Tomuzotuximab / 5t1m

A4DAB272_5t1m_D_C | Source PDB: 5t1m | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6312
Max Score
2.3623
Min Score
-3.8235
Total Score
-268.87
Score CDR
-0.3064
Score non-CDR
-0.6705

Calculation Parameters

Chain Sequence(s) VQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGA input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8235
Maximum Score 2.3623
Average Score -0.6312
Total Score -268.8726

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V D 0.3100 Aggregation-prone
3 Q D -1.2951 Soluble
4 L D 0.0000 Buried
5 K D -2.4095 Soluble
6 Q D 0.0000 Buried
7 S D -0.7929 Soluble
8 G D -0.9994 Soluble
9 P D -0.4737 Soluble
11 G D -0.3711 Soluble
12 L D -0.0043 Soluble
13 V D 0.0000 Buried
14 Q D -1.3750 Soluble
15 P D -1.5894 Soluble
16 S D -1.5600 Soluble
17 Q D -1.9720 Soluble
18 S D -1.5178 Soluble
19 L D 0.0000 Buried
20 S D -0.5189 Soluble
21 I D 0.0000 Buried
22 T D -0.4858 Soluble
23 C D 0.0000 Buried
24 T D -1.2682 Soluble
25 V D 0.0000 Buried
26 S D -0.9510 Soluble
27 G D -0.3347 Soluble
28 F D -0.2453 Soluble
29 S D -0.8749 Soluble
30 L D 0.0000 Buried
35 T D -1.0404 Soluble
36 N D -0.8674 Soluble
37 Y D 0.5026 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.5835 Soluble
45 S D -1.0650 Soluble
46 P D -1.1971 Soluble
47 G D -1.7592 Soluble
48 K D -2.2794 Soluble
49 G D -1.2708 Soluble
50 L D 0.0000 Buried
51 E D -0.6230 Soluble
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.3779 Soluble
58 S D -0.6273 Soluble
59 G D -1.2612 Soluble
63 G D -1.6047 Soluble
64 N D -1.8480 Soluble
65 T D -1.3197 Soluble
66 D D -1.1024 Soluble
67 Y D -0.4666 Soluble
68 N D -0.2673 Soluble
69 T D -0.2729 Soluble
70 P D -0.4090 Soluble
71 F D 0.0000 Buried
72 T D -0.5021 Soluble
74 S D -0.5585 Soluble
75 R D -0.9046 Soluble
76 L D 0.0000 Buried
77 S D -0.8414 Soluble
78 I D 0.0000 Buried
79 N D -1.8319 Soluble
80 K D -1.8147 Soluble
81 D D -2.3745 Soluble
82 N D -2.8071 Soluble
83 S D -2.0067 Soluble
84 K D -2.6149 Soluble
85 S D -1.8509 Soluble
86 Q D -1.7263 Soluble
87 V D 0.0000 Buried
88 F D -0.2854 Soluble
89 F D 0.0000 Buried
90 K D -1.1594 Soluble
92 N D -1.4875 Soluble
93 S D -1.4578 Soluble
94 L D 0.0000 Buried
95 Q D -2.1967 Soluble
96 S D -1.5689 Soluble
97 N D -2.1017 Soluble
98 D D 0.0000 Buried
99 T D -0.6941 Soluble
100 A D 0.0000 Buried
101 I D 0.3784 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0256 Aggregation-prone
109 T D 1.1423 Aggregation-prone
110 Y D 1.8263 Aggregation-prone
111 Y D 1.9375 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.0000 Buried
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.2936 Aggregation-prone
117 Y D 0.2946 Aggregation-prone
118 W D -0.4745 Soluble
119 G D 0.0000 Buried
120 Q D -1.8704 Soluble
121 G D -0.9384 Soluble
122 T D 0.0000 Buried
123 L D 0.1857 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3550 Soluble
126 V D 0.0000 Buried
127 S D -0.4936 Soluble
128 A D -0.2692 Soluble
129 A D -0.2540 Soluble
130 S D -0.4779 Soluble
131 T D -0.7490 Soluble
132 K D -1.2565 Soluble
133 G D -1.4597 Soluble
134 P D -0.6812 Soluble
135 S D -0.3756 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.0677 Soluble
139 L D 0.0000 Buried
140 A D -0.8432 Soluble
141 P D -0.6405 Soluble
142 S D -0.7443 Soluble
143 S D -0.7313 Soluble
144 G D -0.9447 Soluble
145 G D -0.9959 Soluble
146 T D -0.7095 Soluble
147 A D 0.0000 Buried
148 A D 0.0000 Buried
149 L D 0.0000 Buried
150 G D 0.0000 Buried
151 C D 0.0000 Buried
152 L D 0.0000 Buried
153 V D 0.0000 Buried
154 K D -0.2849 Soluble
155 D D -0.4731 Soluble
156 Y D 0.0000 Buried
157 F D 0.0000 Buried
158 P D 0.0000 Buried
159 E D -0.3810 Soluble
160 P D -0.6496 Soluble
161 V D -0.7294 Soluble
162 T D -0.6788 Soluble
163 V D -0.2736 Soluble
164 S D -0.4329 Soluble
165 W D 0.0000 Buried
166 N D -0.7981 Soluble
167 S D -0.6624 Soluble
168 G D -0.5333 Soluble
169 A D -0.2185 Soluble
170 L D 0.0156 Aggregation-prone
171 T D -0.1666 Soluble
172 S D -0.1929 Soluble
173 G D -0.2381 Soluble
174 V D 0.1234 Aggregation-prone
175 H D -0.1961 Soluble
176 T D 0.0120 Aggregation-prone
177 F D 0.0000 Buried
178 P D -0.1098 Soluble
179 A D 0.2801 Aggregation-prone
180 V D 0.6444 Aggregation-prone
181 L D 1.1567 Aggregation-prone
182 Q D 0.2849 Aggregation-prone
183 S D -0.0511 Soluble
184 S D -0.2029 Soluble
185 G D 0.0734 Aggregation-prone
186 L D 0.1219 Aggregation-prone
187 Y D 0.4017 Aggregation-prone
188 S D 0.0000 Buried
189 L D 0.0000 Buried
190 S D 0.0000 Buried
191 S D 0.0000 Buried
192 V D 0.0000 Buried
193 V D 0.0000 Buried
194 T D -0.1374 Soluble
195 V D 0.0000 Buried
196 P D -0.5647 Soluble
197 S D -0.6204 Soluble
198 S D -0.5764 Soluble
199 S D -0.4975 Soluble
200 L D -0.6559 Soluble
201 G D -0.8239 Soluble
202 T D -0.5997 Soluble
203 Q D -0.9024 Soluble
204 T D -0.9922 Soluble
205 Y D 0.0000 Buried
206 I D -1.3105 Soluble
207 C D 0.0000 Buried
208 N D -1.5231 Soluble
209 V D 0.0000 Buried
210 N D -2.1967 Soluble
211 H D 0.0000 Buried
212 K D -2.8766 Soluble
213 P D -1.6416 Soluble
214 S D -1.8892 Soluble
215 N D -2.6388 Soluble
216 T D -2.1291 Soluble
217 K D -2.7431 Soluble
218 V D -1.6843 Soluble
219 D D -2.5332 Soluble
220 K D -1.9689 Soluble
221 R D -2.5250 Soluble
222 V D 0.0000 Buried
223 E D -2.2843 Soluble
224 P D -1.6124 Soluble
225 K D -2.1111 Soluble
1 D C -1.4288 Soluble
2 I C 0.0000 Buried
3 L C 0.7678 Aggregation-prone
4 L C 0.0000 Buried
5 T C 0.0571 Aggregation-prone
6 Q C 0.0000 Buried
7 S C 0.5997 Aggregation-prone
8 P C 1.1643 Aggregation-prone
9 V C 2.3623 Aggregation-prone
10 I C 2.2028 Aggregation-prone
11 L C 1.0126 Aggregation-prone
12 S C -0.1804 Soluble
13 V C 0.0000 Buried
14 S C -1.8927 Soluble
15 P C -2.1616 Soluble
16 G C -2.3833 Soluble
17 E C -3.2339 Soluble
18 R C -3.4486 Soluble
19 V C 0.0000 Buried
20 S C -0.8150 Soluble
21 F C 0.0000 Buried
22 S C -0.5120 Soluble
23 C C 0.0000 Buried
24 R C -1.4634 Soluble
25 A C 0.0000 Buried
26 S C -0.7342 Soluble
27 Q C -1.3555 Soluble
28 S C -1.4101 Soluble
29 I C 0.0000 Buried
36 G C -1.0609 Soluble
37 T C -0.8460 Soluble
38 N C -0.8674 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C 0.0000 Buried
45 R C -1.6769 Soluble
46 T C -1.4779 Soluble
47 N C -1.8025 Soluble
48 G C -1.5357 Soluble
49 S C -0.9918 Soluble
50 P C 0.0000 Buried
51 R C -0.8871 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7141 Soluble
56 Y C -0.5468 Soluble
57 A C 0.0000 Buried
65 S C -1.1672 Soluble
66 E C -1.7918 Soluble
67 S C -1.0828 Soluble
68 I C -0.4853 Soluble
69 S C -0.5000 Soluble
70 G C -0.4701 Soluble
71 I C -0.2681 Soluble
72 P C -0.3128 Soluble
74 S C -0.5785 Soluble
75 R C -1.1589 Soluble
76 F C 0.0000 Buried
77 S C -0.8688 Soluble
78 G C -0.7407 Soluble
79 S C -0.9141 Soluble
80 G C -1.0902 Soluble
83 S C -1.0522 Soluble
84 G C -1.2512 Soluble
85 T C -1.7262 Soluble
86 D C -2.4551 Soluble
87 F C 0.0000 Buried
88 T C -0.8198 Soluble
89 L C 0.0000 Buried
90 S C -0.9582 Soluble
91 I C 0.0000 Buried
92 N C -2.5586 Soluble
93 S C -2.4672 Soluble
94 V C 0.0000 Buried
95 E C -2.6129 Soluble
96 S C -2.0153 Soluble
98 D C -1.1474 Soluble
99 I C 0.0000 Buried
100 A C 0.0000 Buried
101 D C -0.4930 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.7281 Soluble
108 N C -1.3398 Soluble
109 N C -1.0706 Soluble
114 W C 0.0462 Aggregation-prone
115 P C -0.2781 Soluble
116 T C 0.0000 Buried
117 T C -0.0913 Soluble
118 F C 0.1645 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0992 Aggregation-prone
121 G C 0.3336 Aggregation-prone
122 T C 0.0000 Buried
123 K C 0.2938 Aggregation-prone
124 L C 0.0000 Buried
125 E C -0.5582 Soluble
126 L C 0.0000 Buried
127 K C -1.3683 Soluble
128 R C -1.0813 Soluble
129 T C 0.1754 Aggregation-prone
130 V C 1.1006 Aggregation-prone
131 A C 0.3650 Aggregation-prone
132 A C 0.0590 Aggregation-prone
133 P C 0.0000 Buried
134 S C -0.1833 Soluble
135 V C 0.0000 Buried
136 F C 0.1350 Aggregation-prone
137 I C 0.0796 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5300 Soluble
140 P C -0.7198 Soluble
141 S C 0.0000 Buried
142 D C -2.5123 Soluble
143 E C -2.1043 Soluble
144 Q C 0.0000 Buried
145 L C -1.9842 Soluble
146 K C -2.4659 Soluble
147 S C -1.5122 Soluble
148 G C -0.9775 Soluble
149 T C -0.8057 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.7794 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.1179 Soluble
162 R C -1.8384 Soluble
163 E C -2.8457 Soluble
164 A C -2.1877 Soluble
165 K C -2.5137 Soluble
166 V C 0.0000 Buried
167 Q C -0.8041 Soluble
168 W C 0.0000 Buried
169 K C -0.7689 Soluble
170 V C 0.0000 Buried
171 D C -2.0480 Soluble
172 N C -1.6363 Soluble
173 A C -0.3991 Soluble
174 L C 0.4416 Aggregation-prone
175 Q C -0.2332 Soluble
176 S C -0.4401 Soluble
177 G C -0.8658 Soluble
178 N C -0.7738 Soluble
179 S C -1.0253 Soluble
180 Q C -1.1445 Soluble
181 E C -1.6575 Soluble
182 S C -0.5087 Soluble
183 V C -0.1301 Soluble
184 T C -0.4527 Soluble
185 E C -1.0981 Soluble
186 Q C 0.0000 Buried
187 D C -1.6867 Soluble
188 S C -1.9220 Soluble
189 K C -2.5671 Soluble
190 D C -2.0430 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.5981 Soluble
199 L C 0.0000 Buried
200 T C -0.2981 Soluble
201 L C -0.6932 Soluble
202 S C -1.2133 Soluble
203 K C -2.0215 Soluble
204 A C -2.0037 Soluble
205 D C -2.6863 Soluble
206 Y C 0.0000 Buried
207 E C -3.7603 Soluble
208 K C -3.8235 Soluble
209 H C -3.1875 Soluble
210 K C -3.1775 Soluble
211 V C -1.4816 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9624 Soluble
216 V C 0.0000 Buried
217 T C -1.2747 Soluble
218 H C 0.0000 Buried
219 Q C -1.5575 Soluble
220 G C -0.2284 Soluble
221 L C -0.1239 Soluble
222 S C -0.4388 Soluble
223 S C -0.4374 Soluble
224 P C -0.5990 Soluble
225 V C -0.1216 Soluble
226 T C -0.5659 Soluble
227 K C -0.7104 Soluble
228 S C -0.6099 Soluble
229 F C -0.7018 Soluble
230 N C -1.6317 Soluble
231 R C -2.3227 Soluble
232 G C -1.4210 Soluble
233 A C -0.7695 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6194 2.8127 CSV PDB
4.5 -0.6529 2.8057 CSV PDB
5.0 -0.6923 2.798 CSV PDB
5.5 -0.7297 2.7916 CSV PDB
6.0 -0.756 2.7903 CSV PDB
6.5 -0.7639 2.7989 CSV PDB
7.0 -0.7535 2.8191 CSV PDB
7.5 -0.7303 2.8474 CSV PDB
8.0 -0.6998 2.8795 CSV PDB
8.5 -0.6639 2.9135 CSV PDB
9.0 -0.6228 2.9495 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH