Anbenitamab / 5vsh

A4DAB016_5vsh_C_D | Source PDB: 5vsh | Chains: C, D | Done
92.92
Yes
10 Å
Yes
Avg A4D Score
-0.6422
Max Score
1.4949
Min Score
-3.7572
Total Score
-272.95
Score CDR
-0.2052
Score non-CDR
-0.6953

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFTDYTMDWVRYAPGKGLEWVADVNPNSGGSIYNQRFKGRFTLSVDRSKNTLYLQMNSLRAEDTAVYYCARNLGPSFYFDYWGRGTLVTVSSASTKGPSVFPLAPCGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQRKPGDAPKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYIYPYTFGQGTKVEIKRTVAAPSVFIFPPSKEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) C (Heavy), D (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7572
Maximum Score 1.4949
Average Score -0.6422
Total Score -272.9522

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E C -2.0834 Soluble
2 V C -1.1352 Soluble
3 Q C -1.3215 Soluble
4 L C 0.0000 Buried
5 V C 0.7871 Aggregation-prone
6 E C 0.0000 Buried
7 S C -0.3064 Soluble
8 G C -0.9594 Soluble
9 G C -0.6105 Soluble
11 G C -0.3476 Soluble
12 L C -0.1794 Soluble
13 V C 0.0000 Buried
14 Q C -1.7324 Soluble
15 P C -1.9030 Soluble
16 G C -1.5562 Soluble
17 G C 0.0000 Buried
18 S C -1.2993 Soluble
19 L C -1.1910 Soluble
20 R C -2.0729 Soluble
21 L C 0.0000 Buried
22 S C -0.3475 Soluble
23 C C 0.0000 Buried
24 A C -0.2432 Soluble
25 A C 0.0000 Buried
26 S C -1.0399 Soluble
27 G C -1.2213 Soluble
28 F C -0.6143 Soluble
29 T C -0.6084 Soluble
30 F C 0.0000 Buried
35 T C -1.8506 Soluble
36 D C -1.7350 Soluble
37 Y C -0.4113 Soluble
38 T C -0.2039 Soluble
39 M C 0.0000 Buried
40 D C 0.0000 Buried
41 W C 0.0000 Buried
42 V C 0.0000 Buried
43 R C 0.0000 Buried
44 Y C -0.2680 Soluble
45 A C 0.0000 Buried
46 P C -0.9730 Soluble
47 G C -1.5873 Soluble
48 K C -2.4682 Soluble
49 G C -1.4504 Soluble
50 L C 0.0000 Buried
51 E C -0.5887 Soluble
52 W C 0.0000 Buried
53 V C 0.0000 Buried
54 A C 0.0000 Buried
55 D C 0.0000 Buried
56 V C 0.0000 Buried
57 N C -1.0879 Soluble
58 P C 0.0000 Buried
59 N C -2.3013 Soluble
62 S C -1.3387 Soluble
63 G C -1.2678 Soluble
64 G C -0.4844 Soluble
65 S C 0.4228 Aggregation-prone
66 I C 1.2065 Aggregation-prone
67 Y C -0.2826 Soluble
68 N C 0.0000 Buried
69 Q C -2.5483 Soluble
70 R C -2.9838 Soluble
71 F C 0.0000 Buried
72 K C -2.7054 Soluble
74 G C -1.9350 Soluble
75 R C -1.8271 Soluble
76 F C 0.0000 Buried
77 T C -0.7701 Soluble
78 L C 0.0000 Buried
79 S C -0.2945 Soluble
80 V C -1.2002 Soluble
81 D C -2.2429 Soluble
82 R C -3.2089 Soluble
83 S C -2.2442 Soluble
84 K C -2.8644 Soluble
85 N C -2.3574 Soluble
86 T C 0.0000 Buried
87 L C 0.0000 Buried
88 Y C -0.5083 Soluble
89 L C 0.0000 Buried
90 Q C -1.2819 Soluble
91 M C 0.0000 Buried
92 N C -1.4479 Soluble
93 S C -1.3402 Soluble
94 L C 0.0000 Buried
95 R C -2.8976 Soluble
96 A C -1.9861 Soluble
97 E C -2.6012 Soluble
98 D C 0.0000 Buried
99 T C -0.7768 Soluble
100 A C 0.0000 Buried
101 V C 0.1967 Aggregation-prone
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 A C 0.0000 Buried
106 R C 0.0000 Buried
107 N C 0.0000 Buried
108 L C 1.1823 Aggregation-prone
109 G C 0.3442 Aggregation-prone
110 P C 0.4538 Aggregation-prone
112 S C 0.7476 Aggregation-prone
113 F C 0.9199 Aggregation-prone
114 Y C 0.6507 Aggregation-prone
115 F C 0.0000 Buried
116 D C 0.1108 Aggregation-prone
117 Y C 0.1957 Aggregation-prone
118 W C -0.2192 Soluble
119 G C 0.0000 Buried
120 R C -1.8668 Soluble
121 G C 0.0000 Buried
122 T C -0.1709 Soluble
123 L C 0.2614 Aggregation-prone
124 V C 0.0000 Buried
125 T C -0.5739 Soluble
126 V C 0.0000 Buried
127 S C -0.9008 Soluble
128 S C -0.7482 Soluble
129 A C -0.4827 Soluble
130 S C -0.5836 Soluble
131 T C -0.5989 Soluble
132 K C -1.1350 Soluble
133 G C -1.3267 Soluble
134 P C 0.0000 Buried
135 S C -0.3721 Soluble
136 V C 0.0000 Buried
137 F C 0.0000 Buried
138 P C -1.1613 Soluble
139 L C 0.0000 Buried
140 A C -0.3498 Soluble
141 P C 0.0346 Aggregation-prone
142 C C 0.2781 Aggregation-prone
143 G C -0.7802 Soluble
144 G C -0.8508 Soluble
145 T C -0.6041 Soluble
146 A C -0.3210 Soluble
147 A C 0.0000 Buried
148 L C 0.0000 Buried
149 G C 0.0000 Buried
150 C C 0.0000 Buried
151 L C 0.0000 Buried
152 V C 0.0000 Buried
153 K C 0.0000 Buried
154 D C -0.3326 Soluble
155 Y C 0.0000 Buried
156 F C 0.0000 Buried
157 P C 0.0000 Buried
158 E C -0.4081 Soluble
159 P C -0.6642 Soluble
160 V C -0.4329 Soluble
161 T C -0.5023 Soluble
162 V C -0.0588 Soluble
163 S C -0.3093 Soluble
164 W C 0.0000 Buried
165 N C -0.6557 Soluble
166 S C -0.5930 Soluble
167 G C -0.5595 Soluble
168 A C -0.2312 Soluble
169 L C 0.0256 Aggregation-prone
170 T C -0.1474 Soluble
171 S C -0.1472 Soluble
172 G C -0.1422 Soluble
173 V C 0.2045 Aggregation-prone
174 H C -0.2860 Soluble
175 T C 0.0324 Aggregation-prone
176 F C 0.0000 Buried
177 P C -0.3439 Soluble
178 A C 0.2755 Aggregation-prone
179 V C 0.6325 Aggregation-prone
180 L C 1.4949 Aggregation-prone
181 Q C 0.4715 Aggregation-prone
182 S C -0.0178 Soluble
183 S C -0.1758 Soluble
184 G C 0.1014 Aggregation-prone
185 L C 0.2379 Aggregation-prone
186 Y C 0.5675 Aggregation-prone
187 S C 0.0000 Buried
188 L C 0.0000 Buried
189 S C 0.0000 Buried
190 S C 0.0000 Buried
191 V C 0.0000 Buried
192 V C 0.0000 Buried
193 T C -0.1066 Soluble
194 V C 0.0000 Buried
195 P C -0.5045 Soluble
196 S C -0.5345 Soluble
197 S C -0.6315 Soluble
198 S C -0.6063 Soluble
199 L C -0.7463 Soluble
200 G C -0.9972 Soluble
201 T C -0.7717 Soluble
202 Q C -1.3249 Soluble
203 T C -1.2057 Soluble
204 Y C 0.0000 Buried
205 I C -1.1998 Soluble
206 C C 0.0000 Buried
207 N C -1.2925 Soluble
208 V C 0.0000 Buried
209 N C -1.8168 Soluble
210 H C 0.0000 Buried
211 K C -2.7383 Soluble
212 P C -1.6038 Soluble
213 S C -1.8120 Soluble
214 N C -2.5867 Soluble
215 T C -2.0301 Soluble
216 K C -2.6502 Soluble
217 V C -1.5562 Soluble
218 D C -1.9772 Soluble
219 K C -2.0204 Soluble
220 K C -2.4890 Soluble
221 V C 0.0000 Buried
222 E C -2.5124 Soluble
223 P C -1.1866 Soluble
1 D D -1.9923 Soluble
2 I D 0.0000 Buried
3 Q D -2.2717 Soluble
4 M D 0.0000 Buried
5 T D -1.4445 Soluble
6 Q D 0.0000 Buried
7 S D -0.7379 Soluble
8 P D -0.4387 Soluble
9 S D -0.7233 Soluble
10 S D -0.5832 Soluble
11 L D -0.4487 Soluble
12 S D -0.7693 Soluble
13 A D -1.0011 Soluble
14 S D -0.8362 Soluble
15 V D -0.0510 Soluble
16 G D -0.8159 Soluble
17 D D -1.8640 Soluble
18 R D -2.3048 Soluble
19 V D 0.0000 Buried
20 T D -0.6453 Soluble
21 I D 0.0000 Buried
22 T D -0.9095 Soluble
23 C D 0.0000 Buried
24 K D -2.8272 Soluble
25 A D 0.0000 Buried
26 S D -2.3032 Soluble
27 Q D -2.8170 Soluble
28 D D -2.7272 Soluble
29 V D 0.0000 Buried
36 S D -0.6615 Soluble
37 I D 0.3585 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 A D 0.0000 Buried
41 W D 0.0000 Buried
42 Y D 0.0000 Buried
43 Q D 0.0000 Buried
44 R D 0.0000 Buried
45 K D -1.8709 Soluble
46 P D -1.4171 Soluble
47 G D -1.4102 Soluble
48 D D -2.0798 Soluble
49 A D -1.3319 Soluble
50 P D 0.0000 Buried
51 K D -1.0680 Soluble
52 L D 0.0000 Buried
53 L D 0.0000 Buried
54 I D 0.0000 Buried
55 Y D 0.5119 Aggregation-prone
56 S D 0.4802 Aggregation-prone
57 A D 0.0000 Buried
65 S D 0.1867 Aggregation-prone
66 Y D 0.8760 Aggregation-prone
67 R D -0.3711 Soluble
68 Y D -0.1892 Soluble
69 T D -0.3108 Soluble
70 G D -0.6432 Soluble
71 V D 0.0000 Buried
72 P D -0.5642 Soluble
74 S D -0.6370 Soluble
75 R D -0.7309 Soluble
76 F D 0.0000 Buried
77 S D -0.3704 Soluble
78 G D -0.2283 Soluble
79 S D -0.4657 Soluble
80 G D -0.8920 Soluble
83 S D -0.9882 Soluble
84 G D -1.7086 Soluble
85 T D -2.3409 Soluble
86 D D -2.8211 Soluble
87 F D 0.0000 Buried
88 T D -0.8618 Soluble
89 L D 0.0000 Buried
90 T D -0.5944 Soluble
91 I D 0.0000 Buried
92 S D -1.3233 Soluble
93 S D -1.1086 Soluble
94 L D 0.0000 Buried
95 Q D -1.0600 Soluble
96 P D -1.7937 Soluble
97 E D -2.3391 Soluble
98 D D 0.0000 Buried
99 F D 0.0000 Buried
100 A D 0.0000 Buried
101 T D 0.0000 Buried
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 Q D 0.0000 Buried
106 Q D 0.0000 Buried
107 Y D 0.0000 Buried
108 Y D 1.4875 Aggregation-prone
109 I D 1.4448 Aggregation-prone
114 Y D 1.2128 Aggregation-prone
115 P D 0.0000 Buried
116 Y D 0.0000 Buried
117 T D -0.3081 Soluble
118 F D -0.3817 Soluble
119 G D 0.0000 Buried
120 Q D -1.7706 Soluble
121 G D 0.0000 Buried
122 T D 0.0000 Buried
123 K D -1.0335 Soluble
124 V D 0.0000 Buried
125 E D -1.2470 Soluble
126 I D 0.0000 Buried
127 K D -1.2487 Soluble
128 R D -0.7138 Soluble
129 T D 0.0166 Aggregation-prone
130 V D 0.4640 Aggregation-prone
131 A D 0.2146 Aggregation-prone
132 A D -0.0648 Soluble
133 P D 0.0000 Buried
134 S D -0.0928 Soluble
135 V D 0.0000 Buried
136 F D 0.0790 Aggregation-prone
137 I D 0.1064 Aggregation-prone
138 F D 0.0000 Buried
139 P D -0.5828 Soluble
140 P D 0.0000 Buried
141 S D -1.4214 Soluble
142 K D -2.6237 Soluble
143 E D -2.4139 Soluble
144 Q D 0.0000 Buried
145 L D 0.0000 Buried
146 K D -2.6781 Soluble
147 S D -1.6959 Soluble
148 G D -1.2680 Soluble
149 T D -0.8892 Soluble
150 A D 0.0000 Buried
151 S D 0.0000 Buried
152 V D 0.0000 Buried
153 V D 0.0000 Buried
154 C D 0.0000 Buried
155 L D 0.0000 Buried
156 L D 0.0000 Buried
157 N D 0.0000 Buried
158 N D -0.7958 Soluble
159 F D 0.0000 Buried
160 Y D 0.0000 Buried
161 P D -1.7125 Soluble
162 R D -3.0921 Soluble
163 E D -3.2654 Soluble
164 A D -2.3215 Soluble
165 K D -2.4692 Soluble
166 V D -1.0807 Soluble
167 Q D -0.4414 Soluble
168 W D 0.0000 Buried
169 K D -0.4496 Soluble
170 V D 0.0000 Buried
171 D D -1.7902 Soluble
172 N D -1.4286 Soluble
173 A D -0.1594 Soluble
174 L D 0.8292 Aggregation-prone
175 Q D -0.0256 Soluble
176 S D -0.3698 Soluble
177 G D -0.8276 Soluble
178 N D -0.7078 Soluble
179 S D -0.9620 Soluble
180 Q D -1.0653 Soluble
181 E D -1.9897 Soluble
182 S D 0.0000 Buried
183 V D -0.8367 Soluble
184 T D -1.1539 Soluble
185 E D -2.3064 Soluble
186 Q D 0.0000 Buried
187 D D -2.0953 Soluble
188 S D -2.4555 Soluble
189 K D -2.7227 Soluble
190 D D -1.9728 Soluble
191 S D 0.0000 Buried
192 T D 0.0000 Buried
193 Y D 0.0000 Buried
194 S D 0.0000 Buried
195 L D 0.0000 Buried
196 S D 0.0000 Buried
197 S D 0.0000 Buried
198 T D 0.0000 Buried
199 L D 0.0000 Buried
200 T D -0.3266 Soluble
201 L D -0.5764 Soluble
202 S D -0.9816 Soluble
203 K D -2.0588 Soluble
204 A D -1.8073 Soluble
205 D D -2.2184 Soluble
206 Y D 0.0000 Buried
207 E D -3.7572 Soluble
208 K D -3.6810 Soluble
209 H D -3.2345 Soluble
210 K D -3.7190 Soluble
211 V D -2.0124 Soluble
212 Y D 0.0000 Buried
213 A D 0.0000 Buried
214 C D 0.0000 Buried
215 E D -0.7857 Soluble
216 V D 0.0000 Buried
217 T D -1.1843 Soluble
218 H D 0.0000 Buried
219 Q D -1.5663 Soluble
220 G D -0.3509 Soluble
221 L D -0.2246 Soluble
222 S D -0.4728 Soluble
223 S D -0.4344 Soluble
224 P D -0.5486 Soluble
225 V D -0.1010 Soluble
226 T D -0.5703 Soluble
227 K D -0.8734 Soluble
228 S D -0.7637 Soluble
229 F D 0.0000 Buried
230 N D -2.6989 Soluble
231 R D -3.4336 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7006 2.7263 CSV PDB
4.5 -0.7367 2.7263 CSV PDB
5.0 -0.7794 2.7263 CSV PDB
5.5 -0.8201 2.7263 CSV PDB
6.0 -0.8484 2.7263 CSV PDB
6.5 -0.856 2.7263 CSV PDB
7.0 -0.8428 2.7263 CSV PDB
7.5 -0.8159 2.7263 CSV PDB
8.0 -0.7815 2.7262 CSV PDB
8.5 -0.7417 2.7259 CSV PDB
9.0 -0.6966 2.7251 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH