Ravagalimab / 6pe7

A4DAB219_6pe7_H_L | Source PDB: 6pe7 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7516
Max Score
1.0261
Min Score
-3.4569
Total Score
-321.69
Score CDR
-0.5856
Score non-CDR
-0.7731

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVKPGGSLRLSCAASGFTFSDYGMNWVRQAPGKGLEWIAYISSGRGNIYYADTVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARSWGYFDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIVMTQSPDSLAVSLGERATINCKSSQSLLNRGNQKNYLTWFQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISLQAEDVAVYYCQNDYTYPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4569
Maximum Score 1.0261
Average Score -0.7516
Total Score -321.688

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.8445 Soluble
2 V H -0.7235 Soluble
3 Q H -1.0404 Soluble
4 L H 0.0000 Buried
5 V H 0.7144 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5007 Soluble
8 G H -0.9651 Soluble
9 G H -0.5627 Soluble
11 G H -0.0236 Soluble
12 L H -0.0192 Soluble
13 V H -0.7883 Soluble
14 K H -2.0941 Soluble
15 P H -2.0583 Soluble
16 G H -1.6435 Soluble
17 G H -1.2980 Soluble
18 S H -1.2930 Soluble
19 L H -1.1096 Soluble
20 R H -2.0149 Soluble
21 L H 0.0000 Buried
22 S H -0.4283 Soluble
23 C H 0.0000 Buried
24 A H -0.2891 Soluble
25 A H 0.0000 Buried
26 S H -0.9460 Soluble
27 G H -1.0277 Soluble
28 F H -0.5880 Soluble
29 T H -0.6733 Soluble
30 F H 0.0000 Buried
35 S H -1.4303 Soluble
36 D H -1.7049 Soluble
37 Y H -0.5049 Soluble
38 G H 0.0000 Buried
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8471 Soluble
45 A H -1.2345 Soluble
46 P H -1.0219 Soluble
47 G H -1.4980 Soluble
48 K H -2.3266 Soluble
49 G H -1.4839 Soluble
50 L H 0.0000 Buried
51 E H -0.9110 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 A H 0.0000 Buried
55 Y H 0.6042 Aggregation-prone
56 I H 0.0000 Buried
57 S H -0.5723 Soluble
58 S H -1.4867 Soluble
59 G H -1.8059 Soluble
62 R H -2.1234 Soluble
63 G H -1.0544 Soluble
64 N H -0.4265 Soluble
65 I H 0.8560 Aggregation-prone
66 Y H 0.6559 Aggregation-prone
67 Y H -0.3220 Soluble
68 A H 0.0000 Buried
69 D H -2.3932 Soluble
70 T H -1.6841 Soluble
71 V H 0.0000 Buried
72 K H -2.4645 Soluble
74 G H -1.6579 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.5805 Soluble
78 I H 0.0000 Buried
79 S H -0.2606 Soluble
80 R H -1.0655 Soluble
81 D H -1.6567 Soluble
82 N H -2.1573 Soluble
83 A H -1.5460 Soluble
84 K H -2.4198 Soluble
85 N H -1.8969 Soluble
86 S H -1.1620 Soluble
87 L H 0.0000 Buried
88 Y H -0.4746 Soluble
89 L H 0.0000 Buried
90 Q H -1.0640 Soluble
91 M H 0.0000 Buried
92 N H -1.3886 Soluble
93 S H -1.3295 Soluble
94 L H 0.0000 Buried
95 R H -2.5836 Soluble
96 A H -1.8906 Soluble
97 E H -2.2991 Soluble
98 D H 0.0000 Buried
99 T H -0.8037 Soluble
100 A H 0.0000 Buried
101 V H -0.0930 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.1344 Aggregation-prone
107 S H 0.0000 Buried
108 W H 0.8759 Aggregation-prone
109 G H 0.4689 Aggregation-prone
114 Y H 0.4518 Aggregation-prone
115 F H 0.0000 Buried
116 D H -0.3056 Soluble
117 V H -0.0677 Soluble
118 W H -0.4446 Soluble
119 G H 0.0000 Buried
120 Q H -1.3644 Soluble
121 G H -0.6324 Soluble
122 T H -0.2705 Soluble
123 T H -0.2900 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1579 Soluble
128 S H -0.8770 Soluble
129 A H -0.5360 Soluble
130 S H -0.6434 Soluble
131 T H -0.7025 Soluble
132 K H -1.4253 Soluble
133 G H -1.5450 Soluble
134 P H -0.7371 Soluble
135 S H -0.4820 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0924 Soluble
139 L H 0.0000 Buried
140 A H -0.7748 Soluble
141 P H 0.0000 Buried
142 S H -0.4137 Soluble
143 G H -0.6511 Soluble
144 T H -0.4634 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.5999 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.5804 Soluble
158 P H -0.8639 Soluble
159 V H -0.7557 Soluble
160 T H -0.5990 Soluble
161 V H -0.2345 Soluble
162 S H -0.4297 Soluble
163 W H 0.0000 Buried
164 N H -0.8380 Soluble
165 S H -0.7195 Soluble
166 G H -0.5592 Soluble
167 A H -0.2810 Soluble
168 L H -0.0300 Soluble
169 T H -0.1676 Soluble
170 S H -0.3359 Soluble
171 G H -0.2298 Soluble
172 V H 0.1956 Aggregation-prone
173 H H 0.0000 Buried
174 T H -0.1144 Soluble
175 F H 0.0000 Buried
176 P H -0.3053 Soluble
177 A H 0.1970 Aggregation-prone
178 V H 0.4652 Aggregation-prone
179 L H 1.0261 Aggregation-prone
180 Q H 0.2293 Aggregation-prone
181 S H -0.1795 Soluble
182 S H -0.2432 Soluble
183 G H 0.0068 Aggregation-prone
184 L H -0.0171 Soluble
185 Y H 0.3128 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1154 Soluble
193 V H 0.0000 Buried
194 P H -0.5009 Soluble
195 S H -0.5470 Soluble
196 S H -0.6107 Soluble
197 S H 0.0000 Buried
198 L H -0.7462 Soluble
199 G H -0.9440 Soluble
200 T H -0.7270 Soluble
201 Q H -1.0331 Soluble
202 T H -1.0600 Soluble
203 Y H 0.0000 Buried
204 I H -1.2560 Soluble
205 C H 0.0000 Buried
206 N H -1.5325 Soluble
207 V H 0.0000 Buried
208 N H -2.0534 Soluble
209 H H 0.0000 Buried
210 K H -2.7957 Soluble
211 P H -1.6110 Soluble
212 S H -1.9071 Soluble
213 N H -2.6550 Soluble
214 T H -2.1733 Soluble
215 K H -2.7699 Soluble
216 V H -1.7688 Soluble
217 D H -2.6034 Soluble
218 K H -2.0350 Soluble
219 K H -2.3826 Soluble
220 V H 0.0000 Buried
221 E H -2.3305 Soluble
222 P H -1.1900 Soluble
1 D L -1.3551 Soluble
2 I L 0.0000 Buried
3 V L 0.7834 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.6099 Soluble
6 Q L 0.0000 Buried
7 S L -1.1532 Soluble
8 P L -1.3908 Soluble
9 D L -2.5165 Soluble
10 S L -1.6821 Soluble
11 L L -0.9447 Soluble
12 A L -0.7594 Soluble
13 V L 0.0000 Buried
14 S L -1.4344 Soluble
15 L L -0.6029 Soluble
16 G L -1.4229 Soluble
17 E L -2.9178 Soluble
18 R L -2.8496 Soluble
19 A L 0.0000 Buried
20 T L -0.6134 Soluble
21 I L 0.0000 Buried
22 N L -1.4563 Soluble
23 C L 0.0000 Buried
24 K L -1.6875 Soluble
25 S L 0.0000 Buried
26 S L -0.8421 Soluble
27 Q L -1.5414 Soluble
28 S L -1.0374 Soluble
29 L L 0.0000 Buried
30 L L -1.2128 Soluble
31 N L -1.9304 Soluble
32 R L -2.9094 Soluble
33 G L -2.2742 Soluble
34 N L -2.4284 Soluble
35 Q L -2.5928 Soluble
36 K L -1.6098 Soluble
37 N L -0.8455 Soluble
38 Y L 0.0269 Aggregation-prone
39 L L 0.0000 Buried
40 T L 0.0000 Buried
41 W L 0.0000 Buried
42 F L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.9270 Soluble
46 P L -1.4382 Soluble
47 G L -1.4599 Soluble
48 Q L -2.1278 Soluble
49 P L -1.4870 Soluble
50 P L 0.0000 Buried
51 K L -1.7800 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1465 Soluble
56 W L -0.0095 Soluble
57 A L 0.0000 Buried
65 S L -0.5643 Soluble
66 T L -0.5704 Soluble
67 R L -1.5396 Soluble
68 E L -1.4820 Soluble
69 S L -0.8871 Soluble
70 G L -1.0874 Soluble
71 V L 0.0000 Buried
72 P L -1.4866 Soluble
74 D L -2.3367 Soluble
75 R L -1.6405 Soluble
76 F L 0.0000 Buried
77 S L -0.9086 Soluble
78 G L -0.4861 Soluble
79 S L -0.7707 Soluble
80 G L -1.2831 Soluble
83 S L -1.1865 Soluble
84 G L -1.2699 Soluble
85 T L -1.7452 Soluble
86 D L -2.6309 Soluble
87 F L 0.0000 Buried
88 T L -0.9951 Soluble
89 L L 0.0000 Buried
90 T L -0.6804 Soluble
91 I L 0.0000 Buried
93 S L -1.6295 Soluble
94 L L 0.0000 Buried
95 Q L -1.2783 Soluble
96 A L -1.3229 Soluble
97 E L -1.9815 Soluble
98 D L 0.0000 Buried
99 V L -1.1908 Soluble
100 A L 0.0000 Buried
101 V L -1.0332 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 N L 0.0000 Buried
107 D L 0.3979 Aggregation-prone
108 Y L 0.5101 Aggregation-prone
109 T L 0.1637 Aggregation-prone
114 Y L 0.4275 Aggregation-prone
115 P L -0.3000 Soluble
116 L L 0.0000 Buried
117 T L 0.0183 Aggregation-prone
118 F L 0.0969 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.4662 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -2.2571 Soluble
124 L L 0.0000 Buried
125 E L -1.1953 Soluble
126 I L 0.0000 Buried
127 K L -1.7874 Soluble
128 R L -1.0812 Soluble
129 T L -0.2488 Soluble
130 V L 0.1576 Aggregation-prone
131 A L -0.1413 Soluble
132 A L -0.1643 Soluble
133 P L 0.0000 Buried
134 S L -0.1585 Soluble
135 V L 0.0000 Buried
136 F L 0.1201 Aggregation-prone
137 I L 0.1739 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5388 Soluble
140 P L -0.8091 Soluble
141 S L -1.5861 Soluble
142 D L -2.8580 Soluble
143 E L -2.7473 Soluble
144 Q L 0.0000 Buried
145 L L -2.1921 Soluble
146 K L -2.8148 Soluble
147 S L -1.7582 Soluble
148 G L -1.4366 Soluble
149 T L -1.1021 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.3495 Soluble
158 N L -0.8212 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7766 Soluble
162 R L -2.9174 Soluble
163 E L -3.1393 Soluble
164 A L -2.3243 Soluble
165 K L -2.4101 Soluble
166 V L 0.0000 Buried
167 Q L -0.7158 Soluble
168 W L 0.0000 Buried
169 K L -0.6436 Soluble
170 V L 0.0000 Buried
171 D L -2.0837 Soluble
172 N L -1.6533 Soluble
173 A L -0.2934 Soluble
174 L L 0.6904 Aggregation-prone
175 Q L -0.2799 Soluble
176 S L -0.6166 Soluble
177 G L -1.2041 Soluble
178 N L -1.4586 Soluble
179 S L -1.3607 Soluble
180 Q L -1.3426 Soluble
181 E L -1.5952 Soluble
182 S L -0.6710 Soluble
183 V L -0.7682 Soluble
184 T L -1.1504 Soluble
185 E L -2.3049 Soluble
186 Q L -1.8651 Soluble
187 D L -1.8565 Soluble
188 S L -1.9672 Soluble
189 K L -2.1987 Soluble
190 D L -1.6052 Soluble
191 S L -1.7119 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6577 Soluble
199 L L 0.0000 Buried
200 T L -0.6489 Soluble
201 L L -0.8438 Soluble
202 S L -1.1299 Soluble
203 K L -2.1158 Soluble
204 A L -1.8225 Soluble
205 D L -2.6823 Soluble
206 Y L 0.0000 Buried
207 E L -3.0441 Soluble
208 K L -3.4569 Soluble
209 H L -3.0482 Soluble
210 K L -3.2445 Soluble
211 V L -1.5493 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8124 Soluble
216 V L 0.0000 Buried
217 T L -1.2626 Soluble
218 H L 0.0000 Buried
219 Q L -1.7554 Soluble
220 G L -0.4771 Soluble
221 L L -0.2680 Soluble
222 S L -0.4653 Soluble
223 S L -0.4466 Soluble
224 P L -0.5975 Soluble
225 V L -0.0062 Soluble
226 T L -0.4442 Soluble
227 K L -0.5345 Soluble
228 S L -0.4889 Soluble
229 F L -0.9020 Soluble
230 N L -1.9835 Soluble
231 R L -2.5261 Soluble
232 G L -2.1346 Soluble
233 E L -2.5214 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6785 3.4911 CSV PDB
4.5 -0.7258 3.4476 CSV PDB
5.0 -0.7814 3.3929 CSV PDB
5.5 -0.8345 3.3319 CSV PDB
6.0 -0.873 3.2685 CSV PDB
6.5 -0.8881 3.2045 CSV PDB
7.0 -0.8801 3.1407 CSV PDB
7.5 -0.8568 3.0785 CSV PDB
8.0 -0.825 3.0203 CSV PDB
8.5 -0.7869 2.9691 CSV PDB
9.0 -0.7415 2.9268 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH