Izalontamab / 6axp

A4DAB149_6axp_D_C | Source PDB: 6axp | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6587
Max Score
2.0022
Min Score
-3.7182
Total Score
-279.94
Score CDR
-0.3064
Score non-CDR
-0.7014

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7182
Maximum Score 2.0022
Average Score -0.6587
Total Score -279.9426

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.3215 Soluble
2 V D -0.5583 Soluble
3 Q D -1.2449 Soluble
4 L D 0.0000 Buried
5 K D -2.2487 Soluble
6 Q D 0.0000 Buried
7 S D -0.8269 Soluble
8 G D -0.9711 Soluble
9 P D -0.4173 Soluble
11 G D -0.4260 Soluble
12 L D -0.0603 Soluble
13 V D 0.0000 Buried
14 Q D -1.3393 Soluble
15 P D -1.4026 Soluble
16 S D -1.5132 Soluble
17 Q D -1.9351 Soluble
18 S D -1.5478 Soluble
19 L D 0.0000 Buried
20 S D -0.5765 Soluble
21 I D 0.0000 Buried
22 T D -0.4888 Soluble
23 C D 0.0000 Buried
24 T D -1.1095 Soluble
25 V D 0.0000 Buried
26 S D -1.1000 Soluble
27 G D -0.8688 Soluble
28 F D -0.4944 Soluble
29 S D -0.8967 Soluble
30 L D 0.0000 Buried
35 T D -0.9298 Soluble
36 N D -0.8631 Soluble
37 Y D 0.4269 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4210 Soluble
45 S D -0.9640 Soluble
46 P D -1.1656 Soluble
47 G D -1.7508 Soluble
48 K D -2.2242 Soluble
49 G D -1.1237 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5094 Soluble
58 S D -0.6693 Soluble
59 G D -1.2145 Soluble
63 G D -1.5120 Soluble
64 N D -1.8247 Soluble
65 T D -1.2802 Soluble
66 D D -0.9695 Soluble
67 Y D -0.3568 Soluble
68 N D 0.0000 Buried
69 T D -0.1711 Soluble
70 P D -0.3705 Soluble
71 F D 0.0000 Buried
72 T D -0.4650 Soluble
74 S D -0.5578 Soluble
75 R D -0.9368 Soluble
76 L D 0.0000 Buried
77 S D -0.9604 Soluble
78 I D 0.0000 Buried
79 N D -1.7005 Soluble
80 K D -1.4532 Soluble
81 D D -1.8286 Soluble
82 N D -2.2902 Soluble
83 S D -1.7239 Soluble
84 K D -2.3942 Soluble
85 S D -1.6350 Soluble
86 Q D -1.5502 Soluble
87 V D 0.0000 Buried
88 F D -0.2089 Soluble
89 F D 0.0000 Buried
90 K D -1.3167 Soluble
91 M D 0.0000 Buried
92 N D -1.5230 Soluble
93 S D -1.4354 Soluble
94 L D 0.0000 Buried
95 Q D -2.1043 Soluble
96 S D -1.5215 Soluble
97 N D -2.0633 Soluble
98 D D 0.0000 Buried
99 T D -0.6399 Soluble
100 A D 0.0000 Buried
101 I D 0.5460 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.1455 Aggregation-prone
109 T D 1.1934 Aggregation-prone
110 Y D 1.8829 Aggregation-prone
111 Y D 1.9782 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.6587 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.3380 Aggregation-prone
117 Y D 0.3189 Aggregation-prone
118 W D -0.4233 Soluble
119 G D 0.0000 Buried
120 Q D -1.7831 Soluble
121 G D -0.8932 Soluble
122 T D 0.0000 Buried
123 L D 0.2488 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3237 Soluble
126 V D 0.0000 Buried
127 S D -0.5973 Soluble
128 A D -0.2603 Soluble
129 A D -0.2742 Soluble
130 S D -0.5071 Soluble
131 T D -0.7944 Soluble
132 K D -1.3437 Soluble
133 G D -1.4479 Soluble
134 P D -0.5995 Soluble
135 S D -0.2003 Soluble
136 V D -0.1529 Soluble
137 F D -0.3517 Soluble
138 P D 0.0000 Buried
139 L D 0.0000 Buried
140 A D -1.1537 Soluble
141 P D 0.0000 Buried
142 S D -0.7145 Soluble
143 G D -0.6421 Soluble
144 T D -0.4878 Soluble
145 A D -0.2519 Soluble
146 A D 0.0000 Buried
147 L D 0.0000 Buried
148 G D 0.0000 Buried
149 C D 0.0000 Buried
150 L D 0.0000 Buried
151 V D 0.0000 Buried
152 K D 0.0000 Buried
153 D D -0.4423 Soluble
154 Y D 0.0000 Buried
155 F D 0.0000 Buried
156 P D 0.0000 Buried
157 E D -0.4762 Soluble
158 P D -0.6789 Soluble
159 V D -0.7334 Soluble
160 T D -0.6747 Soluble
161 V D -0.3372 Soluble
162 S D -0.4793 Soluble
163 W D 0.0000 Buried
164 N D -0.8488 Soluble
165 S D -0.6891 Soluble
166 G D -0.5688 Soluble
167 A D -0.2397 Soluble
168 L D -0.0246 Soluble
169 T D -0.1877 Soluble
170 S D -0.1987 Soluble
171 G D -0.2472 Soluble
172 V D 0.1211 Aggregation-prone
173 H D -0.3159 Soluble
174 T D -0.0204 Soluble
175 F D 0.0000 Buried
176 P D -0.3699 Soluble
177 A D 0.1691 Aggregation-prone
178 V D 0.4501 Aggregation-prone
179 L D 1.0502 Aggregation-prone
180 Q D 0.2272 Aggregation-prone
181 S D -0.0746 Soluble
182 S D -0.2361 Soluble
183 G D 0.0016 Aggregation-prone
184 L D 0.0860 Aggregation-prone
185 Y D 0.3508 Aggregation-prone
186 S D 0.0000 Buried
187 L D 0.0000 Buried
188 S D 0.0000 Buried
189 S D 0.0000 Buried
190 V D 0.0000 Buried
191 V D 0.0000 Buried
192 T D -0.1456 Soluble
193 V D 0.0000 Buried
194 P D -0.5858 Soluble
195 S D -0.5447 Soluble
196 S D -0.6129 Soluble
197 S D -0.6061 Soluble
198 L D -0.8452 Soluble
199 G D -0.9840 Soluble
200 T D -0.7125 Soluble
201 Q D -1.2086 Soluble
202 T D -1.1511 Soluble
203 Y D 0.0000 Buried
204 I D -1.5080 Soluble
205 C D 0.0000 Buried
206 N D -1.6184 Soluble
207 V D 0.0000 Buried
208 N D -2.2240 Soluble
209 H D 0.0000 Buried
210 K D -2.6659 Soluble
211 P D -1.5754 Soluble
212 S D -1.8691 Soluble
213 N D -2.5469 Soluble
214 T D -2.1285 Soluble
215 K D -2.7951 Soluble
216 V D -1.7756 Soluble
217 D D -2.6699 Soluble
218 K D -2.3768 Soluble
219 R D -2.7479 Soluble
220 V D 0.0000 Buried
221 E D -2.7492 Soluble
222 P D -1.8399 Soluble
223 K D -2.2030 Soluble
1 D C -1.4847 Soluble
2 I C 0.0000 Buried
3 L C 0.5689 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.4050 Soluble
6 Q C -0.1746 Soluble
7 S C 0.0751 Aggregation-prone
8 P C 0.8669 Aggregation-prone
9 V C 2.0022 Aggregation-prone
10 I C 1.5695 Aggregation-prone
11 L C 0.5928 Aggregation-prone
12 S C -0.3553 Soluble
13 V C 0.0000 Buried
14 S C -1.9470 Soluble
15 P C -2.0886 Soluble
16 G C -2.1508 Soluble
17 E C -2.9858 Soluble
18 R C -3.0830 Soluble
19 V C 0.0000 Buried
20 S C -0.9170 Soluble
21 F C 0.0000 Buried
23 C C 0.0000 Buried
24 R C -2.4428 Soluble
25 A C -1.3606 Soluble
26 S C -1.0594 Soluble
27 Q C -1.7402 Soluble
28 S C -1.5963 Soluble
29 I C 0.0000 Buried
36 G C -1.0147 Soluble
37 T C -0.7205 Soluble
38 N C -0.7600 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.0974 Soluble
45 R C -1.7524 Soluble
46 T C -1.5902 Soluble
47 N C -1.7899 Soluble
48 G C -1.5038 Soluble
49 S C -0.9756 Soluble
50 P C 0.0000 Buried
51 R C -0.7545 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C 0.0000 Buried
56 Y C -0.4544 Soluble
57 A C 0.0000 Buried
65 S C -1.2422 Soluble
66 E C -1.9331 Soluble
67 S C -1.1405 Soluble
68 I C -0.4586 Soluble
69 S C -0.3132 Soluble
70 G C -0.4702 Soluble
71 I C -0.2623 Soluble
72 P C -0.3161 Soluble
74 S C -0.5364 Soluble
75 R C -1.0700 Soluble
76 F C 0.0000 Buried
77 S C -0.8878 Soluble
78 G C -0.7461 Soluble
79 S C -0.8819 Soluble
80 G C -0.9093 Soluble
83 S C -0.9477 Soluble
84 G C -1.3338 Soluble
85 T C -1.9053 Soluble
86 D C -2.4230 Soluble
87 F C 0.0000 Buried
88 T C -0.8619 Soluble
89 L C 0.0000 Buried
90 S C -0.8758 Soluble
91 I C 0.0000 Buried
92 N C -2.2336 Soluble
93 S C -2.2453 Soluble
94 V C 0.0000 Buried
95 E C -2.4177 Soluble
96 S C -2.3764 Soluble
97 E C -2.3123 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C 0.0000 Buried
101 D C 0.0000 Buried
102 Y C 0.0000 Buried
103 Y C -0.0547 Soluble
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.7051 Soluble
108 N C -1.3293 Soluble
109 N C -1.3993 Soluble
114 W C 0.0456 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C -0.0565 Soluble
118 F C 0.2212 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0902 Aggregation-prone
121 G C 0.2895 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.0981 Soluble
124 L C 0.0000 Buried
125 E C -1.2418 Soluble
126 L C 0.0000 Buried
127 K C -1.8085 Soluble
128 R C -1.2228 Soluble
129 T C 0.1245 Aggregation-prone
130 V C 0.9701 Aggregation-prone
131 A C 0.4615 Aggregation-prone
132 A C 0.0810 Aggregation-prone
133 P C 0.0000 Buried
134 S C -0.0606 Soluble
135 V C 0.0931 Aggregation-prone
136 F C 0.2213 Aggregation-prone
137 I C 0.1319 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5316 Soluble
140 P C -0.6090 Soluble
141 S C 0.0000 Buried
142 D C -2.3193 Soluble
143 E C -1.7898 Soluble
144 Q C 0.0000 Buried
145 L C -1.9103 Soluble
146 K C -2.4040 Soluble
147 S C -1.5072 Soluble
148 G C -1.1432 Soluble
149 T C -0.9421 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.8603 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.6350 Soluble
162 R C -3.0265 Soluble
163 E C -3.1989 Soluble
164 A C -2.2348 Soluble
165 K C -2.3762 Soluble
166 V C -1.0299 Soluble
167 Q C -0.6839 Soluble
168 W C 0.0000 Buried
169 K C -0.5948 Soluble
170 V C 0.0000 Buried
171 D C -1.5737 Soluble
172 N C -1.4102 Soluble
173 A C -0.2962 Soluble
174 L C 0.3546 Aggregation-prone
175 Q C -0.3044 Soluble
176 S C -0.4860 Soluble
177 G C -0.9002 Soluble
178 N C -0.8166 Soluble
179 S C -1.1984 Soluble
180 Q C -1.5378 Soluble
181 E C -2.1782 Soluble
182 S C -1.0819 Soluble
183 V C -1.1015 Soluble
184 T C -1.1182 Soluble
185 E C -2.1482 Soluble
186 Q C 0.0000 Buried
187 D C -1.9596 Soluble
188 S C -2.3543 Soluble
189 K C -2.6365 Soluble
190 D C -1.8087 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.7143 Soluble
199 L C 0.0000 Buried
200 T C -0.3513 Soluble
201 L C -0.7261 Soluble
202 S C -1.2969 Soluble
203 K C -2.1635 Soluble
204 A C -2.0540 Soluble
205 D C -2.6879 Soluble
206 Y C 0.0000 Buried
207 E C -3.7073 Soluble
208 K C -3.7182 Soluble
209 H C -3.1385 Soluble
210 K C -3.1050 Soluble
211 V C -1.3614 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.8376 Soluble
216 V C 0.0000 Buried
217 T C -1.0829 Soluble
218 H C 0.0000 Buried
219 Q C -1.5985 Soluble
220 G C -0.2283 Soluble
221 L C -0.1568 Soluble
222 S C -0.4508 Soluble
223 S C -0.4291 Soluble
224 P C -0.5057 Soluble
225 V C -0.0503 Soluble
226 T C -0.4877 Soluble
227 K C -0.6853 Soluble
228 S C -0.6799 Soluble
229 F C -0.9434 Soluble
230 N C -2.0535 Soluble
231 R C -2.4608 Soluble
232 G C -1.5189 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6898 2.7902 CSV PDB
4.5 -0.7239 2.7191 CSV PDB
5.0 -0.7646 2.6384 CSV PDB
5.5 -0.8035 2.554 CSV PDB
6.0 -0.8306 2.4685 CSV PDB
6.5 -0.8379 2.3826 CSV PDB
7.0 -0.8245 2.2971 CSV PDB
7.5 -0.7974 2.2128 CSV PDB
8.0 -0.7633 2.2031 CSV PDB
8.5 -0.7246 2.2012 CSV PDB
9.0 -0.6815 2.2487 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH