Anvatabart / 8tjf

A4DAB021_8tjf_H_L | Source PDB: 8tjf | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.725
Max Score
2.0841
Min Score
-3.1952
Total Score
-308.86
Score CDR
-0.6158
Score non-CDR
-0.7386

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVCPLAPSGTADLGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKGQPKAAPSVRLFPPCSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.1952
Maximum Score 2.0841
Average Score -0.725
Total Score -308.8552

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1025 Soluble
2 V H -1.3344 Soluble
3 Q H -1.1081 Soluble
4 L H 0.0000 Buried
5 V H 0.7146 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4125 Soluble
8 G H -0.8527 Soluble
9 G H -0.0969 Soluble
11 G H 0.6049 Aggregation-prone
12 L H 0.8495 Aggregation-prone
13 V H -0.2884 Soluble
14 Q H -1.4578 Soluble
15 P H -1.6976 Soluble
16 G H -1.4047 Soluble
17 G H -1.0978 Soluble
18 S H -1.0564 Soluble
19 L H -1.0507 Soluble
20 R H -2.1253 Soluble
21 L H 0.0000 Buried
22 S H -0.4288 Soluble
23 C H 0.0000 Buried
24 A H -0.2135 Soluble
25 A H 0.0000 Buried
26 S H -1.0769 Soluble
27 G H -1.4941 Soluble
28 F H -1.5987 Soluble
29 N H -2.3538 Soluble
30 I H 0.0000 Buried
35 K H -2.9809 Soluble
36 D H -3.1222 Soluble
37 T H 0.0000 Buried
38 Y H -0.2491 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7295 Soluble
45 A H -1.1079 Soluble
46 P H -0.8381 Soluble
47 G H -1.4599 Soluble
48 K H -2.4435 Soluble
49 G H -1.7024 Soluble
50 L H 0.0000 Buried
51 E H -1.3344 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H -0.5122 Soluble
56 I H 0.0000 Buried
57 Y H -0.4685 Soluble
58 P H -1.2141 Soluble
59 T H -1.3141 Soluble
62 N H -0.9590 Soluble
63 G H -0.5102 Soluble
64 Y H 0.5437 Aggregation-prone
65 T H -0.4040 Soluble
66 R H -1.9057 Soluble
67 Y H -1.6486 Soluble
68 A H -1.7456 Soluble
69 D H -2.6140 Soluble
70 S H -1.6867 Soluble
71 V H 0.0000 Buried
72 K H -2.7126 Soluble
74 G H -1.6256 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -1.0199 Soluble
78 I H 0.0000 Buried
79 S H -0.1306 Soluble
80 A H -0.8119 Soluble
81 D H -1.5133 Soluble
82 T H -1.6324 Soluble
83 S H -1.3911 Soluble
84 K H -2.2091 Soluble
85 N H -2.0052 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5763 Soluble
89 L H 0.0000 Buried
90 Q H -1.2413 Soluble
91 M H 0.0000 Buried
92 N H -1.3530 Soluble
93 S H -1.2052 Soluble
94 L H 0.0000 Buried
95 R H -2.4089 Soluble
96 A H -1.7921 Soluble
97 E H -2.2706 Soluble
98 D H 0.0000 Buried
99 T H -0.5277 Soluble
100 A H 0.0000 Buried
101 V H 0.5625 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H -0.1732 Soluble
107 W H 0.0000 Buried
108 G H -0.5760 Soluble
109 G H -1.5068 Soluble
110 D H -2.1324 Soluble
111 G H -0.9969 Soluble
112 F H -0.1136 Soluble
113 Y H 0.4726 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.1039 Soluble
117 Y H -0.1553 Soluble
118 W H -0.4004 Soluble
119 G H 0.0000 Buried
120 Q H -1.4223 Soluble
121 G H -0.4404 Soluble
122 T H 0.4197 Aggregation-prone
123 L H 1.4416 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.1148 Aggregation-prone
126 V H 0.0000 Buried
127 S H -0.8900 Soluble
128 S H -0.8406 Soluble
129 A H -0.5539 Soluble
130 S H -0.6572 Soluble
131 T H -0.7044 Soluble
132 K H -1.1839 Soluble
133 G H -1.3935 Soluble
134 P H 0.0000 Buried
135 S H -0.6114 Soluble
136 V H 0.0000 Buried
137 C H 0.0000 Buried
138 P H -1.1810 Soluble
139 L H 0.0000 Buried
140 A H -1.2417 Soluble
141 P H 0.0000 Buried
142 S H -0.7869 Soluble
143 G H -0.6316 Soluble
144 T H -0.5042 Soluble
145 A H -0.3927 Soluble
146 D H -0.4062 Soluble
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H -0.4900 Soluble
153 D H -0.6233 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.6403 Soluble
157 E H -0.9770 Soluble
158 P H -0.9885 Soluble
159 V H -0.6993 Soluble
160 T H -0.5142 Soluble
161 V H -0.2920 Soluble
162 S H -0.4008 Soluble
163 W H 0.0000 Buried
164 N H -0.8745 Soluble
165 S H -0.6750 Soluble
166 G H -0.5337 Soluble
167 A H -0.2522 Soluble
168 L H 0.0306 Aggregation-prone
169 T H -0.1292 Soluble
170 S H -0.1469 Soluble
171 G H -0.1659 Soluble
172 V H 0.0820 Aggregation-prone
173 H H -0.4049 Soluble
174 T H -0.1938 Soluble
175 F H 0.0000 Buried
176 P H -0.2583 Soluble
177 A H 0.1868 Aggregation-prone
178 V H 0.0000 Buried
179 L H 1.1091 Aggregation-prone
180 Q H 0.2659 Aggregation-prone
181 S H -0.1139 Soluble
182 S H -0.1883 Soluble
183 G H 0.0345 Aggregation-prone
184 L H 0.0076 Aggregation-prone
185 Y H 0.2102 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.2711 Soluble
193 V H 0.0000 Buried
194 P H -0.6205 Soluble
195 S H -0.4838 Soluble
196 S H -0.5576 Soluble
197 S H -0.5793 Soluble
198 L H -0.8729 Soluble
199 G H -1.3514 Soluble
200 T H -1.0121 Soluble
201 Q H -1.4438 Soluble
202 T H -1.2732 Soluble
203 Y H 0.0000 Buried
204 I H -1.6027 Soluble
205 C H 0.0000 Buried
206 N H 0.0000 Buried
207 V H 0.0000 Buried
208 N H -1.7592 Soluble
209 H H 0.0000 Buried
210 K H -2.6640 Soluble
211 P H -1.6552 Soluble
212 S H -1.7750 Soluble
213 N H -2.4943 Soluble
214 T H -2.0210 Soluble
215 K H -2.5715 Soluble
216 V H -1.5511 Soluble
217 D H -2.6451 Soluble
218 K H -2.2991 Soluble
219 R H -2.8413 Soluble
220 V H 0.0000 Buried
221 E H -2.9219 Soluble
222 P H -1.8567 Soluble
223 K H -2.2265 Soluble
1 D L -2.2382 Soluble
2 I L 0.0000 Buried
3 Q L -2.2206 Soluble
4 M L 0.0000 Buried
5 T L -1.3728 Soluble
6 Q L 0.0000 Buried
7 S L -0.8991 Soluble
8 P L -0.6964 Soluble
9 S L -0.9617 Soluble
10 S L -1.0565 Soluble
11 L L -0.4990 Soluble
12 S L -0.7235 Soluble
13 A L 0.0000 Buried
14 S L -0.1569 Soluble
15 V L 0.6586 Aggregation-prone
16 G L -0.6337 Soluble
17 D L -1.5536 Soluble
18 R L -2.2382 Soluble
19 V L 0.0000 Buried
20 T L -0.6571 Soluble
21 I L 0.0000 Buried
22 T L -0.7557 Soluble
23 C L 0.0000 Buried
24 R L -2.6071 Soluble
25 A L 0.0000 Buried
26 S L -2.1571 Soluble
27 Q L -2.7409 Soluble
28 D L -2.8037 Soluble
29 V L 0.0000 Buried
36 N L -1.8821 Soluble
37 T L -1.0050 Soluble
38 A L -0.0600 Soluble
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.7613 Soluble
46 P L -1.2404 Soluble
47 G L -1.6723 Soluble
48 K L -2.6163 Soluble
49 A L -1.6420 Soluble
50 P L 0.0000 Buried
51 K L -1.4910 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.0161 Aggregation-prone
56 S L 0.6308 Aggregation-prone
57 A L 0.0000 Buried
65 S L 0.6317 Aggregation-prone
66 F L 2.0841 Aggregation-prone
67 L L 1.3175 Aggregation-prone
68 Y L 0.5440 Aggregation-prone
69 S L -0.0025 Soluble
70 G L -0.4852 Soluble
71 V L 0.0000 Buried
72 P L -0.3543 Soluble
74 S L -0.3993 Soluble
75 R L -0.8053 Soluble
76 F L 0.0000 Buried
77 S L 0.0463 Aggregation-prone
78 G L 0.0000 Buried
79 S L -0.6431 Soluble
80 R L -1.7329 Soluble
83 S L -1.2687 Soluble
84 G L -1.8444 Soluble
85 T L -2.2207 Soluble
86 D L -1.9104 Soluble
87 F L 0.0000 Buried
88 T L -0.7825 Soluble
89 L L 0.0000 Buried
90 T L -0.5974 Soluble
91 I L 0.0000 Buried
92 S L -1.3081 Soluble
93 S L -1.0708 Soluble
94 L L 0.0000 Buried
95 Q L -0.8939 Soluble
96 P L -0.8111 Soluble
97 E L -1.9027 Soluble
98 D L 0.0000 Buried
99 F L -0.5639 Soluble
100 A L -0.9385 Soluble
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.5744 Aggregation-prone
108 Y L 0.7751 Aggregation-prone
109 T L 0.2771 Aggregation-prone
114 T L -0.3781 Soluble
115 P L -0.8498 Soluble
116 P L 0.0000 Buried
117 T L -0.7017 Soluble
118 F L -0.5884 Soluble
119 G L 0.0000 Buried
120 Q L -2.0169 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.9880 Soluble
124 V L 0.0000 Buried
125 E L -1.1246 Soluble
126 I L 0.3488 Aggregation-prone
127 K L -1.2519 Soluble
128 G L -1.5819 Soluble
129 Q L -1.9299 Soluble
130 P L -1.8720 Soluble
131 K L -2.4981 Soluble
132 A L -1.5070 Soluble
133 A L -0.9408 Soluble
134 P L 0.0000 Buried
135 S L -0.3083 Soluble
136 V L 0.0000 Buried
137 R L -0.5242 Soluble
138 L L -0.4283 Soluble
139 F L 0.0000 Buried
140 P L -0.0778 Soluble
141 P L -0.4456 Soluble
142 C L 0.0000 Buried
143 S L -1.1183 Soluble
144 E L -1.6816 Soluble
145 E L -1.8238 Soluble
146 L L -1.8032 Soluble
147 Q L -2.0724 Soluble
148 A L -1.8209 Soluble
149 N L -2.7389 Soluble
150 K L -2.8593 Soluble
151 A L 0.0000 Buried
152 T L 0.0000 Buried
153 L L 0.0000 Buried
154 V L 0.0000 Buried
155 C L 0.0000 Buried
156 L L 0.0000 Buried
157 I L 0.0000 Buried
158 S L 0.0000 Buried
159 D L -1.6752 Soluble
160 F L 0.0000 Buried
161 Y L -1.6881 Soluble
162 P L -1.4531 Soluble
163 G L -1.0278 Soluble
164 A L -0.1010 Soluble
165 V L 0.1002 Aggregation-prone
166 T L 0.0216 Aggregation-prone
167 V L 0.2686 Aggregation-prone
168 A L -0.3475 Soluble
169 W L 0.0000 Buried
170 K L -1.0657 Soluble
171 A L 0.0000 Buried
172 D L -1.3350 Soluble
173 S L -0.9108 Soluble
174 S L -0.7888 Soluble
175 P L -0.8728 Soluble
176 V L -0.8557 Soluble
177 K L -1.6826 Soluble
178 A L -0.8599 Soluble
179 G L -0.7419 Soluble
180 V L -0.4100 Soluble
181 E L -0.3561 Soluble
182 T L 0.0349 Aggregation-prone
183 T L 0.0343 Aggregation-prone
184 T L -0.1573 Soluble
185 P L -0.5999 Soluble
186 S L -1.3546 Soluble
187 K L -2.8256 Soluble
188 Q L -2.4326 Soluble
189 S L -1.8547 Soluble
190 N L -2.5342 Soluble
191 N L -2.9355 Soluble
192 K L -2.7141 Soluble
193 Y L -1.7875 Soluble
194 A L 0.0000 Buried
195 A L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 Y L 0.0000 Buried
199 L L 0.0000 Buried
200 S L -0.6980 Soluble
201 L L 0.0000 Buried
202 T L -2.0383 Soluble
203 P L -2.5492 Soluble
204 E L -3.1952 Soluble
205 Q L -2.3514 Soluble
206 W L 0.0000 Buried
207 K L -3.1225 Soluble
208 S L -2.4081 Soluble
209 H L -2.3883 Soluble
210 R L -2.5153 Soluble
211 S L -1.4864 Soluble
212 Y L 0.0000 Buried
213 S L 0.0000 Buried
214 C L 0.0000 Buried
215 Q L -0.8470 Soluble
216 V L 0.0000 Buried
217 T L -0.5728 Soluble
218 H L 0.0000 Buried
219 E L -2.3113 Soluble
220 G L -1.4192 Soluble
221 S L -0.9473 Soluble
222 T L -0.7398 Soluble
223 V L -0.5560 Soluble
224 E L -1.7471 Soluble
225 K L -1.5321 Soluble
226 T L -0.8740 Soluble
227 V L -0.5793 Soluble
228 A L -1.2947 Soluble
229 P L -1.5187 Soluble
230 T L -1.6039 Soluble
231 E L -1.8547 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7375 3.5373 CSV PDB
4.5 -0.7779 3.5373 CSV PDB
5.0 -0.8242 3.5373 CSV PDB
5.5 -0.8668 3.5373 CSV PDB
6.0 -0.8944 3.5373 CSV PDB
6.5 -0.898 3.5373 CSV PDB
7.0 -0.8783 3.5373 CSV PDB
7.5 -0.8437 3.5373 CSV PDB
8.0 -0.8012 3.5372 CSV PDB
8.5 -0.7532 3.5371 CSV PDB
9.0 -0.6998 3.5369 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH