Imalumab / 6foe

A4DAB141_6foe_H_L | Source PDB: 6foe | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7092
Max Score
1.9895
Min Score
-3.6605
Total Score
-311.32
Score CDR
0.0532
Score non-CDR
-0.7962

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSIYSMNWVRQAPGKGLEWVSSIGSSGGTTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAGSQWLYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHDIQMTQSPSSLSASVGDRVTITCRSSQRIMTYLNWYQQKPGKAPKLLIFVASHSQSGVPSRFRGSGSETDFTLTISGLQPEDSATYYCQQSFWTPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6605
Maximum Score 1.9895
Average Score -0.7092
Total Score -311.3222

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9633 Soluble
2 V H -1.0058 Soluble
3 Q H -1.2677 Soluble
4 L H 0.0000 Buried
5 L H 0.6271 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4689 Soluble
8 G H -0.8848 Soluble
9 G H -0.8342 Soluble
11 G H -0.5402 Soluble
12 L H -0.3612 Soluble
13 V H 0.0000 Buried
14 Q H -1.7306 Soluble
15 P H -1.9939 Soluble
16 G H -1.7440 Soluble
17 G H -1.4025 Soluble
18 S H -1.3094 Soluble
19 L H -1.2922 Soluble
20 R H -2.0318 Soluble
21 L H 0.0000 Buried
22 S H -0.3385 Soluble
23 C H 0.0000 Buried
24 A H -0.2483 Soluble
25 A H 0.0000 Buried
26 S H -1.0066 Soluble
27 G H -1.1557 Soluble
28 F H -0.1202 Soluble
29 T H 0.5036 Aggregation-prone
30 F H 0.0000 Buried
35 S H 0.3397 Aggregation-prone
36 I H 1.9895 Aggregation-prone
37 Y H 1.0837 Aggregation-prone
38 S H 0.5123 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8559 Soluble
45 A H -1.2553 Soluble
46 P H -1.2107 Soluble
47 G H -1.4716 Soluble
48 K H -2.2766 Soluble
49 G H -1.4214 Soluble
50 L H 0.0000 Buried
51 E H -0.9600 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 S H 0.0000 Buried
56 I H 0.0000 Buried
57 G H -0.0337 Soluble
58 S H 0.3067 Aggregation-prone
59 S H -0.3795 Soluble
62 G H -0.8013 Soluble
63 G H -0.7579 Soluble
64 T H -0.3245 Soluble
65 T H 0.1076 Aggregation-prone
66 Y H 0.2285 Aggregation-prone
67 Y H -0.6176 Soluble
68 A H -1.5062 Soluble
69 D H -2.6589 Soluble
70 S H -1.8397 Soluble
71 V H 0.0000 Buried
72 K H -2.5611 Soluble
74 G H -1.8149 Soluble
75 R H -1.8218 Soluble
76 F H 0.0000 Buried
77 T H -0.7474 Soluble
78 I H 0.0000 Buried
79 S H -0.3618 Soluble
80 R H -0.9878 Soluble
81 D H -1.5921 Soluble
82 N H -1.4249 Soluble
83 S H -1.5840 Soluble
84 K H -2.4176 Soluble
85 N H -1.8108 Soluble
86 T H -1.0053 Soluble
87 L H 0.0000 Buried
88 Y H -0.3214 Soluble
89 L H 0.0000 Buried
90 Q H -1.0975 Soluble
91 M H 0.0000 Buried
92 N H -1.7554 Soluble
93 S H -1.6021 Soluble
94 L H 0.0000 Buried
95 R H -2.9303 Soluble
96 A H -1.9572 Soluble
97 E H -2.3536 Soluble
98 D H 0.0000 Buried
99 T H -0.9000 Soluble
100 A H 0.0000 Buried
101 V H 0.0542 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 G H 0.0000 Buried
107 S H 0.0000 Buried
108 Q H 0.8433 Aggregation-prone
109 W H 1.7081 Aggregation-prone
110 L H 1.6214 Aggregation-prone
113 Y H 1.3552 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.6268 Soluble
117 V H 0.0000 Buried
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.3452 Soluble
121 G H 0.0000 Buried
122 T H -0.1860 Soluble
123 T H -0.2966 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.8487 Soluble
128 S H -0.7196 Soluble
129 A H -0.4810 Soluble
130 S H -0.6187 Soluble
131 T H -0.8868 Soluble
132 K H -1.5371 Soluble
133 G H -1.5750 Soluble
134 P H 0.0000 Buried
135 S H -0.2856 Soluble
136 V H 0.0000 Buried
137 F H -0.4967 Soluble
138 P H 0.0000 Buried
139 L H 0.0000 Buried
140 A H -1.1074 Soluble
141 P H 0.0000 Buried
142 S H -0.8526 Soluble
143 S H -1.3578 Soluble
144 K H -1.9388 Soluble
145 S H -1.1069 Soluble
146 T H -0.9958 Soluble
147 S H -0.9652 Soluble
148 G H -0.8867 Soluble
149 G H -0.8493 Soluble
150 T H -0.6599 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.6027 Soluble
160 Y H 0.0000 Buried
161 F H -0.3658 Soluble
162 P H 0.0000 Buried
163 E H -0.7224 Soluble
164 P H -0.7615 Soluble
165 V H 0.0000 Buried
166 T H -0.6547 Soluble
167 V H -0.1429 Soluble
168 S H -0.2858 Soluble
169 W H 0.0000 Buried
170 N H -0.6432 Soluble
171 S H -0.5214 Soluble
172 G H -0.3689 Soluble
173 A H -0.1188 Soluble
174 L H 0.0639 Aggregation-prone
175 T H -0.1001 Soluble
176 S H -0.1241 Soluble
177 G H -0.1108 Soluble
178 V H 0.2598 Aggregation-prone
179 H H 0.0000 Buried
180 T H -0.0845 Soluble
181 F H 0.0000 Buried
182 P H -0.2841 Soluble
183 A H 0.1449 Aggregation-prone
184 V H 0.3480 Aggregation-prone
185 L H 0.8319 Aggregation-prone
186 Q H 0.1217 Aggregation-prone
187 S H -0.1566 Soluble
188 S H -0.3123 Soluble
189 G H -0.1033 Soluble
190 L H -0.0163 Soluble
191 Y H 0.2899 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.0823 Soluble
199 V H 0.0000 Buried
200 P H -0.4813 Soluble
201 S H 0.0000 Buried
202 S H -0.7016 Soluble
203 S H -0.5641 Soluble
204 L H -0.9701 Soluble
205 G H -0.9657 Soluble
206 T H -0.6605 Soluble
207 Q H -1.0757 Soluble
208 T H -1.1234 Soluble
209 Y H 0.0000 Buried
210 I H -1.2354 Soluble
211 C H 0.0000 Buried
212 N H -1.2565 Soluble
213 V H 0.0000 Buried
214 N H -1.8064 Soluble
215 H H 0.0000 Buried
216 K H -2.7403 Soluble
217 P H -1.6143 Soluble
218 S H -1.8649 Soluble
219 N H -2.5717 Soluble
220 T H -2.0976 Soluble
221 K H -2.5196 Soluble
222 V H -1.2201 Soluble
223 D H -1.9472 Soluble
224 K H -1.8349 Soluble
225 R H -2.6634 Soluble
226 V H 0.0000 Buried
227 E H -2.8980 Soluble
228 P H -2.3358 Soluble
229 K H -2.6689 Soluble
230 S H -1.8949 Soluble
231 C H -1.8652 Soluble
232 D H -3.0376 Soluble
233 K H -3.0402 Soluble
234 T H -2.0506 Soluble
235 H H -1.6882 Soluble
1 D L -2.3551 Soluble
2 I L 0.0000 Buried
3 Q L -2.0796 Soluble
4 M L 0.0000 Buried
5 T L -1.0371 Soluble
6 Q L -0.7637 Soluble
7 S L -0.7542 Soluble
8 P L -0.6742 Soluble
9 S L -0.9454 Soluble
10 S L -1.0717 Soluble
11 L L -0.6866 Soluble
12 S L -0.9163 Soluble
13 A L -1.2210 Soluble
14 S L -1.2367 Soluble
15 V L -0.4514 Soluble
16 G L -1.3602 Soluble
17 D L -2.6979 Soluble
18 R L -2.7795 Soluble
19 V L 0.0000 Buried
20 T L -0.6533 Soluble
21 I L 0.0000 Buried
22 T L -0.6345 Soluble
23 C L 0.0000 Buried
24 R L -2.0437 Soluble
25 S L 0.0000 Buried
26 S L -2.2269 Soluble
27 Q L -2.7260 Soluble
28 R L -2.5833 Soluble
29 I L 0.0000 Buried
36 M L -0.2830 Soluble
37 T L -0.0246 Soluble
38 Y L 0.7182 Aggregation-prone
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.8956 Soluble
44 Q L 0.0000 Buried
45 K L -2.0336 Soluble
46 P L -1.6062 Soluble
47 G L -1.8055 Soluble
48 K L -2.5167 Soluble
49 A L -1.6933 Soluble
50 P L 0.0000 Buried
51 K L -1.7921 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 F L 0.2619 Aggregation-prone
56 V L 0.4767 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.9697 Soluble
66 H L -1.1820 Soluble
67 S L -1.0436 Soluble
68 Q L -0.8462 Soluble
69 S L -0.6436 Soluble
70 G L -0.5845 Soluble
71 V L -0.6036 Soluble
72 P L -0.5834 Soluble
74 S L -0.8106 Soluble
75 R L -1.1209 Soluble
76 F L 0.0000 Buried
77 R L -1.8600 Soluble
78 G L -1.1373 Soluble
79 S L -1.0173 Soluble
80 G L -0.8436 Soluble
83 S L -1.0190 Soluble
84 E L -1.7836 Soluble
85 T L -2.0300 Soluble
86 D L -1.9509 Soluble
87 F L 0.0000 Buried
88 T L -0.8249 Soluble
89 L L 0.0000 Buried
90 T L -1.0405 Soluble
91 I L 0.0000 Buried
92 S L -1.7420 Soluble
93 G L -1.4488 Soluble
94 L L 0.0000 Buried
95 Q L -1.0187 Soluble
96 P L -1.5389 Soluble
97 E L -1.9147 Soluble
98 D L 0.0000 Buried
99 S L 0.0000 Buried
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L 0.0000 Buried
108 F L 1.3672 Aggregation-prone
109 W L 0.5296 Aggregation-prone
114 T L 0.3314 Aggregation-prone
115 P L -0.3175 Soluble
116 L L 0.1308 Aggregation-prone
117 T L -0.4264 Soluble
118 F L -0.3202 Soluble
119 G L 0.0000 Buried
120 G L -1.2409 Soluble
121 G L -1.0761 Soluble
122 T L 0.0000 Buried
123 K L -2.0475 Soluble
124 V L 0.0000 Buried
125 E L -1.6172 Soluble
126 I L 0.0000 Buried
127 K L -1.1496 Soluble
128 R L -0.5985 Soluble
129 T L 0.2857 Aggregation-prone
130 V L 1.0878 Aggregation-prone
131 A L 0.3183 Aggregation-prone
132 A L 0.0722 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.0823 Soluble
135 V L 0.0000 Buried
136 F L -0.0196 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.4959 Soluble
140 P L -0.7556 Soluble
141 S L -1.3546 Soluble
142 D L -2.5936 Soluble
143 E L -2.2932 Soluble
144 Q L 0.0000 Buried
145 L L -2.0963 Soluble
146 K L -2.6340 Soluble
147 S L -1.6408 Soluble
148 G L -1.3378 Soluble
149 T L -0.9469 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7246 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3065 Soluble
162 R L -2.4935 Soluble
163 E L -3.0152 Soluble
164 A L -2.2344 Soluble
165 K L -2.2372 Soluble
166 V L -1.0267 Soluble
167 Q L -0.4291 Soluble
168 W L 0.0000 Buried
169 K L -0.5200 Soluble
170 V L 0.0000 Buried
171 D L -1.9547 Soluble
172 N L -1.4941 Soluble
173 A L -0.2659 Soluble
174 L L 0.7192 Aggregation-prone
175 Q L -0.3144 Soluble
176 S L -0.6351 Soluble
177 G L -1.2006 Soluble
178 N L -1.4210 Soluble
179 S L -1.3812 Soluble
180 Q L -1.5263 Soluble
181 E L -1.7676 Soluble
182 S L -0.7010 Soluble
183 V L -0.4304 Soluble
184 T L -0.9458 Soluble
185 E L -2.1993 Soluble
186 Q L 0.0000 Buried
187 D L -2.0957 Soluble
188 S L -2.2372 Soluble
189 K L -2.5150 Soluble
190 D L -1.7555 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7524 Soluble
199 L L 0.0000 Buried
200 T L -0.5766 Soluble
201 L L -0.7480 Soluble
202 S L -1.0509 Soluble
203 K L -2.1045 Soluble
204 A L -1.9003 Soluble
205 D L -2.4976 Soluble
206 Y L 0.0000 Buried
207 E L -3.6605 Soluble
208 K L -3.6294 Soluble
209 H L -3.1174 Soluble
210 K L -3.1197 Soluble
211 V L -1.5787 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.5212 Soluble
216 V L 0.0000 Buried
217 T L -1.0631 Soluble
218 H L 0.0000 Buried
219 Q L -1.3995 Soluble
220 G L -0.2433 Soluble
221 L L -0.1418 Soluble
222 S L -0.4257 Soluble
223 S L -0.3196 Soluble
224 P L -0.3102 Soluble
225 V L 0.3954 Aggregation-prone
226 T L -0.2558 Soluble
227 K L -0.6039 Soluble
228 S L -0.6030 Soluble
229 F L -0.7579 Soluble
230 N L -1.6686 Soluble
231 R L -2.1837 Soluble
232 G L -2.3399 Soluble
233 E L -2.6156 Soluble
234 C L -1.4907 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7787 4.2203 CSV PDB
4.5 -0.8219 4.2203 CSV PDB
5.0 -0.8725 4.2203 CSV PDB
5.5 -0.9202 4.2203 CSV PDB
6.0 -0.953 4.2203 CSV PDB
6.5 -0.9617 4.2203 CSV PDB
7.0 -0.9466 4.2203 CSV PDB
7.5 -0.9166 4.2203 CSV PDB
8.0 -0.8789 4.2202 CSV PDB
8.5 -0.8356 4.22 CSV PDB
9.0 -0.7863 4.2195 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH