124 V L 0.0000 Buried
125 E L -1.1747 Soluble
126 I L 0.0000 Buried
127 K L -1.3544 Soluble
128 R L -1.0138 Soluble
129 T L -0.0814 Soluble
130 V L 0.3425 Aggregation-prone
131 A L 0.1140 Aggregation-prone
132 A L -0.1101 Soluble
133 P L 0.0000 Buried
134 S L -0.1302 Soluble
135 V L 0.0000 Buried
136 F L 0.1423 Aggregation-prone
137 I L 0.1238 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.2377 Soluble
140 P L 0.0000 Buried
141 S L -1.3421 Soluble
142 D L -2.6284 Soluble
143 E L -2.4993 Soluble
144 Q L 0.0000 Buried
145 L L -2.1043 Soluble
146 K L -2.6383 Soluble
147 S L -1.5786 Soluble
148 G L -1.2535 Soluble
149 T L -1.0927 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8431 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6670 Soluble
162 R L -2.8601 Soluble
163 E L -3.1782 Soluble
164 A L -2.2831 Soluble
165 K L -2.3524 Soluble
166 V L -0.9267 Soluble
167 Q L -0.4399 Soluble
168 W L 0.0000 Buried
169 K L -0.4141 Soluble
170 V L 0.0000 Buried
171 D L -1.8310 Soluble
172 N L -1.4331 Soluble
173 A L -0.2062 Soluble
174 L L 0.7188 Aggregation-prone
175 Q L -0.0883 Soluble
176 S L -0.4603 Soluble
177 G L -0.9011 Soluble
178 N L -0.8344 Soluble
179 S L -1.1517 Soluble
180 Q L -1.4565 Soluble
181 E L -1.9357 Soluble
182 S L -0.9515 Soluble
183 V L -0.5713 Soluble
184 T L -0.8934 Soluble
185 E L -2.1723 Soluble
186 Q L 0.0000 Buried
187 D L -2.2437 Soluble
188 S L -2.4837 Soluble
189 K L -2.8085 Soluble
190 D L -2.0526 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6909 Soluble
199 L L 0.0000 Buried
200 T L -0.2889 Soluble
201 L L -0.7181 Soluble
202 S L -1.1136 Soluble
203 K L -2.1064 Soluble
204 A L -1.9782 Soluble
205 D L -2.8132 Soluble
206 Y L 0.0000 Buried
207 E L -3.7247 Soluble
208 K L -3.7431 Soluble
209 H L -3.0141 Soluble
210 K L -3.1182 Soluble
211 V L -1.2934 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.5799 Soluble
216 V L 0.0000 Buried
217 T L -1.1685 Soluble
218 H L 0.0000 Buried
219 Q L -1.7526 Soluble
220 G L -0.4401 Soluble
221 L L -0.2363 Soluble
222 S L -0.4699 Soluble
223 S L -0.4304 Soluble
224 P L -0.5188 Soluble
225 V L 0.0816 Aggregation-prone
226 T L -0.3441 Soluble
227 K L -0.6023 Soluble
228 S L -0.4577 Soluble
229 F L 0.0000 Buried
230 N L -1.6591 Soluble
231 R L -2.3313 Soluble
232 G L -1.9282 Soluble
233 E L -2.1682 Soluble
234 C L -0.7506 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7547 4.0433 CSV PDB
4.5 -0.8073 3.975 CSV PDB
5.0 -0.8695 3.9031 CSV PDB
5.5 -0.93 3.8401 CSV PDB
6.0 -0.9761 3.7918 CSV PDB
6.5 -0.9984 3.7485 CSV PDB
7.0 -0.9959 3.6939 CSV PDB
7.5 -0.9761 3.625 CSV PDB
8.0 -0.9462 3.5508 CSV PDB
8.5 -0.9086 3.483 CSV PDB
9.0 -0.8634 3.4334 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH